Host Plant Type Research Articles

Combination of C3 and C4 host plants for the reproduction of the Sorowako indigenous arbuscular mycorrhizal fungi

The selection of the right host plant needs attention because of the interaction between the host plant, the type of AMF, the composition of the media, and the climate during its growth. It has been reported that in the C4 group, the best type of host plant used in producing AMF spores is Zea mays, while in the C3 group, Pueraria javanica is able to produce optimum AMF development, so a study is needed to combine C4 and C3 groups as hosts plant in AMF spore propagation. The study was arranged using a completely randomized design with 5 combination treatments of host plant groups C4 and C3, to determine the productivity of the host plant combination, the parameters observed were percentage of infected roots, abundance of mycorrhizal spores, and diameter of spore. The results showed that good host plant productivity was obtained from host plant combinations of Zea mays and Vigna angularis or Vigna unguiculata or Phaseolus vulgaris, where mycorrhizae can infect roots above 90% and produce spores that have a diameter of 300-400 µm, so it can be recommended that a host plant combination that has a fast life cycle is better used to stimulate the growth and regeneration of mycorrhizal spores, as a novelty in this study.

The Effect of a Host on the Primary Metabolic Profiling of Cuscuta Campestris' Main Organs, Haustoria, Stem and Flower.

Cuscuta campestris (dodder) is a stem holoparasitic plant without leaves or roots that parasitizes various types of host plants and causes damage to certain crops worldwide. This study aimed at gaining more knowledge about the effect of the hosts on the parasite’s levels of primary metabolites. To this end, metabolic profiling analyses were performed on the parasite’s three main organs, haustoria, stem and flowers, which developed on three hosts, Heliotropium hirsutissimum, Polygonum equisetiforme and Amaranthus viridis. The results showed significant differences in the metabolic profiles of C. campestris that developed on the different hosts, suggesting that the parasites rely highly on the host’s metabolites. However, changes in the metabolites’ contents between the organs that developed on the same host suggest that the parasite can also self-regulate its metabolites. Flowers, for example, have significantly higher levels of most of the amino acids and sugar acids, while haustoria and stem have higher levels of several sugars and polyols. Determination of total soluble proteins and phenolic compounds showed that the same pattern is detected in the organs unrelated to the hosts. This study contributes to our knowledge about the metabolic behavior of this parasite.

Comparative Study on Phytochemical Profile and Antioxidant Activity of an Epiphyte, Viscum album L. (White Berry Mistletoe), Derived from Different Host Trees.

The study aimed at evaluating the antioxidant profile of a medicinal epiphyte Viscum album L. harvested from three tree species, namely, Populus ciliata L, Ulmus villosa L., and Juglans regia L. The crude extracts were obtained with ethanol, methanol, and water and were evaluated for the total phenol content (TPC), total flavonoid content (TFC), and antioxidant activities using total reducing power (TRP), ferric reducing antioxidant power (FRAP), 1, 1-diphenyl 1-2-picryl-hydrazyl (DPPH), superoxide radical scavenging (SOR), and hydroxyl radical scavenging (•OH) assays. Our results showed that crude leaf extracts of plants harvested from the host Juglans regia L. exhibited higher yields of phytochemical constituents and noticeable antioxidative properties. The ethanolic leaf samples reported the highest phenols (13.46 ± 0.87 mg/g), flavonoids (2.38 ± 0.04 mg/g), FRAP (500.63 ± 12.58 μM Fe II/g DW), and DPPH (87.26% ± 0.30 mg/mL). Moreover, the highest values for TRP (4.24 ± 0.26 μg/mL), SOR (89.79% ± 0.73 mg/mL), and OH (67.16% ± 1.15 mg/mL) were obtained from aqueous leaf extracts. Further, Pearson correlation was used for quantifying the relationship between TPC, TFC, and antioxidant (FRAP, DPPH, SOR, OH) activities in Viscum album L. compared to their hosts. It was revealed that the epiphyte showed variation with the type of host plant and extracting solvent.

