Unfavorable Environment Research Articles

Tandem mass tag (TMT)-based quantitative proteomics analysis reveals the different responses of contrasting alfalfa varieties to drought stress

BackgroundDrought stress restricts the growth, distribution and productivity of alfalfa (Medicago sativa L.). In order to study the response differences of alfalfa cultivars to drought stress, we previously carried out physiological and molecular comparative analysis on two alfalfa varieties with contrasting drought resistance (relatively drought-tolerant Longdong and drought-sensitive Algonquin). However, the differences in proteomic factors of the two varieties in response to drought stress still need to be further studied. Therefore, TMT-based quantitative proteomic analysis was performed using leaf tissues of the two alfalfa cultivars to identify and uncover differentially abundant proteins (DAPs).ResultsIn total, 677 DAPs were identified in Algonquin and 277 in Longdong under drought stress. Subsequently, we conducted various bioinformatics analysis on these DAPs, including subcellular location, functional classification and biological pathway enrichment. The first two main COG functional categories of DAPs in both alfalfa varieties after drought stress were ‘Translation, ribosomal structure and biogenesis’ and ‘Posttranslational modification, protein turnover, chaperones’. According to KEGG database, the DAPs of the two alfalfa cultivars after drought treatment were differentially enriched in different biological pathways. The DAPs from Algonquin were enriched in ‘photosynthesis’ and ‘ribosome’. The pathways of ‘linoleic acid metabolism’, ‘protein processing in endoplasmic reticulum’ and ‘RNA transport’ in Longdong were significantly enriched. Finally, we found significant differences in DAP enrichment and expression patterns between Longdong and Algonquin in glycolysis/glycogenesis, TCA cycle, photosynthesis, protein biosynthesis, flavonoid and isoflavonoid biosynthesis, and plant-pathogen interaction pathway after drought treatment.ConclusionsThe differences of DAPs involved in various metabolic pathways may explain the differences in the resistance of the two varieties to drought stress. These DAPs can be used as candidate proteins for molecular breeding of alfalfa to cultivate new germplasm with more drought tolerance to adapt to unfavorable environments.

Neighbourhood environment and early menarche among adolescent girls of five countries

Introduction We aim to investigate the relationship between individuals’ perceptions of their neighbourhood environment and early menarche. Methods This was a retrospective cohort study of 7,486 girls of Ethiopia, India, South Korea, the United Kingdom (UK), and the United States (US), born in 1997–2011 was analysed. Early menarche was defined as being below the 10th to 20th percentiles in each cohort, considering the varying distributions across countries. Perceived neighbourhood environments were assessed based on the responses for neighbourhood pollution, safety, and recreational facilities. We calculated the relative risk (RR) of early menarche for unfavourable environment. Results The mean age at menarche was lowest in South Korea (10.6 years) and highest in Ethiopia (13.7 years). Unfavourable environment was associated with higher risk of early menarche overall (RR = 1.34, 95% confidence interval [CI]:1.09–1.65) and each country (3.03, 95% CI: 1.15–7.96 in Ethiopia; 1.99, 95% CI: 0.97–4.10 in India, 1.23, 95% CI: 0.67–2.27 in Korea; 1.26, 95% CI: 0.96–1.64 in the UK). Specifically, pollution (1.29, 95% CI: 1.03–1.62) and low safety (1.19, 95% CI: 1.60–1.88) were associated with early menarche. Conclusions Our finding highlights the potential role of perceived neighbourhood environment in the timing of puberty.

Exploring the “Cascade Effect” of AEBSF on Reduced Glioblastoma Proliferation

Glioblastomas (GBMs) are one of the deadliest cancers, and yet still lack treatment. In this study, serine protease inhibitor AEBSF is shown to decrease GBM vi-ability and proliferation through a series of intertwined steps. By decreasing the expres-sion of ATP6V1A--the first step of the “cascade effect”--AEBSF creates an unfavorable environment for protease activity. Such “unfavorability” is proven by a decline in expres-sion of proteases MMP9 and ELANE upon GBM treatment with AEBSF. Increasingly higher concentrations of AEBSF are shown to decrease the percentage of cell viability, proliferation, and metastasis. This is explained through biological process enrichment analyses, which indicate that MMP9 and ELANE play a role in the disassembly of the extracellular protein network. This disassembly of the extracellular environment enables tumorigenic spread and viability. The results of the viability and proliferation assays, in combination with those of the indirect ELISAs, enrichment analyses, and scratch assays, underscore an intricate and profound sequence of events that constitute the “cascade effect”.

