Ultra-low Field Research Articles

Field cycling imaging to characterise breast cancer at low and ultra-low magnetic fields below 0.2 T

BackgroundThis prospective feasibility study explores Field-Cycling Imaging (FCI), a new MRI technology that measures the longitudinal relaxation time across a range of low magnetic field strengths, providing additional information about the molecular properties of tissues. This study aims to assess the performance of FCI and investigate new quantitative biomarkers at low fields within the context of breast cancer.MethodsWe conducted a study involving 9 people living with breast cancer (10 tumours in total, mean age, 54 ± 10 years). FCI images were obtained at four magnetic field strengths (2.3 mT to 200 mT). FCI images were processed to generate T1 maps and 1/T1 dispersion profiles from regions of tumour, normal adipose tissue, and glandular tissue. The dispersion profiles were subsequently fitted using a power law model. Statistical analysis focused on comparing potential FCI biomarkers using a Mann-Whitney U or Wilcoxon signed rank test.ResultsWe show that low magnetic fields clearly differentiate tumours from adipose and glandular tissues without contrast agents, particularly at 22 mT (1/T1, median [IQR]: 6.8 [3.9–7.8] s−1 vs 9.1 [8.9–10.2] s−1 vs 8.1 [6.2–9.2] s−1, P < 0.01), where the tumour-to-background contrast ratio was highest (62%). Additionally, 1/T1 dispersion indicated a potential to discriminate invasive from non-invasive cancers (median [IQR]: 0.05 [0.03–0.09] vs 0.19 [0.09–0.26], P = 0.038).ConclusionsTo the best of our knowledge, we described the first application of in vivo FCI in breast cancer, demonstrating relevant biomarkers that could complement diagnosis of current imaging modalities, non-invasively and without contrast agents.

The application of PVC hollow fiber ultrafiltration membrane in ultra-low permeability oilfield

Abstract Oil containing wastewater produced from ultra-low permeability oil fields generally needs to be treated to meet the 5-1-1 standard before it can be reinjected. In order to meet the standards for treating oily wastewater in ultra-low permeability oil fields, PVC hollow fiber ultrafiltration membranes and pre-treatment processes for removing oil, suspended solids, sulfides, etc. before the membrane were selected. The application shows that the aeration air flotation flow sand filtration PVC hollow fiber ultrafiltration membrane process can treat oily wastewater, and the treated water can reach the “5-1-1” standard. In the pre-treatment process of PVC hollow fiber ultrafiltration membrane, the silicone rubber membrane aeration device has good oxidation effect, high efficiency in micro nano air flotation for oil removal and suspended solids, and easy management of sand flow filters. This process has economic feasibility and provides technical support for the treatment of oily wastewater in ultra-low permeability oil fields, with great promotional value.

Experimental Study on the Treatment of Produced Water from Carbon Dioxide Drive by Aeration Method

Abstract To further explore the effective development of produced water treatment technologies suitable for low to ultra-low permeability oil fields, and to clarify the water quality characteristics of produced water from CO2 flooding. A study was conducted on the basic water quality characteristics of produced water from low to ultra-low permeability oil fields. Indoor CO2 gas removal experiments were conducted using the aeration method under 40°C atmospheric pressure conditions. The research results indicate that the CO2 content in the extracted water is one of the important influencing factors for the changes in characteristic parameters such as water pH, mineralization, and corrosion rate; Under the condition of 40°C atmospheric pressure test, the aeration method has a significant treatment effect on the produced water from CO2 flooding, which can effectively remove 70% of the CO2 in the water, thereby improving the pH of the water to 8, and effectively inhibiting the corrosion rate of the produced water below 0.076mm/a.

High sensitivity and detectivity of anomalous Hall sensor based on coupled magnetic bilayers

Detection of ultralow magnetic field requires a magnetic sensor with high sensitivity and a low noise level. In this work, we used the Co20Fe60B20/Ti/Co20Fe60B20 magnetically coupled multilayer as the core structure of an anomalous Hall sensor. We adjusted the thickness of the Ti interlayer to modify its perpendicular magnetic anisotropy and interlayer magnetic coupling, thereby improving the sensitivity of the anomalous Hall sensor. Through the investigation of magnetic field response and noise properties of devices with different Ti thicknesses, the highest sensitivity of 34 803 Ω/T and the best magnetic field detectivity of 4.6 nT/Hz at 1 Hz were achieved with a Ti thickness of 2.0 nm at room temperature. This anomalous Hall sensor has both ultrahigh sensitivity and magnetic field detectivity, making it a good candidate for applications in detecting weak magnetic fields.

