Typical Profile Research Articles

Altered expression of the Plexin-B2 system in tuberous sclerosis complex and focal cortical dysplasia IIb lesions.

Tuberous sclerosis complex (TSC) and focal cortical dysplasia (FCD) type IIb are the predominant causes of drug-refractory epilepsy in children. Dysmorphic neurons (DNs), giant cells (GCs), and balloon cells (BCs) are the most typical pathogenic profiles in cortical lesions of TSC and FCD IIb patients. However, mechanisms underlying the pathological processes of TSC and FCD IIb remain obscure. The Plexin-B2-Sema4C signalling pathway plays critical roles in neuronal morphogenesis and corticogenesis during the development of the central nervous system. However, the role of the Plexin-B2 system in the pathogenic process of TSC and FCD IIb has not been identified. In the present study, we investigated the expression and cell distribution characteristics of Plexin-B2 and Sema4C in TSC and FCD IIb lesions with molecular technologies. Our results showed that the mRNA and protein levels of Plexin-B2 expression were significantly increased both in TSC and FCD IIb lesions versus that in the control cortex. Notably, Plexin-B2 was also predominantly observed in GCs in TSC epileptic lesions and BCs in FCD IIb lesions. In contrast, the expression of Sema4C, the ligand of Plexin-B2, was significantly decreased in DNs, GCs, and BCs in TSC and FCD IIb epileptic lesions. Additionally, Plexin-B2 and Sema4C were expressed in astrocytes and microglia cells in TSC and FCD IIb lesions. Furthermore, the expression of Plexin-B2 was positively correlated with seizure frequency in TSC and FCD IIb patients. In conclusion, our results showed the Plexin-B2-Sema4C system was abnormally expressed in cortical lesions of TSC and FCD IIb patients, signifying that the Plexin-B2-Sema4C system may play a role in the pathogenic development of TSC and FCD IIb.

Atypicality index as an add-on to combined first-trimester screening for chromosomal aberrations.

To compute a set of atypicality indices based on combined first-trimester screening (cFTS) markers and second-trimester estimated fetal weight (EFW), and to demonstrate their potential in identifying pregnancies at reduced or increased risk of chromosomal aberrations following a low-risk cFTS result. The atypicality index quantifies the unusualness of an individual set of measurements relative to a reference distribution and can be computed from any variables or measurements available. A score of 0% on the atypicality index represents the most typical profiles, while a score of 100% indicates the highest level of atypicality. From the Danish Fetal Medicine Database, we retrieved data on all pregnant women seen for cFTS in the Central Denmark Region between January 2008 and December 2018. All pregnancies with a cytogenetic or molecular analysis obtained prenatally, postnatally or following pregnancy loss or termination were identified. A first-trimester atypicality index (AcFTS) was computed based on nuchal translucency (NT) thickness, maternal serum free β-human chorionic gonadotropin (β-hCG) and pregnancy-associated plasma protein-A (PAPP-A). Furthermore, a second-trimester index (AcFTS + EFW) was computed from cFTS markers and EFW from a routine second-trimester anomaly scan. All pregnancies were stratified into subgroups based on their atypicality levels and their cFTS risk estimates. The risk of chromosomal aberrations in each subgroup was then compared with the overall prevalence, and a graphical presentation of the multivariate measurement profiles was developed. We retrieved data on 145 955 singleton pregnancies, of which 9824 (6.7%) were genetically examined. Overall, 1 in 122 (0.82% (95% CI, 0.77-0.87%)) of all pregnancies seen for cFTS were affected by a fetal chromosomal aberration, and in screen-negative pregnancies (cFTS trisomy 21 risk < 1 in 100 and/or trisomy 18/13 risk < 1 in 50), 0.41% (95% CI, 0.38-0.44%) were affected. In screen-negative pregnancies with a typical first-trimester profile (AcFTS < 80%), the risk of chromosomal aberrations was significantly reduced (0.28%) compared with the overall risk. The risk of chromosomal aberrations increased with higher atypicality index to 0.49% (AcFTS [80-90%)), 1.52% (AcFTS [90-99%)) and 4.44% (AcFTS ≥ 99%) and was significantly increased in the two most atypical subgroups. The same applied for the second-trimester atypicality index, with risks of chromosomal aberrations of 0.76% and 4.16% in the two most atypical subgroups (AcFTS + EFW [90-99%) and AcFTS + EFW ≥ 99%, respectively). As an add-on to cFTS, the atypicality index identifies women with typical measurement profiles, which may provide reassurance, whereas atypical profiles may warrant specialist referral and further investigation. In pregnancies identified as low risk on cFTS but with a highly atypical distribution of NT, PAPP-A and β-hCG, the risk of a chromosomal aberration is substantially increased. The atypicality index optimizes the interpretation of pre-existing prenatal screening profiles and is not limited to cFTS markers or EFW. © 2023 International Society of Ultrasound in Obstetrics and Gynecology.

