Ulleung Island Research Articles

The complete chloroplast genome sequence of Phedimus Kamtschaticus (Crassulaceae) in Korea

The complete chloroplast genome sequence of Phedimus kamtschaticus, which commonly occurs in northeastern Asia was determined. The genome size was 151,652 bp, composed of one pair of inverted repeats (IRs) of 25,977 bp, which were separated by one large single-copy (LSC; 83,010 bp) and one small single-copy (SSC; 16,688 bp) region. The chloroplast genome contained 132 genes, including 88 protein-coding genes, 36 tRNA genes, and 8 rRNA genes. The overall GC content was 37.8%. Phylogenetic analysis of the complete chloroplast genome suggested that P. kamtschaticus was most closely related to Ulleung Island insular endemic P. takesimensis.

Propagation and restoration of destroyed original habitat of Adenophora erecta S. Lee, Joongku Lee et S. Kim, an endemic species of Ulleung island in Korea

Propagation and restoration of destroyed original habitat of Adenophora erecta S. Lee, Joongku Lee et S. Kim, an endemic species of Ulleung island in Korea

A New Species and Introgression in Eastern Asian Hemlocks (Pinaceae: Tsuga)

Species delimitation in Pinaceae is often challenged by limited morphological differentiation and introgression. In Tsuga (hemlocks), species delimitation has been most challenging among northeastern Asian taxa, where the species are weakly marked morphologically and range in number from three to five in previous studies. Two low-copy nuclear four-coumarase-ligase (4CL) genes andmorphology strongly support a clade of the Japanese endemic T. diversifolia and T. sieboldii from Japan and Ulleung island (Ulleungdo) in Korea. This clade is here referred to as the oceanic hemlocks. 4CL strongly supports a sister-group relationship of the widespread northeastern Asian T. chinensis and eastern North American T. caroliniana. In contrast, chloroplast genomes, which are markedly reduced in Tsuga and relatives, strongly support Japanese T. sieboldii as sister to T. chinensis and moderately support T. caroliniana as sister to a clade of T. diversifolia and hemlocks fromUlleungdo. These divergent topologies suggest chloroplast capture of T. chinensis by Japanese T. sieboldii. Ulleungdo hemlocks are distinct from other northeastern Asian species in leaf and cone morphology and phenologically in common-garden observations. We therefore describe these hemlocks as a new species, T. ulleungensis.

Ten New Recorded Species of Macrofungi on Ulleung Island, Korea

Ulleung Island is a biodiversity hotspot in South Korea. During a survey of indigenous fungal species from Ulleung Island conducted from 2015 to 2016, we discovered 10 unrecorded macrofungi in Korea. These macrofungi were identified to the species level using morphological features and phylogenetic analysis based on the internal transcribed spacer region: Deconica phyllogena, Mycena zephirus, Phaeomarasmius proximans, Phlebia radiata, Pluteus semibulbosus, Postia alni, Resinicium pinicola, Scytinostroma portentosum, Tricholomopsis flammula, and Tyromyces kmetii. We also provide detailed morphological descriptions for these 10 species.

Chromosomal characterization based on repetitive DNA distribution in a tetraploid cytotype of Chrysanthemum zawadskii

The aim of this study was to gain insight into the basic genetics of Chrysanthemum zawadskii Herbich ssp. lucidum (NAK.) Y. Lee, a Chrysanthemum subspecies from Ulleung Island, South Korea, by investigating its karyotypic and cytogenetic characteristics, and to contribute to the karyosystematic study of the genus Chrysanthemum (tribe: Anthemideae). Fluorescence in situ hybridization (FISH) was used to characterize the ploidy level, chromosome constitution, and distribution of 5S and 18S ribosomal DNA (rDNA) and Arabidopsis-type telomere repeats (TTTAGGG)n in C. zawadskii ssp. lucidum chromosomes. The somatic metaphase chromosome number of C. zawadskii ssp. lucidum was tetraploid (2n = 4x = 36). Signals from four 5S rDNA loci were detected in the middle of the long arms of some chromosomes, whereas signals from eight 18S rDNA loci were located at the termini of the short arms. The two rDNA markers localized to different chromosomes with no co-localization. The Arabidopsis-type telomeric repeats, however, co-localized with 18S rDNA sites at the ends of both chromosomal arms. This co-localization of telomeric sequences and 18S rDNA has also been observed in other Chrysanthemum species with various levels of ploidy, indicating that it is a general feature of the genus. DAPI (4′,6-diamidino-2-phenylindole) bands incidentally detected after FISH are also illustrated. These preliminary data contribute towards understanding the karyotypic trends and role of polyploidy in the evolution of the genus Chrysanthemum.

