Uniform Criteria Research Articles

High-throughput solutions in tumor organoids: From culture to drug screening.

Tumor organoids have emerged as an ideal in vitro model for patient-derived tissues, as they recapitulate the characteristics of the source tumor tissue to a certain extent, offering the potential for personalized tumor therapy and demonstrating significant promise in pharmaceutical research and development. However, establishing and applying this model involves multiple labor-intensive and time-consuming experimental steps and lacks standardized protocols and uniform identification criteria. Thus, high-throughput solutions are essential for the widespread adoption of tumor organoid models. This review provides a comprehensive overview of current high-throughput solutions across the entire workflow of tumor organoids, from sampling and culture to drug screening. Furthermore, we explore various technologies that can control and optimize single-cell preparation, organoid culture, and drug screening with the ultimate goal of ensuring the automation and high efficiency of the culture system and identifying more effective tumor therapeutics.

Traditional Chinese Medicine treatment for postoperative axial symptoms of cervical spondylotic myelopathy: a systematic review

BackgroundAxial symptoms (AS) represent one of the primary complications after cervical spondylotic myelopathy (CSM) surgery. Traditional Chinese Medicine (TCM), as a complementary and alternative therapy, is widely used in the...

Liver Transplantation for the Cure of Neuroendocrine Liver Metastasis: A Systematic Review with Particular Attention to the Risk Factors of Death and Recurrence

Background/Objectives: Neuroendocrine neoplasms (NEN) are heterogeneous entities. Despite considerable advancement in the field, almost 50% of patients have metastatic disease, when liver transplantation (LT) is one of the possible treatments offering a cure in well-selected patients. Methods: The present study aims to systematically review all the literature from 2000 onwards on using LT for patients with NEN-LM, with particular attention to the risk factors of death and recurrence. Results: LT offers 5-year OS ranging from 52 to 74% and 5-year TFS rates ranging from 39 to 62%, with even better results published from 2009 onwards. The main risk factors for patient deaths are related to unfavorable primary tumor pathology, higher liver involvement, and simultaneous LT and primary resection. Similarly, recurrence is higher related to poor tumor grade and differentiation, and in the case of an older recipient age. Conclusions: Applying uniform criteria and a more in-depth understanding of the relevant prognostic factors contribute to a better selection of candidates for curative LT due to NEN metastases. LT for unresectable or liver-restricted NENs has a relevant place in the treatment algorithm and has achieved excellent results in recent decades, but more international efforts are needed to further improve outcomes.

Responsible Consumption: Developing Sustainable Habits of Living for The Future

This study looks at consumer behavior in a variety of socioeconomic circumstances with regard to sustainable consumption trends and variables. The study highlights the importance of personal beliefs, sociocultural elements, and outside influences by using a mixed-methods approach to uncover subtle patterns in consumer choices. To comprehend the intricacies of sustainable consumption, the theoretical framework incorporates socioeconomic variables, environmental psychology, and marketing methods. To collect both quantitative and qualitative data, the study makes use of questionnaires, interviews, and content analysis. The results emphasize how business sustainability activities and education play a part in influencing sustainable customer behavior. According to the research, there are still issues that need to be resolved, such as the absence of uniform sustainability criteria and more robust governmental interventions. The results highlight the significance of comprehensive approaches incorporating business, policy-driven, and educational activities for a sustainable future.

Directions and mechanisms of development of the common agrarian market of the Union State of Belarus and Russia

The article reveals trends in the development of food markets in Belarus and Russia, and features of the agricultural strategy. Proposals are presented for the development of mechanisms for the formation of a common agricul tural market of the Union State of Belarus and Russia, including measures of state regulation in the field of forecasting supply and demand (strategic indicative planning, infrastructure development), pricing (maintaining price parity and competitiveness), subsidies (application of uniform principles and criteria for increasing efficiency and competitiveness), investment policy (stimulating scientific and industrial cooperation, technology transfer), forming the basis for the functioning of market participants on the principles of voluntariness, economic feasibility and balance of interests.

