Types Of Liver Lesions Research Articles

Comparative evaluation of volumetry estimation from plain and contrast enhanced computed tomography liver images.

Surgery planning for liver tumour is carried out using contrast enhanced computed tomography (CECT) images to determine the optimal resection strategy and to assess the volume of liver and tumour. Current surgery planning tools interpret even the functioning liver cells present within the tumour boundary as tumour. Plain CT images provide inadequate information for treatment planning. This work attempts to address two shortcomings of existing surgery planning tools: (i) to delineate functioning liver cells from the non-functioning tumourous tissues within the tumour boundary and (ii) to provide 3D visualization and actual tumour volume from the plain CT images. All slices of plain CT images containing liver are enhanced by means of fuzzy histogram equalization in Non-Subsampled Contourlet Transform (NSCT) domain prior to 3D reconstruction to clearly delineate liver, non-functioning tumourous tissues and functioning liver cells within the tumour boundary. The 3D analysis from plain and CECT images was carried out on five types of liver lesions viz. HCC, metastasis, hemangioma, cyst, and abscess along with normal liver. The study resulted in clear delineation of functional liver tissues from non-functioning tumourous tissues within the tumour boundary from CECT as well as plain CT images. The volume of liver calculated using the proposed approach is found comparable with that obtained using Myrian-XP, a currently followed surgery planning tool in clinical practice. The obtained results from plain CT images will undoubtedly provide valuable diagnostic assistance and surgery planning even for the subset of patients for whom CECT acquisition is not advisable.

Hepatopathies of monogastric animals under the conditions of the Northern Trans-Urals

As is known, the liver in the body of mammalian animals converts nutrients into other chemical formations, which are later used by the body itself or are excreted. Also, this organ performs a detoxification function. The liver contains the necessary supply of blood, vitamins and carbohydrates for the animal, as well as the synthesis of some blood proteins and other vital organic substances. It is known that up to 1,000 different biochemical processes take place in this largest gland of the body, however, due to the enormous load on the hepatocyte, it often undergoes destruction. The level and intensity of metabolic processes in the body of animals depends on the morphofunctional state of this organ. The aim of the research was to study the spread and manifestation of hepatopathy in monogastric animals in the conditions of the Northern Trans-Urals. The liver of the studied animals (rabbits and dogs) was selected for morphohistological studies. Based on the conducted studies and analysis of the results obtained, it was found that various types of hepatopathy in rabbits with unbalanced feeding and feeding with substandard feed were observed in 45% of cases, and in dogs - in 41.7% of cases. For the digestive glands, both substandard commercial feed and feed prepared by the owners of animals themselves have a negative impact. At the same time, the degree of liver dysfunction did not depend on whether the animals were fed industrial feed or food "from the table". In the course of our research, the following data were obtained: during morphological examination of the liver of rabbits, pathologies were established in 45% of cases, of which hydropic dystrophy -10%, hepatosis -20%, cirrhosis -15%. Liver dysfunction, as an independent disease, was registered in dogs in 14.1% of cases. In experimental dogs, we treated the following types of liver lesions: 42% - liver steatosis, 24.5% - liver inflammation, 12% - liver cirrhosis, 19.2% - cholecystitis, 2.3% - liver neoplasms.

Optimization of FireNet for Liver Lesion Classification

In recent years, deep learning techniques, and in particular convolutional neural networks (CNNs) methods have demonstrated a superior performance in image classification and visual object recognition. In this work, we propose a classification of four types of liver lesions, namely, hepatocellular carcinoma, metastases, hemangiomas, and healthy tissues using convolutional neural networks with a succinct model called FireNet. We improved speed for quick classification and decreased the model size and the number of parameters by using fire modules from SqueezeNet. We have used bypass connection by adding it around Fire modules for learning a residual function between input and output, and to solve the vanishing gradient problem. We have proposed a new Particle Swarm Optimization (NPSO) to optimize the network parameters in order to further boost the performance of the proposed FireNet. The experimental results show that the parameters of FireNet are 9.5 times smaller than GoogLeNet, 51.6 times smaller than AlexNet, and 75.8 smaller than ResNet. The size of FireNet is reduced 16.6 times smaller than GoogLeNet, 75 times smaller than AlexNet and 76.6 times smaller than ResNet. The final accuracy of our proposed FireNet model was 89.2%.

