Types Of Chains Research Articles

Development of the information quality scale for health information supply chain type 2 diabetes mellitus management using exploratory factor analysis.

Research on the quality scale of the healthcare supply chain is still limited. This study aimed to assess the information quality of the supply chain model with a focus on construct validity. Studies related to information quality measurement generally focus on measuring the dimensions of the completeness of medical records and consumer perspectives. We intended to assess the scale based on doctors needed as care coordinators on type 2 diabetes mellitus or the Non-Insulin-Dependent-Diabetes-Mellitus (NIDDM) program in primary healthcare. Sixty-four primary healthcare doctors with an age range of 24-51 years were involved in this research. The scale obtained was formed from the assessment of the point of view of a panel of experts through the content validity index (CVI). The exploratory factor analysis (EFA) method was used to explore the scale of information quality in the information supply chain model for the NIDDM chronic disease management program. The data analysis results indicated three main factors that affected the quality of the information supply chain model of NIDDM, namely accessibility, safety, and efficiency of information related to NIDDM. The results of the validity and reliability of the data showed that the scale used in this research was valid and reliable with a Cronbach alpha coefficient of 0.861. The scale developed in this research could be used to explore the quality of the information supply chain of NIDDM management in primary healthcare. Each item on the scale could explain the variables according to their respective groups.

In English

Boron carbide is a material of interest for personal body armor, but its low fracture toughness and amorphization limits its widespread use. Al and Si atoms in doped boron carbide reduce this problem. Passage of the substitution reaction in boron carbide powders with Al and Si vapors in vacuum was found. Certification methods: chemical analysis, full-profile XPA (Powder Cell for Windows. Version 2.4 FREE, W. Kraus & G. Nolze) and modeling in format of the 15-atomic unit cell B12(C-C-C) of trigonal syngony, spatial group R3 ̅m, Z = 3. A mixture of powders of boron carbide, aluminum or silicon is heat treated in vacuum at conventional evaporation temperatures of Al (1520 K) or Si (1640 K) for 1–5 h. The samples were purified with alkali and analyzed by arbitration chemical analysis for boron, carbon, aluminum and silicon. The formula composition of the input powders of boron carbide was determined as B12[(C-В-C)x(C-C-C)1-x], where x = 0.4–0.6. The aluminum substitution reaction takes place in both types of boron carbide chains and corresponds to the formula B12(C-Al-C) or AlB12C2. In the presence of silicon, the reaction took place exclusively at the positions of the tri-carbon chains. The composition of the obtained solid solution corresponds to - B12[(C-B-C)0.4(C-Si-C)0.6], starting powder B12[(C-B-C)0.4(C-C-C)0.6]. The absence of boron phases of silicide, such as SiB3 (SiB2.89), SiB6, SiBn (n ≈ 23) indicated greater resistance of C–B–C chains to interaction with vaporous Si. The content of Al and Si in the substituted phases is equal to 13.3 and 4.0 (% at.). Equivalent molar amounts of Al8B4C7 and SiC of gas-nano-phase origin were measured in the reaction products with vapor-like Al and Si. The area of tolerance chains of the boron carbide structure in the format of the average specific electronegativity (χN-Sh/rai) was found. It is in the range of values: 2.79 ≥ ССС ≥ СВС ≥ CSiC ≥ ВВС ≥ 2.18.

Nearly perfect spin-filtering effect with tunable spin-polarization direction and huge magnetoresistance in molecular spin valves

Spin-transport properties of molecular spin valves constructed by two chromium-salophene (CrSal) molecules connected with boron nitride (BN) atomic chains are investigated by applying a nonequiblium Green's function method with density functional theory. Nearly perfect spin-filtering effect can be found when the molecular spin valves are set in parallel magnetic configuration. In particular, the direction of the detected spin-polarization signal is reversible by tuning the types of BN atomic chains. Moreover, obvious magnetoresistance (MR) effects are also found in the molecular spin valves. More importantly, the MR ratio can be changed from about 359% to about 1.31×105% by adjusting the types of BN atomic chains. Our work provides a new avenue to realize high-performance multifunctional molecular spin valves.

Characterisation of the OTU domain deubiquitinase complement of Toxoplasma gondii.