Understanding Insulin Resistance in NAFLD: A Systematic Review and Meta-Analysis Focused on HOMA-IR in South Asians.

Non-alcoholic fatty liver disease (NAFLD), a metabolic condition, is becoming increasingly common in South Asia. While its clinical diagnosis primarily relies on sonography and altered hepatic biomarkers, the significance of non-hepatic indicators, such as Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), in relation to NAFLD requires further examination in the South Asian population due to ethnic differences in these markers. This study examined the relationship between insulin resistance, quantified using the Homeostasis Model Assessment of Insulin Resistance (HOMA-IR), and NAFLD, along with other non-hepatic biomarkers. A thorough literature review was conducted in accordance with the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. We searched the PubMed, Embase, and Google Scholar databases, yielding 287 articles. After applying the selection criteria and screening, 22 studies were selected for inclusion in the analysis. We extracted and meta-analyzed the data on HOMA-IR in patients with NAFLD, along with other relevant parameters. The Newcastle-Ottawa Scale (NOS) was used to assess the quality of observational studies, whereas the RoB 2.0 tool was employed for randomized controlled trials (RCTs). The systematic review uncovered that individuals with NAFLD demonstrated statistically significant elevations in HOMA-IR levels, with a weighted mean difference (WMD) of 1.28 (95% confidence interval (CI): 1.00-1.58, I² = 98%, p < 0.0001) when compared to healthy subjects. Additionally, NAFLD patients showed markedly higher fasting blood glucose (FBG) levels, with a combined mean difference of 15.64 mg/dL (95% CI: 11.03-20.25, I² = 92%, p < 0.0001). The analysis also revealed increased triglyceride levels in NAFLD patients, with a pooled mean difference of 42.49 mg/dL (95% CI: 29.07-55.91, I² = 97%, p < 0.0001), and elevated C-reactive protein (CRP) levels, with a pooled mean difference of 2.17 mg/L (95% CI: 2.01-2.33, I² = 23%, p < 0.0001). Interestingly, subgroup analysis indicated that obese NAFLD patients exhibited significantly higher HOMA-IR levels than their non-obese counterparts, with a weighted mean difference of 5.85 (95% CI: 4.88-6.81, I² = 0%, p < 0.0001). Variations in study methodology, diagnostic techniques, and subject demographics were identified as sources of heterogeneity. The analysis found little evidence of publication bias, which lends credibility to the results. In South Asian populations, higher HOMA-IR, triglyceride-glucose (TyG) index, and CRP levels are associated with an increased risk of NAFLD. To improve the understanding and treatment of NAFLD in this specific demographic group, it is necessary to establish uniform diagnostic criteria and conduct additional studies, particularly RCTs.

Predictors of drug-resistant epilepsy in childhood epilepsy syndromes: A subgroup analysis from a prospective cohort study.

Previous studies assessing factors associated with drug-resistant epilepsy (DRE) were constrained by their amalgamation of all epilepsy syndromes in their analyses and the absence of uniform criteria for defining DRE. Our objective was to identify predictors of DRE among the four primary childhood epilepsy syndrome groups within a cohort of children with new onset seizures, using the International League Against Epilepsy (ILAE) definition of DRE and the recent classification of epilepsies. This is a prospective study of 676 children with new onset seizures initiated on antiseizure medication. Patients were monitored for the occurrence of DRE according to the ILAE criteria and were categorized into one of four epilepsy groups: self-limited focal epilepsies (SeLFEs), genetic generalized epilepsies (GGEs), developmental epileptic encephalopathies (DEEs), and focal epilepsies. Cox regression analysis was performed to identify predictors of DRE within each epilepsy group. Overall, 29.3% of children were classified as having DRE, with the highest incidence observed among children diagnosed with DEEs (77.7%), followed by focal epilepsies (31.5%). Across the entire cohort, predictors of DRE included the presence of an epileptogenic lesion, a higher pretreatment number of seizures, experiencing multiple seizure types, presence and severity of intellectual and developmental delay, myoclonus, and younger age at epilepsy onset. Within the GGEs, only a younger age at seizure onset and experiencing multiple seizure types predicted DRE. Among focal epilepsies, predictors of DRE included the presence of an epileptogenic lesion, experiencing multiple seizure types, and having a greater number of pretreatment seizures. Within the DEEs, predictors of DRE were the occurrence of tonic seizures. Predictors of DRE within SeLFEs could not be identified. This study indicates that different epilepsy syndromes are associated with distinct predictors of drug resistance. Anticipation of drug resistance within various groups is feasible using accessible clinical variables throughout the disease course.

