Unidirectional Migration Research Articles

A Unique Intramolecular Redox Reaction by Unidirectional Migration of Three Hydrogen Atoms: Theoretical Elucidation of the 3H-Transfer Sequence.

The intramolecular migration of three hydrogen atoms from one moiety of a gaseous radical cation to the other prior to fragmentation is an extremely rare type of redox reaction. Within the scope of this investigation, this scenario requires an ionized but electron-rich arene acceptor bearing a para-(3-hydroxyalkyl) residue. The precise mechanism of such unidirectional 3H transfer processes, including the order of the individual H transfer steps, has remained unclear in spite of previous isotope labelling and recent infrared ion spectroscopy (IRIS) studies. Herein, the details of this peculiar process have been investigated for ionized 4-(N,N-dimethylaminophenyl)-2-butanol, 2, by state-of-the-art density functional theory (DFT) calculations. The energetically most favorable pathway consists of a sequence of successive 1,4-, 1,6- and 1,5-H steps. During these steps the secondary alcohol functionality is oxidized to a carbonyl group and the radical-cationic aniline ring is reduced to an ionized 2,3-dihydroaniline unit. Several alternative sequences, such as three successive 1,5-H shifts, could be excluded. A concomitant unidirectional 2H migration reaction of the ion 2 was also investigated and the intermediacy of ion-neutral complexes (INCs) enabling sequential hydride and proton transfer was confirmed by the calculations.

Single nucleotide polymorphism (SNP) analysis reveals ancestry and genetic diversity of cultivated and wild grapevines in Croatia

BackgroundCroatia is a geographically small country with a remarkable diversity of cultivated and spontaneous grapevines. Local germplasm has been characterised by microsatellite markers, but a detailed analysis based on single nucleotide polymorphisms (SNPs) is still lacking. Here we characterize the genetic diversity of 149 accessions from three germplasm repositories and four natural sites using 516,101 SNPs to identify complete parent-offspring trios and their relations with spontaneous populations, offering a proof-of-concept for the use of reduced-representation genome sequencing in population genetics and genome-wide association studies (GWAS).ResultsPrincipal component analysis revealed a clear discontinuity between cultivated (V. vinifera subsp. sativa) and spontaneous grapevines, supporting the notion that the latter represent local populations of the wild progenitor (V. vinifera subsp. sylvestris). ADMIXTURE identified three ancestry components. Two sativa components are alternatively predominant in cultivars grown either in northern Adriatic Croatia and Continental Croatia or in Dalmatia (i.e. central and southern Adriatic Croatia). A sylvestris component, which is predominant in accessions from spontaneous populations, is a minor ancestry component in cultivated accessions. TREEMIX provided evidence of unidirectional migration from the vineyards to natural sites, suggesting that gene flow has gone preferentially from the introduced domesticated germplasm into local wild populations rather than vice versa. Identity-by-descent analysis indicated an extensive kinship network, including 14 complete parent-offspring trios, involving only cultivated accessions, six full-sibling relationships and invalidated a presumed pedigree of one of the most important varieties in Croatia, ‘Plavac Mali’. Despite this strong population structure, significant association was found between 143 SNPs and berry skin colour and between 2 SNPs and leaf hairiness, across two previously known genomic regions.ConclusionsThe clear genetic separation between Croatian cultivars and sylvestris ruled out the hypothesis that those cultivars originated from local domestication events. On the other hand, the evidence of a crop-to-wild gene flow signals the need for an urgent adoption of conservation strategies that preserve the residual genetic integrity of wild relatives. The use of this reduced-representation genome sequencing protocol in grapevine enables an accurate pedigree reconstruction and can be recommended for GWAS experiments.

Achieving "Ion Diode" Salt Resistance in Solar Interfacial Evaporation by a Tesla Valve-Like Water Transport Structure.

