Holotype Research Articles

Optimal selection of specimens for metagenomic next-generation sequencing in diagnosing periprosthetic joint infections.

This study aimed to assess the diagnostic value of metagenomic next-generation sequencing (mNGS) across synovial fluid, prosthetic sonicate fluid, and periprosthetic tissues among patients with periprosthetic joint infection (PJI), intending to optimize specimen selection for mNGS in these patients. This prospective study involved 61 patients undergoing revision arthroplasty between September 2021 and September 2022 at the First Affiliated Hospital of Zhengzhou University. Among them, 43 cases were diagnosed as PJI, and 18 as aseptic loosening (AL) based on the American Musculoskeletal Infection Society (MSIS) criteria. Preoperative or intraoperative synovial fluid, periprosthetic tissues, and prosthetic sonicate fluid were collected, each divided into two portions for mNGS and culture. Comparative analyses were conducted between the microbiological results and diagnostic efficacy derived from mNGS and culture tests. Furthermore, the variability in mNGS diagnostic efficacy for PJI across different specimen types was assessed. The sensitivity and specificity of mNGS diagnosis was 93% and 94.4% for all types of PJI specimens; the sensitivity and specificity of culture diagnosis was 72.1% and 100%, respectively. The diagnostic sensitivity of mNGS was significantly higher than that of culture (X2 = 6.541, P=0.011), with no statistically significant difference in specificity (X2 = 1.029, P=0.310). The sensitivity of the synovial fluid was 83.7% and the specificity was 94.4%; the sensitivity of the prosthetic sonicate fluid was 90.7% and the specificity was 94.4%; and the sensitivity of the periprosthetic tissue was 81.4% and the specificity was 100%. Notably, the mNGS of prosthetic sonicate fluid displayed a superior pathogen detection rate compared to other specimen types. mNGS can function as a precise diagnostic tool for identifying pathogens in PJI patients using three types of specimens. Due to its superior ability in pathogen identification, prosthetic sonicate fluid can replace synovial fluid and periprosthetic tissue as the optimal sample choice for mNGS.

Description of Discocotyle ciray n. sp. (Monogenea: Discocotylidae) from Parahucho perryi (Brevoort) from Hokkaido, Japan, with a redescription of D. sagittata (Leuckart, 1842).

Discocotyle sagittata (Leuckart, 1842) (Monogenea: Discocotylidae) is redescribed, based on specimens collected from the type host, Salmo trutta Linnaeus, from the type locality, Freiburg, Germany, supplemented with specimens from S. trutta and rainbow trout, Oncorhynchus mykiss (Walbaum) reared in an Austrian aquarium. The diagnosis of the genus Discocotyle Diesing, 1850 is emended. Discocotyle ciray n. sp. is described, based on immature, preadult and adult specimens from the salmonid, Parahucho perryi (Brevoort) at Eniwa, Hokkaido, Japan. Adult specimens of the new species were about twice as large as those of D. sagittata from S. trutta. When the type specimens of D. ciray n. sp. were examined together with museum specimens from P. perryi at Tsurui, Hokkaido, the body and clamp sizes were positively correlated to the host size. Their measurements from a smaller P. perryi at Tsurui overlapped with those of D. sagittata, showing that these size differences were not suitable differentiating keys. Discocotyle ciray n. sp. can be separated from D. sagittata by the morphologies of the female genital system (relatively anteriorly positioned ovary, short joint vaginal duct and much more strongly winding uterus). The genetic distances of COI mtDNA sequence between D. ciray n. sp. and D. sagittata were 18.0-18.6%. These remarkable genetic divergences also supported the distinct taxonomic status of D. ciray n. sp.