The phylum Apicomplexa contains several parasitic species of medical and agricultural importance. The ubiquitination machinery remains, for the most part, uncharacterised in apicomplexan parasites, despite the important roles that it plays in eukaryotic biology. Bioinformatic analysis of the ubiquitination machinery in apicomplexan parasites revealed an expanded ovarian tumour domain-containing (OTU) deubiquitinase (DUB) family in Toxoplasma, potentially reflecting functional importance in apicomplexan parasites. This study presents comprehensive characterisation of Toxoplasma OTU DUBs. AlphaFold-guided structural analysis not only confirmed functional orthologues found across eukaryotes, but also identified apicomplexan-specific enzymes, subsequently enabling discovery of a cryptic OTU DUB in Plasmodium species. Comprehensive biochemical characterisation of 11 Toxoplasma OTU DUBs revealed activity against ubiquitin- and NEDD8-based substrates and revealed ubiquitin linkage preferences for Lys6-, Lys11-, Lys48-, and Lys63-linked chain types. We show that accessory domains in Toxoplasma OTU DUBs impose linkage preferences, and in case of apicomplexan-specific TgOTU9, we discover a cryptic ubiquitin-binding domain that is essential for TgOTU9 activity. Using the auxin-inducible degron (AID) to generate knockdown parasite lines, TgOTUD6B was found to be important for Toxoplasma growth.

One-Dimensional Chiral Copper Iodide Chain-Like Structure Cu4 I4 (R/S-3-quinuclidinol)3 with Near-Unity Photoluminescence Quantum Yield and Efficient Circularly Polarized Luminescence.

Chiral organic-inorganic hybrid metal halide materials have shown great potential for circularly polarized luminescence (CPL) related applications for their tunable structures and efficient emissions. Here, this work combines the highly emissive Cu4 I4 cubane cluster with chiral organic ligand R/S-3-quinuclidinol, to construct a new type of 1D Cu-I chains, namely Cu4 I4 (R/S-3-quinuclidinol)3 , crystallizing in noncentrosymmetric monoclinic P21 space group. These enantiomorphic hybrids exhibit long-term stability and show bright yellow emission with a photoluminescence quantum yield (PLQY) close to 100%. Due to the successful chirality transfer from the chiral ligands to the inorganic backbone, the enantiomers show intriguing chiroptical properties, such as circular dichroism (CD) and CPL. The CPL dissymmetry factor (glum ) is measured to be ≈4×10-3 . Time-resolved photoluminescence (PL) measurements show long averaged decay lifetime up to 10 µs. The structural details within the Cu4 I4 reveal the chiral nature of these basic building units, which are significantly different than in the achiral case. This discovery provides new structural insights for the design of high performance CPL materials and their applications in light emitting devices.

Fulfilling corporate social responsibility in a Closed-loop supply chain – Evidence from alternative remanufacturing models

As increasing awareness is being paid to the natural environment, corporates are now under scrutiny for their ecological footprints. Since the turn of the century, many of the world's leading transnational corporations started to implement close-loop supply chain to reduce emission while maintaining their competitive edges. With the urgent development of social responsibility, how will remanufacturing firms make the trade-offs among economic, environmental and social responsibility benefits? This study answers this question by discussing the effects of corporate social responsibility fulfilment in the two prevailing types of transnational closed-loop supply chain in developing countries: the original equipment manufacturer remanufacturing model in the exporting country and the OEM-authorized retailer remanufacturing model in the importing country. The results of this study inform researchers and practitioners on the balance of economic, environmental, and social obligations for transnational firms.

Стратегирование суверенитета отечественных брендов лёгкой промышленности и индустрии моды

A strategic analysis of the development of the domestic light industry and the fashion industry has been carried out. The article is based on the theory and methodology of strategizing developed and tested by the Center for Strategic Studies IMISS MSU, CEMI RAS, the Department of Economic and Financial Strategy of the MSE MSU, the Department of Strategy for Regional and Sectoral Development of the KemSU, the Department of Industrial Strategy of the National University of Science and Technology “MISIS” under the supervision of V.L. Kvint. The author proposes a system of strategic priorities aimed at strengthening the role of the industry in the economy, creating a federal technology transfer center for light industry and the fashion industry, a digital platform for the industry transfer of knowledge and experience, as well as clothing brands operating in the middle price segment. It is shown that the digital platform as a tool of the decision support system can establish a continuous transfer of knowledge and practical experience, and the technology transfer center can form a new type of cooperation chain for the development of the domestic market.

Analysis of transcriptome expression profiling data in oral leukoplakia and early and late‑stage oral squamous cell carcinoma.

