Unexpected Frequency Research Articles

Gravity-based microfiltration reveals unexpected prevalence of circulating tumor cell clusters in ovarian and colorectal cancer

BackgroundCirculating tumor cells (CTCs) are rare (a few cells per milliliter of blood) and mostly isolated as single-cell CTCs (scCTCs). CTC clusters (cCTCs), even rarer, are of growing interest, notably because of their higher metastatic potential, but very difficult to isolate.MethodWe introduce gravity-based microfiltration (GµF) for facile isolation of cCTCs using in-house fabricated microfilters and 3D printed cartridges. Optimal flow rate and pore size for cCTC isolation are determined by GµF of cultured ovarian single cells and cell clusters spiked in healthy blood. We perform GµF of blood from orthotopic ovarian cancer mouse models and characterize the morphological features of scCTCs and cCTCs, and the expression of molecular markers for aggressiveness. Finally, we analyze blood from 17 epithelial ovarian cancer patients with either localized or metastatic disease, and from 13 colorectal cancer liver metastasis patients.ResultsHere, we show that GµF optimized for cell cluster isolation captures cCTCs from blood while minimizing unwanted cluster disaggregation, with ~85% capture efficiency. We detect cCTCs in every patient, with between 2–100+ cells. We find cCTCs represent between 5–30% of all CTC capture events, and 10-80% of the CTCs are clustered; remarkably, in 10 patients, most CTCs are circulating not as scCTCs, but as cCTCs.ConclusionsGµF uncovers the unexpected prevalence and frequency of cCTCs including sometimes very large ones in epithelial ovarian cancer patients, and motivates additional studies to uncover their properties and role in disease progression.

A hybrid transformer masked time-domain denoising network for vibration signals

Abstract Health-condition-sensitive vibration information is prone to be swamped by widespread noise. Denoising is always indispensable, but existing methods still lack adaptability. Therefore, a novel intelligent denoising framework called a hybrid transformer masked time-domain denoising network (HTMTDN) is proposed. First, a dense dilation convolution block and a hybrid transformer are constructed to deal with fault impulse scale variations and unexpected noise frequency bands respectively, which greatly improves the adaptive denoising capability and relieves tough denoising parameter tuning. Further, interpretable time- and frequency-domain joint constraints are constructed to enhance the network’s optimization ability under strong noise. Finally, a novel strategy called overlapping reconstruction is introduced to recover 1D signals from 2D signal segments. Extensive experiments based on two bearing fault datasets with variable loads and rotation speeds confirm the remarkable performance of the HTMTDN under low signalnoise ratios, and present good adaptability in 15 health conditions without separate hyperparameter tuning, which shows promise for real-world applications.

Unexpected frequency of horizontal oscillations of magnetic structures in the solar photosphere

It is well known that the dominant frequency of oscillations in the solar photosphere is ≈3 mHz, which is the result of global resonant modes pertaining to the whole stellar structure. However, analyses of the horizontal motions of nearly 1 million photospheric magnetic elements spanning the entirety of solar cycle 24 have revealed an unexpected dominant frequency, ≈5 mHz, a frequency typically synonymous with the chromosphere. Given the distinctly different physical properties of the magnetic elements examined in our statistical sample, when compared to largely quiescent solar plasma where ≈3 mHz frequencies are omnipresent, we argue that the dominant ≈5 mHz frequency is not caused by the buffeting of magnetic elements, but instead is due to the nature of the underlying oscillatory driver itself. This novel result was obtained by exploiting the unmatched spatial and temporal coverage of magnetograms acquired by the Helioseismic and Magnetic Imager (HMI) on board NASA’s Solar Dynamics Observatory (SDO). Our findings provide a timely avenue for future exploration of the magnetic connectivity between sub-photospheric, photospheric, and chromospheric layers of the Sun’s dynamic atmosphere.

