Typical Structure Research Articles

Comparative Analysis of Six Chloroplast Genomes in Chenopodium and Its Related Genera (Amaranthaceae): New Insights into Phylogenetic Relationships and the Development of Species-Specific Molecular Markers.

Species within the genus Chenopodium hold significant research interest due to their nutritional richness and salt tolerance. However, the morphological similarities among closely related species and a dearth of genomic resources have impeded their comprehensive study and utilization. In the present research, we conduct the sequencing and assembly of chloroplast (cp) genomes from six Chenopodium and related species, five of which were sequenced for the first time. These genomes ranged in length from 151,850 to 152,215 base pairs, showcased typical quadripartite structures, and encoded 85 protein-coding genes (PCGs), 1 pseudogene, 37 tRNA genes, and 8 rRNA genes. Compared with the previously published sequences of related species, these cp genomes are relatively conservative, but there are also some interspecific differences, such as inversion and IR region contraction. We discerned 929 simple sequence repeats (SSRs) and a series of highly variable regions across 16 related species, predominantly situated in the intergenic spacer (IGS) region and introns. The phylogenetic evaluations revealed that Chenopodium is more closely related to genera such as Atriplex, Beta, Dysphania, and Oxybase than to other members of the Amaranthaceae family. These lineages shared a common ancestor approximately 60.80 million years ago, after which they diverged into distinct genera. Based on InDels and SNPs between species, we designed 12 pairs of primers for species identification, and experiments confirmed that they could completely distinguish 10 related species.

Fractional-order modelling and dynamic analysis on a typical beam structure incorporated with a semi-active particle damping equipment

Semi-active particle damping equipments exhibit considerable adaptability when implemented in flexible structures. However, their dynamic characteristics become complex due to the presence of a substantial quantity of particles within the damping equipment, and the influence of the internal magnetic field necessitates careful consideration. The fractional-order model of this kind of equipment is established, wherein the designed parameters kp and α are respectively associated with the filling ratio and the input current. Depending on the Hertz-Mindlin contact model, the multi-body dynamic issues inner the damping equipment are addressed using the Discrete Element Method, and then the fractional-order parameters kp and α are identified based on the energy distribution observed within a single vibration period. The numerical simulations, conducted using the fractional-order model, show a fundamental consistency with the experimental results. This alignment serves to validate the accuracy and feasibility of the fractional-order model. Finally, the fractional-order model is utilized to investigate the optimized value of the input current and to analyze the motion trajectory of the damping equipment under various scenarios. The results indicate that the optimized input current is approximately 2.5 A; and as the parameter kp and α increase, the motion trajectory of the system will rotate in a counterclockwise direction, accompanied by a gradual outward displacement of the outermost curve relative to the origin position.

Comparative and phylogenetic analysis of Chiloschista (Orchidaceae) species and DNA barcoding investigation based on plastid genomes

BackgroundChiloschista (Orchidaceae, Aeridinae) is an epiphytic leafless orchid that is mainly distributed in tropical or subtropical forest canopies. This rare and threatened orchid lacks molecular resources for phylogenetic and barcoding analysis. Therefore, we sequenced and assembled seven complete plastomes of Chiloschista to analyse the plastome characteristics and phylogenetic relationships and conduct a barcoding investigation.ResultsWe are the first to publish seven Chiloschista plastomes, which possessed the typical quadripartite structure and ranged from 143,233 bp to 145,463 bp in size. The plastomes all contained 120 genes, consisting of 74 protein-coding genes, 38 tRNA genes and eight rRNA genes. The ndh genes were pseudogenes or lost in the genus, and the genes petG and psbF were under positive selection. The seven Chiloschista plastomes displayed stable plastome structures with no large inversions or rearrangements. A total of 14 small inversions (SIs) were identified in the seven Chiloschista plastomes but were all similar within the genus. Six noncoding mutational hotspots (trnNGUU–rpl32 > rpoB–trnCGCA > psbK–psbI > psaC–rps15 > trnEUUC–trnTGGU > accD–psaI) and five coding sequences (ycf1 > rps15 > matK > psbK > ccsA) were selected as potential barcodes based on nucleotide diversity and species discrimination analysis, which suggested that the potential barcode ycf1 was most suitable for species discrimination. A total of 47–56 SSRs and 11–14 long repeats (> 20 bp) were identified in Chiloschista plastomes, and they were mostly located in the large single copy intergenic region. Phylogenetic analysis indicated that Chiloschista was monophyletic. It was clustered with Phalaenopsis and formed the basic clade of the subtribe Aeridinae with a moderate support value. The results also showed that seven Chiloschista species were divided into three major clades with full support.ConclusionThis study was the first to analyse the plastome characteristics of the genus Chiloschista in Orchidaceae, and the results showed that Chiloschista plastomes have conserved plastome structures. Based on the plastome hotspots of nucleotide diversity, several genes and noncoding regions are suitable for phylogenetic and population studies. Chiloschista may provide an ideal system to investigate the dynamics of plastome evolution and DNA barcoding investigation for orchid studies.

