The fatigue life of surface layer (FSL) is innovatively characterized to accurately capture the effect of surface integrity on the fatigue behavior of the components. The sensitivity of FSL of 300 M ultrahigh strength steel to the surface integrity indexes induced by representative manufacturing processes was analyzed by analytical modeling and fatigue tests from the perspective of engineering fracture mechanics. The ultrasonic surface rolling process (USRP) was introduced to optimize the fatigue limit of 300 M steel, and the improvement in fatigue life was experimentally quantified. Besides, the fracture analysis was conducted to provide the reason why USRP can enhance the fatigue behavior of 300 M steel. The results show that FSL occupies a majority with percentage of over 95 % in the entire fatigue life and a strong relation with the machined surface defects, especially the surface machining marks. The excellent surface finishing effect and high compressive residual stresses induced by engineered USRP could transfer the crack source from surface machining marks into subsurface inclusions, which greatly prolongs the fatigue crack initiation life and thereby the entire fatigue life. To be specific, the fatigue life was elevated by more than 40 times and the fatigue strength was increased by 34.7 % after USRP for the tested 300 M steel.