Bone graft substitutes used in anterior lumbar interbody fusion: a contemporary systematic review of fusion rates and complications.

Anterior lumbar interbody fusion (ALIF) uses a broad-footprint interbody cage designed to maximize fusion rates for treating degenerative disc disease. Bone graft substitutes are being increasingly utilized during ALIF to replace or supplement autologous iliac crest bone grafts. This approach aims to optimize fusion efficacy while minimizing associated postoperative complications. The objective of this systematic review was to examine recent studies on fusion rates and postoperative complications associated with bone graft substitutes used in ALIF. We conducted a systematic review of the Cumulative Index to Nursing and Allied Health Literature (CINAHL), Embase, MEDLINE, and PubMed databases, to critically examine a decade of research (January 1, 2012, to July 6, 2023) on the effectiveness and safety of various bone graft substitutes in ALIF. This timeframe was chosen to build on a previous systematic review published in 2013. The PRISMA guidelines were used. In total, 27 articles met our stringent inclusion and exclusion criteria. A substantial portion of these studies (67%) focused on recombinant human bone morphogenetic protein-2 (rhBMP-2) and highlighted its efficacy for achieving high fusion rates. However, the literature presents a dichotomy regarding the association of rhBMP-2 with increased postoperative complications. Notably, the methodologies for evaluating spinal fusion varied across studies. Only one-third of studies employed computed tomography to assess interbody fusion at 12 months postoperatively, highlighting the urgent need to establish uniform fusion criteria to facilitate more accurate comparative analyses. Moreover, there was considerable variability in the criteria used for diagnosing and detecting postoperative complications, significantly influencing the reported incidence rates. This review underscores the need for continued research into bone graft substitutes, particularly focusing on assessment of long-term complications. Future research endeavors should concentrate on developing comprehensive clinical guidelines to aid in the selection of the most suitable bone graft substitutes for use in ALIF, thereby enhancing patient outcomes and surgical efficacy.

КРИТЕРИИ ЭФФЕКТИВНОСТИ ЛЕЧЕНИЯ ПАЦИЕНТОВ С МИОФАСЦИАЛЬНЫМ БОЛЕВЫМ СИНДРОМОМ ЖЕВАТЕЛЬНОЙ МУСКУЛАТУРЫ

There are quite a lot of predictor factors for the development of myofascial pain syndrome of the chewing muscles. At the moment, there is no single pathogenetic theory of the development of this pathology. Accordingly, uniform criteria for evaluating the effectiveness of the therapy have not been developed, which was the purpose of this study. The aim of the study is to develop and implement criteria for the effectiveness of treatment of patients with myofascial masticatory muscle pain syndrome. Materials and methods. 45 people with myofascial masticatory muscle pain syndrome were examined and treated. Screening diagnostics of 30 conditionally healthy people was carried out to obtain control values. The study was conducted in three stages – assessment of stress levels according to the data of filling out a questionnaire on the level of susceptibility to stress, clinical examination – determination of qualitative and quantitative criteria, ultrasound and electromyographic examination. Relief of myofascial pain syndrome of the masticatory muscles was performed by injections of botulinum toxin type A into the masticatory and temporal muscles. The dynamics was assessed at the time of 14 days and 1 month. Results. The developed criteria clearly reflect the effectiveness of the therapy. Statistically significant results of the effectiveness of treatment have been obtained, allowing timely optimal therapy and preventing the development of chronic disorders of the temporomandibular joint. Understanding the combined influence of the psychoemotional state and somatic factors led to the development of uniform criteria for the effectiveness of treatment of this category of patients. The analysis and dynamics of changes in qualitative and quantitative indicators reflect the current trend towards the development of minimally invasive approaches to diagnosis and treatment based on the principles of biofeedback, which prevent the chronization of the process and the development of temporomandibular joint dysfunction.