Development Resources of the Border Regions of the Russian Federation

The Russian Federation borders with 18 foreign countries. Seven of them are NATO members as of March 2024. The state border of Russia passes through the territory of almost half of the constituent entities of the Russian Federation. The shelling of the territory of the Russian Federation by the armed forces of Ukraine radically changes the situation in the sphere of not only of military, but also of socio-economic security, primarily in the border regions of Russia. The issue of their protection, elimination of weaknesses and organization of society’s life in a new, unstable, largely unfavorable external environment is especially acute. The formation of resources necessary for solving these issues largely depends on the current level of economic development of the country and its border regions. The author’s purpose was to illustrate some aspects of the economic situation in the border regions of the Russian Federation, given the current circumstances where Russia’s adversaries are attempting to create areas of tension near the Russian borders, exemplified by Ukraine, Poland, and the Baltic States. To achieve this goal, the author solved several tasks. They are: selecting the subjects of the Russian Federation to be studied, determining the set of statistical indicators to be analyzed, and determining the length of the dynamic range for each indicator. We also constructed the dynamics of change for these selected indicators. The results of the author’s analysis of seven statistical indicators that reflect the socio-economic situation in 36 selected border regions of the Russian Federation are presented below. The analyzed statistical data are taken for the period from 1995 to 2021. The economic and statistical research was carried out on the basis of data from the Federal State Statistics Service of the Russian Federation, most of which are published in statistical collections “Regions of Russia. Socio-economic Indicators”. The article shows that a number of the studied parameters indicate the need to strengthen the economic power of the Russian border regions in modern and possible future conditions. The obtained results can be useful for legislative and executive authorities of the Russian Federation and the subjects of the Russian Federation in the development and implementation of the development policy of regions that have a particularly important strategic importance.

The role of alternative autophagy in cell viability and response to paclitaxel treatment in v-Ha-ras-transformed NIH 3T3 cells

In confluent v-Ha-ras-transformed NIH 3T3 fibroblasts (Ras-NIH 3T3), LC3 downregulation may precede a decrease in canonical autophagy, thus contributing to cell survival. Herein, we aimed to investigate the role of alternative autophagy in the viability of long-term cultures of Ras-NIH 3T3 cells and their parental NIH 3T3 cells. As cell confluence increased with the culture period, the level of alternative autophagy, as assessed through Lamp2-Rab9 co-localization, gradually decreased in both cell lines. However, Ras-NIH 3T3 cells maintained higher levels of alternative autophagy than the parental cells did. Rab9 knockdown minimally affected NIH 3T3 cells while drastically reducing the viability of Ras-NIH 3T3 cells, which suggested that alternative autophagy plays a critical role in Ras-NIH 3T3 cells. In contrast, reactive oxygen species (ROS) production in Ras-NIH 3T3 cells was higher than that in NIH 3T3 cells during long-term culture. Moreover, NIH 3T3 cells exhibited a continual decrease in mitochondrial mass, whereas Ras-NIH 3T3 cells maintained high mitochondrial mass. Immunofluorescence analysis of mitochondrial membrane marker proteins and mitochondrial membrane potential (MMP) also demonstrated a temporal pattern of changes similar to those of mitochondrial mass. This finding could be attributed to the relatively higher level of alternative autophagy in Ras-NIH 3T3 cells facilitating the removal of damaged mitochondria. Paclitaxel treatment in Ras-NIH 3T3 cells induced an increase in canonical autophagy rates along with suppression of alternative autophagy. Ras-NIH 3T3 cells showed high sensitivity to paclitaxel at the early stage of culture, but as cell confluence increased, resistance to paclitaxel increased, showing a similar level of cell viability to the vehicle control group. The study findings suggest that alternative autophagy is more important than canonical autophagy for maintaining cell survival in response to an unfavorable environment, such as during high cell confluence and exposure to anticancer agents.