A Flexible, Multi-Instrument Optimal Estimation Retrieval for Wind Profiles

Abstract Instruments such as Doppler lidars, radar wind profilers, and uncrewed aircraft systems could be used in observation networks to fill in the temporal and spatial gap that exists for low-level wind observations. These instruments, however, do not directly observe the wind and require a retrieval to be used to obtain wind estimates from their observations. Also, the depth and uncertainty of observations collected by these instruments vary depending on the environment that they are sampling. Optimal estimation is a variational retrieval method that combines information from a prior dataset and observations to retrieve an atmospheric state. This technique can be beneficial to use when observations have large uncertainties or provide insufficient information to obtain the atmospheric state by themselves. A new optimal estimation retrieval for obtaining wind profiles from typical lower atmospheric wind profiling instrumentation has been developed. This retrieval allows for more observations from wind profiling instrumentation to be used when retrieving wind profiles, increases the depth of retrieved profiles, and eliminates vertical data gaps. This retrieval can also be used to easily combine observations from different instruments or even with model data to create combined data wind retrievals that leverage the strengths of the different data sources to retrieve a wind profile that is superior to those obtained by the individual observations or data sources. It is envisioned that this retrieval will be continued to be developed and maintained as community software as lower atmospheric wind observing capabilities further develop and expand.

Ionospheric responses to solar flares: An analysis of ion composition changes during Moderately High Solar Activity in January 2023

This set of reports analyzes the ionospheric composition and effects of solar flares in January 2023 during periods of moderate to high solar activity. On January 6th, the solar zenith angle was 109.5 degrees, solar radio flux F10.7 was 119.2, and sunspot number was 70.2. On January 9th, the solar zenith angle reached 166.9 degrees, F10.7 was 119.4, and sunspot number was 70.4. On January 10th, the solar zenith angle was 137.1 degrees, F10.7 measured 119.4, and sunspot number remained at 70.4. The ionospheric data reveals typical profiles dominated by molecular ions at lower altitudes and lighter atomic ions higher up. An O+ layer peaked around 180 km. Comparison to quiet conditions shows impacts from the M-class and X-class flares on these days, including an enhanced and broadened O+ layer, increased molecular ions at 400-600 km, and temporary depletions of lighter ions around 500 km. These ionospheric changes match the flare timing and can be explained by flare-driven reactions. Geomagnetic activity also increased, signaling solar-terrestrial coupling. Together, the data provides evidence of complex atmospheric changes from solar flares spanning the electromagnetic spectrum during periods of moderately high solar activity. Analysis of ion composition gives insights into fundamental ionospheric processes and improving space weather prediction capabilities.