Invasion of a xeric forest by an exotic tree species in Argentina: Impacts on the diversity of arbuscular mycorrhizal fungi and pre-existing mutualistic relationships

This study aimed to analyse the effects of invasion by the exotic Ligustrum lucidum on mycorrhizal associations in forests of Celtis tala, and to determine the role that these fungi play in the invasion process. We analyzed colonization and response to mycorrhization of both plant species under greenhouse conditions after inoculation with soil obtained from patches of non-invaded native forests and invaded forests. The spores present in pots at the end of the experiment were counted and identified. Colonization of L. lucidum was greater in plants inoculated with invaded forest soil, whereas colonization of C. tala was greater in plants inoculated with non-invaded native forest soil. Principal component analysis of fungal species abundance showed differences in AMF composition according to host plant and forest type. The results show that L. lucidum establishes symbiosis with native AMF, which triggers changes in community structure. These changes favored the mycorrhization of L. lucidum and interfered with the mycorrhization of C. tala. AMF may have either a direct or indirect role in the invasion process, not only benefiting the invasive species but also preventing the regeneration of native plants.

Alteration in the Stylet Probing Behavior and Host Preference of the Vector Matsumuratettix hiroglyphicus (Hemiptera: Cicadellidae) After Infection with Sugarcane White Leaf Phytoplasma.

The leafhopper (Matsumuratettix hiroglyphicus (Matsumura) (Hemiptera: Cicadellidae)) is a crucial insect vector of the phytoplasma associated with sugarcane white leaf (SCWL) disease. The aim of this study was to compare the stylet probing behaviors of M. hiroglyphicus on healthy sugarcane plants, asymptomatic, and symptomatic SCWL-infected sugarcane plants, using DC electropenetrography. We also used host-selection preference (free-choice) assays to identify the preferred types of host plants, and scanning electron microscopy to observe stylet puncture holes and salivary flanges after leafhopper probing. According to a quantitative analysis of M. hiroglyphicus stylet probing, mean durations per insect of both phloem ingestion (waveform D; the phytoplasma-acquisition behavior) and phloem salivation (waveform C; the phytoplasma-inoculation behavior) were significantly longer on both types of infected sugarcane than on healthy plants. These longer overall durations were mainly because the same number of significantly longer-duration C and D events was performed on infected sugarcane compared with healthy plants. On free-choice tested plants, M. hiroglyphicus displayed a significantly greater preference to settle on the infected plants (both types) than the healthy sugarcane. These results provide the first empirical evidence that acquiring the SCWL phytoplasma alters the host selection and stylet probing behaviors of its main vector (M. hiroglyphicus). Our study thus contributes to a better understanding of the interactions between the insect vector and SCWL phytoplasma-infected plants, and will aid in developing novel disease management tactics for sugarcane.

Utilization of AMF and Essential Host Plants to Increase Growth of Sandalwood Seeds (Santalum album L.)

Sandalwood (Santalum album L.) is one of the native trees of Nusa Tenggara Timur Province because ithas high economic value and is the best endemic species in the world. The essential oil content in it has a very distinctive aroma and can be used to make various products such as handicrafts, wood carvings, incense, and oils for the perfume and cosmetics industry. Sandalwood is a semi parasitic plant whose life requires a host plant to obtain nutrients and water in the soil. There are many types of host plants that have been used, including casuarina equisetifolia, acacia mangium, sesbania grandiflora, alternanthera sp. and capsicum annum. In this research, sandalwood plants will be tested with the host plant Cymbopogon nardus, which from an economic point of view can provide benefits. Arbuscular Mycorrhizal Fungi (AMF) are a group of fungi from the phylum Glomeromycota which have mutualism symbiosis with higher plant root systems. AMF is able to survive on dry land. The purpose of this study was to analyze the effectiveness of AMF and the use of essential host plants to increase the growth of Sandalwood seeds in Timor Tengah Utara. In the parameter of high increase, the results of the Duncan test showed that the highest AMF treatment for agroforestry land and host chili plants was 19.01, which was different from AMF for agroforestry land within a host plant, Cymbopogon nardus namely 15.44. In the increase in diameter, the results of the Duncan test showed that the AMF treatment of agroforestry land and host chili plants had a significant effect on all treatments, namely 9.11. Duncan test results on plant biomass parameters showed that AMF treatment and host plants chili and lemongrass gave good treatment, namely 3.54 and 3.53 on shoot dry weight parameters and 3.75 and 3.42 root dry weight compared to control, namely 0.27, while at root and shoot ratio seed quality index, the seeds are suitable for use because they comply with the standard. In the parameters of root colonization, AMF and the host plants of chili and lemongrass were both able to adapt well, namely 56.0 and 54.0.