Development of nuclear microsatellite markers for the Japanese conifers Tsuga diversifolia and T. sieboldii (Pinaceae)

AbstractNuclear microsatellite markers were developed for the two Tsuga species native to the Japanese Archipelago, Tsuga diversifolia and T. sieboldii, and a population with genetic affinities to T. diversifolia on Ulleung Island, Korea. Tsuga diversifolia and T. sieboldii are widespread dominant trees of temperate and subalpine forests in Japan but to date no genetic markers have been developed for these species. Fifteen polymorphic loci were developed and characterized, of which 14 are reliably amplified in each taxon. Across both species and the Ulleung Island population, the number of alleles per locus ranged from 3 to 26 (average = 13.93) and observed heterozygosity ranged from 0.005 to 0.935 (average = 0.535). In addition, all 15 loci were successfully amplified in a single accession of the Chinese species, T. chinensis. These markers will be useful for investigating the species’ biogeography, range‐wide genetic diversity, conservation genetic issues and potential for hybridisation.

Annual gametogenesis and reproductive effort of the limpet Cellana grata (Gould, 1859) (Gastropoda: Nacellidae) in a rocky intertidal beach at Ulleungdo Island off the east coast of Korea

Widely distributed from the northern coast of Vietnam to the northern Japan, the limpet Cellana grata (Gould, 1859) occurs commonly on the south and east coasts of Korea. Despite their wide distribution range, few studies have investigated the annual gametogenesis and reproductive effort of C. grata. In an attempt to understand the reproductive physiology of the limpet, we investigated the annual gametogenesis and reproductive effort of C. grata from Ulleungdo Island off the east coast of Korea. Histology revealed that the gonial mitosis commenced in January, as the female exhibited small oogonia (10–40 μm) in the follicle. From March to June, the oocyte size increased dramatically, and fully mature eggs (110–170 μm in diameter) appeared in early summer. First spawning males and females were observed in July, as the surface seawater temperature (SST) reached 22.1°C. The spawning male and females could be observed until the end of December. Gonad somatic index (GSI), a ratio of gonad mass to the total tissue weight, of the male ranged from 0.6 (April) to 17.9 (July), while the female GSI varied from 1.0 (February) to 18.3 (July). GSI of male and female declined rapidly from July to August, suggesting that the major purse of the spawning at the study site was between July and August. Our study suggested that the commercial catch of C. grata during July and August must be suspended at Ulleungdo Island, in order to protect the spawning limpets, which enhances C. grata recruitment and the population.