Issues of Determining the Systematicity in the Qualification of Crimes

The article analyzes the issues of determining the systematicity in the qualification of criminal offenses. Based on the conducted research, the author concludes that there are no uniform criteria for determining the evaluation feature — systematicity, quantitative and temporal characteristics. The author shows that the systematicity is determined differently in different types of crimes, in particular in the article, in the note to the article or in the explanations of the Resolutions of the Plenum of the Supreme Court of the Russian Federation. The examples given in the paper show striking differences in criteria. So, systematic can be suitable: at least once, more than twice, or three or more times. By time, this may be at least once a quarter, during the year, during the period of the prescribed measures, or there are no specific time limits. The use of analogy according to the norms of the Criminal Code of the Russian Federation is prohibited, and there are not always norms defining the criteria for the qualification of evaluative concepts. The conducted research allows us to conclude that it is necessary to develop unified approaches to the interpretation of the norms of legislation on systematization and their consolidation in criminal legislation.

Репрезентативность показателей и объективность оценок в компаративистике: экономика Российской империи второй половины XIX столетия в современных зарубежных исследованиях

The relevance of comparative studies of the level of industrial and economic development of the Russian Empire in the second half of the XIX century is determined by the fact that their results in some cases are used to substantiate theoretical ideas about the national characteristics of the Russian economy and society in comparison with the leading countries of Industrial Revolution. In economic literature, assertions about the “catastrophic” backwardness of Russia during this period are often encountered. Modern foreign historical-economic research allows for a more objective assessment of the gap between the level of industrial and economic development of the Russian Empire and the “advanced” states. However, the authors of these studies do not always succeed in consistently applying the principle of uniform criteria for comparing different economies. In particular, it is hardly justified: in some cases, to take into account, and in others, not to take into account the population and economic potential of territorial isolated peripheral regions of the respective economies. For example, the value of historical and economic comparisons is reduced by the confusion in research between the actual territory of the Russian Empire in the middle and second half of the XIX century, its territory within the conditional “borders of 1913” and the territory within “former USSR” borders. In this regard the article notes that conclusions drawn on the basis of fragmentary and arbitrarily interpreted information can’t be considered as fully correct. According to the author, in a comparative analysis of the economies-leaders of the Industrial Revolution, it is necessary to use assessment methods that take into account the population and indicators of economic development of peripheral territories were included in trade and financial exchanges with these economies, because this inclusion strongly spired the development of economies-leaders.

Treatment of acute and chronic graft-versus-host disease with extracorporeal photopheresis: Update of best practice recommendations from Italian Society of Hemapheresis and Cell Manipulation (SIdEM) and the Italian Transplant Group for Bone Marrow Transplantation, Hematopoietic Stem Cells and Cell Therapy (GITMO)

These guidelines represent a GRADE-method revision of the recommendations produced by the Italian Society of Hemapheresis and Cell Manipulation (SIDEM) and the Italian Transplant Group for Bone Marrow Transplantation, Hematopoietic Stem Cells and Cell Therapy (GITMO) in 2013. Since 2013 several studies have been published that have strengthened the role of ECP in the management of GVHD. Thus, it was deemed appropriate to proceed with an update, with the aim to define uniform criteria for the application of ECP in adult and pediatric patients affected by GVHD throughout the national territory, in line with international guidelines, in maintaining of high standards of safety for patients and quality of the procedures provide. Post-HSCT GvHD therapies other than ECP and ECP therapy of other diseases, such as CTCL, are not covered by these guidelines.The development panel for this guideline includes professionals from various specialties who routinely interact in the management of the patient with GVHD, namely the transfusionist, the adult and pediatric hematologist, and the hospital pharmacist. A hematologist experienced in systematic reviews and GRADE guideline development ccordinated the development process, and an experienced transfusionist coordinated the assignment of tasks and reporting. External reviewers of the guideline included a patient representative.

