To study the antioxidant effect of the aqueous extracts of Typhonium giganteum Engl. tuber (AEoTGE) and ethanol extracts of Typhonium giganteum Engl. tuber (EEoTGE) in vitro and in vivo, which may be the possible mechanism of its bioactivity efficacy. The antioxidant activity in vitro was evaluated by 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) radical scavenging rate with Vitamin C as a positive control. The antioxidant activity in vivo was carried out to investigate the effects of AEoTGE and EEoTGE on D-galactose caused aging model mice by determining liver tissues’ superoxide dismutase (SOD) activities. The results indicated that AEoTGE and EEoTGE have good antioxidant activity in vivo and vitro. The antioxidant effect of AEoTGE is better than that of EEoTGE.