A study on Ultra sound guided FNAC in Liver lesions in a tertiary government hospital

Introduction: Lesions of liver are generally evaluated by Ultrasound guided FNAC. It is useful for differentiate from Non-neoplastic to neoplastic lesions of liver. AIM: To analyse the various types of liver lesions according to age and sex of the patients. 2. To study the Cytomorphological features of liver aspirate. Methods: Present study done over a period of two years, ie 2016-2018. A total number of 86 cases were studied in the Department of Pathology, Govt. Medical College, Kadapa, AP. All these cases were categorised based on Cytomorphological features, obtained by Fine needle aspiration. Materials required for FNAC: 10 ml sterile disposable syringe with 25-22G needles. Suitable fixative is Isopropyl Alcohol. Alcohol fixed slides taken up for H&E staining. Results: FNAC of liver were performed on 86 cases. Out of 86 cases primary malignant lesions are 32 (37.20%), Metastatic lesions are 53 (61.62%), One case of Hepatoblastoma (1.16%).

Classification of Liver Diseases Based on Ultrasound Image Texture Features

This paper discusses using computer-aided diagnosis (CAD) to distinguish between hepatocellular carcinoma (HCC), i.e., the most common type of primary liver malignancy and a leading cause of death in people with cirrhosis worldwide, and liver abscess based on ultrasound image texture features and a support vector machine (SVM) classifier. Among 79 cases of liver diseases including 44 cases of liver cancer and 35 cases of liver abscess, this research extracts 96 features including 52 features of the gray-level co-occurrence matrix (GLCM) and 44 features of the gray-level run-length matrix (GLRLM) from the regions of interest (ROIs) in ultrasound images. Three feature selection models—(i) sequential forward selection (SFS), (ii) sequential backward selection (SBS), and (iii) F-score—are adopted to distinguish the two liver diseases. Finally, the developed system can classify liver cancer and liver abscess by SVM with an accuracy of 88.875%. The proposed methods for CAD can provide diagnostic assistance while distinguishing these two types of liver lesions.

Evaluation of ADCratio on liver MRI diffusion to discriminate benign versus malignant solid liver lesions

Purpose: The aim of this project is to investigate the usefulness of the absolute liver lesion ADC value and ratio of Apparent diffusion coefficient (ADC) values of a liver lesion and liver parenchyma to discriminate between a benign and malignant lesion.Methods: Liver MRI scans performed between January 2009 and June 2015 were retrospectively analysed. Scans were performed on either a 1.5 T or 3 T MRI unit. The type of liver lesion (benign or malignant) was determined by its radiological appearance, histology result and clinical management. Lesions with undetermined diagnosis or MRI studies degraded by artifacts were excluded. Liver cysts were also excluded from the analysis. ADC value of a lesion and liver parenchyma was measured and ADCratio was calculated. The values were analysed using independent samples t-testResults:Data set contained 39 benign lesions and 36 malignant lesions. Mean ADC value for benign lesions was 1678, and the mean value for malignant lesions was 1097 with a statistically significant difference of p < 0.001. All lesions with ADC value below 955 were malignant, while all lesions with ADC value above 1880 were benign. ADC value of 1260 was identified as the best available cut-off value for differentiating benign and malignant lesions, achieving sensitivity of 92%, specificity of 80% and an overall accuracy of 89%. The mean lesion to liver ADCratio for benign lesions was 1.3467 and for malignant lesions was 0.9038 with a statistically significant difference of p < 0.001. All lesions with ADCratio measuring <0.9 were malignant while lesions with ADCratio>1.5 were benign. ADCratio of 1.1 was identified statistically as the best available cut-off value for differentiating benign from malignant lesions, with sensitivity of 82%, specificity of 86% and an overall accuracy of 92%.Conclusion:Our dataset indicates that lesion to background liver ADCratio is superior in discriminating between benign and malignant focal lesions compared to absolute ADC values of the hepatic lesions.