Effects of host plants on the population parameters and activities of detoxification enzymes in Laodelphax striatellus

AbstractThe small brown planthopper (SBPH), Laodelphax striatellus (Fallén) (Hemiptera: Delphacidae), is an economically important pest of rice (Oryza sativa L.), wheat (Triticum aestivum L.), and other members of the Poaceae. The effects of host plant type on SBPH life table parameters reared on two cultivars of rice (Zixiangnuo 2315 and Wuyujing 3), wheat (Jimai 22 and Nongda 26), and one grass weed (Echinochloa crusgalli Beauv.) under laboratory conditions were determined using the age‐stage, two‐sex life table. Additionally, the corresponding activities of three detoxifying enzymes – carboxylesterase (CarE), glutathione‐S‐transferase (GST), and mixed‐function oxidase (MFO) – were also measured. The results showed that host plant species and cultivars had effects on population parameters and activities of detoxification enzymes. Rice cv. Zixiangnuo 2315 and wheat cv. Nongda 26 were shown to be more suitable than the other three hosts. The activities of the three detoxifying enzymes showed different patterns of changes on the three host plants after feeding for 0–72 h. Regardless of short‐term (72 h) or long‐term (one generation) induction on host plants, the activity levels of GST and MFO in adult SBPH reared on rice ‘Zixiangnuo 2315’ were lower and higher, respectively, than on the other four hosts. The results may help understand the host adaptability of this pest and inform the development of strategies for effective field‐based integrated pest management programs.

Consequences of 'no-choice, fixed time' reciprocal host plant switches on nutrition and gut serine protease gene expression in Pieris brassicae L. (Lepidoptera: Pieridae).

Rapid adaptive responses were evident from reciprocal host-plant switches on performance, digestive physiology and relative gene expression of gut serine proteases in larvae of crucifer pest P. brassicae transferred from cauliflower (CF, Brassica oleracea var. botrytis, family Brassicaceae) to an alternate host, garden nasturtium, (GN, Tropaeolum majus L., family Tropaeolaceae) and vice-versa under laboratory conditions. Estimation of nutritional indices indicated that larvae of all instars tested consumed the least food and gained less weight on CF-GN diet (significant at p≤0.05) as compared to larvae feeding on CF-CF, GN-GN and GN-CF diets suggesting that the switch to GN was nutritionally less favorable for larval growth. Nevertheless, these larvae, especially fourth instars, were adroit in utilizing and digesting GN as a new host plant type. In vitro protease assays conducted to understand associated physiological responses within twelve hours indicated that levels and properties of gut proteases were significantly influenced by type of natal host-plant consumed, change in diet as well as larval age. Activities of gut trypsins and chymotrypsins in larvae feeding on CF-GN and GN-CF diets were distinct, and represented shifts toward profiles observed in larvae feeding continuously on GN-GN and CF-CF diets respectively. Results with diagnostic protease inhibitors like TLCK, STI and SBBI in these assays and gelatinolytic zymograms indicated complex and contrasting trends in gut serine protease activities in different instars from CF-GN diet versus GN-CF diet, likely due to ingestion of plant protease inhibitors present in the new diet. Cloning and sequencing of serine protease gene fragments expressed in gut tissues of fourth instar P. brassicae revealed diverse transcripts encoding putative trypsins and chymotrypsins belonging to at least ten lineages. Sequences of members of each lineage closely resembled lepidopteran serine protease orthologs including uncharacterized transcripts from Pieris rapae. Differential regulation of serine protease genes (Pbr1-Pbr5) was observed in larval guts of P. brassicae from CF-CF and GN-GN diets while expression of transcripts encoding two putative trypsins (Pbr3 and Pbr5) were significantly different in larvae from CF-GN and GN-CF diets. These results suggested that some gut serine proteases that were differentially expressed in larvae feeding on different species of host plants were also involved in rapid adaptations to dietary switches. A gene encoding nitrile-specifier protein (nsp) likely involved in detoxification of toxic products from interactions of ingested host plant glucosinolates with myrosinases was expressed to similar levels in these larvae. Taken together, these snapshots reflected contrasts in physiological and developmental plasticity of P. brassicae larvae to nutritional challenges from wide dietary switches in the short term and the prominent role of gut serine proteases in rapid dietary adaptations. This study may be useful in designing novel management strategies targeting candidate gut serine proteases of P. brassicae using RNA interference, gene editing or crops with transgenes encoding protease inhibitors from taxonomically-distant host plants.