Global trend and epidemiological profiles of climate-related disasters from 2000 to 2021.

The objective of this study is to analyse the epidemiological profile of global climate-related disasters in terms of morbidity and mortality, as well as to examine their temporal trends. This cross-sectional study analysed climate-related global disasters from 2000 to 2021, utilising definitions and criteria from the United Nations Strategy for Disaster Reduction and the Centre for Research on the Epidemiology of Disasters. Data were sourced from the EM-DAT database. The study assessed trends over the entire period and compared them with previous years (1978-2000). A total of 7398 climate-related disasters were recorded, with hydrological disasters being the most frequent, followed by meteorological and climatological disasters. Statistically significant differences were noted in the average rates of affected individuals and injuries per million inhabitants. No significant trends were found in mortality rates, but the frequency trends for the entire period (1978-2021) and the subperiod (1978-2000) were increasing and statistically significant. However, the trend from 2000 onwards showed a non-significant decrease, potentially reflecting better disaster preparedness and response strategies under the Hyogo and Sendai Framework. The study highlights hydrological disasters as the most frequent and deadliest climate-related events, with climatological disasters affecting and injuring the most people. The lack of standardised criteria for disaster inclusion in databases presents a significant challenge in comparing results and analysing trends. Establishing uniform inclusion criteria is crucial for effective data analysis and disaster management.

Advances, recognition, and interpretation of molecular heterogeneity among conventional and subtype histology of urothelial carcinoma (UC): a survey among urologic pathologists and comprehensive review of the literature.

Urothelial carcinoma (UC) demonstrates significant molecular and histologic heterogeneity. The WHO 2022 classification has hinted at adding molecular signatures to the morphologic diagnosis. As morphology and associated molecular repertoire may potentially translate to choices of and response to therapy and relapse rate, broader acceptability of recognizing these key features among uropathologists is needed. This prompted an international survey to ascertain the practice patterns in classical/subtype UC among uropathologists across the globe. A survey instrument was shared among 98 uropathologists using SurveyMonkey software. Anonymized respondent data were analysed. The response rate was 85%. A majority were in concordance with the profiles of luminal (93%) and basal (82%) types. Opinion on the FGFR3 testing platform was variable. While 95% concurred that TERT promoter mutation is the key driver in UC, 72% had the opinion that APOBEC mutagenesis is the main signature in muscle invasive bladder cancer (MIBC). Uropathologists have divergent opinions on MIBC and ERCC2 mutations. Among the participants, 94% would quantify aggressive micropapillary and sarcomatoid histology, while 88% would reevaluate another transurethral resection of the bladder tumour specimen in nonmuscle invasive tumour with micropapillary, small cell, or sarcomatoid histology. A leading number agreed to specific molecular signatures of micropapillary (93%), plasmacytoid (97%), and small cell (86%) subtypes. Ninety-six percent of participants agreed that a small-cell component portends a more aggressive course and should be treated with neoadjuvant chemotherapy and 63% would perform HER2/neu testing only on oncologist's request in advanced tumours. Ninety percent agreed that microsatellite instability testing, although not a standard protocol, should be considered in young patients with upper tract UC. Eighty-six percent agreed that UC with high tumour mutational burden would be a better candidate for immunotherapy. In the era of precision medicine, enhanced understanding of molecular heterogeneity of UC will contribute to better therapeutic options, novel biomarker discovery, innovative management protocols, and outcomes. Our survey provides a broad perspective of pathologists' perceptions and experience regarding incorporation of histomolecular approaches to "personalize" therapy. Due to variable clinical adoption, there is a need for additional data using uniform study criteria. This will drive generation of best practice guidelines in this area for widespread and consistent clinical utility.