From caves to seamounts: the hidden diversity of tetractinellid sponges from the Balearic Islands, with the description of eight new species

The sponge fauna of the Western Mediterranean stands as one of the most studied in the world. Yet sampling new habitats and a poorly studied region like the Balearic Islands highlights once again our limited knowledge of this group of animals. This work focused on demosponges of the order Tetractinellida collected in several research surveys (2016–2021) on a variety of ecosystems of the Balearic Islands, including shallow caves, seamounts and trawl fishing grounds, in a broad depth range (0–725 m). Tetractinellid material from the North Atlantic and more than twenty type specimens were also examined and, for some, re-described in this work. All species were barcoded with the traditional molecular markers COI (Folmer fragment) and 28S (C1-C2 or C1-D2 fragment). A total of 36 species were identified, mostly belonging to the family Geodiidae (15 species), thereby bringing the number of tetractinellids recorded in the Balearic Islands from 15 to 39. Eight species from this study are new: Stelletta mortarium sp. nov., Penares cavernensis sp. nov., Penares isabellae sp. nov., Geodia bibilonae sp. nov., Geodia microsphaera sp. nov. and Geodia matrix sp. nov. from the Balearic Islands; Geodia phlegraeioides sp. nov. and Caminus xavierae sp. nov. from the North East Atlantic. Stelletta dichoclada and Erylus corsicus are reported for the first time since their description in Corsica in 1983. Pachastrella ovisternata is documented for the first time in the Mediterranean Sea. Finally, after comparisons of type material, we propose new synonymies: Geodia anceps as a junior synonym of Geodia geodina, Erylus cantabricus as a junior synonym of Erylus discophorus and Spongosorites maximus as a junior synonym of Characella pachastrelloides.

Finite element simulation of axial crushing of flat and C-channel composite coupons using a modified mesomodel

The present article investigates the axial crushing behavior of IM7/8552 material system. Two types of specimens are considered-(a) flat coupons with saw-tooth trigger and (b) C-channels with 45° chamfer as a trigger. All the crushing test data (load, displacement and velocity history curves) is shared in the Crashworthiness working group (CWG) consortium of the Composite material handbook (CMH17.org). Flat crush coupons are tested at the University of Utah and the C-channels are tested at the John H. Glenn Research Center of the National aeronautics and space administration (NASA). Three types of layups are considered for flat coupons: QI1-[90/±45/0]2S, HL2-[902/02/±45/02] and HL3-[90/45/02/90/-45/02]. Two types of layups are considered for C-channels: HL2-[902/02/±45/02] and HL3-[90/45/02/90/-45/02]. Both the specimens are subjected to crush loading with an initial velocity and lumped mass attached to the impactor. All the ply and interface material properties come from the current authors' testing campaign except for strength and stiffness in the fiber direction. The crushing test cases are simulated in LS-Dyna software. Ply and interface behavior is described via user-defined material subroutines of LS-Dyna software. Each ply is modeled using 3D solid elements and the interfaces are modeled explicitly using cohesive elements. Intraply behavior is described using a novel modified meso model proposed by the present authors. Intraply model accounts for nonlinear damage, plasticity, ply fracture toughness and finite element mesh size. Interply response is modeled using bi-linear law. Predictions from present FE models showed a good correlation with crush test data. The effect of finite element model parameters e.g., filtering frequency, mesh size, interface properties, ply fracture toughness, element erosion criterion, contact stiffness and friction coefficient on crushing predictions is demonstrated by conducting parametric studies.

The first harvestman described from Bocas del Toro Archipelago: a new Poecilaemula (Opiliones: Cosmetidae) and notes about the genus

ABSTRACT The discovery of a new Cosmetidae species from Bocas del Toro Archipelago, Panama, with unusual sexual dimorphism and colouration, presented an identification challenge due to the limited knowledge of the taxonomy of cosmetids in Central America. The new species is notable for the extensive bright orange blots on the dorsal scutum and by the unusual structure of tibia IV of males, which is not incrassate, and bears two compact rows of strong spines. After examination of both type and non-type specimens of the type species of the genus Poecilaemula Roewer, 1912 (Meterginus signatus Banks, 1909), we decided that the new species should be assigned to this genus, and that the current circumscription of the genus is debatable as a natural group. Here, we propose a new definition of Poecilaemula, which includes three species: the type species from Costa Rica; Poecilaemula iching sp. n. from western Panama; and the species Poecilaemula eutypa (Chamberlin, 1925) comb. n. from eastern Panama, which is newly transferred from Paecilaema C.L. Koch, 1839. Additionally, Paecilaemana reimoseri Roewer, 1933 is considered a new junior subjective synonym of Meterginus signatus Banks 1909 (currently under Poecilaemula). The five South American species currently included in Poecilaemula reveal a geographic discrepancy, and they should be transferred to other genera in future investigations because they do not share any special similarity with the type species of the genus. http://www.zoobank.org/urn:lsid:zoobank.org:pub:0101B1EE-CF5F-4781-96FF-E797C71CF05C