Chemical convergence between a guild of facultative myrmecophilous caterpillars and host plants

Ants exert strong selective pressure on herbivorous insects, although some caterpillars can live in symbiosis with them using chemical defensive strategies. We investigated the adaptive resemblance of cuticular hydrocarbons (CHCs) in multitrophic systems involving a guild of facultative myrmecophilous caterpillar species (Lepidoptera: Lycaenidae), tending ants (Hymenoptera: Formicidae), and host plants from three families. We hypothesised that the CHCs of the caterpillars would resemble those of their host plants (chemical camouflage). We analysed CHCs using gas chromatography/mass spectrometry. Morisita's similarity index (SI) was used to compare CHC profiles of caterpillar species with different types of ant associations (commensal or mutualistic), ants, and host plants. We found strong convergence between caterpillars' CHCs and plants, especially for commensal species that do not provide secretion rewards for ants. Moreover, we found unexpected chemical convergence among mutualistic (trophobiotic) caterpillar species that offer caloric reward secretions to ants. These results show that the studied caterpillars acquire CHCs through their diet and that they vary according to host plant species and type of ant association (commensalism or mutualism). This ‘chemical camouflage’ of myrmecophilous caterpillars may have arisen as a defensive strategy allowing coexistence with ants on plants, whereas ‘chemical conspicuousness’ may have evolved in the context of honest signalling between mutualistic partners. We suggest the existence of chemical mimicry among myrmecophilous species, especially between mutualistic caterpillars. Cuticular chemical mixtures can play a key adaptive role in decreasing ant attacks and increasing caterpillar survival in multimodal sensory systems.

Genetic profiling of mistletoe (Viscum album L.) using RAPD-analysis

Aim. The aim of this research was genetic profiling and identification of genetic differences between V. album speciments, growing on deciduous and coniferous species of woody plants using RAPD markers. Methods. The method of polymerase chain reaction (PCR) with random primers (Random Amplification of Polymorphic DNA - RAPD) was used. Amplified DNA fragments were fractionated by electrophoresis in non-denaturing polyacrylamide gel. DNA bands were detected using the staining with silver nitrate. Results. All the studied mistletoe samples were differentiated from each other, and their unique molecular genetic profiles were obtained. 241 amplified DNA fragments were detected in the range from 200 to 2000 bp, 152 fragments (63%) were polymorphic. The samples were divided into two separate groups depending on the type of host plant. Conclusions. The fact that the samples formed two separate clades confirms the assumption that mistletoe, which grow on pine and grow on maple, represents two separate subspecies of V. album.
 Keywords: Viscum album L., molecular genetic markers, polymorphism, RAPD.