Non-smooth dynamics of impacting viscoelastic pipes conveying pulsatile fluid

This paper aims to investigate the stability and nonlinear dynamics of an initially curved viscoelastic clamped–clamped pipe conveying pulsatile fluid, with special attention to the non-smooth dynamics of this gyroscopic system under the influence of initial curvature and gravity. In this study, the impacting contact is modeled as a trilinear repulsive force. Based on the Euler–Bernoulli beam theory, the nonlinear governing equations, which take into account geometric, curvature, and damping nonlinearities, are developed using Hamilton’s principle. The continuous model is discretized using the Galerkin method and then solved using the arc-length technique and a time integration method. A linear analysis of the natural frequency and complex mode shape shows the unexpected frequency intersection and modal asymmetry. The qualitative changes in the nonlinear dynamics of the impacting pipe in both sub- and super-critical regions are examined and presented in the form of bifurcation diagrams, frequency–amplitude curves, force–amplitude curves, time histories, and phase portraits. Numerical results show that some complex dynamical behaviors can occur in different impact constraint cases, including a grazing bifurcation, sticking, period doubling, and chaos at the impact location, may occur. The initial curvature and gravity result in different behaviors, such as the asymmetry change of the equilibrium configuration and the occurrence of the flutter instability. The current analysis is significant to understand the non-smooth dynamic mechanisms and also provide design and technology guidance for the pipe system with motion-limiting constraints.

Control of quality factor of atomic force microscopy cantilever by cavity optomechanical effect

The effective quality factor of the cantilever plays a fundamental role in dynamic mode atomic force microscopy. Here we present a technique to modify the quality factor of an atomic force microscopy cantilever within a Fabry–Perot optical interferometer. The experimental setup uses two separate laser sources to detect and excite the oscillation of the cantilever. While the intensity modulation of the excitation laser drives the oscillation of the cantilever, the average intensity can be used to modify the quality factor via optomechanical force without changing the fiber-cantilever cavity length. The technique enables users to optimize the quality factor for different types of measurements without influencing the deflection measurement sensitivity. An unexpected frequency shift was observed and modelled as temperature dependence of the cantilever’s Young’s modulus, which was validated using finite element simulation. The model was used to compensate for the thermal frequency shift. The simulation provided relations between optical power, temperature, and frequency shift.

Unexpected high frequency of Krabbe disease detected by newborn screening in Italy

Unexpected high frequency of Krabbe disease detected by newborn screening in Italy

Comparison of Unexpected Antibody Frequency in Korea by Department: Focusing on Obstetrics and Infertility Centers

Comparison of Unexpected Antibody Frequency in Korea by Department: Focusing on Obstetrics and Infertility Centers

Magnetstein: An Open-Source Tool for Quantitative NMR Mixture Analysis Robust to Low Resolution, Distorted Lineshapes, and Peak Shifts.

1H NMR spectroscopy is a powerful tool for analyzing mixtures including determining the concentrations of individual components. When signals from multiple compounds overlap, this task requires computational solutions. They are typically based on peak-picking and the comparison of obtained peak lists with libraries of individual components. This can fail if peaks are not sufficiently resolved or when peak positions differ between the library and the mixture. In this paper, we present Magnetstein, a quantification algorithm rooted in the optimal transport theory that makes it robust to unexpected frequency shifts and overlapping signals. Thanks to this, Magnetstein can quantitatively analyze difficult spectra with the estimation trueness an order of magnitude higher than that of commercial tools. Furthermore, the method is easier to use than other approaches, having only two parameters with default values applicable to a broad range of experiments and requiring little to no preprocessing of the spectra.

Critical zone identification framework for bulk electric system security assessment

Climate change introduces a new set of vulnerabilities. It might cause unexpected voltage and frequency violation problems that can damage the existing power system configurations. Identifying the zones containing more critical violations in a bulk system under different climatic conditions and contingencies is crucial to securing the power system operation and infrastructure safety. This paper presents an advanced critical zone identification framework that can retrieve information from extensive data yielded by dynamic contingency analysis results from a large interconnected power grid. A machine learning (ML) based clustering approach is applied to identify the critical zone under hundreds of scenarios. The developed framework is tested using the 2028 western electricity coordinating council (WECC) system, and the detailed results are discussed in this paper.

Phylogenomics illuminates the phylogeny of flower weevils (Curculioninae) and reveals ten independent origins of brood-site pollination mutualism in true weevils.