The present study screened, potential prognostic biomarkers for oral carcinogenesis. The GSE85195 dataset, which consisted of oral leukoplakia (OL) and early and late-stage oral squamous cell carcinoma (OSCC) samples, was used. The differentially expressed genes (DEGs) in early OSCC vs. OL, late OSCC vs. OL and late OSCC vs. early OSCC groups were screened using the limma package in R. The Short Time-series Expression Miner software package was used to cluster DEGs with similar expression patterns in the course of disease progression (from OL to early and then late-stage OSCC). Moreover, the Database for Annotation, Visualization and Integrated Discovery online analysis tool was used to perform Gene Ontology functional annotation and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analysis. A protein-protein interaction (PPI) network was also constructed using the Search Tool for the Retrieval of Interacting Genes/Proteins database. Reverse transcription-quantitative PCR was performed to assess the mRNA expression levels of hub node genes in clinical samples, and receiver operating characteristic curve analysis was performed to assess the prognostic value of the hub genes. A total of 4,595, 6,042 and 2,738 DEGs were screened in the early OSCC vs. OL, late OSCC vs. OL and late OSCC vs. early OSCC groups, respectively. A total of 665 overlapping genes were identified when the screened DEGs were compared. Cluster 1 and cluster 7 were identified as the significant clusters, which contained 496 and 341 DEGs, respectively. A PPI network was constructed with 440 interaction pairs. There were five differentially expressed hub nodes identified in different stages from OL to OSCC. The results of the present study indicated that fibronectin 1, signal transducer and activator of transcription 1, collagen type II α1 chain, collagen type X α1 chain and collagen type IV α6 chain might serve as independent diagnostic factors for OL and OSCC, and as prognostic biomarkers for OL carcinogenesis.

Farm-to-School Programs in Portuguese Low-Density Rural Areas: How to Engage Farmers

Abstract Farm to School (FTS) programs is a type of Short Food Supply Chain (SFSC) that generate new economic opportunities for farmers while enhancing communities’ food security strategies, by improving the access to a safe, nutritious, and culturally acceptable diet, through a sustainable food system that maximizes self-reliance and social justice. However, the success of FTS is highly dependent on regional characteristics, and its implementation must deal with diverse views and opposing interests. In particular, farmers' willingness to engage in FTS may be affected by the need to adapt their practices to the demand of school canteens. The estimate of a logistic model is used in this study to analyse the factors that impact farmers' motivation to participate in FTS in low-density areas of Portugal, showing that market-oriented vegetable and fruit producers, living further away from the city are more likely to join FTS.

Pricing Decision Models of Manufacturer-Led Dual-Channel Supply Chain with Free-Rider Problem

We study the strategic pricing decision models of manufacture-led dual-channel supply chains with the free-rider problem under the service level and cost. We use the Stackelberg model to study the impact of the degree with the free-rider problem of consumers on the optimal pricing strategy and the optimal service level of the dual-channel supply chain under various decision-making modes and carry out a numerical simulation. The main conclusions are as follows: In the retailer’s dual-channel supply chain, the deepening of consumer free-riding behavior will reduce the enthusiasm of retailers, but the weak position of the channel will lead to improved service levels and reduced prices, as well as to increase the wholesale price to cover costs. In the manufacturer’s dual-channel supply chain, the deepening of consumer free-riding behavior will lead to a decline in the retailers’ service level and enthusiasm, as well as to a decrease in the wholesale prices and retailers’ pricing. In the two types of dual-channel supply chains, the demand of manufacturers’ network channels increases, the price increases first and then decreases, and the profits of all supply chain members decrease with the increase in the free-rider coefficient of consumers. Finally, we use numerical simulation to verify the validity of the above conclusions, which provides a scientific basis to make optimal pricing decisions in the manufacturer-led dual-channel supply chain.

Legionella longbeachae Regulates the Association of Polyubiquitinated Proteins on Bacterial Phagosome with Multiple Deubiquitinases.