Enhancing organ and vascular contrast in preclinical ultra-low field MRI using superparamagnetic iron oxide nanoparticles

Superparamagnetic iron oxide nanoparticles (SPIONs) are characterized by their exceptional susceptibility and relaxivity at ultra-low field (ULF) regimes, make them a promising contrast agent (CA) for ULF MRI. Despite their distinct advantages, the translation of these properties into clinically valuable image contrast in ULF MRI remains underexplored. In this study, we investigate the use of SPIONs to generate in vivo MRI contrast at 6.5 mT within the organs and vascular system of rodents. This investigation includes comprehensive SPION characterization and phantom imaging experiments to validate the utility of SPIONs to produce positive image contrast and to facilitate phase-sensitive imaging at ULF. Optimized balanced steady-state free precession (bSSFP) and spoiled gradient echo (SPGR) MRI sequences are used to generate in vivo contrast by leveraging the distinctive properties of SPIONs at ULF. Imaging studies in rodents reveal positive organ contrast attainable in magnitude images, and MRI phase maps can be used to visualize the vascular system. This work demonstrates the effectiveness of SPIONs in enhancing preclinical organ and vascular imaging at ULF; it bridges the gap between the study of the distinctive physical properties of SPIONs and the demonstration of in vivo image contrast.

Achieving a Noise Limit with a Few-layer WSe2 Avalanche Photodetector at Room Temperature.

We engineered a two-dimensional Pt/WSe2/Ni avalanche photodetector (APD) optimized for ultraweak signal detection at room temperature. By fine-tuning the work functions, we achieved an ultralow dark current of 10-14 A under small bias, with a noise equivalent power (NEP) of 8.09 fW/Hz1/2. This performance is driven by effective dark barrier blocking and a record-long electron mean free path (123 nm) in intrinsic WSe2, minimizing dark carrier replenishment and suppressing noise under an ultralow electric field. Our APD exhibits a high gain of 5 × 105 at a modulation frequency of 20 kHz, effectively balancing gain and bandwidth, a common challenge in traditional photovoltaic-based APDs. By addressing the typical challenges of high noise and low gain and minimizing dependence on high electric fields, this work highlights the potential of 2D materials in developing efficient, low-power, and ultrasensitive photodetections.

Ultra-low-field magnetization transfer imaging at 0.055T with low specific absorption rate.

To demonstrate magnetization transfer (MT) effects with low specific absorption rate (SAR) on ultra-low-field (ULF) MRI. MT imaging was implemented by using sinc-modulated RF pulse train (SPT) modules to provide bilateral off-resonance irradiation. They were incorporated into 3D gradient echo (GRE) and fast spin echo (FSE) protocols on a shielding-free 0.055T head scanner. MT effects were first verified using phantoms. Brain MT imaging was conducted in both healthy subjects and patients. MT effects were clearly observed in phantoms using six SPT modules with total flip angle 3600° at central primary saturation bands of approximate offset ±786 Hz, even in the presence of large relative B0 inhomogeneity. For brain, strong MT effects were observed in gray matter, white matter, and muscle in 3D GRE and FSE imaging using six and sixteen SPT modules with total flip angle 3600° and 9600°, respectively. Fat, cerebrospinal fluid, and blood exhibited relatively weak MT effects. MT preparation enhanced tissue contrasts in T2-weighted and FLAIR-like images, and improved brain lesion delineation. The estimated MT SAR was 0.0024 and 0.0008 W/kg for two protocols, respectively, which is far below the US Food and Drug Administration (FDA) limit of 3.0 W/kg. Robust MT effects can be readily obtained at ULF with extremely low SAR, despite poor relative B0 homogeneity in ppm. This unique advantage enables flexible MT pulse design and implementation on low-cost ULF MRI platforms to achieve strong MT effects in brain and beyond, potentially augmenting their clinical utility in the future.

Ultra-low-field magnetic resonance angiography at 0.05 T: A preliminary study.