An unfavorable indoor environment as mediator between fuel poverty and health: an exploration

Abstract Aim The objective of this study is to gain insight into the extent to which an unfavorable indoor environment mediates the association between fuel poverty and health. Method Data from the 2022 Dutch Health Survey were enriched with some registration data from the 2020 Monitor Fuel Poverty, resulting in a study population of 16,210 adults. Using Structural Equation Modeling in R, the mediation effect of an unfavorable indoor environment on the relationship between fuel poverty and various health outcomes was examined. An unfavorable indoor environment was defined as moisture, mold, and/or inadequate ventilation, as indicators of housing quality. Analyses were adjusted for various demographic factors: age, gender, property ownership (tenants or not), level of education, household with or with no children, and level of urbanity of the residence. Results Fuel poverty is negatively associated with the absence of mental health issues and with social capital and positively associated with the risk of anxiety or depression, a negative self-rated health, physical limitations in daily life, loneliness, and stress. These associations are partially mediated by an unfavorable indoor environment (mediation proportion: 5.5–10.8%). In a subgroup analysis of tenants of housing corporations, the mediation proportion ranges from 8.3 to 20.1%. Conclusion An unfavorable housing quality with moisture, mold, and/or inadequate ventilation is a mediating factor in the relationship between fuel poverty and health. Addressing the health effects of fuel poverty requires a comprehensive and structural approach, of which the indoor environment is an integral part. Connecting fuel poverty, housing quality, and health is necessary in both research and policy addressing fuel poverty.

Critique of Vijay Tendulkar Plays with a Special Reference on Kanyadaan and Sakharam Binder

This paper is a sum up of Vijay Tendulkar’s works and gives us a gist of his works. This project will tell us about the complicated relationship between their society and mental distortion. It explores the role of masculinity in our society. This paper will briefly discuss the portrayal of women characters in Vijay Tendulkar’s play. The family, personal, political, and social environments that Vijay Tendulkar’s plays depict lead to a great deal of violence against the characters- physically, sexually, psychologically, and verbally. Tendulkar’s play represents middle-class life, and because of his deeply held beliefs, the men in his plays frequently treat their female counterparts brutally. The paper’s conclusions discuss how the female protagonists overcome their unfavorable environment to become strong, and, independent people.

CORRECTION OF HEALTH DEVIATIONS CAUSED BY UNFAVORABLE ENVIRONMENTAL CONDITIONS USING NON-PHARMACOLOGICAL MEANS

Lack of atmospheric oxygen (exogenous hypoxia) in residents of high mountain areas leads to functional disturbances of blood circulation, blood composition, activity of tissue respiration enzymes and resulting other biochemical abnormalities, causing a number of diseases. And the open mines operating in these regions create an unfavorable ecological environment, contributing to the deepening of functional disorders of the body among the population. Adolescents in this region are also at risk for the development of pathological processes. These risks can be reduced, and diseases can be prevented using non-drug means, using herbs with antihypoxic effects and special breathing exercises.

Higher anxiety level and associated work-related factors of delivery workers in South Korea: from the 6th Korean working conditions survey.

This study investigates anxiety risk and work-related factors among platform workers. The recent growth in the platform industry is a worldwide trend, with delivery workers in Korea representing typical platform workers. This cross-sectional study used the 6th Korean Working Conditions Survey to assess anxiety risk among 532 delivery workers compared to general employees. It identified associations between work-related factors and anxiety, including job demands and autonomy, legal protection, and emotional labor. Delivery workers exhibited significantly higher anxiety (odds ratio [OR] = 1.67 95% confidence interval [CI] = 1.23-2.28) and work-related anxiety (OR = 2.17 95% CI = 1.48-3.18) risk than the general workforce, and a significantly higher risk of having unfavorable work environment factors. Their anxiety risk was significantly associated with work-related factors, such as long shifts, quick return, time pressure, job stress, absence of union, work-family conflict, and emotional labor. This study identified high levels of anxiety among Korean delivery workers and associated occupational factors. It highlights the importance for industry and government interventions to enhance mental health support, mitigate poor employment conditions, and ensure legal safeguards.