Legionella spp. are the causative agents of a severe pneumonia known as Legionnaires' disease. Upon being engulfed by host cells, these environmental bacteria replicate intracellularly in a plasma membrane-derived niche termed Legionella-containing vacuole (LCV) in a way that requires the defective in organelle trafficking/intracellular multiplication (Dot/Icm) protein transporter. Our understanding of interactions between Legionella and its hosts was mostly based on studies of Legionella pneumophila. In this study, we found that the LCVs created by virulent Legionella longbeachae are similarly decorated by polyubiquitinated proteins to those formed by L. pneumophila and that the ubiquitin-proteasome system (UPS) is required for optimal intracellular growth of L. longbeachae. Furthermore, we utilized bioinformatics methods and the ubiquitin-vinylmethyl ester probe to obtain potential deubiquitinases (DUBs) encoded by L. longbeachae. These efforts led to the identification of 9 L. longbeachae DUBs that displayed distinct specificity toward ubiquitin chain types. Among these, LLO_1014 and LLO_2238 are associated with the LCVs and impact the accumulation of polyubiquitinated species on the bacterial phagosome. Moreover, LLO_1014 and LLO_2238 could fully restore the phenotypes associated with Δceg23 (lotB) and Δlem27 (lotC) mutants of L. pneumophila, indicating that these DUBs have similar functions. Together, these results reveal that L. longbeachae uses multiple DUBs to construct an intracellular niche for its replication. IMPORTANCE Legionella spp. are opportunistic intracellular bacterial pathogens that cause Legionnaires' disease. Legionella utilizes the Dot/Icm type IV secretion system to deliver effector protein into host cells to modulate various cellular functions. At least 26 L. pneumophila effectors are known to hijack the host ubiquitin system via diverse mechanisms. L. longbeachae is the second leading cause of Legionnaires' disease worldwide. However, our knowledge about the interactions between L. longbeachae and its hosts is very limited. Here, we found that, similar to L. pneumophila infection, the host ubiquitin proteasome system is also important for the intracellular replication of L. longbeachae. In addition, the bacterial phagosomes harboring L. longbeachae are enriched with polyubiquitinated proteins in a Dot/Icm system-dependent manner. We further identified 9 L. longbeachae proteins that function as DUBs with distinct ubiquitin chain specificity. Of note, several of the phagosome-associated L. longbeachae DUBs regulate the recruitment of polyubiquitinated proteins to the LCV.

Resistance training restores skeletal muscle atrophy and satellite cell content in an animal model of Alzheimer’s disease

Alzheimer’s disease (AD) is the most common neurodegenerative disease, and numerous recent findings suggest that several pathologic signs, including loss of muscle strength and mass, are also detected in these patients. In the present study, we evaluated muscle cross-sectional area (CSA), myonuclear number, satellite cell (SC) content, and myosin heavy chain (MyHC) types in an animal model of AD and examined the possible role of resistance training in controlling skeletal muscle size in this disease. Fifty-eight male rats were randomly divided into four groups: healthy-control (H-C), healthy-exercise (H-Ex), Alzheimer-control (A-C), and Alzheimer-exercise (A-Ex). AD was induced by the single injection of 1–42 amyloid into the CA1 region of the hippocampus (1 μl/site). The rats in H-Ex and A-Ex groups performed a 5-week resistance training period (17 sessions). The results indicated that AD induces significant skeletal muscle atrophy and reduces the myonuclear number and SC content in gastrocnemius muscle in both whole muscle cross-sections and isolated myofibers. Interestingly, we did not find any significant differences in the different MyHC distributions of AD animals compared with controls, while resistance training significantly increased the CSA of MyHC IIb fibers in both AD and healthy animals. Altogether, these observations suggest that the skeletal muscle of AD animals are more prone to atrophy and loss of myonuclear number and satellite cell content, while resistance training successfully restores these impairments.

On homological mirror symmetry for chain type polynomials

We consider Takahashi’s categorical interpretation of the Berglund–Hubsch mirror symmetry conjecture for invertible polynomials in the case of chain polynomials. Our strategy is based on a stronger claim that the relevant categories satisfy a recursion of directed \(A_{\infty }\)-categories, which may be of independent interest. We give a full proof of this claim on the B-side. On the A-side we give a detailed sketch of an argument, which falls short of a full proof because of certain missing foundational results in Fukaya–Seidel categories, most notably a generation statement.

Plantago Ovata Husk: An Investigation of Raw Aqueous Extracts. Osmotic, Hydrodynamic and Complex Rheological Characterisation

The aim of the study was to characterize raw aqueous extracts from Plantago ovata husk in terms of molecular chain mass, osmotic, hydrodynamic, and rheological properties. The raw extracts used in this study have not been yet investigated in the indicated research area. Determination of the molecular weight of the chains present in the extract was performed by gel permeation chromatography (GPC). Osmotic properties were characterized using membrane osmometry. Rheological properties were investigated via classical rotational rheology with normal force measurements, as well as less common but equally important measurements of extensional viscosity. Two types of chains with an average molecular mass of 200 and 1780 kDa were found. The values of the first virial coefficient (B2) indicate the predominance of biopolymer-biopolymer interactions. The hydrodynamic radius established at 25 and 30 °C was 74 and 67 nm, respectively, and lower than at 40 °C (>600 nm). The first critical concentration was determined: c*=0.11 g·dL-1. The dominance of negative normal force values resulting from the formation of a pseudo-gel structure of the heteroxylates was demonstrated. Extensional viscosity measurement results revealed that the studied extracts cannot be treated as simple shear-thinning fluids, as indicated by shear flow, but should be considered as viscoelastic fluids.

