Introduction: Neonatal Intestinal obstruction is commonest surgical cause in emergency, in which rapid intervention is required to prevent undesirable side effect of obstruction that includes death. This study provides spectrum of intestinal obstruction in neonates and sex prediction. Material and Method: This is prospective study done in neonates with feature of intestinal obstruction attending emergency and outdoor of Pediatrics Surgery, Pediatrics Medicine and General Surgery of U.P.U.M.S , Saifai, Etawah during period of January 2016–December 2016. Result: During the study period of one year total 44 patents admitted to our side with features of neonatal obstruction in which male female ratio was 3.8:1. Out of these 44 neonates Anorectal malformation was found in 61.36% neonates, Hirschsprung’s Disease in 9.09%, Duodenal obstruction in 6.81%, Hernia of umbilical cord in 4.54%, Atresia in 4.54, Congenital peritoneal band in 2.27%, Meconium obstruction in 2.27%, Malrotation in 2.27%, Meckel’s band obstruction in 2.27%, Ileal knotting in 2.27%, Idiopathic hypertrophic pyloric stenosis (IHPS) in 2.27%. Conclusion: Early identification and surgical management in neonatal intestinal obstruction decrease morbidity and motilities in neonates. Reason of male sex predominance is not known, it may be due to less attention towards female child by social custom in our society.