울릉도 곰바위용결응회암 내 흑요암의 산출특징과 성인

이 연구의 목적은 울릉도 남서부 지역에 분포하는 흑요암의 화산학적, 광물학적 특징을 통하여 흑요암의 성인과 산출의미를 고찰하는 데 있다. 흑요암은 곰바위용결응회암 하부에서 국부적으로 발달하며, 다양하고 복잡한 조직과 유동띠가 관찰된다. 흑요암은 알칼리장석 반정과 응회암 및 조면암의 암편과 혼재하여 산출된다. 흑요암은 파동형, 렌즈상의 덩어리로 산출되거나 파쇄된 흑요암 입자들로 인해 얇은 층리로 발달한다. 진주상 균열, 둥근 또는 구형균열과 같은 냉각균열이 특징적으로 발달하는데, 이는 빠르게 냉각하여 규산질이 풍부한 비정질 상태의 흑요암이 형성되었음을 지시한다. 수화작용은 흑요암의 가장자리에서 상대적으로 현저하여 변질띠를 이룬다. 흑요암의 유리질 기질에는 자형의 새니딘과 아노소클레이스 같은 알칼리장석 반정 외에도, 투휘석, 흑운모, 티탄철석, 철산화물 등이 수반된다. 특히 산점상의 미세결정들은 대부분 알칼리장석류와 티탄철석으로 구성된다. EPMA 정량분석 결과, 흑요암의 유리 기질부는 조면암질 조성을 보이며, 철 함량은 약 3 wt.%로 높은 편이다. 본 흑요암은 용암돔이나 칼데라의 붕괴에 의하여 고온상태에서 역-화산재 흐름 퇴적물이 계곡부로 운반된 후, 차가운 지표면과 접촉하였으며 유동성을 띠는 가운데서 급랭하여 형성된 것으로 보인다. The purpose of this study is to provide the information on genesis of obsidian occurring in the southwestern part of Ulleung Island, Korea, and to discuss its implications for volcanic activity through volcanological and mineralogical properties of obsidian. Obsidian occurs locally at the lower part of the Gombawi welded tuff, showing various complex textures and flow banding. Though obsidian is mostly homogeneous, it is closely associated with alkali feldspar phenocrysts, reddish tuff, and greyish trachyte fragments. The obsidian occurs as wavy, lenticular blocks or lamination composed of fragments. Cooling fractures developed on obsidian glass are characterized by perlitic cracks, orbicular or spherical cracks, indicating that obsidian rapidly quenched to form an amorphous silica-rich phase. It is evident that hydration took place preferentially at the outer rim relative to the core of obsidian, forming alteration rinds. The glassy matrix of obsidian includes euhedral alkali feldspars, diopside, biotite, ilmenite, and iron oxides. Microlites in glassy obsidian are composed mainly of alkali feldspars and ilmenite. Quantitative analysis by EPMA on the obsidian glass part shows trachytic composition with high iron content of 3 wt.%. Accordingly, obsidian formed with complex textures under a rapid cooling condition on surface ground, with slight rheomorphism. Such results might be induced by collapse of lava dome or caldera, which produced the block-and-ash flow deposit and the transportation into valley while keeping high temperatures.

Biodiversity hotspot for marine invertebrates around the Dokdo, East Sea, Korea: Ecological checklist revisited

We extensively reviewed the macrozoobenthos around Dokdo, Korea, by analyzing metadata collected over the past 50years. The Dokdo macrozoobenthos was represented by 578 species belonging to 243 families from 12 phyla, where four major phyla (or classes) collectively accounted for 86% to total. Mollusks, arthropods, and cnidarians were semi-equally occurred in intertidal and subtidal areas, while polychetes dominated the subtidal zone. The northern most region of Dokdo had the greatest biodiversity (173 species). The taxonomic distinctness analysis (delta+) indicated a close association between species by region, although the number of species varied greatly. About half of the species did not occur cross the locations (n=20), indicating strong habitat preferences of Dokdo macrofaunal assemblages. Overall, the diversity of Dokdo was greater than that of Ulleung Island (east coast), but comparable to that of the well-developed tidal flats in the western Korean waters, highlighting its status as a biodiversity hotspot.

Chloroplast DNA assessment of anagenetic speciation in Rubus takesimensis (Rosaceae) on Ulleung Island, Korea

Two major modes of speciation, i.e., cladogenesis and anagenesis, have often been used to explain the generation of distinct plant diversity on oceanic islands. Anagenetic speciation is of particular interest to plant endemics on Ulleung Island, Korea because, of nearly 40 endemics, approximately 88% or even all endemic species are thought to be anagenetically derived. Despite its important role in Ulleung Island, the full scope of the anagenetic speciation and the genetic consequences of this speciation mode have been limited to a very few families (i.e., Apiaceae, Sapindaceae, and Ranunculaceae). Based on extensive sampling (a total of 322 individuals) and trnL-F cpDNA noncoding sequences, we conducted phylogenetic and haplotype network analyses for the species pair of Rubus crataegifolius (continental progenitor)-R. takesimensis (insular derivative). Rubus takesimensis on Ulleung Island is not a monophyletic group, suggesting at least two independent introductions from geographically and genetically divergent source populations. No significant reduction in cpDNA haplotype diversity and genetic differentiation in R. takesimensis are consistent with expectations from the anagenetically derived species. A further study based on comprehensive sampling and more variable molecular markers will shed light into the genetic consequences of anagenetic speciation in Rubus on Ulleung Island.