The Construction and Application of Smart Classrooms in China: A Literature Review Based on 93 Studies

The smart classroom, as a product of advances in information technology, has garnered wide attention in academia in recent years. Using the method of literature review, this article delves into the construction and application of smart classrooms in China on the basis of 93 prior studies retrieved from the database of the China National Knowledge Infrastructure. Research results show that there is a lack of uniform evaluation criteria for the construction of smart classrooms despite the prominent advancements in their systems and facilities, and that in the dimension of applications, there has been growing interest in the alignment between smart classroom technologies and instructional needs but a dearth of research on teaching activity design for smart classroom instruction. Recommendations are also proposed based on the issues detected, with a view to contributing to the widespread deployment and ongoing optimization of smart classrooms.

Accuracy of the national violent death reporting system in identifying unintentional firearm deaths to children by children

BackgroundIn assigning manner of death (MOD) for inclusion on death certificates, medical examiners and coroners do not always apply uniform criteria. Previous research indicates surveillance statistics based on death certificates, such as the National Vital Statistics System, grossly miscount unintentional firearm deaths. The National Violent Death Reporting System (NVDRS) has taken steps to reduce variability in manner of death coding by providing uniform criteria for assigning an “abstractor manner of death” (AMD). AMD has five categories: unintentional, suicide, homicide, undetermined, and legal intervention homicide. A previous study found good accuracy of AMD coding for unintentional firearm deaths, all ages, 2003–2006, but a more recent study reported that the NVDRS undercounted self- and other-inflicted unintentional firearm deaths in which both the victim and shooter (for other-inflicted injuries) were under age 15 (2009–2018).FindingsWe replicated the recent study’s sample population, identifying 924 NVDRS incidents from 2009 to 2018 in which both victim and, for other-inflicted injuries, shooter age was under 15 and AMD was homicide, suicide, unintentional or undetermined (there were no legal intervention deaths to children). We assigned a researcher-adjudicated MOD (RMD) by reviewing incident narratives. RMD was compared with AMD and with manner recorded on the death certificate. Based on RMD as the gold standard, the sensitivity, specificity, and predictive values positive and negative of the AMD for unintentional childhood firearm deaths were, respectively, 90%, 99%, 98% and 96%; 86% (24/28) of false negatives were coded by abstractors as homicides. By contrast, death certificate manner had relatively poor sensitivity (63%).ConclusionsIn our sample of 924 deaths, the abstractor manner of death generally agreed with researcher-adjudicated manner of death, though not perfectly, missing 10% of researcher-adjudicated unintentional deaths, mostly because abstractors coded these unintentional deaths as homicides. A sizable minority of false negatives were unintentional deaths where the narrative explicitly noted that adult negligence contributed to a child’s unintentional shooting death. While AMD coding in NVDRS is good, it could be improved if NVDRS coding guidelines explicitly affirmed that potential prosecution for negligent manslaughter is not a contraindication to an AMD of unintentional, provided the firearm was not used to intentionally harm, threaten, or coerce.

Characterization and mapping of continental breakup and seafloor spreading initiation: The example of the northern rifted margin of the South China Sea

AbstractMapping ocean‐continent transitions (OCTs) separating equivocal continental and oceanic crusts is fundamental to investigate breakup processes and define the age and location of initial seafloor spreading. However, proposed limits of OCTs are rarely consistent, do not use uniform criteria, and result in conflicting interpretations as shown for the case of the northern South China Sea (SCS). We review original datasets including reflection and refraction seismic sections, drilling and potential field data with the aim to develop a ‘drilling‐constrained integrated geological‐geophysical’ approach to define the OCT along the northern SCS, understand the breakup process, and to compare the OCT in the SCS with those at Atlantic type rifted margins. The result shows a narrow, 5–15 km wide OCT. It separates a segmented margin that rifted a former arc in the west and a forearc in the east, both facing a Penrose oceanic crust that thins from the west towards the east. Seafloor spreading may have first nucleated at two centres during magnetic anomaly C11 in the NE and central subbasins, which then locally propagated both W and E to break through salients and produce full breakup at 29 Ma (anomaly C10r). Breakup at the SCS shows many differences to Atlantic type margins, in part due to inheritance but also due to rift/spreading‐related parameters such as strain/spreading rates.