Sa1008 Evaluation of Stiffness of the Spleen (Fibrospleen) in a Real Life Cohort of Patients With All Stages of Liver Diseases

Background and aim. Contrast enhanced ultrasound (CEUS) has improved the diagnostic work up of focal liver lesions and, according to international guidelines, is often used as the first method of choice in differentiating benign from malign lesions. However, interpretation of the contrast-enhancement patterns is a subjective modality with a certain degree of inter-observer variability. In the last years, quantitative approaches able to measure the amount and the time course of contrast uptake have shown great promise in revealing tumour response to anti-angiogenic drugs in patients with different types of malignant lesions. We evaluate the feasibility of dynamic CEUS (D-CEUS) with perfusion software as a diagnostic tool in characterizing different types of liver lesions. Patients and methods. CEUS with second generation contrast agent (Sonovue, Bracco) was performed in 22 benign and 15 malignant hepatic lesions in 32 patients 15 female and 17 male; aged 51±12 years). All cases were confirmed by MRI. Lesion vasculature was assessed in a specific harmonic mode associated with a perfusion and quantification software (Q-Lab, Philips). Five D-CEUS functional parameters (peak intensity, PI; time to PI, TP; area under the curve, AUC; slope of wash in, Pw; mean transit time, MTT) were extracted from time intensity curves and were compared among groups and applied to characterize different types of lesions. Statistical analysis was performed by a non-parametric test and a p value < 0.01 was considered statistically significant. Results. We report the preliminary results obtained in 14 hemangiomas, 8 focal nodular hyperplasias (FNH) and 20 hepatocellular carcinomas (HCC). Comparison between benign and malignant lesions revealed no statistically significant differences in all the perfusion parameters except for MTT that was longer in the first group (p = 0.005). For individual analysis, PI, AUC, and Pwi, were significantly higher in FNH than both in hemangiomas and HCC, while TTP was significantly shorter in FNH than in HCC. When we compared hemagiomas and HCC, PI, Pwi and TTP were significantly lower in the former than in the latter. Conclusion. Quantitative analysis provide a more reliable characterization of different types of liver lesions and could be an excellent tool to improve the diagnostic accuracy of CEUS. However further studies with a greater number of patients are required to confirm these results.

Hepatoprotective Effect of Sesame Oil against Lead Induced Liver Damage in Albino Mice: Histological and Biochemical Studies

The liver performs many vital functions to eliminate toxins and harmful substances from the body. Hepatotoxic agents can react with the basic cellular components and consequently induce almost all types of liver lesions. The aim of this study was to investigate the possible hepatoprotective role of sesame oil against lead acetate induced hepatotoxicity in albino mice from the histological and biochemical aspects. In this study, thirty two adult male albino mice were used for this study and divided into four groups. The first group was control group, the 2nd was the sesame oil group orally received sesame oil (5 ml/kg body wt.), the 3rd group was the experimental and received lead acetate (500 mg /kg diet), the 4th one co-administered lead acetate (500 mg/kg diet) with sesame oil (5 ml/kg body wt.) daily for 30 days. The livers were dissected out, weighted and specimens were taken and processed for light microscopic examinations. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities, serum total proteins and albumin. Results indicate that, in lead treated animals, there were severe structural damage in the liver. The hepatocytes appeared irregularly arranged with disorganization of hepatic architecture. The hepatocytes appeared large with light and foamy cytoplasm filled with numerous vacuole-like spaces. The nuclei appeared with pyknotic nuclei. The central vein appeared dilated and congested with massive hemorrhage extending to the nearby cells. Also, there were focal degenerative and necrotic changes along with inflammatory cell infiltration. Decrease in body weight and increase in liver weight were observed. Biochemically, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, and γ- glutamyltransferase activities were increased and serum total proteins and albumin were decreased. Co-administration of sesame oil significantly improved the structural changes in the liver and also the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase and γ- glutamyltransferase activities were significantly declined and serum total proteins and albumin were elevated. Conclusion: It can be concluded that, the lead had adverse effects on the liver. Sesame oil showed effective hepatoprotective action against lead acetate induced hepatotoxicity in albino mice. So, the populations of high risk to lead should be advised to take sesame oil.