Salt deposition is a disturbing problem that limits the development of passive solar-driven interfacial evaporation. Inspired by the passive fluid control mechanism of the Tesla valve, a novel solar evaporator is proposed with a Tesla valve-like water transport structure to prevent salt accumulation at the evaporation interface. A unique "ion diode" salt resistance of this evaporator is significantly achieved by optimizing the two asymmetric water transport structures, consisting of one Tesla valve-like side and one wide-leg side, which establish a reverse-suppressing and forward-accelerating water transport channel. In contrast to the limited ion migration of the typical symmetric solar evaporator, such a channel caused by the water/salt ions transport difference between two water supply structures, reinforces the water/salt ions supply on the wide-leg side, thus leading to an apparent unidirectional salt ions migration from the wide-leg side to bulk water through the Tesla valve-like side. Consequently, an evaporation rate of 3.25kg m-2h-1 and a conversion efficiency of 83.27% under 2suns are achieved in 16wt% NaCl solution. The development of the Tesla Valve-like evaporator provides a new perspective for solving salt deposition and realizing scalable applications of solar-driven interfacial evaporation.

Unidirectional dispersal of blow fly larvae following decomposition fluids from a pig carcass

The decomposition of a body, and the associated gaseous and liquid discharges emanating from it, attract gravid female blow flies which lay their eggs in or on the body. After the eggs have hatched, the emerging larvae start feeding on the body. As decomposition progresses, the blow fly larvae often migrate away, typically in a random manner in search of favourable conditions for pupation. In this paper, we report on a rarely described phenomenon of unidirectional mass migration of blow fly larvae and postulate on the factors which may drive this process. A decomposition trial utilising a 60-kg pig carcass, deployed in the summer months in Table Mountain National Park, Cape Town, South Africa, was conducted in 2022. On the fifth day of the trial, simultaneous unidirectional mass dispersal of blow fly larvae was observed. The larvae moved downhill in a southeasterly direction, following the flow of decomposition fluids oozing out from the pig carcass. The ‘larval migration stream’ had a length of approximately 1.5 m with a width of 40 cm, tapering to 17 cm at the terminal point. The larval migration stream consisted of the larvae of Chrysomya albiceps and Chrysomya chloropyga. This study demonstrates the importance of understanding the timing and pattern of dispersal of post-feeding blow fly larvae in each geographical region. This is crucial as the minimum post-mortem interval can be miscalculated if older immature insects dispersing from the corpse are not considered and collected during crime scene investigations.

Organofluorosilicon Modified Polyacrylate with the Unidirectional Migration Promotion of Disperse Dyes toward Polyester Fabric for Wash-Free Digital Inkjet Dyeing.

The printing and dyeing industry is currently accelerating toward a direction of high efficiency, energy conservation, environmental protection, and integration with digitalization. Disperse dye wash-free digital inkjet dyeing is a revolutionary breakthrough for cleaning and coloring polyester fabric. Based on the solubility parameters and the hot-melt dyeing characteristics of disperse dyes, soft, hard, and functional monomers of acrylate were used as the main body. Moreover, single-vinyl fluorinated polysiloxane and divinyl polysiloxane with low solubility parameters were used as modified monomers. A modified polyacrylate (PFSMA) adhesive containing silicon in the main chain and fluorine silicon in the side chain was prepared via miniemulsion polymerization. Using disperse digital inkjet dyeing of polyester fabric without washing can realize energy saving, emission reduction, and carbon reduction. Results showed that the optimum preparation conditions of PFSMA were as follows: DVFS molecular weight of 957 g/mol and DVFS content of 2.5 wt %. Compared with that of polyacrylate (PA), the glass-transition temperature of PFSMA film decreased, and its water resistance, toughness, and adhesion enhanced. When the PFSMA content in the wash-free disperse red ink was 8 wt %, the color yields of the front and back of the PFSMA jet-dyed polyester fabric were 18.86 and 13.28, respectively. Moreover, the color yield of the front of PFSMA jet-dyed polyester fabric was 39.9% higher than that of the pure liquid disperse red jet-dyed fabric. The simulated fixation rate was 87.9%, approximately 2.9 times higher than that of the PA wash-free jet-dyed fabric. The color fastness to dry rubbing reached level 4 and the color fastness to wet rubbing reached level 3-4, which was one level higher than that of pure liquid disperse red jet-dyed fabrics. The color fastness to soaping reached grade 5 and the color fastness to heat compression reached grades 4-5 and above. The fabric was a little firmer but smoother. The color properties, color fastness, and hand feeling of the PFSMA wash-free jet-dyed polyester fabric exceeded the levels of commercially available adhesives.

Nonlinear compartmental modeling to monitor ovarian follicle population dynamics on the whole lifespan.