Quantification of morpho-hemodynamic changes in unruptured intracranial aneurysms with irregular pulsation during the cardiac cycle using 4D-CTA.

Previous studies predicting the rupture risk of intracranial aneurysms (IAs) have predominantly utilized static imaging data, overlooking the dynamic blood flow and biomechanical properties of the aneurysm wall. Irregular pulsation detected by 4D-CTA is a potential predictor of aneurysm rupture, albeit with uncertain clinical significance. This study aimed to analyze the changes in morpho-hemodynamic characteristics of IAs during the cardiac cycle to elucidate the dynamic changes and the associated hemodynamic mechanisms. A retrospective review was conducted on the 4D-CTA data of IA patients between January 2017 and September 2019. R-R intervals were segmented into 20-time phases, reconstructing 20 CT datasets to identify irregular pulsation and extract 3D aneurysm models. Computational fluid dynamics (CFD) simulations analyzed hemodynamic parameters such as oscillatory shear index (OSI) and relative residence time (RRT). Changes in morpho-hemodynamic characteristics were quantified in terms of the absolute change (parameter*) and relative change rate (parameter%). Rupture risk was assessed using the rupture resemblance model (RRS). Eleven UIAs from 10 patients were finally included, with five aneurysms showing irregular pulsation (45.45%). No significant differences in morpho-hemodynamic characteristics were observed between aneurysms with or without irregular pulsation. More remarkable changes in aneurysm size (size*: 0.59 ± 0.14 mm vs. 0.32 ± 0.12 mm, p = 0.010; size%: 10.49% ± 1.43% vs. 3.95% ± 1.79%, p < 0.001), volume (volume%: 13.72% vs. 6.39%, p = 0.009), OSI (OSI*: 0.02 ± 0.01 vs. 0.004 ± 0.005, p = 0.004; OSI%: 200% vs. 12.50%, p = 0.004) and RRT (RRT%: 97.14% vs. 43.95, p = 0.052) over the cardiac cycle were significantly linked to irregular pulsation. Aneurysms with irregular pulsation demonstrated a more unfavorable hemodynamic environment during the cardiac cycle, irrespective of the predicted rupture risk. Furthermore, irregular pulsation at the aneurysm dome exhibited higher hemodynamic instability than at the sidewall. Irregular pulsation may indicate hemodynamic instability within the aneurysm, leading to an increased rupture risk in the area where irregular pulsation occurs. This proof-of-concept study could enhance understanding of dynamic changes in UIAs during the cardiac cycle and the underlying hemodynamic mechanisms.

Effect of Temperature on Some Biological Indicators of Cydia pomonella Linnaeus, 1758

In 2022-2023 The influence of temperature on the development and reproduction of the codling moth butterfly was studied. Experiments have shown that the optimal temperature regime for mass reproduction of the codling moth in laboratory conditions is within 20-25°C. At low temperatures (15°C), butterflies live 34-35 days, and some individuals can live up to 45-50 days. This shows that these characteristics of butterflies allow them to remain viable in an unfavorable environment, although their reproduction is reduced. The experiments carried out make it possible to obtain the necessary temperature parameters for the development of fruit-eating apple trees.