Utility of a Systematic Approach to Selecting Candidate Prodrugs: A Case Study Using Candesartan Ester Analogues

Development of prodrugs is a useful strategy to overcome some disadvantages of candidate drugs. Recently, we established a systematic approach to selecting appropriate prodrugs, and validated the utility of this approach using oseltamivir analogues. In this study, the utility of the approach was further examined using candesartan cilexetil and 20 kinds of its analogues having various types of side chain as model compounds. Log D values of analogues (2.5 to 4.7) were higher than that of candesartan (1.0), their active metabolite, and the results were reasonable for the purpose of improving permeability of candesartan. The analogues tended to be more soluble in artificial intestinal fluids than in artificial gastric fluid, owing to their acidic physicochemical characteristics. Their membrane permeabilities were not correlated with log D values, which can be attributed to the metabolism in Caco-2 cells used in this system. In human hepatocytes and enterocytes, 11 out of the 20 analogues were immediately hydrolyzed to candesartan, and species differences were observed in the hydrolysis efficiency. This study confirmed the utility of the systematic approach for selection of appropriate prodrugs that could be proceeded to in vivo pharmacokinetics study, with selection of suitable experimental animals.

Chains Stiffness Effect on the Vertical Segregation of Mixed Polymer Brushes in Selective Solvent.

The microstructure of the binary polymer brushes in the selective solvent was studied using the numerical lattice self-consisting field approach. The case was considered when the selectivity to the solvent (the Flory-Huggins parameter χ) was varied only for one type of chains (responsive chains) while the others (non-responsive chains) remained hydrophilic (χ = 0). In such a brush, with an increase in the hydrophobicity of the responsive chains, a transition occurs between two two-layer microstructures. In the initial state the ends of the longer responsive chains are located near the external surface of the brush and those of non-responsive chains are inside the brush. When the hydrophobicity of the responsive chains becomes high enough then the reversed two-layer microstructure is formed, when the ends of non-responsive chains are located near the brush surface and the responsive chains collapse on the brush bottom. In contrast to previous works, the stiffness parameter (Kuhn segment length p) for one or for both types of chains was varied and its effect on the mechanism and characteristics of the transition was studied. If the stiffness of only responsive chains increases, then the transition occurs with the formation of an intermediate three-layer microstructure, where a layer of responsive chains is located between layers formed by non-responsive ones. If both types of chains have the same p, then the transition occurs gradually without the formation of an intermediate three-layer microstructure. For both cases, the effect of p on the critical value of χ*, corresponding to the transition point and on the steepness of the transition was investigated.

Salvia miltiorrhiza polysaccharide and its related metabolite 5-methoxyindole-3-carboxaldehyde ameliorate experimental colitis by regulating Nrf2/Keap1 signaling pathway

The roots of Salvia miltiorrhiza have been used in Traditional Chinese Medicine for thousands of years. However, tons of aerial parts of this plant are usually discarded in the production of roots preparation. To make better use of these plant resources, the polysaccharide isolated from the aerial part of S. miltiorrhiza was investigated for its potential protection against intestinal diseases. A pectic polysaccharide (SMAP-1) was isolated and characterized being composed of homogalacturonan as the main chain and rhamnogalacturonan type I as ramified region, with side chains including arabinans and possible arabinogalactan type I and II. SMAP-1 exhibited robust protective effects against dextran sodium sulfate (DSS)-induced colitis and restored colitis symptoms, colonic inflammation, and barrier functions. Anti-oxidative effects were also observed by up-regulating Nrf2/Keap1 signaling pathway. Additionally, the level of serum 5-methoxyindole-3-carboxaldehyde (5-MC) was restored by SMAP-1 identified in metabolomic analysis, being correlated with the aforementioned effects. Protection against oxidative stress on intestinal porcine enterocyte cells (IPEC-J2) by 5-MC was observed through the activation of Nrf2/Keap1 system, as also shown by SMAP-1. In conclusion, SMAP-1 could be a promising candidate for colitis prevention, and 5-MC could be the signal metabolite of SMAP-1 in protecting against oxidative stress in the intestine.