In this work, we introduce a compartmental model of ovarian follicle development all along lifespan, based on ordinary differential equations. The model predicts the changes in the follicle numbers in different maturation stages with aging. Ovarian follicles may either move forward to the next compartment (unidirectional migration) or degenerate and disappear (death). The migration from the first follicle compartment corresponds to the activation of quiescent follicles, which is responsible for the progressive exhaustion of the follicle reserve (ovarian aging) until cessation of reproductive activity. The model consists of a data-driven layer embedded into a more comprehensive, knowledge-driven layer encompassing the earliest events in follicle development. The data-driven layer is designed according to the most densely sampled experimental dataset available on follicle numbers in the mouse. Its salient feature is the nonlinear formulation of the activation rate, whose formulation includes a feedback term from growing follicles. The knowledge-based, coating layer accounts for cutting-edge studies on the initiation of follicle development around birth. Its salient feature is the co-existence of two follicle subpopulations of different embryonic origins. We then setup a complete estimation strategy, including the study of structural identifiability, the elaboration of a relevant optimization criterion combining different sources of data (the initial dataset on follicle numbers, together with data in conditions of perturbed activation, and data discriminating the subpopulations) with appropriate error models, and a model selection step. We finally illustrate the model potential for experimental design (suggestion of targeted new data acquisition) and in silico experiments.

Migration and controls of Shenhu submarine canyons in the upper continental slope of northern South China Sea: Insights from three‐dimensional seismic data mapping

AbstractSubmarine canyons, prominent features on continental margins, hold significant scientific and engineering implications. Despite extensive research on various types of submarine canyons, current understanding of those canyons developed on the upper continental slope, recognized as the most common type globally, remains limited. The Shenhu Canyons on the upper continental slope of the northern South China Sea, comprising over 19 subparallel features persistently developed from the Late Miocene to Quaternary, offer an excellent opportunity for in‐depth study. Previous assumptions suggested a unidirectional eastward migration of these canyons parallel to the host margin, primarily based on observations from two‐dimensional transverse seismic profiles. However, this study's analysis using three‐dimensional seismic data mapping reveals that not all canyons have migrated eastward; 28% display an opposite or a zigzag patterned migration, challenging previous assumptions. The eastward migration of the Shenhu Canyons on the eastern slope of the palaeo‐Pearl River shelf‐edge delta can be linked to the south‐east–southward progradation of the host margin and the palaeo‐shelf‐edge delta. However, the overall eastward migration pattern is complicated by submarine landslides and turbidity current processes. Landslides can influence the direction and magnitude of canyon migration through four mechanisms: turbidity current diversion; retrogressive development of canyon‐wall landslides; retrogressive landslides on surrounding open slopes; and emplacement of landslides in canyon thalwegs. These mechanisms may result in widening, bifurcation, confluence or obstruction of canyons, contributing to canyon migration. Erosion by turbidity currents can trigger canyon‐wall failures, which in turn promote the lateral migration of the canyons. If overspilled turbidity currents lead to the growth of levées in the lower reaches, canyon confinement increases, suppressing lateral migration. The inherited high‐gradient Palaeogene syn‐rift tectonic slope, combined with the depositional delta‐front slope of the palaeo‐shelf‐edge delta, creates favourable conditions for the initiation and persistent development of the straight Shenhu Canyons. This comprehensive analysis provides valuable insights into the complex dynamics and factors influencing the migration of upper continental slope submarine canyons.

Biointerfaces with ultrathin patterns for directional control of cell migration

In the context of wound healing and tissue regeneration, precise control of cell migration direction is deemed crucial. To address this challenge, polydimethylsiloxane (PDMS) platforms with patterned 10 nm thick TiOx in arrowhead shape were designed and fabricated. Remarkably, without tall sidewall constraints, MC3T3-E1 cells seeded on these platforms were constrained to migrate along the tips of the arrowheads, as the cells were guided by the asymmetrical arrowhead tips which provided large contact areas. To the best of our knowledge, this is the first study demonstrating the use of thin TiOx arrowhead pattern in combination with a cell-repellent PDMS surface to provide guided cell migration unidirectionally without tall sidewall constraints. Additionally, high-resolution fluorescence imaging revealed that the asymmetrical distribution of focal adhesions, triggered by the patterned TiOx arrowheads with arm lengths of 10, 20, and 35 μm, promoted cell adhesion and protrusion along the arrowhead tip direction, resulting in unidirectional cell migration. These findings have important implications for the design of biointerfaces with ultrathin patterns to precisely control cell migration. Furthermore, microelectrodes were integrated with the patterned TiOx arrowheads to enable dynamic monitoring of cell migration using impedance measurement. This microfluidic device integrated with thin layer of guiding pattern and microelectrodes allows simultaneous control of directional cell migration and characterization of the cell movement of individual MC3T3-E1 cells, offering great potential for the development of biosensors for single-cell monitoring.

