Abstract Grain and rice characteristics are a description of the post-harvest quality of rice which is a consideration for farmers, traders, and consumers in choosing rice to be developed and consumed. This study aims to determine the yield, milling quality, rice quality and preference of 5 rice varieties in Indonesia, they were Inpari 32 HDB, Inpari 42 Agritan GSR, Inpari 45 Dirgahayu, Pamelen and Inpari IR Nutri Zinc. The study used a non-factorial Randomized Group Design, rice varieties as treatments, 5 replications. Rice was harvested using 2 methods, namely sickle (manual harvesting) and combine harvester. Determination of Milling Quality, rough rice was milled using a mini electric grain whitener LTJM-2008 then measured the percentage of hulled rice, unhulled rice, bran, and husk. Determination of rice quality, 25 grams of rice samples were sorted manually and measured the percentage of head rice, broken, groats and damaged then determined the grade of rice based on SNI 6128: 2015. The results showed that the rice yield manually and using a combine harvester was different from the potential yield. The milling quality and rice quality of each variety were significantly different (p=0.05%). Rice quality classification based on SNI 6128:2015 shows that Inpari 42 Agritan GSR and Inpari IR Nutri Zinc was included in Medium I, pamelen and Inpari 32 HDB were included in Medium II, and Inpari 45 Dirgahayu was under Medium III. Inpari 32 HDB was the rice variety with the highest preference.