Weevils are an unusually species-rich group of phytophagous insects for which there is increasing evidence of frequent involvement in brood-site pollination. This study examines phylogenetic patterns in the emergence of brood-site pollination mutualism among one of the most speciose beetle groups, the flower weevils (subfamily Curculioninae). We analysed a novel phylogenomic dataset consisting of 214 nuclear loci for 202 weevil species, with a sampling that mainly includes flower weevils as well as representatives of all major lineages of true weevils (Curculionidae). Our phylogenomic analyses establish a uniquely comprehensive phylogenetic framework for Curculioninae and provide new insights into the relationships among lineages of true weevils. Based on this phylogeny, statistical reconstruction of ancestral character states revealed at least 10 independent origins of brood-site pollination in higher weevils through transitions from ancestral associations with reproductive structures in the larval stage. Broadly, our results illuminate the unexpected frequency with which true weevils-typically specialized phytophages and hence antagonists of plants-have evolved mutualistic interactions of ecological significance that are key to both weevil and plant evolutionary fitness and thus a component of their deeply intertwined macroevolutionary success.

Acute megakaryoblastic leukaemia shows high frequency of chromosome 1q aberrations and dismal outcome.

Acute megakaryoblastic leukaemia (AMKL) is associated with poor prognosis. Limited information is available on its cytogenetics, molecular genetics and clinical outcome. We performed genetic analyses, evaluated prognostic factors and the value of allogeneic haematopoietic stem cell transplantation (allo-HSCT) in a homogenous adult AMKL patient cohort. We retrospectively analysed 38 adult patients with AMKL (median age: 58 years, range: 21-80). Most received intensive treatment in AML Cooperative Group (AMLCG) trials between 2001 and 2016. Cytogenetic data showed an accumulation of adverse risk markers according to ELN 2017 and an unexpected high frequency of structural aberrations on chromosome arm 1q (33%). Most frequently, mutations occurred in TET2 (23%), TP53 (23%), JAK2 (19%), PTPN11 (19%) and RUNX1 (15%). Complete remission rate in 33 patients receiving intensive chemotherapy was 33% and median overall survival (OS) was 33 weeks (95% CI: 21-45). Patients undergoing allo-HSCT (n = 14) had a superior median OS (68 weeks; 95% CI: 11-126) and relapse-free survival (RFS) of 27 weeks (95% CI: 4-50), although cumulative incidence of relapse after allo-HSCT was high (62%). The prognosis of AMKL is determined by adverse genetic risk factors and therapy resistance. So far allo-HSCT is the only potentially curative treatment option in this dismal AML subgroup.

Molecular characteristics and zoonotic potential of enteric protists in domestic dogs and cats in Egypt.

Domestic dogs and cats can be a source of human infection by a wide diversity of zoonotic pathogens including parasites. Genotyping and subtyping tools are useful in assessing the true public health relevance of canine and feline infections by these pathogens. This study investigated the occurrence, genetic diversity, and zoonotic potential of common diarrhea-causing enteric protist parasites in household dogs and cats in Egypt, a country where this information is particularly scarce. In this prospective, cross-sectional study a total of 352 individual fecal samples were collected from dogs (n = 218) and cats (n = 134) in three Egyptian governorates (Dakahlia, Gharbeya, and Giza) during July-December 2021. Detection and identification of Cryptosporidium spp., Giardia duodenalis, Enterocytozoon bieneusi, and Blastocystis sp. were carried out by PCR and Sanger sequencing. Basic epidemiological variables (geographical origin, sex, age, and breed) were examined for association with occurrence of infection by enteric protists. The overall prevalence rates of Cryptosporidium spp. and G. duodenalis were 1.8% (95% CI: 0.5-4.6) and 38.5% (95% CI: 32.0-45.3), respectively, in dogs, and 6.0% (95% CI: 2.6-11.4) and 32.1% (95% CI: 24.3-40.7), respectively, in cats. All canine and feline fecal samples analyzed tested negative for E. bieneusi and Blastocystis sp. Dogs from Giza governorate and cats from Dakahlia governorate were at higher risk of infection by Cryptosporidium spp. (p = 0.0006) and G. duodenalis (p = 0.00001), respectively. Sequence analyses identified host-adapted Cryptosporidium canis (n = 4, one of them belonging to novel subtype XXe2) and G. duodenalis assemblages C (n = 1) and D (n = 3) in dogs. In cats the zoonotic C. parvum (n = 5) was more prevalent than host-adapted C. felis (n = 1). Household dogs had a limited (but not negligible) role as source of human giardiasis and cryptosporidiosis, but the unexpected high frequency of zoonotic C. parvum in domestic cats might be a public health concern. This is the first molecular-based description of Cryptosporidium spp. infections in cats in the African continent to date. Molecular epidemiological data provided here can assist health authorities and policy makers in designing and implementing effective campaigns to minimize the transmission of enteric protists in Egypt.