We aim to explore the feasibility of head and neck time-of-flight (TOF) magnetic resonance angiography (MRA) at ultra-low-field (ULF). TOF MRA was conducted on a highly simplified 0.05 T MRI scanner with no radiofrequency (RF) and magnetic shielding. A flow-compensated three-dimensional (3D)gradient echo (GRE) sequence with a tilt-optimized nonsaturated excitation RF pulse, and a flow-compensated multislice two-dimensional (2D) GRE sequence, were implemented for cerebral artery and vein imaging, respectively. For carotid artery and jugular vein imaging, flow-compensated 2D GRE sequences were utilized with venous and arterial blood presaturation, respectively. MRA was performed on young healthy subjects. Vessel-to-background contrast was experimentally observed with strong blood inflow effect and background tissue suppression. The large primary cerebral arteries and veins, carotid arteries, jugular veins, and artery bifurcations could be identified in both raw GRE images and maximum intensity projections. The primary brain and neck arteries were found to be reproducible among multiple examination sessions. These preliminary experimental results demonstrated the possibility of artery TOF MRA on low-cost 0.05 T scanners for the first time, despite the extremely low MR signal. We expect to improve the quality of ULF TOF MRA in the near future through sequence development and optimization, ongoing advances in ULF hardware and image formation, and the use of vascular T1 contrast agents.

Homogeneous B0 coil design method for open-access ultra-low field magnetic resonance imaging: A simulation study

A multimodal brain function measurement system integrating functional magnetic resonance imaging (fMRI) and magnetoencephalography (MEG) is expected to be a tool that will provide new insights into neuroscience. To integrate fMRI and MEG, an ultra-low-field MRI (ULF-MRI) scanner that can generate a static magnetic field (B0) with an electromagnetic coil and turn off the B0 during MEG measurements is desirable. While electromagnetic B0 coil has the above advantages, it also has a trade-off between size and the broadness of the magnetic field homogeneity. In this study, we proposed a method for designing a B0 multi-stage circular coil arrangement that determines the number of coils required to maximize magnetic field homogeneity and minimize the total wiring length of the coils. The optimized multi-stage coil arrangement had an external shape of 600 mm in diameter and a maximum height of 600 mm, with an aperture of 600 mm in diameter and 300 mm in height. The magnetic field homogeneity was <100 ppm over a 210 mm diameter spherical volume (DSV). Compared to a previous two coil pairs arrangement with the same magnetic field homogeneity, the diameter was 1/1.9 times smaller, indicating that the newly designed B0 coil arrangement realized a smaller size and wider magnetic field homogeneity.

Piezoelectric MEMS resonator with magnetic tip mass for energy harvesting from ultra-low intensity magnetic fields

This article addresses the limitation in the implementation of the Internet of Things (IoT) due to its energy dependence on batteries. The central focus of this work is the development and characterization of a magnetoelectric (ME) energy generator for IoT, which combines a piezoelectric microelectromechanical system (MEMS) with a magnetic mass to interact with ambient magnetic fields (MFs). The device's efficiency is validated through finite element modeling (FEM) simulations and electrical characterization. Its applicability in environments with residual magnetic fields, such as near common household appliances, highlights its viability. Specific results, such as a maximum generated power of 0.55 µW, corresponding to a power density of 9.19 µW/cm³, for a magnetic field strength of 5 µT, emphasize its ability to address energy challenges and promote the sustainable autonomy of IoT.

Spatial evolution of the proton-coupled Mott transition in correlated oxides for neuromorphic computing.

The proton-electron coupling effect induces rich spectrums of electronic states in correlated oxides, opening tempting opportunities for exploring novel devices with multifunctions. Here, via modest Pt-aided hydrogen spillover at room temperature, amounts of protons are introduced into SmNiO3-based devices. In situ structural characterizations together with first-principles calculation reveal that the local Mott transition is reversibly driven by migration and redistribution of the predoped protons. The accompanying giant resistance change results in excellent memristive behaviors under ultralow electric fields. Hierarchical tree-like memory states, an instinct displayed in bio-synapses, are further realized in the devices by spatially varying the proton concentration with electric pulses, showing great promise in artificial neural networks for solving intricate problems. Our research demonstrates the direct and effective control of proton evolution using extremely low electric field, offering an alternative pathway for modifying the functionalities of correlated oxides and constructing low-power consumption intelligent devices and neural network circuits.