High effects of climate oscillations on population diversity and structure of endangered Myricaria laxiflora.

Exploring the effects of climate oscillations on the population diversity and structure of endangered organisms in the Three Gorges Reservoir (TGR) area is essential for hydrological environment changes on endangered organism evolution. Myricaria laxiflora is an endemic and endangered shrub restricted to the TGR along the banks of Yangtze River, China. Recently, six natural populations of this species were newly found upstream and downstream of the TGR, whose habitats have been dramatically changed by the summer flooding regulated by large dams. To study the water level fluctuations and climatic shifts on the genetic diversity and genetic differentiation of the six natural populations, 303 individuals from six populations were analyzed based on one nuclear DNA (ITS) and four chloroplast fragments (trnL-F, psbA-trnH, rps16, and rpl16). The phylogenetic tree and significant genetic divergence identified in the cpDNA and ITS with genetic isolation and limited gene flow among regions suggested that the six populations separated well to two groups distributed upstream and downstream. The MaxEnt modeling results indicated that obvious unidirectional eastward migration via Yangtze River gorges watercourse mediated from Last Interglacial to Last Glacial Maximum were showed with the narrow scale distributions of six remnant populations and nine extirpated populations. The initial habitat fragmentation could be triggered by the accumulation of local habitat loss of the impoundment of the TGR during the Present period and might remain stable restoration with bidirectional diffusion in the Future. Divergences among M. laxiflora populations might have been induced by the drastic changes of the external environment and limited seed/pollen dispersal capacity, as the results of long-term ecological adaptability of summer flooding stress. The haplotypes of nuclear gene could be used for population's differentiation and germplasm protection. This identified gene flow and range dynamics have provided support for the gene-flow and geology hypothesis. It is also crucial for rescuing conservation to understand the impact of environmental dynamics on endangered organism evolution.

The use of predator tags to explain reversal movement patterns in Atlantic salmon smolts (Salmo salar L.).

Acoustic telemetry has seen a rapid increase in utility and sophistication in recent years and is now used extensively to assess the behavior and survival rates of many aquatic animals, including the Atlantic salmon. As part of the salmon's complex life cycle, salmon smolts are thought to make a unidirectional migration from fresh water to the sea, which is initiated by changes in their physiology. However, some tag movement patterns do not conform with this and can be difficult to explain, particularly if the tagged fish has been eaten by a predator. This study combines the use of predator tags with machine learning techniques to understand the fate of migrating salmon smolts and thereby improve estimates for migration success. Over 3 years between 2020 and 2022, 217 salmon smolts (including wild and hatchery-reared ranched fish) were acoustically tagged and released into an embayment on the west coast of Ireland. Some tagged smolts were observed to return from the estuary back into a saline lagoon through which they had already migrated. To distinguish between the movement of a salmon smolt and that of a predator, predator tags were deployed in migrating smolts in 2021 and 2022. The addition of a temperature sensor in 2022 enabled the determination of predator type causing the returning movement. A significant number of predator tags were triggered, and the patterns of movement associated with these triggered tags were then used with two types of machine learning algorithms (hierarchical cluster analysis and random forest) to identify and validate the behavior of smolts tagged without extra sensors. Both models produced the same outputs, grouping smolts tagged with predator tags with smolts tagged without the additional sensors but showing similar movements. A mammalian predator was identified as the cause of most reversal movement, and hatchery-reared ranched smolts were found to be more likely predated upon by this predator than wild smolts within the lake and the estuary. However, overall migration success estimates were similar for both wild and hatchery-reared ranched fish. This study highlights the value of predator tags as an essential tool in the overall validation of detection data.

Shape of scaffold controlling the direction of cell migration.