Paenibacillus aceris sp. nov., isolated from the rhizosphere of Acer okamotoanum, a plant native to Ulleungdo Island, Republic of Korea.

Strain KUDC4121T was isolated from the rhizosphere of Acer okamotoanum, a plant native to the Korean island of Ulleungdo. The strain was a Gram-stain-positive, non-spore-forming, non-motile, rod-shaped bacterium that can grow at 18-37 °C and pH 6.0-7.5, with optimum growth at 30 °C and pH 7.0. It grew on tryptic soy agar containing less than 0.5 % (w/v) NaCl and in R2A broth. Cell length ranged from 2.0 to 2.5 µm. Strain KUDC4121T was oxidase- and catalase-positive and did not hydrolyse starch or casein. The genomic G+C content was 48.8 mol%. The major fatty acids were anteiso-C15 : 0 and iso-C16 : 0. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain KUDC4121T belongs to the genus Paenibacillus. The closest type strain was Paenibacillus chondroitinus DSM 5051T, with 97.8 % similarity, followed by Paenibacillus alginolyticus DSM 5050T (97.6 %), Paenibacillus ferrarius CY1T (97.5 %), Paenibacillus pocheonensis Gsoil 1138T (97.5 %), Paenibacillus frigoriresistens YIM 016T (97.5 %), Paenibacillus pectinilyticus RCB-08T (97.2 %) and Paenibacillus aestuarii CJ25T (96.9 %). Based on its phenotypic properties and phylogenetic and genetic data, strain KUDC4121T should be considered to represent a novel species of the genus Paenibacillus, for which the name Paenibacillus aceris sp. nov. is proposed. The type strain is KUDC4121T (=KCTC 13870T=DSM 24950T).

Laharz_py 프로그램을 이용한 울릉도 라하르 수치모의

화산지역에서 발생하는 라하르는 인명과 재산피해를 일으키는 주요 재해이다. 라하르 위험 지역을 산출하기 위하여 본 연구에서는 Laharz_py 프로그램을 사용하여 울릉도에서 발생 가능한 라하르의 영향 지역에 대한 수치모의를 실시하였다. 울릉도의 근위위험지역을 도출하기 위하여 DEM에 400 m의 추가고도를 설정하였고, 이에 따라 H/L비는 0.45와 0.5로 결정하였다. 발생 가능한 라하르의 부피는 30,000, 50,000, 70,000, <TEX>$100,000m^3$</TEX>로 설정하였다. 근위위험지역 경계와 인접하여 라하르의 발생 가능성이 있는 하천은 울릉도 동쪽 저동천, 남동쪽 사동천과 옥천천, 남서쪽의 남양천, 북쪽의 용출수인 추산천이며, 집중호우시 나리 분지에서의 라하르 발생 가능성도 고려하여 수치모의를 실시하였다. 본 연구는 울릉도에서 발생 가능한 라하르의 영향 범위에 대한 예측을 통해 화산재해로 인한 인명과 재산 피해를 줄일 수 있도록 재해위험지도를 작성하기 위한 기본 자료로 활용 가능할 것이다. A Lahar on the volcanic area is one of the important hazard that can cause the loss of life and property damage. In order to estimate lahar hazard area at Ulleung Island, we simulated lahar inundation area using Laharz_py. We assumed 400 m of additional elevation for DEM to draw proximal hazard zone of Ulleung Island that H/L ratio were selected 0.45 and 0.5. And lahar volumes for simulation were estimated to 30,000, 50,000, 70,000, <TEX>$100,000m^3$</TEX>, respectively. In the results, 5 streams are located near a proximal hazard zone, Jeodong (east), Sadong and Okchon (southeast), Namyang (southwest), and Chusan (north), Nari basin is also considered that has a possibility of lahar during downpour. The results of this study can be used as basic data to make a hazard map for reduce the damage that can be caused by volcanic hazards occurred on Ulleung Island.