Contrasting patterns of spatial genetic structure in endangered southern damselfly (Coenagrion mercuriale) populations facing habitat fragmentation and urbanisation

AbstractAimHuman‐induced environmental changes result in habitat loss and fragmentation, impacting wildlife population genetic structure and evolution. Urbanised and geographically peripheral areas often represent unfavourable environments, reducing connectivity among populations and causing higher population genetic differentiation and lower intra‐population genetic diversity. We examined how geographic peripherality and anthropogenic pressures affect genetic diversity and genetic differentiation in the protected southern damselfly (Coenagrion mercuriale, Odonata), which has low dispersal capabilities and specific habitat requirements and whose populations are declining.LocationWe studied two areas: one in semi‐natural habitats at the periphery of the species geographic range (northern France) and the other more central to the species' range, in an urbanised area surrounding the city of Strasbourg (Alsace, eastern France).MethodsWe genotyped 2743 individuals from 128 populations using 11 microsatellite loci. We analysed the spatial distribution of neutral genetic diversity (allelic richness, heterozygosity, levels of inbreeding and genetic relatedness), the extent of genetic differentiation and population affiliations (sPCAs) within the two areas. We also examined fine‐scale patterns of gene flow in the urbanised area of Alsace by investigating patterns of isolation by distance and estimating effective migration surfaces (EEMS) method.ResultsNorthern peripheral populations showed lower levels of genetic diversity and higher levels of genetic differentiation than central Alsacian populations. Although located in anthropised habitats, geographically central Alsacian populations showed high levels of gene flow, with dispersal events mainly occurring overland and not restricted to watercourses. However, the highly urbanised city of Strasbourg negatively impacted nearby populations by reducing levels of genetic diversity and increasing population genetic differentiation.Main ConclusionsThese results showed the need for management action by restoring breeding sites and creating migratory corridors for peripheral southern damselfly populations. However, our results also highlighted the resilience of southern damselfly in central range populations facing strong urbanisation pressures.

Multiple factors trigger the formation and resuscitation of the VBNC state in alcohol-producing Klebsiella pneumoniae.

Klebsiella pneumoniae can enter a viable but nonculturable (VBNC) state to survive in unfavorable environments. Our research found that high-, medium-, and low-alcohol-producing K. pneumoniae strains are associated with nonalcoholic fatty liver disease. However, the presence of the three Kpn strains has not been reported in the VBNC state or during resuscitation. In this study, the effects of different strains, salt concentrations, oxygen concentrations, temperatures, and nutrients in K. pneumoniae VBNC state were evaluated. The results showed that high-alcohol-producing K. pneumoniae induced a slower VBNC state than medium-alcohol-producing K. pneumoniae, and low-alcohol-producing K. pneumoniae. A high-salt concentration and micro-oxygen environment accelerated the loss of culturability. Simultaneously, both real-time quantitative PCR and droplet digital PCR were developed to compare the quantitative comparison of three Kpn strain VBNC states by counting single-copy gene numbers. At 22°C or 37°C, the number of culturable cells decreased significantly from about 108 to 105-106 CFU/mL. In addition, imipenem, ciprofloxacin, polymyxin, and phiW14 inhibited cell resuscitation but could not kill VBNC-state cells. These results revealed that the different environments evaluated play different roles in the VBNC induction process, and new effective strategies for eliminating VBNC-state cells need to be further studied. These findings provide a better understanding of VBNC-state occurrence, maintenance, detection, and absolute quantification, as well as metabolic studies of resuscitation resistance and ethanol production.IMPORTANCEBacteria may enter VBNC state under different harsh environments. Pathogenic VBNC bacteria cells in clinical and environmental samples pose a potential threat to public health because cells cannot be found by routine culture. The alcohol-producing Kpn VBNC state was not reported, and the influencing factors were unknown. The formation and recovery of VBNC state is a complete bacterial escape process. We evaluated the influence of multiple induction conditions on the formation of VBNC state and recovery from antibiotic and bacteriophage inhibition, and established a sensitive molecular method to enumerate the VBNC cells single-copy gene. The method can improve the sensitivity of pathogen detection in clinical, food, and environmental contamination monitoring, and outbreak warning. The study of the formation and recovery of VBNC-state cells under different stress environments will also promote the microbiological research on the development, adaptation, and resuscitation in VBNC-state ecology.

Cell death in bryophytes: emerging models to study core regulatory modules and conserved pathways.