Abstract B091: Mechanisms underlying the omental support of ovarian cancer peritoneal metastasis

Abstract More than 80% of ovarian cancer (OC) cases have metastasized to the peritoneal cavity at diagnosis. The five-year survival rate for these patients is 25%. Within the peritoneal cavity, the most common and early site of metastasis is the omentum, a well-vascularized, specialized adipose tissue that arises off the greater curvature of the stomach. Because OC homes to the omentum, it is removed as a standard of care for patients with peritoneal metastases. In mouse models, the omentum is also an early metastatic site, and omentectomy reduces tumor expansion. The mechanism by which the omentum promotes OC growth is unknown, perhaps due to the complex omental microenvironment. The omentum contains many features that could impact OC seeding. It is covered by a mesothelial lining that extends in a sheet anchoring the omentum to surrounding tissues and is highly perfused by blood vessels. It contains lymphoid aggregates or milky spots that may contribute to pro- and anti-tumor immunity. This study explores the association of OC metastases with specific omentum features. We intraperitoneally injected luciferase-expressing ID8 OC cells to mimic high-grade serous OC metastasis. Using high-resolution, spatial ex vivo bioluminescence imaging (BLI), we found that tumor nodules preferentially formed in adipocyte-rich regions of the omentum and the mesentery, another peritoneal tissue favorable for OC seeding. To determine whether adipocytes, the most abundant omental cell type, are the primary support of OC, we crossed Adiponectin Cre+ and DTA mice to generate mice lacking white and brown adipocytes from birth. To overcome the metabolic syndrome the fatless mice develop, they receive subcutaneous fat transplants dorsally, referred to as distal adipocyte rescue of lipodystrophy (DARL) mice. Littermate controls receive sham surgeries. The blood triglyceride, glucose, and insulin levels are normalized post-transplant. Thus, the peritoneal cavity lacks mature adipocytes, but the mice have typical metabolic profiles, allowing us to study the role of omental adipocytes in peritoneal metastasis. Once the mice recovered from surgery, they were intraperitoneally injected with luciferase-expressing ID8s. While it was hypothesized that OC metastatic seeding within the adipocyte-free omentum and associated peritoneum would be impaired without local mature adipocytes, BLI revealed that the tumor burden was not decreased in DARL mice. Notably, specific regions of OC seeding, where mature adipocytes would have localized if present, such as the omentum and mesentery, retained tumor nodules in DARL mice. This conserved OC localization suggests that other cells in this specific microenvironment might be responsible for metastatic spread. To identify these cells, studies are ongoing to profile these regions in the DARL and control mice via flow cytometry and single-cell RNA sequencing. By unraveling the support for OC cells in the omentum, therapeutic strategies can be identified to repolarize the metastatic milieu and halt further spread to improve patient outcomes. Citation Format: Rachel Mintz, Alexandre Gallerand, Jichang Han, Sarah Ning, Wei Zou, Mary Wohltmann, Bernd Zinselmeyer, Gwendalyn Randolph. Mechanisms underlying the omental support of ovarian cancer peritoneal metastasis [abstract]. In: Proceedings of the AACR Special Conference on Ovarian Cancer; 2023 Oct 5-7; Boston, Massachusetts. Philadelphia (PA): AACR; Cancer Res 2024;84(5 Suppl_2):Abstract nr B091.

Approaches to induce the maturation process of human induced pluripotent stem cell derived-endothelial cells to generate a robust model.

Endothelial cells generated from induced pluripotent stem cells (hiPSC-ECs) show the majority of endothelial cell characteristics and markers, such as cobblestone morphology and the expression of VEGF and VE-cadherin. However, these cells are failing to show a mature endothelial cell phenotype, which is represented by the low expression and production of von Willebrand Factor (VWF) leading to the round morphology of the Weibel Palade Bodies (WPBs). The aim of this study was to improve the maturation process of hiPSC-ECs and to increase the levels of VWF. hiPSC-ECs were differentiated by a standard differentiation protocol from hiPSCs generated from healthy control donors. To induce maturation, the main focus was to increase the expression and/or production of VWF by the adjustment of potential parameters influencing differentiation and maturation. We also compared alternative differentiation protocols. Cells were analyzed for the expression of endothelial cell markers, WPB structure, and the production and secretion of VWF by flow cytometry, confocal microscopy and ELISA. The generated hiPSC-ECs have typical endothelial cell surface expression profiles, with low expression levels of non-endothelial markers as expected. Co-culture with pericytes, varying concentrations and timing of differentiation factors, applying some level of flow, and the addition of HDAC inhibitors did not substantially improve maturation of hiPSC-ECs. Transfection with the transcription factor ETV2 to induce a faster hiPSC-EC differentiation process resulted in a limited increase in VWF production, secretion, and elongation of WPB structure. Alternative differentiation protocols had limited effect. hiPSCs-ECs have the potential to show a more mature endothelial phenotype with elongated WPBs after >30 days in culture. However, this comes with limitations as there are very few cells detected, and cells are deteriorating after being in culture for extended periods of time.