Three New Recorded Species of the Physalacriaceae on Ulleung Island, Korea

Most known species in the Physalacriaceae are saprotrophs that grow on decaying leaves and wood, and approximately 21 genera in the Physalacriaceae have been reported worldwide. During an ongoing survey of indigenous fungi in Korea, four specimens belonging to the Physalacriaceae were collected on Ulleung Island. These specimens were identified as three species based on morphological characteristics and molecular analysis of rDNA-internal transcribed spacer sequences. Three species in three genera were shown to be new records in Korea: Hymenopellis orientalis, Paraxerula hongoi, and Ponticulomyces orientalis. The latter two are the first records of these genera in Korea. In this study, we provide detailed morphological descriptions of these species and describe their phylogenetic position within the Physalacriaceae.

Moderating effect of cognitive image in the relationship between risk perception and revisitation for visitors to Ulleung Island

Moderating effect of cognitive image in the relationship between risk perception and revisitation for visitors to Ulleung Island

Analytical Method for the Validation of Three Polyphenols as a Marker Compound for the Standardization of Solidago virgaurea subsp. gigantea Extracts and Antiadipogenesis of Harvesting Time and Location.

Protocatechuic acid (PC), chlorogenic acid (CA), and kaempferol-3-O-rutinoside (K-O-R), isolated from the Solidago virgaurea subsp. gigantea (SV) extract, were quickly and efficiently separated using HPLC. Our chromatographic method was found to effectively separate PC, CA, and K-O-R at retention times of 5.36, 8.22, and 17.04 min, respectively. Linearity of PC, CA, and K-O-R was found to be in the range of 4.85–485.00, 47.5–1900.00, and 8.50–850.00 μg/ml. Recoveries ranged between 101.32 and 103.30%, 95.82 and 100.25%, and 96.18 and 99.37%, for PC, CA, and K-O-R, respectively. The antiadipogenesis activity of SV extracts collected from five different months and from seven different regions was evaluated using an Oil Red O staining assay in 3T3-L1 cells. Extract from SV collected in April from the Ulleung Island produced over 106.89% inhibition of adipogenesis without cytotoxicity at 50 μg/ml. This extract had a high amount of PC and K-O-R. The developed HPLC method was found to be fast, accurate, precise, and reproducible and could be applied to qualitative and quantitative analysis of three bioactive compounds in SV extracts. The SV extract collected in April from Ulleung Island can be used as a functional food ingredient preventing obesity.

Volcanism and Petrogenesis of Dodong Basaltic Rocks in the Ulleung Island, East Sea

Volcanism and Petrogenesis of Dodong Basaltic Rocks in the Ulleung Island, East Sea

Occurrence and Forming Process of the Reddish Bed at Hwangto Cave, Ulleung Island, Korea

The Hwangto cave is a sea cave which is located near shore in the Taeha-ri, Ulleung Island, being composed of the reddish tuff wall rock, the topic of this study, and the trachyte ceiling rock. The chemical compositions of the red tuff layer are 49.81-63.63% of SiO2, 13.05-24.91% of Al2O3, 2.67-5.82% of Fe2O3, 2.87-6.92% of Na2O, 2.37-3.85% of K2O, 0.55-0.81% of TiO2, 0-0.53% of MnO, 0.39-1.75% of MgO, and 0.60-1.40% of CaO with a pH ranging from 4.5 to 8. The reddish tuff are composed of 23.7-39.4% of anorthoclase, 16.9-33.3% of sanidine, 15.8-26.1% of illite, 5.1-9.0% of hematite, 0-3.7% of goethite, 6.9-9.9% of titanium oxide, and 0.9-9.5% of halite in mineral composition. Although it only includes anorthoclase, sanidine, and illite as major minerals, there can be additional vitric minerals that could not detected by the XRD. The mineralogy and textures of the tuff layer indicate that it became reddish due to the formation of amorphous palagonite and the oxidation of the iron as a heat from the trachytic lava affects the underlying tuff to altered. This iron oxides are enriched in the palagonite, or form microcrystalline or amorphous minerals. We thus suggest that the red tuff layer was generated by the combination of the thermal oxidation involved in the trachytic lava flow on the tuff layer, the palagonitization of the matrix of the tuff, and the oxidation of iron-bearing minerals.

