Reactive oxygen species(ROS) involving in inhibiting proliferation of human bone marrow neuroblastoma cells (SH-SY5Y) by giant typhonium rhizome water extract

Abstract

Objective To investigate whether the reactive oxygen species involved in inhibiting proliferation of human bone marrow ceils of neuroblastoma cells （SH-SY5Y） process by giant typhonium rhizome water extract, and to investigate the initial mechanism of anti-tumor effect of giant typhonium rhizome in vitro. Methods After giant typhonium rhizome water extract processing of human bone marrow neuroblastoma cells （SH-SY5Y）at 24 h and 48 h, the cells were collected, and western blotting reaction methods such as flow cytometry was used to study the levels of ROS and SH-SY5Y apoptosis. Results The levels of ROS elevated fxom before dosing （4.9± 1.6）% to （39.7±11.8）% in the process of giant typhonium rhizome water extract induced apoptosis in SH-SY5Y; after ROS inhibition was added, SH-SY5Y apoptosis level induced by giant typhonium rhizome water extract had decreased. Conclusion ROS involved in the process of giant typhonium rhizome water extract inhibiting cell proliferation in SH-SY5Y. Key words: Giant typhonium rhizome; Reactive oxygen species; Human bone marrow neuroblastoma cells： Flow cytometry