Zero to ultralow magnetic field NMR of [1−13C]pyruvate and [2−13C]pyruvate enabled by SQUID sensors and hyperpolarization

Accurately characterizing magnetic resonance of molecules at zero to ultralow magnetic field (nTs-µTs) is challenging, due to vanishingly small sensitivity, which depends on the thermal equilibrium polarization of the nuclear spins and instrumentation. We overcome the former limitation with the parahydrogen-based hyperpolarization method SABRE-SHEATH (signal amplification by reversible exchange in shield enables alignment transfer to heteronuclei). This method allows for the continuous transfer of spin order from parahydrogen to a substrate via chemical exchange, reaching polarization levels of some percent (level equivalent to C13 polarization at 20 kT). We address the latter with our application of a superconducting quantum interference device (SQUID)-based detector setup that allows for broadband detection (dc-MHz) with exquisite sensitivity over its entire range. Here, we present the results of our comprehensive characterization of [1−13C]pyruvate and [2−13C]pyruvate, hyperpolarized via SABRE-SHEATH, from zero field to 100 µT. To this end, we show low-noise, high-resolution spectra for both molecules, detecting how the NMR spectrum changes from the -coupling dominated zero-field spectrum to the strongly coupled spectrum, and then finally to the conventional high-field, otherwise known Zeeman-dominated spectrum. We also analytically derive the evolution of product operators in arbitrary magnetic fields, which aid in the understanding of the differences between spin evolution and spin-coupling regimes. We predict and confirm that the absence of spin precession at zero field can result in observable oscillation of magnetization along one axis with a frequency of the -coupling constant, no observable spin evolution, or observing spin evolution that corresponds to “forbidden” transitions at high field. The zero-field spectra with their near-dc signals reveal different relaxation rates for the different spin states of hyperpolarized C13 pyruvates, demonstrating the utility of SQUID detectors and hyperpolarization for the characterization of magnetic resonance phenomena. Published by the American Physical Society 2024

Super resolution using sparse sampling at portable ultra-low field MR

Ultra-low field (ULF) magnetic resonance imaging (MRI) holds the potential to make MRI more accessible, given its cost-effectiveness, reduced power requirements, and portability. However, signal-to-noise ratio (SNR) drops with field strength, necessitating imaging with lower resolution and longer scan times. This study introduces a novel Fourier-based Super Resolution (FouSR) approach, designed to enhance the resolution of ULF MRI images with minimal increase in total scan time. FouSR combines spatial frequencies from two orthogonal ULF images of anisotropic resolution to create an isotropic T2-weighted fluid-attenuated inversion recovery (FLAIR) image. We hypothesized that FouSR could effectively recover information from under-sampled slice directions, thereby improving the delineation of multiple sclerosis (MS) lesions and other significant anatomical features. Importantly, the FouSR algorithm can be implemented on the scanner with changes to the k-space trajectory. Paired ULF (Hyperfine SWOOP, 0.064 tesla) and high field (Siemens, Skyra, 3 Tesla) FLAIR scans were collected on the same day from a phantom and a cohort of 10 participants with MS or suspected MS (6 female; mean ± SD age: 44.1 ± 4.1). ULF scans were acquired along both coronal and axial planes, featuring an in-plane resolution of 1.7 mm × 1.7 mm with a slice thickness of 5 mm. FouSR was evaluated against registered ULF coronal and axial scans, their average (ULF average) and a gold standard SR (ANTs SR). FouSR exhibited higher SNR (47.96 ± 12.6) compared to ULF coronal (36.7 ± 12.2) and higher lesion conspicuity (0.12 ± 0.06) compared to ULF axial (0.13 ± 0.07) but did not exhibit any significant differences contrast-to-noise-ratio (CNR) compared to other methods in patient scans. However, FouSR demonstrated superior image sharpness (0.025 ± 0.0040) compared to all other techniques (ULF coronal 0.021 ± 0.0037, q = 5.9, p-adj. = 0.011; ULF axial 0.018 ± 0.0026, q = 11.1, p-adj. = 0.0001; ULF average 0.019 ± 0.0034, q = 24.2, p-adj. &amp;lt; 0.0001) and higher lesion sharpness (−0.97 ± 0.31) when compared to the ULF average (−1.02 ± 0.37, t(543) = −10.174, p = &amp;lt;0.0001). Average blinded qualitative assessment by three experienced MS neurologists showed no significant difference in WML and sulci or gyri visualization between FouSR and other methods. FouSR can, in principle, be implemented on the scanner to produce clinically useful FLAIR images at higher resolution on the fly, providing a valuable tool for visualizing lesions and other anatomical structures in MS.