Cell migration plays an important role in the development and maintenance of multicellular organisms. Factors that induce cell migration and mechanisms controlling their expression are important for determining the mechanisms of factor-induced cell migration. Despite progress in the study of factor-induced cytotaxis, including chemotaxis and haptotaxis, precise control of the direction of cell migration over a wide area has not yet been achieved. Success in this area would update the cell migration assays, superior cell separation technologies, and artificial organs with high biocompatibility. The present study therefore sought to control the direction of cell migration over a wide area by adjusting the three-dimensional shape of the cell scaffold. The direction of cell migration was influenced by the shape of the cell scaffold, thereby optimizing cell adhesion and protrusion. Anisotropic arrangement of these three-dimensional shapes into a periodic structure induced unidirectional cell migration. Three factors were required for unidirectional cell migration: 1) the sizes of the anisotropic periodic structures had to be equal to or lower than the size of the spreading cells, 2) cell migration was restricted to a runway approximately the width of the cell, and 3) cells had to be prone to extension of long protrusions in one direction. Because the first two factors had been identified previously in studies of cell migration in one direction using two-dimensional shaped patterns, these three factors are likely important for the mechanism by which cell scaffold shapes regulate cell migration.

Exploring migration dynamics and housing markets in transition countries: Recommendations for city management and public health

This article investigates the multifaceted challenges resulting from concentrated and unidirectional migration to Bishkek, the capital of the Kyrgyz Republic. The study addresses the urgent need to rectify disparities in internal migration patterns and housing opportunities within Bishkek. By analyzing the intricate relationship between housing dynamics and migration inflows, the research underscores the critical importance of these dynamics for effective urban planning. A novel approach is proposed, advocating for the creation of a Bishkek agglomeration model aimed at fostering more balanced urban development and creating an accessible environment for public health. The primary objective of this study is to elucidate the correlation between the construction of new housing infrastructure and the overall state of housing deprivation across the country. The findings provide valuable insights and recommendations for administrative bodies responsible for managing migration and housing policies aimed at creating a healthy living environment.

Miocene Stromboid Gastropods (Superfamily Stromboidea Rafinesque, 1815) from the Dwarka Basin, Western India and their Paleobiogeographic Implications

Abstract Stromboid gastropods (Superfamily Stromboidea) are one of the most diverse groups of gastropods present in the Neogene marine successions of western India. In the present endeavor, we report eight stromboid species, of which four are described as new, from the early-middle Miocene marine successions of the Dwarka Basin, western India. The species are Conomurex indica n. sp., Persististrombus deperditus, Persististrombus sp., Dilatilabrum mahalonobisi n. sp., Tibia indica, Terebellum obtusum, Hemithersitea kanerus n. sp. and Hemithersitea nadharus n. sp. Paleobiogeographic distribution of most of the stromboid genera reported here reveals a unidirectional migration from the Tethys Region towards the Indo-Pacific Region during the Paleogene and Neogene. Conomurex originated in the western India and further radiated to the eastern Indian-Western Pacific localities with the onset of the Neogene. Persististrombus and Tibia show widespread distribution in the Mediterranean and gradually radiated towards the eastern Africa-western India localities during the Paleogene. However, from the middle Miocene onwards, these two genera further migrated towards the eastern Indian-Western Pacific localities and became more diverse. The remaining two genera, i.e., Dilatilabrum and Hemithersitea originated in the Mediterranean and later migrated to take refuge in western India during the late Paleogene, and ultimately succumbed to extinction during the middle Miocene.

Layer-By-Layer Assembly of Atomically Precise Alloy Nanoclusters Photosystems for Solar Water Oxidation.

Atomically precise metal nanoclusters (NCs) have garnered tremendous attention as light-harvesting antennas in heterogeneous photocatalysis due to unique atomic stacking mode, quantum confinement effect, and enriched active sites. However, metal NCs as photosensitizers suffer from extremely short carrier lifetime, poor photostability, and difficulty in carrier migration, which hinder the wide-spread utilization of metal NCs in solar energy conversion. To solve these problems, herein, Ag-doped glutathione (GSH)-capped gold NCs, i.e., alloy Au1- x Agx @GSH NCs and non-conjugated insulating polymer of poly(diallyl-dimethylammonium chloride) (PDDA) are utilized as the building blocks for layer-by-layer assembly of spatially multilayered alloy NCs/metal oxide (MO) photosystems. The alternately deposited ultrathin PDDA layer in-between Au1- x Agx @GSH NCs on the MO substrate functions as an efficient charge flow mediator to relay the directional photoelectron transfer over Au1- x Agx @GSH NCs, giving rise to the cascade charge transfer chain. This peculiar carrier migration mode endowed by exquisite interface configuration design significantly boosts the unidirectional electron migration from the Au1- x Agx @GSH NCs to the MO substrate, substantially improving the visible-light-driven photoelectrochemical water oxidation performances of MO/(PDDA-Au1- x Agx )n multilayer heterostructured photoanodes. The work will inspire the rational construction of alloy metal NCs-based photosystems for modulating spatially controllable charge transfer pathway for solar energy conversion.