Ki-67 Testing in Breast Cancer: Assessing Variability With Scoring Methods and Specimen Types and the Potential Subsequent Impact on Therapy Eligibility.

Abemaciclib was originally FDA approved for patients with ER-positive/HER2-negative breast cancer with Ki-67 expression ≥20%. However, there were no guidelines provided on which specimen to test or which scoring method to use. We performed a comprehensive study evaluating the variation in Ki-67 expression in breast specimens from 50 consecutive patients who could have been eligible for abemaciclib therapy. Three pathologists with breast expertise each performed a blinded review with 3 different manual scoring methods [estimated (EST), unweighted (UNW), and weighted (WT) (WT recommended by the International Ki-67 in Breast Cancer Working Group)]. Quantitative image analysis (QIA) using the HALO platform was also performed. Three different specimen types [core needle biopsy (CNB) (n=63), resection (RES) (n=52), and axillary lymph node metastasis (ALN) (n=50)] were evaluated for each patient. The average Ki-67 for all specimens was 14.68% for EST, 14.46% for UNW, 14.15% for WT, and 11.15% for QIA. For the manual methods, the range between the lowest and highest Ki-67 for each specimen between the 3 pathologists was 8.44 for EST, 5.94 for WT, and 5.93 for UNW. The WT method limited interobserver variability with ICC1=0.959 (EST ICC1=0.922 and UNW=0.949). Using the aforementioned cutoff of Ki-67 ≥20% versus <20% to determine treatment eligibility, the averaged EST method yields 20 of 50 patients (40%) who would have been treatment-eligible, versus 15 (30%) for the UNW, 17 (34%) for the WT, and 12 (24%) for the QIA. There was no statistically significant difference in Ki-67 among the 3 specimen types. The average Ki-67 difference was 4.36 for CNB vs RES, 6.95 for CNB versus ALN, and RES versus ALN (P=0.93, 0.99, and 0.94, respectively). Our study concludes that further refinement in Ki-67 scoring is advisable to reduce clinically significant variation.

Authentic specimens of Juncaceae names stored in the Herbarium of Ivan Franko National University of Lviv

Background. The Herbarium of Ivan Franko National University of Lviv (LW) is one of the oldest herbaria in Ukraine. It was established in 1783 by Prof. B. Schiwerek. At present, its holdings are estimated at 300,000 specimens, including about 2000 type ones. Scientific processing of herbarium collections, their digitization, and cataloging are in progress. In 2002, the Herbarium of Ivan Franko National University of Lviv was included in the State Register of Scientific Objects of Ukraine that have the national heritage status. The aim of this study was to compile a list of Juncaceae type specimens of the Herbarium LW. Material and Methods. In the Herbarium LW, specimens of Juncaceae were analyzed. The authentic specimens of other herbaria were studied on JSTOR Global Plants. Using SimpleMappr, we created a map of loci classici of the type specimens names housed in this herbarium. Acronyms of herbaria are given according to the Index Herbariorum. Results. In the Herbarium of Ivan Franko National University of Lviv, we found authentic specimens of six names: Juncus bulbosus var. salinus Schur, Juncus czetzii Schur, Juncus kotschyi Boiss., Juncus lampocarpus var. viviparus Schur, Juncus transsilvanicus Schur, Luzula alpigena Schur. For each name, we provide: 1) nomenclatural citation, 2) information from the protologue, 3) the text of the herbarium label of the type specimen kept in the Herbarium LW, 4) herbarium specimen number, 5) notes. Most of the authentic specimens are kept in F. Schur’s nominative collection. Conclusions. Six authentic specimens of Juncaceae are kept in the Herbarium LW: one isolectotype and 5 syntypes. Collectors of type specimens are F. Schur and T. Kotschy. The geographic areas of the Juncaceae type collection include 2 countries: Romania and Iran. At present, all of these names belong to synonyms.

