Dental plaque is the principal etiological agent for the development and progression of gingival andperiodontal diseases. Three different mouthwash types were compared in this study for the periodontalhealing process based on the results. For all bacterial strains, the culture type collection is used. The serialdilution process is used for preparing every mouthwash and adding it to the tubes with a specific dilution. Incertain microorganisms of the respective mouthwash, the last tube dilution that does not show any turbiditywill be considered MIC. The three types of mouthwashes were compared in terms of the ability to inhibitmicrobial growth. Twenty-four hours after the agar was solidified and the colony forms (CFU) units counted,the plates were incubated. This study showed that bacterial growth inhibition might occur in the three typesof mouthwash. Boht, Behsa and Kin-gingival mouthwashes had a significant difference in their antimicrobialeffect. This study showed that they were unable to perform s after 24 hours of in vitro incubation while thebacterial count was lowered after two weeks of in vivo use in the boht washings. Continuous exposure tobacteria may be necessary to wash the mouth, especially for Behsa and Boht. It is difficult to remove allbacteria from the mechanical plaque controls; antibacterial mouthwash can cause additive. In the currentstudy, three types of mouthwash have compared antimicrobial effects. The results showed only that bacterialcounts are affected by mouthwash. Based on this study’s results, Boht mouthwash is more effective thanKin Gingival and Behsa mouthwash for oral microorganisms, but further clinical studies are necessary toconfirm our findings.