Effect of miR-92 on the proliferation and apoptosis of human neuroblastoma cells

Abstract

Objective To investigate the effects of miR-92 on the proliferation and apoptosis in human neuroblastoma (NB) cells,and to predict its putative targets.Methods Antisense oligonucleotides of miR-92 were synthesized and transfected into SK-N-SH and SK-N-BE2 cells,then real time PCR was performed for the detection of miR-92 expression.Cell proliferation and apoptosis were determined using MTT assay and flow cytometry.The putative targets for miR-92 were predicted using bioinformatics.Results Cell viability and proliferation of anti-miR-92 transfected cells were reduced by 20％ and 50％-60％,respectively,while apoptosis increased by 2-3 fold (P＜0.05).Using bioinformatics,BCL2-like 11 (apoptosis facilitator) (BCL2L11) and PTEN were selected as the candidate targets for miR-92.Conclusions Our data indicate that the inhibition of miR-92 can suppress NB cell proliferation and increase cell apoptosis,suggesting miR-92 may play important roles in NB growth.In addition,miR-92 may serve as a novel biomarker for therapeutic strategy of NB patients. Key words: Neuroblastoma; MicroRNAs; Cell proliferation; Apoptosis