This review summarizes recent progress in our current understanding of the mechanisms underlying the cell death pathways in bryophytes, focusing on conserved pathways and particularities in comparison to angiosperms. Regulated cell death (RCD) plays key roles during essential processes along the plant life cycle. It is part of specific developmental programmes and maintains homeostasis of the organism in response to unfavourable environments. Bryophytes could provide valuable models to study developmental RCD processes as well as those triggered by biotic and abiotic stresses. Some pathways analogous to those present in angiosperms occur in the gametophytic haploid generation of bryophytes, allowing direct genetic studies. In this review, we focus on such RCD programmes, identifying core conserved mechanisms and raising new key questions to analyse RCD from an evolutionary perspective.

PpGpp is a dual-role regulator involved in balancing iron absorption and prodiginine biosynthesis in Pseudoalteromonas.

Iron is an essential element for microbial survival and secondary metabolism. However, excess iron availability and overloaded secondary metabolites can hinder microbial growth and survival. Microorganisms must tightly control iron homeostasis and secondary metabolism. Our previous studies have found that the stringent starvation protein A (SspA) positively regulates prodiginine biosynthesis by activating iron uptake in Pseudoalteromonas sp. strain R3. It is believed that the interaction between SspA and the small nucleotide ppGpp is important for iron to exert regulation functions. However, the roles of ppGpp in iron absorption and prodiginine biosynthesis, and the underlying relationship between ppGpp and SspA in strain R3 remain unclear. In this study, we found that ppGpp accumulation in strain R3 could be induced by limiting iron. In addition, ppGpp not only positively regulated iron uptake and prodiginine biosynthesis via increasing the SspA level but also directly repressed iron uptake and prodiginine biosynthesis independent of SspA, highlighting the finding that ppGpp can stabilize both iron levels and prodiginine production. Notably, the abolishment of ppGpp significantly increased prodiginine production, thus providing a theoretical basis for manipulating prodiginine production in the future. This dynamic ppGpp-mediated interaction between iron uptake and prodiginine biosynthesis has significant implications for understanding the roles of nutrient uptake and secondary metabolism for the survival of bacteria in unfavorable environments.

Nexus between Despotic Leadership, Faculty Performance, and Faculty Behavior: A Case of a Pakistani Public University

The aim of this study was to examine the effects of despotic leadership on the output of university faculty members. The study was conducted by using a mixed methods technique in accordance with the pragmatism paradigm. The population of the study was the 74 permanent faculty members of the one Public Sector University's two campuses located in Lahore, Pakistan. A semi-structured interview protocol was used to interview 15 faculty members through a convenient sampling technique. Thematic analysis was used to examine the qualitative data, while descriptive analysis percentage (%) and frequency(f) were used to examine the quantitative data. The results demonstrate that faculty members may become uncomfortable and agitated due to the hostile behavior of the HODs, which could lead to a negative work environment and a decreased desire to share ideas. The unfavorable work environment and decreased job satisfaction are caused by the bad behavior of HODs. The HODs' menacing actions have a clear detrimental impact on staff members' performance, foster a hostile work atmosphere, and jeopardize faculty members' mental health. Based on the research findings, it is recommended that HODs should behave civilly toward faculty members. The HODs could also receive leadership training from the experts.

L’Assemblée provinciale et l’ingouvernabilité de la Province du Sud-Kivu : de la désillusion au nouveau départ

The 2018-2023 legislature started with an autosubliminal show in South Kivu, a province of Congo-Kinshasa. The spectacle led to an unfavourable environment to significant impact work production on the provincial governance. Members of provincial Assembly, mainly those who regularly fell into disputes, lost opportunity to emerge as political or institutional actors with a development-axed target. Thus, this paper aimed at analysing these behaviours and practices to find out their effects on the governance in province. This study is then a functioning conceptualization of the South-Kivu provincial Assembly in interaction with the provincial government management for the progress of the province. It is based on quality features. The south-kivucentrist pathology conceptualization is the main innovant point of this study. It has widely explained the real living situation in South-Kivu during the 2018-2023 legislature with its consequences on the socioeconomic sector of this part of the country. Our results have shown that no governance that promotes socioeconomical area can emerge from that pathological situation. The obtained results have given a new explanation to the power or governance phenomenon of South-Kivu. They include several dimensions in the point understanding by enlighting irrational digressions and deviant behaviour before some cure has been suggested. Keywords: Legislature, ungovernability, South-Kivu, south-kivucentrist pathology, stupidity.