Research on the Hybrid Wind–Solar–Energy Storage AC/DC Microgrid System and Its Stability during Smooth State Transitions

The hybrid AC/DC microgrid is an independent and controllable energy system that connects various types of distributed power sources, energy storage, and loads. It offers advantages such as a high power quality, flexibility, and cost effectiveness. The operation states of the microgrid primarily include grid-connected and islanded modes. The smooth switching between these two states is a key technology for ensuring the flexible and efficient operation of the microgrid. In this paper, the typical structure of an AC–DC hybrid microgrid and its coordination control strategy are introduced, and an improved microgrid model is proposed. Secondly, an adaptive current–voltage–frequency integrated control method based on signal compensation is proposed to solve the impulse current and voltage generated during the switching between a grid-connected state and an off-grid state. Finally, in response to unplanned grid-connected scenarios, an improved pre-synchronization control strategy based on BP neural networks is introduced to rapidly restore stable operation. The proposed control strategies enhanced the steady-state and transient stability of the hybrid wind–solar–energy storage AC/DC microgrid, achieving seamless grid-connected and islanded transitions without disturbances. The simulation and experimental results validated the correctness and effectiveness of the proposed theories.

Conceptual Propositions for Creation of Perspective Robotic Systems based on Platform-Modular Approach

Intensive introduction of robotic systems is a modern priority for further automation of human activities. Recent theoretical and practical developments in robotics have made it possible to introduce robots in areas of practical activities previously dominated by humans. Modern trend in robotics is in creating state of the art robotic systems with increased autonomy and expanded functionality. This will allow to relieve human, leaving him supervision functions. An emerging task in robotics is also to create an environment, assisting to create and introduce new perspective robotic systems, also bearing modernization capability. This can be done through improving of modern approaches of creating robotic systems. We foresee necessity to change some of robots’ life-cycle stages, which would allow to rapidly introduce new effective robots into production. The article in its beginning studies some most emerging directions in robotics and new ideas for more effective robotic systems design. During this one should find a balance between introducing drastically new technologies in new robot and perfectioning already existing technologies. Authors propose to use so-called modular-platform based approach for creating new robots. Within it they imply typical structure of a robot, suggesting to use basic platform as a basement for building new robots with varying usefull load. In such case same platform can be used for building inspection robots, unmanned transport systems, unmanned retransmitter etc. The paper presents some already built examples of the approach. Final part of the paper discusses advantages given by application of this approach.

The Characterization of Mitochondrial Genome of Spotted Pond Turtle (Geoclemys hamiltonii).

The spotted pond turtle Geoclemys hamiltonii (Gray, 1830) is widely distributed in the Indus, Ganges, and Brahmaputra river basins. In this study, the complete mitochondrial genome (mitogenome) of G. hamiltonii was sequenced using the next-generation sequencing (NGS) and Sanger sequencing, and the essential characteristics, gene arrangement, and phylogenetic relationship were analyzed. The results showed that the G. hamiltonii mitogenome was 16,505bp in length (A: 33.6%, C: 27.1%, G: 13.4%, T: 25.8%) and consisted of 22 tRNAs, 13 protein-coding genes, two ribosomal RNA genes, and a non-coding control region (GenBank accession ON243873). The genome composition of G. hamiltonii presented a slight A + T bias (59.4%), and showed a positive AT skew (0.131) and a negative GC skew (-0.338). All tRNAs had the typical clover structure, except trnS1 (GCT). The gene order of the G. hamiltonii mitogenome was the same as other Geoemydidae mitogenomes. A phylogenetic analysis based on the complete mitogenome indicated that the G. hamiltonii grouped independently of other species in the family Geoemydidae, supporting the species' placement in the monotypic genus Geoclemys. Our results describe a novel genome at the species level. As the first complete mitogenome of G. hamiltonii, it provided valuable molecular information for phylogenetic and conservation genetics analyses of G. hamiltonii.