PENYEBARAN HAMA KUTU PUTIH PEPAYA PARACOCCUS MARGINATUS Williams & Granara de Willink (Hemiptera : Pseudococcidae) PADA BERBAGAI TANAMAN INANG DI KABUPATEN ENDE

Papaya is one of the many horticultural commodities grown by farmers. In mid-2008, thousands of trees in the District Sukaraga papaya, Bogor Regency pest infestation papaya white and whole tree has been attacked. P. marginatus is a prolific insect that attack several host plants, including tropical fruit crops and ornamental plants. This research aims to determine the spread of lice infestation in White Paracoccus marginatus Ende and type of host plants are attacked by pest Mite White Paracoccus marginatus. This study is useful to obtain information about the spread of pest P. marginatus in the district of Ende as a step in taking control measures, information to relevant agencies and farmer about the existence pf pest P. marginatus in Ende district, and continued research on the existence of natural enemies and pest bioecology O. Marginatus. Sampling study conducted in 21 sub-district in NTT Propinsis Ende. The study was conducted from March-August 2011. Materials used in this study plant pest Paracoccus marginatus, Alcohol 95%. The tools used are a collection of bottles, brushes, a microscope, transparent plastic, paper labels, Thermo-Hygrometer, coo box, stationery and literature. The result showe that the spread of pest P. marginatus was observed in 21 sub-district in Ende found only in the District 2 District East Ende and Ndona. Papaya is attacked by P. marginatus have chlorotic leaves, young leaves to fall and prospective fruits and fruit and leaves are covered by this pest by stacking layers of wax until the drought ends up dead. The intensity of damage caused by pest P. marginatus in the District of East Ende of 33.33% and 16.67% for Ndona District. The spread of pest P. marginatus in the district of Ende found at two locations namely Ende District East and District Ndona. Types of host plants are attacked by pest P. marginatus is Papaya plants.

IDENTIFIKASI HAMA PENGGEREK DAUN Liromiza spp. (Diptera: Agromyzidae) DAN PARASITOIDNYA YANG BERASOSIASI PADA BERBAGAI JENIS TANAMAN SAYURAN DI KECAMATAN DETUSOKO DAN KELIMUTU KABUPATEN ENDE

The aim of this research is to identify Liromiza spp. And the parasitoid is associated with various types of vegetable plants in Detusoko and Kelimutu Districts in Ende Regency. Data were analyzed by matching morphology of existing specimens with insect morphology books written by Bhorror 1970 and matching with standard specimens originating from Bali and Bogor. The results obtained from two vegetable producing districts in Ende, namely Detusoko and Kelimutu, show the following: There are two types of Liriomyza spp associated with several types of host plants in Ende Regency. L. huidobrensis (Diptera: Agromyzydae) is located in Kelimutu District and L Sativae (Diptera: Agromyzydae) is in Detusoko District. The diversity of parasitoids found in Detusoko Subdistrict were as many as four species from the Eulopidae family while in Kelimutu District there were five types of parasitoids originating from two families namely Braconidae and Euiopidae. Neosacharis okazaki is the most dominant type of parasitoid in each plant in Detusoko District. Opiits sp dominant in Kelimutu Subdistrict on the long bean, green beans, mustard greens, chicory and cabbage. While H. varicornis is dominant in Kelimutu District in tomato plants.

The Influence of Acacia Parasite Filtrate as Bioinsecticide for Bagworms (Methane Plana)

Dendrophthoe pentandra is a plant parasite that can be detrimental to the host plant and attacks various types of trees, such as acacia. The purpose of this study was to examine the effects and determine the concentration of acacia parasite filtrate on bagworms. This research was an experimental study which used 0%, 20%, 40%, 60%, 80%, and 100% parasite filtrate concentrations. The study employed the Completely Randomized Design method with ANOVA as the method of analysis. Based on the results of phytochemical tests, the parasite filtrate contained alkaloids, flavonoids, saponins, triterpenoids, phenolics, and tannins. The ANOVA test results showed that the concentration of acacia parasite filtrate affected the mortality of the bagworms. Furthermore, based on Duncan's test, the most effective concentrations to be applied were 80%, 60%, and 100%.

