Rockburst has become a main factor threatening the safe construction of deep-buried tunnels. To monitor microseismic (MS) event and comprehend evolution process of rockbursts, a MS monitoring system was established in a deep-buried tunnel excavated by drilling and blasting. The mechanisms of three typical rockburst cases were investigated through the analysis of the MS sequence and development process. The results showed that the MS sequence of first rockburst case was a main-aftershock type, corresponding to a strain rockburst that occurred in intact surrounding rocks. Most of the MS events were failure in tensile, with few MS events presenting mixed failure mode. For the second rockburst case, the MS sequence was a fore-main-aftershock type, corresponding to a strain-structural slip rockburst that occurred in surrounding rocks with a considerable number of small-scale structural planes. The MS events in tensile failure were predominated, and a few MS events were failure in mixed and shear. The MS sequence of the third rockburst case was a new type observed in the present study, i.e. main shock type, corresponding to a strain-structural slip rockburst. It occurred in high strength surrounding rocks with rigid structural planes, which easily induced collapse in the rockburst process. The MS events in tensile failure were predominated. The precursor phenomena of former two cases of the rockburst can be identified through the analysis of MS sequence and source parameters. While before the third rockburst case occurred, no or a few MS events were obtained. It is difficult to predict this type of rockburst based on source parameters. For this case, destress blasting method was adopted. The number of high-energy MS events decreased abruptly, and no intense rockbursts occurred. This method can effectively release the concentrated stress of the surrounding rocks and reduce the risk of rockbursts. The results of this study could provide guidance and reference for the early warning and prevention of rockbursts in deep-buried tunnels.