Characterizing the Individuals Who Regain or Maintain Walking Ability after a Hip Fracture: Insights Into Physical Resilience

ObjectivesMaintaining walking ability is key to healthy aging. Hip fractures often lead to declined walking ability. This study investigated characteristics of individuals who regained walking ability after a hip fracture, an expression of physical resilience. DesignRegister-based cohort study. Setting and ParticipantsA total of 55,467 Swedish residents aged ≥60 years with a first hip fracture (71% women, mean age = 82.3 ± 8) included in the Swedish Hip Fracture Register. MethodsInformation about diseases, medications, and socioeconomic (SES) factors came from registers. Individuals were classified by prefracture walking ability (independent or assisted walking) and whether their walking ability 4 months post-fracture was maintained (physical resilience or nonresilience). Cluster analyses were conducted among individuals who maintained their walking ability to assess different physical resilience profiles. ResultsAt baseline, 38,493 individuals walked independently (69%), and 16,982 were assisted walkers. Half of the independent walkers maintained their walking ability 4 months post-fracture. Among them, 3 clusters were identified: a “Low SES, Low Disease” cluster (n = 8580, mean age 81.1 ± 7.5); a “High SES, Low Disease” cluster (n = 7778, mean age 76.7 ± 7.4); and a third “High SES, High Disease” cluster (n = 4320, mean age 77.7 ± 7.4).Sixty percent of the pre-assisted walkers maintained their level of assisted walking ability. Also among them, 3 clusters were identified: a “Low SES-Independent Living” cluster (n = 3077, mean age 85.5 ± 7.1); a second “Care Home” cluster (n = 2912, mean age 87.0 ± 6.5) with a high proportion with dementia diagnosis; and a last “High SES” cluster (n = 4044, mean age 83.0 ± 7.0) with the largest proportion of men. Conclusions and ImplicationsPhysical resilience is not characterized by one typical healthy profile, and it is possible to regain walking ability after a hip fracture despite unfavorable prerequisites in 1 domain. A favorable status in one domain may compensate for an unfavorable status in another, for example, a high disease burden in combination with high SES.

The model of information resources selection based on the generation theory, scientometrics and personality study factor method as an instrument for developing global digital platforms

The authors discuss the methodology of global digital platforms development in the context of Russia’s transfer to the information society. The authors apply the theory of generations, statistical data provided by ROSSTAT and the Ministry of Science and Higher Education, to identify the most promising class of global digital platforms – that is the classes that comprise personality-oriented adaptive educational systems and library information systems providing data to build the curriculum. The essential criteria for transforming information library systems into smart libraries are suggested. Based on the criteria, the literature selection algorithm is developed. It is based on the cluster analysis of R. Cattell’s personality multifactor testing, form C. The selected literature is studied with the subsequent formation of average typical user profiles within lumped program tracks. The search sample is reduced through the modified method of science citation index assessment applied to conference proceedings and academic journals, which is the new approach to monographs and textbooks selection, and typical user psychological and emotional profiles. The authors provide recommendations on implementing their findings in the CIS and RF-friendly countries.

