Ultrasonic-assisted Solid-phase Extraction Research Articles

Ultrasonic-assisted solid-phase extraction of sotalol in human urine samples using molecularly imprinted nanoparticles: Experimental design and adsorption study

ABSTRACTUltrasonic-assisted solid-phase extraction via molecularly imprinted nanoparticles followed by HPLC-UV was used for trace determination of sotalol. Under optimized conditions, linear range, limit of detection, and limit of quantification of the proposed method were found to be 20–2000, 7.8, and 24 ng/mL, respectively. The adsorption capacity of sotalol for molecularly imprinted nanoparticles and non-imprinted nanoparticles was determined from the derived Langmuir isotherm and was found to be 31.55 and 15.22 mg/g, respectively. The proposed method was used for the separation of trace amounts of sotalol in human urine samples with acceptable high recoveries more than 94.40% and the inter-day precision with the coefficient of variation lower than 5%.

Synthesis and application of molecularly imprinted nanoparticles combined ultrasonic assisted for highly selective solid phase extraction trace amount of celecoxib from human plasma samples using design expert (DXB) software

In this work molecular imprinted nanoparticles (MINPs) was synthesized and applied for ultrasonic assisted solid phase extraction of celecoxib (CEL) from human plasma sample following its combination by HPLC–UV. The MINPs were prepared in a non-covalent approach using methacrylic acid as monomer, CEL as template, ethylene glycol dimethacrylate as cross-linker, and 2,2-azobisisobutyronitrile (AIBN) as the initiator of polymerization. pH, volume of rinsing and eluent solvent and amount of sorbent influence on response were investigated using factorial experimental design, while optimum point was achieved and set as 250mg sorbent, pH 7.0, 1.5mL washing solvent and 2mL eluent by analysis of results according to design expert (DX) software. At above specified conditions, CEL in human plasma with complicated matrices with acceptable high recoveries (96%) and RSD% lower than 10% was quantified and estimated.The proposed MISPE-HPLC–UV method has linear responses among peak area and concentrations of CEL in the range of 0.2–2000μgL−1, with regression coefficient of 0.98. The limit of detection (LOD) and quantification (LOQ) based on three and ten times of the noise of HPLC peaks correspond to blank solution were 0.08 and 0.18μgL−1, respectively.