Effect of Cadmium on the Liver and Amelioration by Aqueous Extracts of Fenugreek Seeds, Rosemary, and Cinnamon in Guinea pigs: Histological and Biochemical Study

Hepatotoxic agents can react with the basic cellular components and consequently induce almost all types of liver lesions. Objective: The present work aimed to evaluate effectiveness of fenugreek seeds, rosemary and cinnamon against cadmium induced hepatotoxicity in guinea pigs from the histological and biochemical aspects. Materials and methods: 48 guinea pigs were used for this study and divided into 8 groups. The first 4 groups were control groups, the 5th group was the experimental and administered oral cadmium chloride at a dose of 5 mg/kg. body weight./day for 28 days, the 6th, 7th, and 8th groups co-administered cadmium with aqueous extracts of fenugreek seeds, rosemary and cinnamon at a dose of 150 mg, 220 mg, and 200 mg/ kg body weight /day, respectively. The livers were dissected out, weighted and specimens were taken and processed for light microscopic examinations. Blood samples were obtained for assessment of serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase, γ- glutamyltransferase activities, and serum total and direct bilirubin. Results: In cadmium treated animals, there were severe structural damage in the liver. Most of hepatocytes appeared fused together forming eosinophilic syncytial masses. The hepatocytes appeared irregularly arranged with disorganization of hepatic architecture. The hepatocytes appeared large with light and foamy cytoplasm filled with numerous vacuole-like spaces. The nuclei appeared with pyknotic nuclei. The central vein appeared dilated and congested with massive hemorrhage extending to the nearby cells. Mild periductal fibrosis around bile duct in the portal area were observed. Also, there were focal degenerative and necrotic changes along with inflammatory cell infiltration. Decrease in body weight and increase in liver weight were observed. Biochemically, the serum alanine aminotransferase, aspartate aminotransferase, alkaline phosphatase), and γ- glutamyltransferase activities, serum total and direct bilirubin were elevated. Co-adminstration of fenugreek, rosemary and cinnamon significantly improved the structural changes in the liver and also all the above mentioned biochemical parameters were significantly declined. Conclusion: It can be concluded that, the cadmium had adverse effects on the liver. Aqueous extracts of different natural materials as Fenugreek, rosemary and cinnamon were able to attenuate these effects. So, the populations of high risk to cadmium should be advised to take one of these materials.

Current Status of Stem Cell Therapy for Liver Diseases

Liver failure is one of the main causes of death worldwide and is a growing health problem. Since the discovery of stem cell populations capable of differentiating into specialized cell types, including hepatocytes, the possibility of their utilization in the regeneration of the damaged liver has been a focus of intense investigation. A variety of cell types were tested both in vitro and in vivo, but the definition of a more suitable cell preparation for therapeutic use in each type of liver lesions is yet to be determined. Here we review the protocols described for differentiation of stem cells into hepatocytes, the results of cell therapy in animal models of liver diseases, as well as the available data of the clinical trials in patients with advanced chronic liver disease.