Giant magneto-photoluminescence at ultralow field in organic microcrystal arrays for on-chip optical magnetometer

Optical detection of magnetic field is appealing for integrated photonics; however, the light-matter interaction is usually weak at low field. Here we observe that the photoluminescence (PL) decreases by > 40% at 10 mT in rubrene microcrystals (RMCs) prepared by a capillary-bridge assembly method. The giant magneto-PL (MPL) relies on the singlet-triplet conversion involving triplet-triplet pairs, through the processes of singlet fission (SF) and triplet fusion (TF) during radiative decay. Importantly, the size of RMCs is critical for maximizing MPL as it influences on the photophysical processes of spin state conversion. The SF/TF process is quantified by measuring the prompt/delayed PL with time-resolved spectroscopies, which shows that the geminate SF/TF associated with triplet-triplet pairs are responsible for the giant MPL. Furthermore, the RMC-based magnetometer is constructed on an optical chip, which takes advantages of remarkable low-field sensitivity over a broad range of frequencies, representing a prototype of emerging opto-spintronic molecular devices.

Near-collapse of the geomagnetic field may have contributed to atmospheric oxygenation and animal radiation in the Ediacaran Period

Earth’s magnetic field was in a highly unusual state when macroscopic animals of the Ediacara Fauna diversified and thrived. Any connection between these events is tantalizing but unclear. Here, we present single crystal paleointensity data from 2054 and 591 Ma pyroxenites and gabbros that define a dramatic intensity decline, from a strong Proterozoic field like that of today, to an Ediacaran value 30 times weaker. The latter is the weakest time-averaged value known to date and together with other robust paleointensity estimates indicate that Ediacaran ultra-low field strengths lasted for at least 26 million years. This interval of ultra-weak magnetic fields overlaps temporally with atmospheric and oceanic oxygenation inferred from numerous geochemical proxies. This concurrence raises the question of whether enhanced H ion loss in a reduced magnetic field contributed to the oxygenation, ultimately allowing diversification of macroscopic and mobile animals of the Ediacara Fauna.

552: First-in-human breast imaging study with ultra-low field MRI for compact proton therapy systems

552: First-in-human breast imaging study with ultra-low field MRI for compact proton therapy systems

Phase-field simulation and machine learning of low-field magneto-elastocaloric effect in a multiferroic composite

Achieving appreciable elastocaloric effect under low external field is critical for solid-state cooling technology. Here, a non-isothermal Phase-Field Model (PFM) coupling martensitic transformation with mechanics, heat transfer and magnetostrictive behavior is proposed to simulate Magneto-elastoCaloric Effect (M-eCE) that is induced by magnetic field in a multiferroic composite (e.g., Magnetostrictive-Shape Memory Alloys (MEA-SMA) composite). In the PFM, a nonlinear constitutive hyperbolic tangent model is utilized to model the macroscopic magnetostrictive behavior of MEA, and the heat transfer coupled with phase transformation is employed to calculate the adiabatic temperature change (ΔTad) during M-eC cooling cycles. The influences of magnetic field, geometrical dimension, and ambient temperature on ΔTad are comprehensively investigated. Machine Learning (ML) is further conducted on the database from PFM simulations to accelerate the prediction and design of MEA-SMA composite with an improved ΔTad. It is found that a large ΔTad of 10–14 K and a wide working temperature window of 30 K can be achieved under ultra-low magnetic field of 0.15–0.38 T by optimizing the composite’s geometrical dimension. The present work combining PFM and ML for evaluating M-eCE provides a theoretical framework for the optimization of M-eC cooling devices, and is also potentially extended to other multicaloric effects (e.g., electro-elastocaloric effect).