Enhanced strength-ductility synergy of Al/Zn/Al laminated composite with unique interface and multi-modal heterogeneous microstructure

Laminated bimetal composites have become an important strategy for designing and manufacturing desirable metallic components. Here, Al/Zn/Al laminated composite was fabricated by porthole die co-extrusion (PDCE) process, offering excellent mechanical performance. An obvious transition zone with a dispersed duplex structure (α and η phases) instead of intermetallic compounds was formed in Al/Zn interface due to element inter-diffusion and eutectoid transformation. The α/η phase interface showed a 3D characteristic with compositional and structural gradients. Moreover, Al/Zn/Al composite displayed a multi-modal heterogenous structure including the typical macroscopic hierarchical structure by alternately stacking Al and Zn layers with different microstructures, the gradient structure from Al/Zn interface to the center of Al or Zn layer, and the bimodal structure formed either near Al/Zn interface or in the layer center. As a result, Al/Zn/Al composite exhibits superior mechanical performance compared with the individual component (Al or Zn) and a satisfactory balance between strength and ductility.

Colloid Pattern of Salivary Mucinous Adenocarcinomas With Recurrent BRAF V600E Mutations.

The relationship between various patterns of mucin-producing salivary adenocarcinomas, including invasive salivary adenocarcinomas with mucinous differentiation, such as colloid and papillary carcinomas, remains unclear. Herein, we aimed to describe the clinicopathologic characteristics, immunophenotypes, molecular underpinnings, and clinical behavior of salivary mucinous adenocarcinomas (MA) to clarify their classification. We described a broad series of colloid and papillary patterns of MAs, indicating that papillary pattern presented papillary cystic proliferation of mucinous columnar cells as salivary intraductal papillary mucinous neoplasms with recurrent AKT1 E17K mutations, whereas colloid adenocarcinomas containing large mucinous pools or lakes around the malignant epithelial nests or islands harbored BRAF V600E mutations with worse prognosis. Typical morphologic structures, CK7(+), CK20(-), CDX2(-), p63(-), p40(-), MAML2 fluorescence in situ hybridization (-), AR(-), TTF-1(-), S100(-), mammaglobin(-), or S100/mammaglobin(+) with ETV6 fluorescence in situ hybridization (-) immunophenotype, and recurrent AKT1 E17K or BRAF V600E mutations may be defined. To our knowledge, this small series represents the first genetic study on a typical colloid pattern of MA, and our study with the spectrum documentation for MA in clinicopathologic characteristics, histologic and immunophenotypes, molecular features, and clinical behavior will allow for a better understanding of these rare but distinctive tumors.

Complete chloroplast genome sequences of the ornamental plant Prunus cistena and comparative and phylogenetic analyses with its closely related species

BackgroundPrunus cistena is an excellent color leaf configuration tree for urban landscaping in the world, which has purplish red leaves, light pink flowers, plant shape and high ornamental value. Genomic resources for P. cistena are scarce, and a clear phylogenetic and evolutionary history for this species has yet to be elucidated. Here, we sequenced and analyzed the complete chloroplast genome of P. cistena and compared it with related species of the genus Prunus based on the chloroplast genome.ResultsThe complete chloroplast genome of P. cistena is a 157,935 bp long typical tetrad structure, with an overall GC content of 36.72% and higher GC content in the in the inverted repeats (IR) regions than in the large single-copy (LSC) and small single-copy (SSC) regions. It contains 130 genes, including 85 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The ycf3 and clpP genes have two introns, with the longest intron in the trnK-UUU gene in the LSC region. Moreover, the genome has a total of 253SSRs, with the mononucleotide SSRs being the most abundant. The chloroplast sequences and gene arrangements of P. cistena are highly conserved, with the overall structure and gene order similar to other Prunus species. The atpE, ccsA, petA, rps8, and matK genes have undergone significant positive selection in Prunus species. P. cistena has a close evolutionary relationship with P. jamasakura. The coding and IR regions are more conserved than the noncoding regions, and the chloroplast DNA sequences are highly conserved throughout the genus Prunus.ConclusionsThe current genomic datasets provide valuable information for further species identification, evolution, and phylogenetic research of the genus Prunus.