Application of an artificial neural network to the computer-aided differentiation of focal liver disease in MR imaging

The differentiation of focal liver lesions in magnetic resonance (MR) imaging is primarily based on the intensity and homogeneity of lesions with different imaging sequences. However, these imaging findings are falsely interpreted in some patients because of the complexities involved. Our aim is to establish a computer-aided diagnosis system named LiverANN for classifying the pathologies of focal liver lesions into five categories using the artificial neural network (ANN) technique. On each MR image, a region of interest (ROI) in the focal liver lesion was delineated by a radiologist. The intensity and homogeneity within the ROI were calculated automatically, producing numerical data that were analyzed by feeding them into the LiverANN as inputs. Outputs were the following five pathologic categories of hepatic disease: hepatic cyst, hepatocellular carcinoma, dysplasia in cirrhosis, cavernous hemangioma, and metastasis. Of the 320 MR images obtained from 80 patients (four images per patient) with liver lesions, our LiverANN classified 50 cases of a training set into five types of liver lesions with a training accuracy of 100% and 30 test cases with a testing accuracy of 93%. The experiment demonstrated that our LiverANN, which functions as a computer-aided differentiation tool, can provide radiologists with a second opinion during the radiologic diagnostic procedure.

Incidentalomes

A liver incidentaloma corresponds to a liver lesion detected during work-up of for unrelated pathology. The frequency of incidentalomas is directly related to the incidence of liver tumors in the general population. After detection of an incidental liver lesion, the goal is to assess if diagnosis can be made based on imaging features alone or if biopsy is required. As such, radiologists must be familiar with the imaging features of different types of liver lesions, irrespective of the imaging modality. US is useful to determine the number of lesions (single versus multiple) and the cystic or solid nature of each lesion. Cystic lesions are usually benign. Incidental solid liver lesions are typically benign, but detection of a malignant lesion, hepatocellular carcinoma and metastases, is possible. The most frequent benign incidental solid liver lesions are hemangioma, focal nodular hyperplasia and liver cell adenoma. Accurate imaging diagnosis may not always be achievable, and biopsy, percutaneous or surgical, may be required.

Fat-Containing Lesions of the Liver: Cross-Sectional Imaging Findings with Emphasis on MRI

The purpose of this pictorial essay is to identify different types of liver lesions that contain fat. Cross-sectional imaging findings of fat- or lipid-containing lesions can help in characterizing focal liver lesions. We searched our archive retrospectively and reviewed the literature for fat-containing liver lesions and identified 16 different types. These lesions can contain macroscopic fat (i.e., angiomyolipoma, lipoma, liposarcoma, hydatid cyst, lipopeliosis, adrenal rest tumor, pseudolipoma, hepatic teratoma, pericaval fat, extramedullary hematopoiesis, and metastases) or intracellular lipid (i.e., focal steatosis, adenoma, focal nodular hyperplasia, regenerative nodules, and hepatocellular carcinoma). CT, MRI, and sonographic findings of these lesions can help in characterization by allowing specific diagnosis or narrowing the differential diagnosis of liver lesions.

Hydropic vacuolation in the liver of three species of fish from the U.S. West Coast: lesion description and risk assessment associated with contaminant exposure.

Hydropic vacuolation (HydVac) of biliary epithelial cells and hepatocytes is described for 3 species of U.S. West Coast bottom fishes. Risk assessment analyses are also conducted to determine if the prevalence of this lesion increases in association with contaminant exposure and site of capture. The morphology of HydVac in starry flounder Platichthys stellatus, white croaker Genyonemus lineatus and rock sole Lepidopsetta bilineata was similar to that described in winter flounder Pleuronectes americanus from the U.S. Atlantic Coast, especially in that HydVac most commonly affected biliary epithelial cells. Hydropic vacuolation was the most prevalent liver lesion in starry flounder and white croaker captured from contaminated environments. Risk assessment analyses confirmed that the relative risk for HydVac increased with the presence of aromatic and chlorinated hydrocarbons in sediment, fish bile, and fish liver for these species. Hydropic vacuolation also frequently occurred in rock sole, but the lesion showed no clear association with contaminant exposure in this species. The types of liver lesions that were useful biomarkers of contaminant effects in fish depended on the species and this factor must be taken into account when evaluating histopathological biomarkers of response to contaminant exposure.

Ludwig symposium on biliary disorders--part I. Pathogenesis of ductal plate abnormalities.