Two fields are better than one – A multifunctional (semi)automated setup for quantitative measurements of parahydrogen-induced signal enhancement at low and high fields

The rapid advancement of parahydrogen-induced hyperpolarization (PHIP) and its diverse array of applications highlights the critical need for enhanced signals in both 1H NMR and heteronuclear NMR spectroscopy. Simultaneously, there is an increasing interest in utilizing benchtop NMR analysis across various laboratory settings. However, due to their lower magnetic fields, benchtop NMR spectrometers inherently produce weaker signal intensities. Here, PHIP is a well-established solution to this challenge. Consequently, we are expanding our cost-effective PHIP setup from a high-field NMR spectrometer (11.7 T) to include an additional benchtop NMR spectrometer (1.4 T), thereby enabling concurrent execution of PHIP experiments and measurements. Through the implementation of automated experimental protocols, we aim to minimize experiment time while increasing reproducibility. In this work, a non-isotope labelled propargyl alcohol sample is used at low concentrations to demonstrate our setup’s capabilities. It could be shown that single-scan PASADENA experiments can be run with comparable signal enhancements at the benchtop as well as the high-field spectrometer. At 1.4 T, fully automated PHIP pseudo-2D measurements will also be demonstrated. Additionally, two different field profiles for the spin-order transfer of p-H2 to 13C at zero- to ultralow fields are elaborated upon. The setup facilitates the measurement of carbon signal enhancement of more than 2000 on the benchtop NMR spectrometer, employing a straightforward one-pulse, one-scan experiment.

Contrast Agents Based on Human Serum Albumin and Nitroxides for 1H-MRI and Overhauser-Enhanced MRI.

In cancer diagnostics, magnetic resonance imaging (MRI) uses contrast agents to enhance the distinction between the target tissue and background. Several promising approaches have been developed to increase MRI sensitivity, one of which is Overhauser dynamic nuclear polarization (ODNP)-enhanced MRI (OMRI). In this study, a macromolecular construct based on human serum albumin and nitroxyl radicals (HSA-NIT) was developed using a new synthesis method that significantly increased the modification to 21 nitroxide residues per protein. This was confirmed by electron paramagnetic resonance (EPR) spectroscopy and matrix-assisted laser desorption/ionization time-of-flight (MALDI ToF) mass spectrometry. Gel electrophoresis and circular dichroism showed no significant changes in the structure of HSA-NITs, and no oligomers were formed during modification. The cytotoxicity of HSA-NITs was comparable to that of native albumin. HSA-NITs were evaluated as potential "metal-free" organic radical relaxation-based contrast agents for 1H-MRI and as hyperpolarizing contrast agents for OMRI. Relaxivities (longitudinal and transversal relaxation rates r1 and r2) for HSA-NITs were measured at different magnetic field strengths (1.88, 3, 7, and 14 T). Phantoms were used to demonstrate the potential use of HSA-NIT as a T1- and T2-weighted relaxation-based contrast agent at 3 T and 14 T. The efficacy of 1H Overhauser dynamic nuclear polarization (ODNP) in liquids at an ultralow magnetic field (ULF, B0 = 92 ± 0.8 μT) was investigated for HSA-NIT conjugates. The HSA-NITs themselves did not show ODNP enhancement; however, under the proteolysis conditions simulating cancer tissue, HSA-NIT conjugates were cleaved into lower-molecular-weight (MW) protein fragments that activate ODNP capabilities, resulting in a maximum achievable enhancement |Emax| of 40-50 and a radiofrequency power required to achieve half of Emax, P1/2, of 21-27 W. The HSA-NIT with a higher degree of modification released increased the number of spin probes upon biodegradation, which significantly enhanced the Overhauser effect. Thus, HSA-NITs may represent a new class of MRI relaxation-based contrast agents as well as novel cleavable conjugates for use as hyperpolarizing contrast agents (HCAs) in OMRI.

SABRE-hyperpolarization dynamics of [1-13C]pyruvate monitored by in situ zero- to ultra-low field NMR

Hyperpolarized [1–13C]pyruvate is the leading metabolite used in the emerging field of hyperpolarization-enhanced MRI. Signal amplification by reversible exchange (SABRE) is a straight forward hyperpolarization method that has recently been shown to hyperpolarize [1–13C]pyruvate at low (microtesla and below) magnetic fields. Here, we show that commercial optical magnetometers with Rb-vapor media can be used to readily monitor the build-up and decay of the hyperpolarized MR signal. In addition, we measure ZULF-NMR spectra in various conditions, ranging from a J-coupling-dominated regime transitioning into a Zeeman-dominated regime when going from a sub-nT field to a µT field. The experimentally acquired spectra are matched well by numerical simulations.