Formation mechanisms and control strategies of metallurgical defects and microstructures during laser powder bed fusion of AlSi10Mg lattice structures

Lattice structures fabricated using laser powder bed fusion (LPBF) are pivotal for lightweight design. However, their small dimensions and intricate geometry intensify the likelihood of defect formation, such as lack of fusion (LOF), near-spherical pores, and microstructural coarsening. In this study, it was observed that the central region of lattice struts was especially susceptible to large-sized LOF in conjunction with the concave top surfaces due to frequent laser jumps. Furthermore, overheating during turnaround was identified as a contributor to excessive pores and surface bulging. Intriguingly, a unique layered melt mode emerged, characterized by coarse columnar grains exceeding 30 μm in size. A novel approach is proposed to reduce defects, refine grains, and enhance hardness during LPBF process, utilizing low laser power and a continuous scanning strategy. Using this manipulation strategy on typical lattice structures, porosity levels decreased by over 40 %. Ultimately, this research offers invaluable insights into refining printing conditions for lattice structures.

Comparative and phylogenetic analyses of nine complete chloroplast genomes of Orchidaceae

The orchid family has 200,000 species and 700 genera, and it is found worldwide in the tropics and subtropics. In China, there are 1247 species and subspecies of orchids belonging to the Orchidaceae family. Orchidaceae is one of the most diverse plant families in the world, known for their lush look, remarkable ecological tolerance, and capability for reproduction. It has significant decorative and therapeutic value. In terms of evolution, the orchid family is one of the more complicated groups, but up until now, little has been known about its affinities. This study examined the properties of 19 chloroplast (cp) genomes, of which 11 had previously been published and nine had only recently been revealed. Following that, topics such as analysis of selection pressure, codon usage, amino acid frequencies, repeated sequences, and reverse repeat contraction and expansion are covered. The Orchidaceae share similar cp chromosomal characteristics, and we have conducted a preliminary analysis of their evolutionary connections. The cp genome of this family has a typical tepartite structure and a high degree of consistency across species. Platanthera urceolata with more tandem repeats of the cp genome. Similar cp chromosomal traits can be seen in the orchidaceae. Galearis roborowskyi, Neottianthe cucullata, Neottianthe monophylla, Platanthera urceolata and Ponerorchis compacta are the closest cousins, according to phylogenetic study.

Characterization of the complete chloroplast genome assembly of Amorphophallus yunnanensis Engler, Pflanzenr (Araceae) from southwestern China

Southwestern China is a biodiversity hotspot due to its diverse topography and environment. Amorphophallus yunnanensis is a species of perennial herb that is mainly distributed throughout southwestern China. The genetic diversity and divergence in this species have not been assessed largely due to a lack of genomic resources. To help with the phylogeographic study, we sequenced and assembled the first complete chloroplast genome sequence of A. yunnanensis. The length of the chloroplast genome was 164,417 bp, with an average GC content of 36% (GenBank accession no. OR400247). The genome possessed a typical quadripartite structure, and the lengths of the large single-copy (LSC), small single-copy (SSC), and two inverted repeat (IR) regions were 92,149 bp, 15,182 bp, 28,543bp, and 28,543bp, respectively. A total of 128 genes were annotated across the genome, including 82 protein-coding genes, 8 rRNAs, and 38 tRNAs. The maximum likelihood (ML) phylogeny confirmed the phylogenetic position of Amorphophallus within Araceae, with the Amorphophallus species forming a single monophyletic clade with a high bootstrap value. The ML tree also indicated that A. yunnanensis was most closely related to A. coaetaneus. This newly sequenced chloroplast genome assembly will aid in future studies of genetic diversity, historical population dynamics, and geographic differentiation patterns of A. yunnanensis.

The complete chloroplast genome assembly of Amorphophallus krausei Engler, Pflanzenr 1911 (Araceae) from southwestern China

Plants in the genus Amorphophallus, many of which possess high konjac glucomannan content, are considered important cash crops in many Asian countries. Wild relatives of cultivated Amorphophallus species are valuable resources for the genetic improvement of these crops. To aid in future genetic research of wild germplasm resources of Amorphophallus, a single individual of Amorphophallus krausei Engler, Pflanzenr 1911 was collected from southwestern China, and its chloroplast genome was sequenced using next-generation sequencing technologies. The assembled chloroplast genome was 172,418 bp in length with a GC content of 35.23% (GenBank accession no. OR416863). A typical quadripartite structure was found in the genome, which was comprised of one large single-copy (LSC), one small single-copy (SSC), and two inverted repeats (IRs), with lengths of 91,983 bp, 15,591 bp, 32,422 bp, and 32,422 bp, respectively. A total of 132 genes were annotated in the genome, including 86 protein-coding genes, 38 tRNAs, and 8 rRNAs. A maximum likelihood (ML) tree of A. krausei and 17 other species in the family Araceae suggested that all Amorphophallus species formed a single monophyletic clade. A close relationship among A. konjac, A. albus, and A. krausei was also revealed by the phylogenetic tree. The newly sequenced chloroplast genome of A. krausei will support future genetic studies, particularly the assessment of genetic diversity, resource conservation, and phylogeographic research.