Population expansion and genomic adaptation to agricultural environments of the soybean looper, Chrysodeixis includens.

Evolutionary studies of insect pests improve our ability to anticipate problems in agricultural ecosystems, such as pest outbreaks, control failures, or expansions of the host range. Here, we investigated the mechanisms underlying the evolutionary processes behind the recent census size expansion and local adaptation of Chrysodeixis includens. First, we sequenced mitochondrial markers to conduct a phylogeographic investigation of C. includens historical processes. Then, we combined a de novo genotyping‐by‐sequencing approach with a study of agricultural landscapes to uncover recent processes of adaptation. Primarily, we found low genetic diversity across all markers and clear indications of a recent demographic expansion. We also found a lack of significant isolation by distance (IBD), and weak or absent genetic structure considering geographic locations. However, we did find initial signs of population differentiation that were associated with host plant types (i.e., soybean and cotton). Agricultural landscape attributes, including soybean crops, were significantly associated with putative markers under positive selection. Moreover, positive selection associated with host differentiation was putatively linked to digestive enzymes. This study showed how landscape composition and host plants can affect the evolutionary process of agricultural pest insects such as C. includens.

Natal-habitat experience mediates the relationship between insect and hostplant densities.

For some animals, the habitat which they first experience can influence the type of habitat which they select later in life and, thus, potentially their population distribution and dynamics. However, for many insect herbivores, whose natal habitat may consist of a single hostplant, the consequences of natal hostplant experience remain untested in landscapes relevant to the adult, which may select not only among plants, but among plant patches. As a first step towards understanding how natal hostplant experience shapes patterns of insect feeding damage in landscapes relevant to adults, we conducted partially caged field experiments with diamondback moths that were reared on either mustard or collard plants and then allowed to choose among and within patches of plants that varied in plant density and composition. We predicted that natal hostplant experience would interact with patch size and composition to influence the number of diamondback moth offspring and feeding damage per plant. As predicted, when moths were reared on collards, we found more offspring on and damage to collard plants in four-collard patches than in two-collard patches (i.e., resource concentration), but no difference when moths were reared on mustards. Contrary to predictions, we found no difference in the number of offspring on or damage to mixed plant patches compared with two- or four-collard plant patches regardless of natal hostplant type. Our research suggests that prior hostplant experience has complex consequences for how insects and their feeding damage are distributed in patchy environments and highlights the need for future research in this area.

Warming Effects on Periphyton Community and Abundance in Different Seasons Are Influenced by Nutrient State and Plant Type: A Shallow Lake Mesocosm Study.

Periphyton plays an important role in lake ecosystems processes, especially at low and intermediate nutrient levels where periphyton contribution to primary production can be similar to or exceed that of phytoplankton. Knowledge of how periphyton responds to key drivers such as climate change and nutrient enrichment is, therefore, crucial. We conducted a series of mesocosm experiments over four seasons to elucidate the responses of periphyton communities to nutrient (low and high, TN-0.33 mg L–1 TP-7.1 μg L–1 and TN-2.40 mg L–1 TP-165 μg L–1, respectively), temperature (ambient, IPCC A2 scenario and A2 + 50%) and plant type (two submerged macrophytes with different morphological structural complexity: Potamogeton crispus and Elodea canadensis, and their corresponding plastic imitations with similar size and structure). We found a noticeable seasonality in the abundance and composition of periphyton. In spring and summer, periphyton abundances were significantly higher in the turbid-high-nutrient state than in the clear-low-nutrient state, and in summer they were notably higher at ambient temperature than in climate scenario A2 and A2 + 50%. In contrast, periphyton abundances in autumn and winter were not influenced by nutrient and temperature, but they were notably higher on plants with a more complex morphological structure than simple ones. The genus composition of periphyton was significantly affected by nutrient–temperature interactions in all seasons and by plant type in winter. Moreover, periphyton functional composition exhibited noticeable seasonal change and responded strongly to nutrient enrichment and temperature rise in spring, summer, and autumn. Our results suggest that the effect of warming on periphyton abundance and composition in the different seasons varied with nutrient state and host plant type in these mesocosms, and similar results may likely be found under field conditions.