Allopolyploidy: An Underestimated Driver in Juniperus Evolution.

Allopolyploidy is considered as a principal driver that shaped angiosperms' evolution in terms of diversification and speciation. Despite the unexpected high frequency of polyploidy that was recently discovered in the coniferous genus Juniperus, little is known about the origin of these polyploid taxa. Here, we conducted the first study devoted to deciphering the origin of the only hexaploid taxon in Juniperus along with four of its closely related tetraploid taxa using AFLP markers with four primers combinations. Phylogenetic analysis revealed that the 10 studied species belong to 2 major clusters. J. foetidissima appeared to be more related to J. thurifera, J. sabina, and J. chinensis. The Bayesian clustering analysis showing a slight variation in genetic admixture between the studied populations of J. foetidissima, suggesting an allopolyploid origin of this species involving J. thurifera and J. sabina lineages, although a purely autopolyploidy origin of both J. thurifera and J. foetidissima cannot be ruled out. The admixed genetic pattern revealed for J. seravschanica showed that the tetraploid cytotypes of this species originated from allopolyploidy, whereas no clear evidence of hybridization in the origin of the tetraploid J. thurifera and J. chinensis was detected. This study provides first insights into the polyploidy origin of the Sabina section and highlights the potential implication of allopolyploidy in the evolution of the genus Juniperus. Further analyses are needed for a more in-depth understanding of the evolutionary scenarios that produced the observed genetic patterns.

Is There a Semi‐Molten Layer at the Base of the Lunar Mantle?

AbstractParameterised by the Love number k2 and the tidal quality factor Q, and inferred from lunar laser ranging (LLR), tidal dissipation in the Moon follows an unexpected frequency dependence often interpreted as evidence for a highly dissipative, melt‐bearing layer encompassing the core‐mantle boundary. Within this, more or less standard interpretation, the basal layer's viscosity is required to be of order 1015–1016 Pa s and its outer radius is predicted to extend to the zone of deep moonquakes. While the reconciliation of those predictions with the mechanical properties of rocks might be challenging, alternative lunar interior models without the basal layer are said to be unable to fit the frequency dependence of tidal Q. The purpose of our paper is to illustrate under what conditions the frequency‐dependence of lunar tidal Q can be interpreted without the need for deep‐seated partial melt. Devising a simplified lunar model, in which the mantle is described by the Sundberg‐Cooper rheology, we predict the relaxation strength and characteristic timescale of elastically accommodated grain boundary sliding in the mantle that would give rise to the desired frequency dependence. Along with developing this alternative model, we test the traditional model with a basal partial melt; and we show that the two models cannot be distinguished from each other by the available selenodetic measurements. Additional insight into the nature of lunar tidal dissipation can be gained either by measurements of higher‐degree Love numbers and quality factors or by farside lunar seismology.

Refugees versus ‘refugees’: the role of Islamophobia in Swedish alternative media’s reporting on Ukrainian asylum seekers

This paper analyses how immigrants are understood by Swedish alternative media and the role that Islamophobia plays, if at all, in these representations. What is remarkable is that although all articles were sampled explicitly to discuss Ukraine, the analysis showed that Muslim immigrants figured with unexpected frequency throughout. The value of these two immigrant groups were antagonistically contrasted through arguments of alleged differences in culture and geographical origin, perceived legitimacy as asylum seekers, and in terms gratitude and supposed level of threat to Swedish society. With this, the unity that is formed around Islamophobia trumps any nationalist views of the Swedish nation state as particularly superior or white and the social and economic consequences which are usually believed to be at risk due to immigration. By extension, the war in Ukraine is articulated as a matter of whiteness and works to exploit war for strengthening the transnational far right.

Investigation on a coaxial HPM generator with low magnetic field

A Ku band coaxial high power microwave generator with a low magnetic field was investigated without mode competition in this paper. Mode competition is avoided physically by adopting a two-cavity extractor instead of a three-cavity extractor to eliminate the resonance between the modulation slow wave structure and the extractor slow wave structure at any unexpected frequency except the working frequency. Moreover, the efficiency of the device increases due to the separation process of energy extraction and electron collection by adopting the inner conductor collecting structure. Particle simulation results showed that when the magnetic flux density is 0.6 T, the diode voltage is 560 kV, and the diode current is 8 kA, we get 1.55 GW microwaves with an efficiency of 35% and a frequency of 14.7 GHz. Experimental investigation is carried out based on the physical design and simulation results. 1.0 GW microwaves are obtained, and the frequency is 14.8 GHz in the experiment when the magnetic flux density is 0.64 T, the diode voltage is 590 kV, and the diode current is 8.5 kA. No obvious hint of electron emission or bombardment is observed at the surface of the generator after 10 thousand pulses, and the advantage of this kind of generator working in a low magnetic field is proved.