Characterization of chloroplast genome and phylogenetic analysis of the Elaeagnus hybrid variety E. ‘viridis’ (Elaeagnaceae)

Elaeagnus ‘viridis’, an artificial hybrid of E. macrophylla (♂) Thunb. (1784) × E. pungens (♀) Thunb. (1784), is known for its economic and ecological value. In this study, we sequenced and assembled the whole chloroplast (cp) genome of E. ‘viridis’. The results showed that its cp genome was 152,284 bp long, showing a typical quadripartite structure and containing a large single-copy region (LSC, 82,299 bp), a small single-copy region (SSC, 18,239 bp), and a pair of inverted repeats (IRs, 51,746 bp). The cp genome contains 132 genes, including 86 protein-coding genes (PCGs), 38 tRNA genes, and 8 rRNA genes. Phylogenetic analysis based on 66 common PCGs revealed that E. ‘viridis’ is most closely related to its maternal parent E. pungens. The chloroplast genomic information reported in this study will shed some useful light for further genetic studies in the genus Elaeagnus.

The complete chloroplast genome of Porella gracillima Mitt. (Porellaceae) and phylogenetic relationships to other bryophytes

Porella gracillima Mitt. (Jungermanniidae, Porellaceae), a bryophyte is widespread in temperate Asia and North America. In Korea, P. gracillima is mainly observed in shaded and dried rocks or tree trunks on mountains. Here, we determined the complete chloroplast (cp) genome sequence of P. gracillima to provide useful genetic information in the phylogenetic relationship, phylogeographic history, and conservation of the species. The complete cp genome of P. gracillima was assembled using NGS Illumina HiSeqX platform. The cp genome was 121,867 bp in length (GC contents, 33.7%) and showed a typical quadripartite structure, consisting of a large single copy (LSC) of 83,406 bp, a small single copy (SSC) of 19,692 bp, and two inverted repeats (IRs) of 9,385 bp. Phylogenetic analysis shows that Porellaceae was a sister group of Radulaceae, which agrees with the findings of the previous phylogenetic studies. Our cp genome data of P. gracillima may contribute to a better understanding of the evolution of the Porella in Porellaceae and will help to infer its molecular identification, thereby providing a guideline for conservation.

The complete chloroplast genome of Spergularia marina (Caryophyllaceae) and its phylogenetic analysis

Spergularia marina (L.) Griseb, 1843 (Caryophyllaceae) is a halophytic plant widely distributed along the southwestern coast of the Korean Peninsula. In this study, the complete chloroplast genome sequence of S. marina was determined using next-generation sequencing (NGS). The chloroplast genome of S. marina is 152,460 bp in length with 36.7% GC content. It comprises a large single-copy (LSC; 83,321), a small single copy (SSC; 17,205 bp), and a pair of inverted repeats (IRs; 25,967 bp) with a typical quadripartite structure. It consists of 131 genes, including 86 protein-coding genes, 8 ribosomal RNAs, and 37 transfer RNAs. Phylogenetic analysis using complete chloroplast genomes showed that among the 17 Caryophyllaceae species, S. marina is most closely related to Spergula arvensis. Since no complete chloroplast genome of the genus Spergularia has been reported to date, our study provides useful genetic information for determining phylogenetic relationships within the Caryophyllaceae.

Experimental investigation on biaxial mechanical properties of laminated rock and floor heave mechanism of tunnel based on DIC