EFECTO DE LA ASOCIACIÓN DE Rhizobium etli - Phaseolus vulgaris L. SOBRE EL CRECIMIENTO VEGETAL Y LA PREFERENCIA DE Bemisia tabaci

<p><strong>Background.</strong> Cascading effects through tritrophic interactions (microorganisms, plants, herbivores) are basic components in ecosystems that are often influenced by force from bottom up or top down. One of the most important cases is the fixation of nitrogen through growth promoting bacteria of PGPR plants, which can alter the cascading effects in superior trophic interactions, through physical and chemical changes in the plants. Bacteria as growth promoters not only participate in growth, they are also considered the best biological control agents since they have the potential to suppress pathogenic microorganisms and induce systemic resistance against pest insects.<strong> Objective. </strong>This study aims to evaluate the contribution of the inoculation of the <em>Rhizobium etli </em>nitrogen fixing bacteria in the growth of <em>Phaseolus vulgaris</em> and the oviposition and preference of <em>Bemisia tabaci</em>. <strong>Methodology.</strong> Bean plants were established on sterilized soil, two batches of plants were formed: plants inoculated with <em>R. etli</em> and non-inoculated (control) plants. In the first experiment, were measured plant growth and characteristics related to defense against phytophages. In the second experiment, the preference and oviposition of <em>Bemisia tabaci</em> was evaluated by free choice and non-choice tests. <strong>Results.</strong> Inoculation with <em>R. etli</em> favored some specific growth characteristics, such as leaf area and root length. Phytophagous defense variables, as well as adult preference and oviposition of <em>B. tabaci</em> were not different between inoculated and non-inoculated plants.<strong> Implications. </strong>The main limitation of the use of PGPR depends on the type of soil and the variety of beans, so this inoculant could also be tested in cases where it has been proven that there is low natural colonization of <em>Rhizobium</em> in <em>Phaseolus lunatus </em>L. during its growth. <strong>Conclusion. </strong>Inoculation increased some growth variables; however, it did not affect the oviposition and preference of <em>Bemisia tabaci</em>. Additional work is required to assess the effects of factors, such as bean variety, synergy among rhizobacteria species, natural resistance of the host plant, environmental conditions and soil types.</p>

Quantity and transmission efficiency of an isolate of the Potato virus Y–Wilga (PVYN−Wi) by aphid species reared on different host plants

Potato virus Y (PVY) is a destructive plant virus causing important damage in different crops, particularly in potato. PVY is transmitted in a non-persistent manner by many aphid species, and the distal part of the stylet plays an important role in virus transmission by these aphids. Among other possible factors, the type of host plant on which the aphids develop could also affect virus transmission efficiency, yet little is known about this. Furthermore, the quantification of potyviruses in individual vectors has mainly focused on intact whole aphid body and not the stylet alone, whereas an even more precise indicator that could correlate with vector efficiency in non-persistently transmitted viruses is the quantity of the virus in the stylet. Therefore, this study aims to verify the effect of different host plants on which an aphid vector was reared on its ability to acquire and transmit the PVY, and whether the quantity of PVY in the aphid vector stylet could be used as a useful trait for evaluating vector efficiency. A key finding in this study indicates that when the aphid Myzus persicae was reared on broad bean and then exposed to acquire PVY, it transmitted PVY less efficiently (50%) compared to when it was reared on Brussels sprout (81%). Additionally, the transmission percentage correlated directly with the virus quantity in the stylet (r = 0.7) and not in the intact whole aphid body (r = 0.06). This could be exploited as a useful index for virus transmission efficiency in epidemiological studies or predictive schemes.