Intrahepatic bile ducts (IHBDs) develop from bipotential liver progenitor cells in contact with the mesenchyme of the portal vein and thus form the "ductal plates." The ductal plates are remodeled into mature tubular ducts. Lack of remodeling results in the persistence of periportal epithelial sleeves or "ductal plate malformation" (DPM). A proposal is that virtually all congenital diseases of IHBDs represent examples of DPM. Some early, severe types of extrahepatic bile duct atresia are characterized by DPM, a suggestion of a prenatal beginning of the disease. Several congenital diseases are characterized by dilatation of segments of IHBDs and variable degrees of fibrosis. Such "fibrocystic diseases" represent DPM at different levels of the biliary tree. Autosomal recessive polycystic kidney disease represents DPM of interlobular bile ducts, associated with tubular dilatation of collecting renal tubules. Congenital hepatic fibrosis may derive from the same type of liver lesion, through a superimposed destructive type of cholangiopathy associated with scarring fibrosis. Caroli's disease represents DPM of the larger IHBDs, whereas Caroli's syndrome combines the lesions of Caroli's disease and congenital hepatic fibrosis. von Meyenburg complexes represent DPM of smaller interlobular ducts; their dilatation gives rise to the liver cysts in autosomal dominant polycystic kidney disease. Finally, DPM is a component of the tissue abnormalities in so-called mesenchymal hamartoma.

Pathogenesis of Ductal Plate Abnormalities

Intrahepatic bile ducts (IHBDs) develop from bi-potential liver progenitor cells in contact with themesenchyme of the portal vein and thus form the plates. The ductal plates are remodeled into mature tubular ducts. Lack of remodeling results in the persistence of periportal epithelial sleeves or plate malformation (DPM). A proposal is that virtually all congenital of IHBDs represent examples of DPM. Some early, severe types of extra-hepatic bile duct atresia are characterized by DPM, a suggestion of a prenatal beginning of the disease. Several congenital are characterized by dilatation of segments of IHBDs and variable degrees of fibrosis. Such fibrocystic diseases represent DPM at different levels of the biliary tree. Autosomal recessive polycystic kidney disease represents DPM of interlobular bile ducts, associated with tubular dilatation of collecting renal tubules. Congenital hepatic fibrosis may derive from the same type of liver lesion, through a superimposed destructive type of cholangiopathy associated with scarring fibrosis. Caroliapos;sdisease represents DPM of the larger IHBDs, whereas Caroliapos;s syndrome combines the lesions of Caroliapos;sdisease and congenital hepatic fibrosis, von Meyenburg complexes represent DPM of smaller interlobular ducts; their dilatation gives rise to the liver cysts in autosomal dominant polycystic kidney disease. Finally, DPM is a component of the tissue abnormalities in socalled mesenchymal hamartoma.

Serum chemistry of diseased English sole, Parophrys vetulus Girard, from polluted areas of Puget Sound, Washington

AbstractRelationships between selected serum components and microscopically detected idiopathic liver and kidney lesions were evaluated for English sole, Parophrys vetulus, sampled from urban and non‐urban areas of Puget Sound, Washington. The normal range for each serum chemistry parameter was based on English sole with no lesions, or sole with minor parasitic conditions or with lesions in tissues other than liver and kidney which did not significantly affect serum chemistry values. Generally, sole with idiopathic liver lesions had significantly different haematocrit values and levels of serum albumin, calcium, and bilirubin when compared to sole which were free of idiopathic liver and/or kidney lesions. Serum aspartate aminotransferase and alanine aminotransferase activity and serum levels of glucose, total protein, phosphates and magnesium were also abnormal, but appeared to be associated only with certain types of liver lesions. No association between kidney lesions and abnormal serum chemistry values was identified. In addition, English sole sampled from some urban areas of Puget Sound had particular serum chemistry values and liver lesion prevalences which were significantly different from those of sole sampled from non‐urban areas. These findings support the hypothesis that sole near highly urbanized environments may display other signs of impaired health in addition to high prevalences of tissue lesions. Thus, measurements of serum chemistry parameters, in conjunction with histopathological examination, appear to be useful techniques for evaluating the health of English sole.