Utility of quantitative loop mediated isothermal amplification (qLAMP) assay for the diagnosis of invasive pneumococcal disease (IPD)

PurposeQuantitative LAMP (qLAMP) assay is one of the recent and emerging diagnostic tests for infectious diseases. Only a few studies exist comparing this assay with quantitative real-time PCR (qPCR) for the diagnosis of invasive pneumococcal disease (IPD). AimTo compare the diagnostic performance of qLAMP assay with qPCR targeting autolysin gene for the diagnosis of invasive pneumococcal disease. MethodsNinety six blood samples and 73 CSF samples from patients clinically suspected with community acquired pneumonia and acute meningitis were tested by qPCR and qLAMP assays using previously published primers and protocols. The qPCR was considered as the gold standard test and the diagnostic performance was assessed by calculating sensitivity, specificity, positive and negative predictive values, and kappa coefficient for the level of agreement between the tests. Chi-squared/Fisher exact test was used to compare categorical variables (positive/negative). ResultsThirty two blood samples and 22 CSF samples were positive by qPCR while 24 and 20 samples were positive by qLAMP assay respectively. The sensitivity of qLAMP assay was only 86.4% and 75% when tested on CSF and blood samples respectively. However, the qLAMP assay was in substantial to almost perfect agreement when compared with qPCR. The results were statistically significant in both sample types (P < 0.001). ConclusionsThe performance of qLAMP assay can vary based on the specimen type. It has very high specificity and had substantial to almost perfect agreement, and thus may be an alternative to qPCR for the diagnosis of IPD.

An illustrated catalog of Disteniidae (Coleoptera: Chrysomeloidea) type specimens deposited at the Museu de Zoologia da Universidade de São Paulo

The type specimens of the family Disteniidae Thomson, 1861 (Coleoptera) housed at the Museu de Zoologia, Universidade de São Paulo (MZSP) are cataloged and illustrated. Photos of dorsal and ventral habitus, labels, and complete label data of all the specimens are provided. There are 147 specimens belonging to 70 names: 33 primary types, 31 holotypes, and two lectotypes; and 114 secondary types, 52 paratypes, and two paralectotypes. Elytrimitatrix (Grossifemora) Santos-Silva &amp; Hovore, 2007 is the genus with the greatest number of species with type material, 21 species, followed by Cometes Lepeletier &amp; Audinet-Serville, 1828 and Novantinoe Santos-Silva &amp; Hovore, 2007 with 15 and 13 species, respectively.

Consistency in human papillomavirus type detection between self-collected vaginal specimens and physician-sampled cervical specimens.

With the rising need for accessible cervical cancer screening, self-sampling methods offer a promising alternative to traditional physician-led sampling. This study aims to evaluate the efficacy of the HygeiaTouch Self Sampling Kit for Women in detecting human papillomavirus (HPV) types and predicting cervical lesions. We studied the concordance in identifying high-risk HPV (hrHPV) types between samples collected by physicians and those self-collected by women using a self-sampling kit for validation. Women aged 21-65, fitting into specific categories based on their cervical health history were eligible. Cohen's kappa coefficient to gauge concordance between the two specimen types and relative accuracy metrics in identifying cervical intraepithelial neoplasia (CIN) were also calculated, with physician-sampled specimens serving as a reference. A total of 1210 participants from three institutes were involved. The self-sampling kit closely matched the physician-led method in terms of collecting valid specimens (100% vs. 100%), identifying hrHPV types (kappa: 0.75, 95% confidence interval [95% CI]: 0.72-0.79; agreement: 87.7%, 95% CI: 85.8-89.6) and predicting CIN grade 2 or worse (CIN2+) (relative sensitivity: 0.949, relative accuracy: 0.959). Kappa values varied between 0.71 and 0.83 for different hrHPV types and combinations, with an overall value 0.75 (95% CI: 0.72-0.79) signifying robust compatibility between the two methods. Our study underscores the potential of the HygeiaTouch Self Sampling Kit as a reliable, efficient, and user-friendly alternative to traditional sampling methods. This suggests that self-sampling could be pivotal in expanding cervical cancer screening accessibility and enhancing detection rates.