Physical boundaries of Intrathermocline Ulleung Eddies in the East/Japan Sea

A new eddy detection algorithm is developed based on the particle tracking experiment (PTE) to determine the spatial extent of the Intrathermocline Ulleung Eddies (IUE) in the East/Japan Sea (EJS). The new eddy detection method enables us to identify three regions of interests: the IUE, transitional regions between the IUE and mean currents, and mean current regions around IUE. The estimated spatial boundary of the IUE is expressed as least square fitted elliptic characteristics from February to September, 2015. Accordingly, we could obtain three main results after analyzing the IUE: (1) the center and the highest sea level height of the IUE are not co-located, suggesting that the IUE is sustained by potential vorticity through the relative vorticity in response to bathymetry changes; (2) the highest sea level is always anchored around Ulleung Island, which is associated with the relatively high Chlorophyll concentration (Chl) around the edge of the IUE; and (3) the shape of the IUE is strongly influenced by the current systems in the Ulleung Basin. Furthermore, the semi-major and semi-minor axes, and its ratios are determined as well as the mean areas and the highest sea surface height of the IUE to understand the spatio-temporal characteristics of the IUE and the dominant frequencies on it.

Phylogeny and Evolution of Endemic Species on Ulleungdo Island, Korea: The Case of &lt;I&gt;Fagus multinervis&lt;/I&gt; (Fagaceae)

Abstract The genus Fagus (beech, Fagaceae) consists of about 10 species discontinuously distributed in East Asia, eastern North America, and Europe. Fagus multinervis occurs only on Ulleungdo Island, Korea, and is an important component of the island's plant community. The species was described primarily based on one of its stem characteristics: it branches at the base, producing several primary trunks. In part because no extant Fagus species are found in mainland Korea, and because the closest populations of Fagus are found in Japan, F. multinervis has been considered to be derived from the Japanese species, F. japonica. However, a recent study synonymized F. multinervis under F. engleriana and proposed that the beech population of Ulleungdo Island was derived instead from China via long-distance dispersal. Fagus multinervis is morphologically very similar to F. engleriana, but it can be distinguished by having rhombic lenticels that are vertically elongated. We determined the nucleotide sequences of the...

Assessing Soil Fertility Status of Edible Wild Plants Fields in Ulleung Island

The perennial edible wild plants such as Aster glehnii, Solidago virgaurea subsp. gigantean, Allium ochotense, Athyrium acutipinnulum, Aruncus dioicus var. kamtschaticus and Codonopsis lanceolata have cultivated as the main income crops introduced into the fields about 30 years ago in Ulleung island. Soil samples were collected from 190 fields and assessed the effects of management practices on soil chemical properties at wild edible plant fields under no-till system. The strong acidic soils of pH 5.4 or less were detected in 45% of the soil samples. The level of soil organic matter was being held at mean 63±28 g kg -1 , 2.7 times higher than upland soils in Korea. Available phosphate and exchangeable potassium showed more than recommended levels of upland crops as 680±489 mg kg -1 and 1.94±1.7 cmolc kg -1 , respectively. The fields of Solidago and Aster showing strong soil acidity and high level of available phosphate and water soluble NO₃ - were distinguished from other crops in analysis of variance and principal component analysis of soil chemicals. These results suggested that high frequency of acidic soil and high levels of available P₂O 5 , exchangeable K₂O and water soluble NO₃ - were accompanied with the use of urea and NPK-fertilizer based on nitrogen in the field. However, further research is needed to understand the appropriate management of fertilization and the prevention of soil acidification for wild edible plants.