Absorption Troughs of Lyα Emitters in HETDEX

The Hobby–Eberly Telescope Dark Energy Experiment (HETDEX) is designed to detect and measure the redshifts of more than 1 million Lyα emitting galaxies (LAEs) 1.88 < z < 3.52. In addition to its cosmological measurements, these data enable studies of Lyα spectral profiles and the underlying radiative transfer. Using the roughly half a million LAEs in the HETDEX Data Release 3, we stack various subsets to obtain the typical Lyα profile for the z ∼ 2–3 epoch and to understand their physical properties. We find clear absorption wings around Lyα emission, which extend ∼2000 km s−1 both redward and blueward of the central line. Using far-UV spectra of nearby (0.002 < z < 0.182) LAEs in the COS Legacy Archive Spectroscopic Survey treasury and optical/near-IR spectra of 2.8 < z < 6.7 LAEs in the Multi Unit Spectroscopic-Wide survey, we observe absorption profiles in both redshift regimes. Dividing the sample by volume density shows that the troughs increase in higher-density regions. This trend suggests that the depth of the absorption is dependent on the local density of objects near the LAE, a geometry that is similar to damped Lyα systems. Simple simulations of Lyα radiative transfer can produce similar troughs due to absorption of light from background sources by H i gas surrounding the LAEs.

PITX2 as a Sensitive and Specific Marker of Midgut Neuroendocrine Tumors: Results from a Cohort of 1157 Primary Neuroendocrine Neoplasms

As neuroendocrine tumors (NETs) often present as metastatic lesions, immunohistochemical assignment to a site of origin is one of the most important tasks in their pathologic assessment. Because a fraction of NETs eludes the typical expression profiles of their primary localization, additional sensitive and specific markers are required to improve diagnostic certainty.We investigated the expression of the transcription factor Pituitary Homeobox 2 (PITX2) in a large-scale cohort of 909 NET and 248 neuroendocrine carcinomas (NEC) according to the immunoreactive score (IRS) and correlated PITX2 expression groups with general tumor groups and primary localization.PITX2 expression (all expression groups) was highly sensitive (98.1%) for midgut-derived NET, but not perfectly specific, as non–midgut NET (especially pulmonary/duodenal) were quite frequently weak or moderately positive. The specificity rose to 99.5% for a midgut origin of NET if only a strong PITX2 expression was considered, which was found in only 0.5% (one pancreatic/one pulmonary) of non–midgut NET. In metastases of midgut-derived NET, PITX2 was expressed in all cases (87.5% strong, 12.5% moderate), whereas CDX2 was negative or only weakly expressed in 31.3% of the metastases. In NEC, a fraction of cases (14%) showed a weak or moderate PITX2 expression, which was not associated with a specific tumor localization.Our study independently validates PITX2 as a very sensitive and specific immunohistochemical marker of midgut-derived NET in a very large collective of neuroendocrine neoplasms. Therefore, our data argue toward implementation into diagnostic panels applied for NET as a firstline midgut marker.

TSR-Based optimization using auxiliary motor in Tidal Energy Conversion System: An experimental study

The fluctuating nature of renewable energy is one of the factors that reduces its ability to compete with traditional forms of power generation. In the context of ocean current energy, tidal oscillations are the primary reason for the average power generation being significantly lower than the designed value. Increasing energy production through optimization is necessary for ocean current energy to compete successfully in the market. This experimental study is intended to increase electrical power production through electromechanical engineering with tip speed ratio (TSR) control using auxiliary motors, and adapts the Motor Generator Pair (MGP) approach, where the generator and motor are connected in series. A physical model of a Tidal Energy Conversion System (TECS) was developed. The system consists of a turbine simulator in the form of an AC motor with separate torque and speed controls, torque sensors, a hydraulic transmission system, and auxiliary motors. With this system, a typical tidal turbine profile was built and simulated TSR-based optimization control. The results of this optimization control show that power production can be increased through optimal tip-speed ratio control.