Pemanfaatan FMA dan Tanaman Inang untuk Meningkatkan Pertumbuhan Bibit Cendana (Santalum album Linn.)

The sandalwood tree (Santalum album Linn.) is an important tree species as well as a primadonna for the people of East Nusa Tenggara (NTT). It has high economic value for its aromatic wood and essential oil content that have a very distinctive aroma used to make various products such as handicrafts, woodcarvings, incense, and oil for the perfume and cosmetics industry. Sandalwood is a semi parasite plant that part of its life phase requires a host plant to get the nutrients and water. There are many types of host plants that have been used, among others, Casuarina equisetifolia, Acacia mangium, Terminalia microcarpa, Sesbania grandiflora, Alternanthera sp and Capsicum annum. In this research will be tested to try sandalwood planted with Cymbopogon nardus host plants, in terms of economics can provide benefits.Arbuscular mycorrhizal fungi (AMF) is a group of fungi from glomeromycota phylum that can symbiosis mutualism with root system of high level plant. The working principle of the mycorrhiza is to infect the root system of the host plant, producing intensive hyphae tissue so that the plant containing mycorrhiza will be able to increase the capacity in nutrient uptake. The utilization of host plants Alternanthera sp, Capsicum annum, and its application with AMF is the best solution to overcome the problem of developing sandalwood in TTU on the nursery. The purpose of this study was to analyze the effectiveness of AMF and utilization of the atsiri host plant to increase the growth of sandalwood seedlings in TTU. This study was designed using a complete random method (RAL) in split plot design. If the treatment has a significant effect then followed by Duncan Multiple Range Test (DMRT). Parameters observed were height (cm), number of leaf, diameter of sandalwood (mm), dry weight of root, seed quality index, ratio of root buds, and haustorium observation of Sandalwood, and also number of spore, root colonization and AMF dependency of Sandalwood.The results showed that the treatment of AMF with Capsicum annum host plant was 19.8 of high, number of leaf 18.9 on FMA treatment with host plant Capsicum annum, diameter of stem 2.24 mm on Alternanthera sp host treatments without AMF and 1.83 mm at AMF treatment with host plant Capsicum annum, dry weight of buds 2.00g on AMF treatment with Capsicum annum host plant, dry weight of roots AMF (M1) with alternanthera sp 0.70 g, root buds ratio of AMF with host plant alternanthera sp 4.05, seed quality index AMF with Alternanthera sp 4.16 and 82 % of root colonization on AMF with host plant Capsicum annum.Keywords: Santalum album Linn., AMF, host plant.

Spodoptera litura as a main pest in oil palm peat plantation

Nowadays oil palm plantation is attacked by Spodoptera litura especially in peat land plantation. This research was aimed to find out the types of host plants most preferred by larvae of S. litura. This research was conducted in 2 stages, the 1st stage used Randomized Block Design Factorial consisting of 2 factors, which was the origin of the larvae and the comodity with 16 combinations of treatments, the 2nd stage used Non Factorial Randomized Block Design with 2 treatment combinations, P1: Oil palm + Coconut + Sugar palm + Areca nut + Soybean + Green bean + Peanut+ Mucuna bracteata + 40 S. litura original from Oil palm, and P2: Oil palm + Coconut + Sugar palm + Areca nut + Soybean + Green bean + Peanut + Mucuna bracteata + 40 S. litura original from Soybean. The results showed that the preference of S. litura original from oil palm to the tested plants were Mucuna bracteata, Soybean, Green bean, Peanut, Oil palm, Areca nut, Sugar palm and Coconut. The preference of S. litura original from Soybean to the tested plants were Soybean, Mucuna bracteata, Peanut, Green bean, Oil palm, Areca nut, Coconut and Sugar palm.