Fracture toughness determination for epoxy-based polymer concrete mixtures: Applicability of different rectangular beam and circular disc specimens

The purpose of this study was to examine the potential impact of the testing procedure, the shape of the test sample, loading method and sample size on the KIc value of polymer concrete (PC) materials. The research involved experimental investigations using five different testing techniques and specimen types, namely the single edge notched beam (SENB), short bend beam (SBB), semi-circular bend (SCB), edge notch disc bend (ENDB), and center cracked Brazilian disc (CCBD). A typical PC mixture made of mineral silicious aggregate, ML506 epoxy resin, chopped E-glass, and foundry sand filler. Despite the difference in the shape and loading type of the tested samples, the KIc data obtained from all groups of specimens are in good agreement with together and with the SENB proposed by RILEM. Depending on the test type, the KIc value varied from 1.43 to 1.74 MPa.m0.5 and the discrepancy between the data was mainly attributed to the type of loading (compression or bending) and the crack type (center crack or edge crack). The T-stress affects the fracture toughness for different testing samples and configurations. The lowest fracture toughness corresponds to the CCBD specimen (the center cracked disc loaded diametrically). The other test samples with edge cracks and loaded by a three-point bend setup showed KIc = 1.7 - 1.74 MPa.m0.5. Moreover, the fracture toughness data for PC mixtures can be achieved by utilizing sub-sized samples like SBB (for smaller amounts of PC material) instead of larger beam samples (i.e., SENB).

Unearthing the identity, taxonomy and nomenclature of the enigmatic Marchantiopsis stoloniscyphula (Marchantiophyta, Marchantiaceae) from China

AbstractOwing to a combination of reportedly unique morphological characters and an unlocatable type specimen, Marchantiopsis stoloniscyphula has been regarded as an enigmatic species in Marchantiaceae since its description, and all subsequent taxonomic treatments of this species were made without examination of the type. We located the original material of M. stoloniscyphula which was included as an admixture within the holotype of Wiesnerella fasciaria deposited in IFP. Our morphological examination found that M. stoloniscyphula and W. fasciaria are conspecific with Marchantia papillata subsp. grossibarba and Sandea japonica, respectively. The nomenclature of Marchantiopsis and M. stoloniscyphula are discussed. Lectotypes for M. stoloniscyphula and W. fasciaria are also designated.

Nomenclatural updates in the sedge-flora of western Uttar Pradesh

Establishing all plant’s correct identity and nomenclature is crucial in the current context. Therefore, an updated list and nomenclature status of sedges of western Uttar Pradesh, India has been provided in this article. This research paper incorporates extensive consultation of taxonomic databases, examination of type specimens and scrutiny of published works, including Ph.D thesis, research papers, books, and floras. To ensure the accuracy of binomial nomenclature, a systematic approach is adopted, starting with the utilization of search engines to cross-verify and validate taxonomic information. This includes a rigorous examination of online databases and repositories to access up-to-date information on botanical nomenclature. This paper aims to provideaccepted binomials with correct author citations of the sedges growing in western Uttar Pradesh because several names in the published literature are not accepted now. Such publications include several synonyms as accepted name, as well as mistakes in authority. A pivotal aspect of this work involves the verification of binomial identities through the examination of protologues and type specimens. Keeping this in mind, a comprehensive study for solving and updating nomenclature issues hasbeen carried out using different databases, websites and recent publications. We updated the nomenclature of all sedges of western Uttar Pradesh included in publications after 1959. Review of literature and field studies shows that western Uttar Pradesh includes a total of 91 species belonging to 14 genera published in 18 publications. In this study, 25 names of past publications were corrected. This is the first report on updated name changes in the floristic component of western Uttar Pradesh, where 41 names of sedges have been updated. This research paper covers the accepted name, synonym, taxonomic treatment, and typification.