1H-NMR Approach for the Discrimination of PDO Grana Padano Cheese from Non-PDO Cheeses

Protected Designation of Origin cheeses are products with high-quality standards that can claim higher prices on the market. For this reason, non-PDO cheeses with lower quality can be mislabeled as PDO or mixed with it for economic gain especially when the product is in a shredded form. Luckily, the production of PDO cheese is subjected to strict procedural specification rules that result in a product with a defined profile of its metabolites, which can be used for authentication purposes. In this study, an NMR metabolomic approach combined with multivariate analysis was implemented to build a classification model able to discriminate PDO Grana Padano cheese from a large dataset of competitors. The great advantage of the proposed approach is a simple sample preparation, obtaining a holistic overview of the analyzed samples. The untargeted approach highlighted a “typical profile” of Grana Padano samples, which could be used for protection purposes. In parallel, the targeted results allowed us to identify potential chemicals, such as lactate, some amino acids and lipids. These initial results could open the road to a potential new additional tool to check the authenticity of PDO cheeses in the future.

What Does Atypical Chronic Lymphocytic Leukemia Really Mean? A Retrospective Morphological and Immunophenotypic Study.

Atypical chronic lymphocytic leukemia (CLL) is still defined according to morphological criteria. However, deviance from the typical surface immunological profile suggests an atypical immunological-based CLL. A large cohort of patients with CLL was retrospectively evaluated aiming at assessing morphological (FAB criteria), immunophenotypical (two or more discordances from the typical profile), and clinical-biological features of atypical CLL. Compared to typical cases, morphologically atypical CLL showed a greater percentage of unmutated IgVH and CD38 positivity, and a higher expression of CD20. Immunophenotypically atypical CLL was characterized by more advanced clinical stages, higher expression of CD20, higher rate of FMC7, CD79b and CD49d positivity, and by an intermediate-high expression of membrane surface immunoglobulin, compared to typical cases. When patients were categorized based on immunophenotypic and morphologic concordance or discordance, no difference emerged. Finally, morphological features better discriminated patients' prognosis in terms of time-to-first treatment, while concordant atypical cases showed overall a worse prognosis. Discordant cases by immunophenotype and/or morphology did not identify specific prognostic groups. Whether-in the era of molecular markers used as prognostic indicators-it does make sense to focus on morphology and immunophenotype features in CLL is still matter of debate needing further research.

Is it really keratoconus ‐ topographic mimicry

Aims/Purpose: The aim of this case series is to present eyes with topographic keratoconus configuration, but in which objectifiable no keratoconus exists and thus a so‐called pseudo‐keratoconus is present.Methods: Inclusion criteria were a topographic classification of the cornea as keratoconus by Scheimpflug imaging using the topographic keratoconus classification TKC and a typical elevation data profile with irregular astigmatism and slope (= Ansteilung) of the cornea. Clinical image by slit lamp examination, best‐corrected visual acuity and Scheimpflug tomography of the anterior segment of the eye (Pentacam®, OCULUS, Wetzlar, Germany) were analysed.Results: In our case series, 33 eyes of 23 patients were included. The mean age was 51 (±16) years and the ratio of females to males was balanced. The mean Kmax was 51.1 (±7.1) dioptres. The mean corneal thickness at the thinnest point was 517 (±80) μm. The best‐corrected decimal visual acuity was 0.47 (±0.27). The study eyes were divided into 3 groups according to the underlying cause of the topographic changes. One group included corneal diseases with inferior slope in topography (corneal scar n = 9, Map‐Dot‐Fingerprint dystrophy n = 6, Fuchs endothelial dystrophy n = 5, vortex keratopathy n = 2). The second group had a superior corneal slope that could be interpreted as a superior keratoconus configuration (Map‐Dot‐Fingerprint dystrophy n = 5, irregular astigmatism without other corneal pathology n = 2, Salzmann corneal degeneration n = 1, corneal scar n = 1). The third group included eyes with postoperative newly occurring keratoconus configuration after phototherapeutic keratectomy (n = 2).Conclusions: This case series shows that different corneal pathologies can imitate a topographic keratoconus configuration. The identification – not least at the slit lamp – and differentiation of these pathologies from keratoconus are important in daily practice with regard to counselling of the patients and further treatment.