Passivity-Based Stability Analysis and Generic Controller Design for Grid-Forming Inverter

With the remarkable growth of renewable energy resources, the grid-forming (GFM) inverter with the function of grid voltage/frequency support attracts much attention. Due to the inverter-grid interaction, the stability of the GFM inverter is a critical issue. The passivity-based analysis approach, which was widely applied to the conventional grid-following inverter, has been proved to be promising. Yet, its application to the GFM inverter is still insufficient. To this end, this paper conducts a comprehensive passivity-based analysis for the GFM inverter with single-loop voltage control. It finds that the two indices of the passivity-based stability criterion, i.e., the individual stability and the output impedance passivity, bring identical constraints on the voltage controller, and the passivity cannot be ensured with typical voltage controllers. To shrink the unexpected non-passive frequency ranges, a generic grid-current feedforward scheme is explored, and the proper feedforward functions compatible with different voltage controllers are derived. With the proposed scheme, the passivity can be guaranteed up to the Nyquist frequency. Finally, experimental results from a 6-kVA prototype are provided to verify the theoretical analysis.

Unexpected frequency of the pathogenic AR CAG repeat expansion in the general population.

CAG repeat expansions in exon 1 of the AR gene on the X chromosome cause spinal and bulbar muscular atrophy, a male-specific progressive neuromuscular disorder associated with a variety of extra-neurological symptoms. The disease has a reported male prevalence of approximately 1:30 000 or less, but the AR repeat expansion frequency is unknown. We established a pipeline, which combines the use of the ExpansionHunter tool and visual validation, to detect AR CAG expansion on whole-genome sequencing data, benchmarked it to fragment PCR sizing, and applied it to 74 277 unrelated individuals from four large cohorts. Our pipeline showed sensitivity of 100% [95% confidence interval (CI) 90.8-100%], specificity of 99% (95% CI 94.2-99.7%), and a positive predictive value of 97.4% (95% CI 84.4-99.6%). We found the mutation frequency to be 1:3182 (95% CI 1:2309-1:4386, n = 117 734) X chromosomes-10 times more frequent than the reported disease prevalence. Modelling using the novel mutation frequency led to estimate disease prevalence of 1:6887 males, more than four times more frequent than the reported disease prevalence. This discrepancy is possibly due to underdiagnosis of this neuromuscular condition, reduced penetrance, and/or pleomorphic clinical manifestations.

Unexpected high frequency of anti-thyroid peroxidase (anti-TPO) antibodies in Golestan province, Iran.

Anti-TPO antibodies are one of the characteristic factors in autoimmune thyroiditis (AIT). Previous studies reported a high prevalence of anti-TPO antibodies (Abs) in Iran. We have therefore assessed the prevalence of anti-TPO Abs in Gorgan, Iran. This cross-sectional study, conducted from 2015 to 2018 in Gorgan city, Northeast of Iran. The Participants included women with Poly cystic ovary syndrome (PCOs), celiac patients, men with hepatitis C infection, and age and sex-matched controls. ELISA method was used for the analysis of laboratory tests. The number of enrolled subjects in PCOs, celiac disease, and Hepatitis C infection groups were 76, 67, and 60, respectively. Anti-TPO Abs positivity was significantly higher in patients with PCOS than in the control group (18.4% vs. 0.00%; p = 0.000). There were no significant differences in the frequency of anti-TPO Abs positive cases between CD patients and the controls (26.9% vs. 21.1% p =0.413). The incidence of anti-TPO Abs positivity was significantly higher in the control group (10% vs. 25%; P = 0.031). Very high level of anti-TPO Abs was observed in both patients and healthy population in Golestan province. Considering this rate and its association with autoimmune disorders, it is suggested to prioritize screening programs for related disease in this area.

Unexpected phase-locked Brillouin Kerr Frequency comb in fiber Fabry Perot resonators

We report the observation of a stable and broadband optical frequency comb in a high-Q fiber Fabry Perot resonator. We evidence it arises from an unexpected mode-locking phenomena.