Interaction of marketing and logistics activities of enterprises in the context of the challenges of the modern business environment of Ukraine

In the article, the directions of implementation of marketing and logistics activities in relation to increasing the control of the enterprise over the subjects and conditions of its external microenvironment were determined. Marketing was presented as a system of information interaction of the enterprise with the external environment, and logistics was presented as a system of establishing business relations with the external environment by constructing logistics chains. For both types of activity, the impact on the external microenvironment comes down to stimulating the desired behavior of subjects with whom the enterprise interacts with the help of informational, organizational and economic levers. The content of logistical support of the enterprise’s marketing activities based on the "7Ps" concept was detailed. It is shown that the support involves, among other things, the use of logistics services resources to perform specific marketing tasks. The content of marketing and logistics measures aimed at ensuring the sustainability of business in an unfavorable business environment in both cases is reduced to the formation of alternative options for resource provision of the corresponding type of activity, optimization of costs and diversification of goods/markets. The study was conducted of the dynamics of changes in the share of relevant types of economic activity in the total volume of products sold, the profitability of operational activities, assessment of changes in the business situation in the field of services, the volume of sold innovative products, costs for innovations. The results show the sufficient potential of the relevant components of the market infrastructure in terms of supporting domestic business entities, and indirectly indicate the sufficient potential of marketing and logistics activities of Ukrainian enterprises in terms of ensuring economic activity in the conditions of an unfavorable business environment.

The Burn Out of Health Professional: Case of the Tominian Health District

The health of the population is one of the priority sectors of the Malian state authorities. The implementation at the operational level of socio-health activities by health professionals remains a challenge given working conditions and societal realities. This situation in an unfavorable environment exposes these professionals to the development of burnout. Observation: It was a health professional, 48 years old, a civil servant for 10 years in the district, having consulted for insomnia, osteoarticular pain and anorexia. Mood disorders, nervousness, palpitations, physical asthenia persisting for 7 months had been observed. Several consultations and assessments carried out without success. The persistence of the symptoms and the negativity of the assessments made it possible to raise the hypothesis of a professional burnout syndrome. A treatment based on rest over 1 month combined with anxiolytics for 15 days allowed the symptoms to improve. Follow-up for 11 months was unremarkable. Conclusion: The workload, the insufficiency in the organization, the planning of activities and sometimes the failure of the patient to fulfill their duties in our structures are determining factors in the occurrence of burnout among health professionals. Sufficient recruitment of professionals at the operational level, health education and the implementation of an adequate work plan could reduce this risk.

THE RELATIONSHIP OF PERSONAL HYGIENE WITH THE INSIDENT OF SCABIES IN ADOLESCENTS

Scabies is an infectious disease that is at risk for people with weak immune systems and living in unfavorable environments. The factor that influences the incidence of scabies is personal hygiene. The aim of this research was to determine the relationship between personal hygiene and the incidence of scabies in adolescents at the Al Istiqomah integrated Islamic boarding school in the working area of ​​the UPTD Gunungguruh Community Health Center, Sukabumi Regency. Scabies is a skin disease caused by the Sarcoptes Scabiei hominis variant. Personal hygiene is an action in maintaining individual cleanliness and health with the aim of achieving physical and psychological well-being. Type of correlational research with a Cross Sectional approach. The population in this study were teenagers at the Al Istiqomah integrated Islamic boarding school with a sample of 126 respondents. The sampling technique was simple random sampling. Data collection method through questionnaires. Data analysis used the Chi-Square Test. The results showed that the majority had good personal hygiene (76.2%) and did not experience scabies (57.9%). There is a relationship between personal hygiene and the incidence of scabies with a p-value of 0.000. The conclusion is that there is a relationship between personal hygiene and the incidence of scabies in teenagers. The efforts made by the Gunungguruh Community Health Center include providing education to all students using easy-to-understand media that can be carried out in everyday life.