Selecting Monoclonal Cell Lineages from Somatic Reprogramming Using Robotic-Based Spatial-Restricting Structured Flow.

Somatic cell reprogramming generates induced pluripotent stem cells (iPSCs), which serve as a crucial source of seed cells for personalized disease modeling and treatment in regenerative medicine. However, the process of reprogramming often causes substantial lineage manipulations, thereby increasing cellular heterogeneity. As a consequence, the process of harvesting monoclonal iPSCs is labor-intensive and leads to decreased reproducibility. Here, we report the first in-house developed robotic platform that uses a pin-tip-based micro-structure to manipulate radial shear flow for automated monoclonal iPSC colony selection (~1 s) in a non-invasive and label-free manner, which includes tasks for somatic cell reprogramming culturing, medium changes; time-lapse-based high-content imaging; and iPSCs monoclonal colony detection, selection, and expansion. Throughput-wise, this automated robotic system can perform approximately 24 somatic cell reprogramming tasks within 50 days in parallel via a scheduling program. Moreover, thanks to a dual flow-based iPSC selection process, the purity of iPSCs was enhanced, while simultaneously eliminating the need for single-cell subcloning. These iPSCs generated via the dual processing robotic approach demonstrated a purity 3.7 times greater than that of the conventional manual methods. In addition, the automatically produced human iPSCs exhibited typical pluripotent transcriptional profiles, differentiation potential, and karyotypes. In conclusion, this robotic method could offer a promising solution for the automated isolation or purification of lineage-specific cells derived from iPSCs, thereby accelerating the development of personalized medicines.

Solar wind entry into Mercury’s magnetosphere: Simulation results for the second swingby of BepiColombo

Context. We use a global 3D hybrid plasma model to investigate the interaction between Mercury’s magnetosphere and the solar wind for the second BepiColombo swingby, evaluate magnetospheric regions, and study the typical energy profile of protons. Aims. The objective of this study is to gain a better understanding of solar wind entry and analyze simulated plasma data along a trajectory using BepiColombo swingby 2 conditions, with the goal of enhancing our comprehension of measurement data and potentially providing forecasts for future swingbys. Methods. To model Mercury’s plasma environment, we used the hybrid code AIKEF and developed a method to extract the particle (ion) data in order to compute the proton energy spectrum along the trajectory of BepiColombo during its second Mercury swingby on June 23, 2022. We evaluate magnetopause and bow shock stand-off distances under average upstream solar wind conditions with the Interplanetary Magnetic Field (IMF) condition derived from the BepiColombo magnetic field measurements during the second Mercury swingby. Results. We found that the magnetosheath on the quasi-perpendicular (dusk) side of the bow shock is thicker than that on the quasi-parallel (dawn) side, where a foreshock is formed. Multiple plasma populations can be extracted from our modeled energy spectra that assist in identifying magnetospheric regions. We observed protons of solar wind origin entering Mercury’s magnetosphere. Their energies range from a few electron volts in the magnetosphere up to 10 keV in the magnetosheath.

A solution approach for decision-making on vessel fleet sustainability focusing on alternative fuels in a life-cycle basis

International Maritime Organization has launched a set of different regulatory frameworks focusing on maritime pollution prevention mechanisms and practicing different solution approaches. A multi-dimensional life-cycle analysis on vessels and maritime fuels (fossil & alternative low carbon fuels) can make an important contribution to the IMO's long-term carbon neutrality target for 2050. A typical vessel's operational profile contributes on approx. 80% of greenhouse gas emissions and 50% of total costs respectively during its lifetime. Therefore, the investigation of ships operational benchmarks following a lifecycle analysis approach could create suitable decision-making support tools for different optimization problems regarding alternative green fuels. This paper proposes a solution approach to evaluate current vessel fleet operational benchmarks for different ship categories and provide a decision-making support mechanism to tackle important operational problems during ship's lifetime. The proposed approach contains an evaluation mechanism to determine the suitable economic and environmental key performance indicators necessary to properly evaluate the multi-dimensional sustainability of the examined case studies with respect to the IMO (MARPOL) environmental regulations.