Forced Emplacement

Abstract As intensifying floods and other climate extremes proliferate, narratives of unidirectional climate migration have become ubiquitous in media coverage and policy debates. This article reviews new scholarship that attends to an underreported dimension of climate change impact exposure. Emerging conversations in Indigenous climate justice research, mobility studies, and critical urban adaptation scholarship seek to understand why so many marginalized communities find themselves immobilized in the face of climate extremes. I argue that these scholars are building a concept of forced emplacement to politicize and historicize the uneven distribution of climate harms. Drawing on this scholarship and brief ethnographic sketches from my work in Peru and the Maldives, I follow forced emplacement across diverse case studies that root devastating immobilizations from flooding in local histories of colonial confinement, unevenly policed mobility, and varied efforts to control marginalized populations. I also illuminate how climate-exposed communities contest adaptation projects that reproduce their immobilization.

Engineering interfacial band hole extraction on chemical-vapor-deposited MoS2/CdS core-shell heterojunction photoanode: The junction thickness effects on photoelectrochemical performance

Heterojunction fabrication is a promising strategy that can greatly boost the charge carrier separation and improve the solar-to-hydrogen conversion efficiency of photoelectrochemical (PEC) cells. However, such technology still suffers from limited contact interfaces. In this study, the chemical vapor deposition (CVD) technique was for the first time used to construct the CdS/MoS2 heterojunction photoanode with a unique core-shell nanoarchitecture, in which a continuous crystalline MoS2 nanosheet layer was grown directly on one-dimensional (1D) oriented CdS nanorods (NRs) in a plane-to-plane stacking fashion. The optimization of junction thickness with adjustable MoS2 loading from mono to a few layers was achieved by experimental parameters variation. Systematic characterizations show that the MoS2 shell plays a dual role as an optical absorption booster for more photo-exciton generation and a surface passivator of trap states. Meanwhile, the formed heterojunction helps regulate the unidirectional charge migration for a significantly suppressed electron-hole recombination process, which synergistically contributes to higher quantum yield and efficiency. As a result, the optimized CdS/MoS2 heterojunction photoanode with 3-layered MoS2 wrapping exhibits the highest photocurrent density and photoconversion efficiency, over a two-fold increase, compared to those of pristine CdS and the previously reported CdS/MoS2 heterojunctions. Moreover, due to the rapid hole extraction from CdS and transferred surface oxidation sites, the present CdS/MoS2 heterostructure demonstrates better corrosion resistance and higher photostability. The present work is expected to provide a versatile platform for exploiting the CVD technique to develop other MoS2-based heterojunction photoelectrodes with extensive PEC applications.

Phylogeography and population genetic structure of the cardinal tetra (Paracheirodon axelrodi) in the Orinoco basin and Negro River (Amazon basin): evaluating connectivity and historical patterns of diversification.