Study of prerequisites for the formation of systematically important banks in regional economic associations

Despite the fact that more than ten years have passed since the rules for domestic systemically important banks (D-SIBs) were developed, the issues of identification of systemically important banks (SIB) and their regulation remain relevant today. Of particular interest is the possibility of forming uniform criteria for identifying systemically important banks within the framework of regional economic associations. The study is aimed at studying the prerequisites and fundamental possibilities of creating common criteria for classifying banks of the participating countries (Kazakhstan, Belarus, Russia) as SIB at the level of the Eurasian Economic Union (EAEU) through a clusterization approach. Clustering methodologies using Euclidean distance metrics and the Hamming model allowed for an initial study on the potential of the participating countries to develop common criteria for the SIB of the EAEU Union. The results of the analysis showed that the participating countries have the potential and some prerequisites for the development of common criteria within the union. However, it was found that the systemically important banks of the participating countries often change the level, since the criteria are not stable. Therefore, when forming uniform criteria, this point should be taken into account and criteria should be developed that are not approximated with each other, and similar features are significantly distinctive. The study consists of two parts, this work is the first part.

Inflammaging and prognostic markers of endometriosis

Inflammaging, an age-associated inflammation, is a cellular stress response caused by DNA damage, activation of oncogenes or inactivation of tumor suppressors, oxidative stress, chemotherapy, mitochondrial dysfunction, or epigenetic changes. Damage to macromolecules leads to the cessation of proliferation due to the activation of pathways such as p53/p21CIP1 and p16INK4a/RB. These form the senescence-associated secretory phenotype (SASP), the molecular/cellular manifestations of which in endometrial cells have features similar to those observed in endometriosis. Presently, there are no uniform diagnostic criteria or established molecular markers that can predict the development and course of endometriosis. In this regard, it is relevant to develop new minimally invasive examination methods, statistically based criteria and molecular markers for early diagnosis and prognosis of endometriosis. This review article is devoted to identifying molecular markers that characterize the pathogenesis of endometriosis during inflaming. The aim of the study was to consider modern ideas about the mechanisms of inflaming and its role in the development of endometriosis to determine possible molecular markers for predicting the course of the pathology. We used the PubMed, Scopus and Google Scholar databases to analyze and systematize the literature over the past ten years. Our review reflects the main molecular mechanisms and prognostic criteria that characterize the development of endometriosis during inflaming.

Hernia diafragmática congénita: relación entre el tamaño del defecto y evolución. Experiencia en un centro de referencia

IntroductionCongenital diaphragmatic hernia (CDH) remains a therapeutic challenge. The surgical classification recommended by the Congenital Diaphragmatic Hernia study group (CDHSG), based on the size of the defect, is used for staging in reference centres. Larger defects are associated with poorer outcomes. Our aim was to describe and compare the morbidity at hospital discharge of newborns who underwent surgical correction of CDH at the Juan P. Garrahan, according to the surgical staging of the defect proposed by the CDHSG. Material and methodsThe study included patients with CDH admitted to the Juan P. Garrahan Hospital between 2012 and 2020, and we analysed the distribution, morbidity and mortality associated with the size of the defect. We carried out a descriptive analysis, calculating measures of central tendency and dispersion, and bivariate and multivariate analyses. ResultsA total of 230 patients with CDH were admitted and 158 underwent surgery. We found that defect sizes C and D sizes were associated with an increased risk of chronic pulmonary disease (CPD) (OR, 5.3; 95% CI, 2.2-13.4; P<.0000), need of extracorporeal membrane oxygenation (OR 3.9; 95% CI, 1.3-12.8; P<.005) and chylothorax (OR, 2.1; 95% CI, 0.8- 6.4; P<.10]. The multivariate analysis revealed that a large defect size (C-D) was independently and significantly associated with CPD (OR 4.19; 95% CI, 1.76-9.95). ConclusionStaging the defect according to de CDHSG classification during surgery allows the application of uniform management criteria and the prediction of patient outcomes and complications during the hospital stay.