RESEARCH ON THE PRE-TRAVEL BEHAVIOR OF THE BULGARIAN TRAVELERS

This research paper explores and discusses the behavior of the Bulgarian non-organized travelers concerning their travel destination and accommodation choices before the pandemic period. Another study goal of the paper is to identify the typical segment profiles of the Bulgarian travelers. The main research method was a content analysis of the information from the open-ended questions to the members of a closed Bulgarian group “Travelling in the world” in Facebook. The survey sample consists of 164 travelors who responded on a voluntary basis to questions related to their pre-travel behavior. Additional primary information was derived from the comments of the group members about their travel behavior. Тhe study identified 3 main segments among the Bulgarian travelers with different pre-travel behavior called by the authors “Comfort Travelers”, “Adventurers” and “Travelers around”. The final part of the paper includes conclusions from the study about the Bulgarian travelers’ behavior on behalf of destination and accommodation choice before the pandemia and some practical suggestions to the hoteliers about using more in-depth surveys with travelers and content marketing for attracting and retaining guests.

Typische Profile digitaler Kompetenzen: Eine clusteranalytische Untersuchung digitaler Kompetenzen im Arbeitskontext

ZusammenfassungTypologien reduzieren die Vielfalt menschlicher Merkmale auf eine geringe Zahl an Merkmalsausprägungen, die die Wirklichkeit vereinfachend abbilden und so die Ableitung organisationaler Interventionen ermöglichen. Für den Umgang von Menschen mit der Digitalisierung wurden bereits mehrere Typologien mit vier bis sieben Typen bzw. Clustern aufgestellt, wobei dies z. B. auf Basis der Intensität der Mediennutzung, der Begeisterung für digitale Umbrüche oder der Fähigkeiten der Beschäftigten geschah. In der vorliegenden Arbeit werden neun digitale Kompetenzen von n = 645 Beschäftigten im Rahmen einer Fragebogenstudie (Oktober 2021–April 2022) erfasst. Mithilfe einer Clusteranalyse werden vier typische Profile digitaler Kompetenzen identifiziert, die als (1) begeistertes, (2) balanciertes, (3) naives und (4) ablehnendes Cluster bezeichnet werden. Die vier Cluster zeigen Zusammenhänge zu den wahrgenommenen organisationalen Lernmöglichkeiten, zum Techno-Engagement und zum Persönlichkeitsmerkmal der Offenheit. Sie spiegeln bisherige Forschung zur Typologisierung von Beschäftigten im Umgang mit der Digitalisierung präzise wider. Somit stellen sie ein generalisierbares, stabiles und bedeutsames Muster dar, das für organisationale Interventionen zur Steigerung der digitalen Kompetenzen und der Bereitschaft für digitale Umbrüche genutzt werden kann.

Available Energy Analysis of Electric Unmanned Aerial Vehicle Hybrid Energy System

The issue of energy and power systems is the main challenge faced by electric unmanned aerial vehicles for long endurance. Under the power demand conditions of typical mission profiles of electric drones, this paper establishes an energy balance equation between the hybrid energy system power supply of electric drones and the power demand of drones. Considering the impact of operating temperature of each battery on power generation performance, a heat transfer model for solar cells, fuel cells, and lithium batteries was established. The heat transfer process of hybrid energy systems was analyzed. Based on the heat transfer model of hybrid energy systems, the heat transfer changes of solar cells, fuel cells, and lithium batteries were analyzed, and the heat transfer changes of hybrid energy systems were studied. The heat loss changes of each battery subsystem with operating temperature were given. The results show that the hybrid energy system has the best power generation performance at the reference temperature, and changes in operating temperature will cause thermal losses and reduce power generation.