The Neotropics contain one of the most diverse assemblages of freshwater fishes worldwide. Part of this diversity is shared between the Orinoco and Amazon basins. These basins have been separated for a long time due to the Vaupes Arch, rising between 10-11 Ma. Today, there is only one permanent connection between the Orinoco and Negro (Amazon) basins, known as the Casiquiare Canal. However, alternative corridors allowing fish dispersion between both basins have been proposed. The cardinal tetra (Paracheirodon axelrodi), the most important fish in the ornamental world market, is distributed in both basins. Here we investigated P. axelrodi phylogeography, population structure, and potential routes of migration and connectivity between the two basins. A total of 468 bp of the mitochondrial gene (COI), 555 bp of the nuclear gene fragment (MYH6), and eight microsatellite loci were analyzed. As a result, we found two major genetic clusters as the most likely scenario (K=2), but they were not discreetly distributed between basins. A gradient of genetic admixture was observed in Cucui and São Gabriel da Cachoeira, between the upper Negro River and the upper Orinoco. Samples from the middle-lower Negro River were highly structured. Cucui (Negro basin) was more similar to the Orinoco than to the rest of the Negro basin populations. However, substructure was also observed by the discriminant analysis, fixation indices and other hierarchichal structure analyses (K=3-6), showing three major geographic clusters: Orinoco, Cucui, and the remaining Negro basin. Unidirectional migration patterns were detected between basins: via Cucui toward Orinoco and via the remaining of the Negro basin toward Orinoco. Results from the Relaxed Random Walk analysis support a very recent origin of this species in the headwater Orinoco basin (Western Guiana Shield, at late Pleistocene) with a later rapid colonization of the remaining Orinoco basin and almost simultaneously the Negro River via Cucui, between 0.115 until about 0.001 Ma. Historical biogeography and population genetic patterns observed here for Cardinal tetra, seem to be better explained by river capture, physical, or ecological barriers than due to the geographic distance.

Single-Cell Analysis of Unidirectional Migration of Glioblastoma Cells Using a Fiber-Based Scaffold.

Glioblastoma (GBM) is a malignant incurable brain tumor in which immature neoplastic cells infiltrate brain tissue by spreading along nerve fibers. The aim of the study was to compare the migration abilities of glioma cells with those of other cancer cells and elucidate the migratory profiles underlying the differential migration of glioma cells using a fiber-based quantitative migration assay. Here, wound healing and transwell assays were used to assess cell mobility in four cell lines: U87-MG glioblastoma cells, MDA-MB-231 breast cancer cells, HCT116 colorectal cancer cells, and MKN45 gastric cancer cells. We also assessed cell mobility using a fiber model that mimics nerve fibers. Time-lapse video microscopy was used to observe cell migration and morphology. The cytoskeleton arrangement was assessed in the fiber model and compared with that in the conventional cell culture model. The conventional evaluation of cell migration ability revealed that the migration ability of breast cancer and glioblastoma cell lines was higher than that of colon cancer and gastric cancer cell lines. The fiber model confirmed that the glioblastoma cell line had a significantly higher migration ability than other cell lines. Tubulin levels were significantly higher in the glioblastoma cells than in other cell lines. In conclusion, the developed fiber-based culture model revealed the specific migratory profile of GBM cells during invasion.

Study on Barriers of Water Salt Transfers in Earthen Sites by Plastic-Coated Sand

Under the action of unidirectional water migration, museum soil sites generally encounter erosion through dry cracking, salt enrichment, etc. In this paper, the earthen site of the Terra Cotta Warriors Museum of the First Emperor of Qin was used as the research object, and the “hydrophobic” property of coated sand was proposed to prevent water migration and salt accumulation. Through the soil column experiment of water salt migration and the HYDRUS software numerical simulation, the water salt migration law of the soil in the heritage site under different conditions and the characteristics of water and salt resistance of plastic-coated sand were studied. The results showed that the salt damage on the earthen ruins was mainly due to the horizontal and vertical migration of water and salt in soil. After embedding the coated sand layer into the soil environment under the earthen site, the vertical and horizontal migration of water and salt in the soil can be completely prevented due to precipitation and groundwater. The coated sand protection technology and method proposed in this paper use materials similar to those of the earthen, and provide a feasible method for the protection of cultural relics in our country.

Urbanization of the population within the context of urban agglomeration development: a scientific perspective

This paper addresses the issues arising from active and unidirectional migration towards the capital city of Bishkek. The relevance of this work lies in resolving the problems associated with the disproportionate development of internal migration and the capacity of the city. Specifically, this paper examines problematic issues related to the development of residential areas for migrants and the necessity of forming small reference cities around Bishkek. Additionally, we study the housing and social problems that emerge due to the lack of a systematic approach to managing the natural process of urbanization. As a recommendation, we propose building a fundamentally new model of population urbanization in the form of the Bishkek agglomeration to address these impending issues. Therefore, the purpose of this study is to identify problems related to new migration residential buildings and justify the need to build supporting small towns around Bishkek. Finally, we provide recommendations for government and administrative services of the republic in regulating issues related to migration and housing policies.