Floor heave at the bottom of a tunnel is a common defect during tunnel service. To study the deformation behavior and failure characteristics of the floor, laminated sandstone specimens with precracked U-shaped grooves were prepared, and a series of biaxial compression tests were carried out in the laboratory. Two typical floor structures of a tunnel (with and without inverts) and four lateral stress levels (σ2 = 0, 5, 10 and 15 MPa) were considered. The effects of lateral stress and inverted structure on the deformation and failure characteristics of the floor were investigated. The displacement, strain evolution and failure extent of the floor were analyzed based on the digital image correlation (DIC) technique. The testing results show that the stress concentration at the U-shaped grooves of the specimens can be effectively reduced by the inverted structure. The specimen with an invert can bear more axial deformation and axial stress than that without an invert. The V-shaped notch failure behavior occurred near the tunnel sidewalls for the specimen without an invert, and it occurred in the middle of the tunnel floor for the specimen with an invert. The failure intensity of the V-shaped notch was positively related to the lateral stresses. With the increasing of axial stress, the heave displacement at the tunnel floor gradually increased, while the settlement displacement of the sidewall showed a trend of first increasing and then decreasing. The tensile strain concentration area of the floor undergoes a process of increasing and then decreasing and finally transforms to compressive strain. The failure characteristics of the sandstone specimen observed in the laboratory tests are consistent with the phenomena in real tunnel engineering. In addition, the deformation and failure mechanism of the tunnel floor was analyzed. The key points for the treatment of tunnel floor heave are put forward based on the testing results.

Bacteriophage Engineering for Improved Copper Ion Binding.

In this study, fd viruses are genetically modified to display seven cropped versions (H, HG, HGF, HGFA, HGFAN, HGFANV and HGFANVA) of the previously identified Cu(II) specific peptide (HGFANVA). Atomic force microscopy (AFM) imaging reveals the typical filamentous structures of recombinant phages with thicknesses of ≈2-5nm in dry state. Scanning electron microscopy (SEM) imaging shows that HGFANVA viruses form larger elongated assemblies than H viruses that are deposited with a mineral layer after Cu(II) treatment. C and N peaks are detected for virus samples through Energy dispersive X-ray spectroscopy (EDX) analyses confirming the presence of phage organic material. Cu peak is only detected for engineered viruses after Cu(II) exposure. Enzyme-linked immunosorbent assay (ELISA) analyses show the selective Cu(II) binding of engineered phages. Agarose gel electrophoresis (AGE) and zeta potential analyses reveal negative surface charges of engineered viral constructs. Positively charged Cytopore beads are coated with bacteriophages and used for Cu(II) ion sorption studies. ICP-MS analyses clearly show the improved Cu(II) binding of engineered viruses with respect to wild-type fd phages. Such bottom-up constructed, genetically engineered virus-based biomaterials may be applied in bioremediation studies targeting metal species from environmental samples.

The complete chloroplast genome of Ziziphus mairei Dode 1908 (Rhamnaceae), an endangered perennial plant in Yunnan, China

Ziziphus mairei Dode 1908 (Rhamnaceae) is a rare and endangered perennial plant in Yunnan, China. In this study, we sequenced, assembled, and annotated the complete chloroplast genome of Z. mairei. The complete chloroplast genome was a closed circular molecule of 161,546 bp with a typical tetrad structure, containing a large single-copy (LSC) region of 89,252 bp, a small single-copy (SSC) region of 19,364 bp, and a pair of inverted repeat (IR) regions of 26,465 bp. A total of 128 genes have been annotated, including 83 protein-coding genes, 37 tRNA genes, and 8 rRNA genes. The GC content is 36.7%. Phylogenetic analysis revealed that Z. mairei is closely related to Z. hajarensis, Z. jujuba, and Z. jujuba var. spinosa. Our results provide useful genetic resources for further studies on the conservation and evolution of Z. mairei.

The complete chloroplast genome of Goodyera yunnanensis Schltr.

The family Orchidaceae is renowned for its extensive diversity. Within this family, the genus Goodyera R. Br. is classified under the subtribe Goodyerinae, comprising approximately 99 species. In this study, a species Goodyera yunnanensis Schltr., its plastid genome was characterized. The plastid genome of G. yunnanensis is 146,197 bp in size and exhibits a typical quadripartite structure with a pair of inverted repeat regions (IRs) of 25,611 bp, a large single-copy region (LSC) of 81,300 bp and a small single-copy region (SSC) of 13,675 bp. A total of 126 genes were identified, containing 80 protein-coding genes, 38 tRNA genes and 8 rRNA genes. The overall GC content is 37.2%, with corresponding values of 43.3%, 34.7% and 29.1% in IR, LSC and SSC regions, respectively. Forty-seven simple sequence repeats (SSRs) are found in G. yunnanensis plastome, and the frequency of mononucleotide repeats is significantly higher than other repeat types. Phylogenetic analysis indicates that Goodyera is resolved into four clades. G. yunnanensis belongs to the monophyletic clade A, and its phylogenetic position can be reasonably supported by morphological and molecular data.