Exploring Steroidal Surfactants as Potential Drug Carriers for an Anticancer Drug Curcumin: An Insight into the Effect of Surfactants' Structure on the Photophysical Properties, Stability, and Activity of Curcumin.

Despite having tremendous medicinal benefits, the practical applications of curcumin are limited, owing to two major challenges: poor aqueous solubility and lack of bioavailability. In this regard, biosurfactant-based micellar systems have surged recently for the development of novel and more effective formulations because of their biological relevance. This study deals with a comprehensive and comparative investigation on the effect of seven structurally different steroidal surfactants on the photophysical properties of curcumin and also evaluates these steroidal surfactants as possible drug delivery media for curcumin. The photophysical properties of curcumin exhibited a strong dependence on the structure of the steroidal surfactant; the extent of excited-state proton transfer between curcumin and the surfactants depends strongly on the type of the side chain in the surfactants, which mostly dictates the photophysics of curcumin in the presence of these structural variants. The solubility of curcumin and its stability at different pHs and temperatures and in the presence of salt are significantly enhanced in the presence of these surfactants. Furthermore, the curcumin-loaded micelles exhibited improved intracellular uptake and cytotoxicity against MCF-7 cancer cells than pristine curcumin. Among these steroidal surfactants, CHAPS, the zwitterionic derivative of cholic acid, was the most efficient one to offer better solubility and stability to curcumin under all conditions, and the death rate of MCF-7 cells by curcumin was found to be the highest in the presence of CHAPS, indicating the enhanced bioavailability of curcumin. Therefore, CHAPS-based colloids are found to be promising candidates as potential drug carriers for curcumin.

Serological proteome analysis identifies crustacean myosin heavy chain type 1 protein and house dust mite Der p 14 as cross-reacting allergens.

Allergies to house dust mite (HDM) and to crustaceans are clinically and pathogenically linked. Several homologous allergenic proteins have been identified, among which tropomyosin is the prototype, expressing epitopes endowed with variable levels of immunoglobulin E (IgE) cross-reactivity. Component-resolved diagnosis (CRD) does not allow a thorough characterization of all relevant IgE reactivities to these allergen sources. We studied 1 patient allergic to shrimp with positive skin prick test to HDM and negative scores for IgE to HDM allergen components routinely used in CRD (group 1 and 2 allergens, Der p 23 and tropomyosin). In order to identify the allergen(s) involved in IgE reactivity, we used serological proteome analysis (SERPA), which utilizes two-dimensional gel electrophoresis (2DE), immunoblotting and mass spectrometry (MS). The identified allergenic proteins were tested with sera from 20 crustacean-allergic patients and 19 grass-allergic patients serving as controls. Der p 14 and myosin heavy chain type 1 (MHC1) were identified as the components recognized by patient's IgE in the proteome of Dermatophagoides pteronyssinus and Penaeus monodon, respectively. The MHC1 protein shows about 30% sequence identity with Der p 14 in specific domains, and cross-reactivity against epitopes shared by the 2 proteins was demonstrated by reduced reactivity to shrimp extract following pre-incubation with Der p 14. Serum IgE from 5 out of 20 patients allergic to crustaceans reacted with MHC1, compared to none among 19 controls (p < 0.05). We identified MHC1 as a relevant allergic component in the proteome of Penaeus monodon, the prototypic allergen source used in diagnosis of allergy to crustaceans. Our data demonstrate MHC1 cross-reactivity between MHC1 and Der p 14 from Dermatophagoides pteronyssinus.

Chemically Acylated tRNAs are Functional in Zebrafish Embryos.

Genetic code expansion has pushed protein chemistry past the canonical 22 amino acids. The key enzymes that make this possible are engineered aminoacyl tRNA synthetases. However, as the number of genetically encoded amino acids has increased over the years, obvious limits in the type and size of novel side chains that can be accommodated by the synthetase enzyme become apparent. Here, we show that chemically acylating tRNAs allow for robust, site-specific incorporation of unnatural amino acids into proteins in zebrafish embryos, an important model organism for human health and development. We apply this approach to incorporate a unique photocaged histidine analogue for which synthetase engineering efforts have failed. Additionally, we demonstrate optical control over different enzymes in live embryos by installing photocaged histidine into their active sites.