Low-high-low cyclic performance of screw connections for fibre-polymer composites

This study examines the cyclic performance of screw connections for joining fibre polymer composite members. Cyclic tensile experiments were conducted on connection specimens consisting of a flat panel (FP) and a square hollow section (SHS) at a right angle. A low-high-low (LHL) cyclic loading program was employed, consisting of seven varying-amplitude loading sequences spanning over 10,361 cycles, in order to simulate wind actions according to relevant standards. Four types of specimens, connected using screws or/and adhesive bonding, were examined, under three load amplitude levels corresponding to 50%, 80%, or 100% of the monotonic ultimate load. All specimens loaded under the LHL amplitude levels of 50% and 80% of the monotonic ultimate load survived after all LHL cycles. These survived specimens were subsequently loaded to determine their retention capacity. The results revealed that no less than 89% of the monotonic strength was preserved. In the LHL loading process, the specimens with adhesive bonding showed more severe degradation of stiffness than their screw-only counterparts, due to the initiation of delamination near the bonded side edges on the FP in the early stage of cycles, with further propagation as the number of cycles increased. The screw-only specimens also demonstrated comparable retention capacity in comparison to the adhesively-bonded specimens.

A new species of Pseudorientalia (Gastropoda: Hydrobiidae) from midwestern Trkiye with notes on Pseudorientalia natolica (Kster, 1853).

The members of the genus Pseudorientalia Radoman, 1983 are small hydrobiid species with characteristic features of the penis and female genitalia. The type locality of the genus is the southern Marmara region, northwestern Trkiye. In this study, sampling was carried out in the upper Sakarya River Basin, western-middle Anatolia to obtain hydrobiid specimens, which yielded a new Pseudorientalia. The shell, penis, and female genitalia of the new species are described. The measurement of some shell characters is compared with the other representatives of the genus from Trkiye, northern Greece, and some Aegean islands: Samos, Chios and Lesvos. In addition, notes on Pseudorientalia natolica are also given from examination of the type specimens.

Cercospora polygonatum, a new species causing gray leaf spot disease in Polygonatum cyrtonema.

This study identified a new species (Cercospora Polygonatum) that causes gray leaf spot (GLS) disease in cultivated Polygonatum cyrtonema. This fungal species was isolated from the affected region of GLS on P. cyrtonema leaves. Pathogenicity bioassays were conducted based on Koch's postulates. Morphology was examined based on the features of conidiomata, conidiogenous loci, conidia/conidiophores, and conidiogenous cells. The rDNA internal transcribed spacer region, calmodulin, translation elongation factor 1-alpha, and histone genes were subjected to phylogenetic analysis using MrBayes tool via in Phylosuite. Bootstrap support analysis for phylogenetic placement confirmed the new species, which was significantly different from the closely related species C. senecionis-walkeri and C. zeae-maydis. The morphological characteristics also supported this finding, with the conidiogenous of C. polygonatum being considerably shorter than those of C. senecionis-walkeri or C. zeae-maydis. In addition, C. polygonatum was distinguished by its cultural characteristics. As this fungus was isolated from P. cyrtonema, it was named C. polygonatum F.Q. Yin, M. Liu&W. L. Ma, sp. nov. The type specimen (H8-2) was preserved at the China General Microbiological Culture Collection Center. This is the first report of GLS caused by C. polygonatum on P. cyrtonema leaves in China. The current study enriches the knowledge regarding Cercospora sp., contributes to the identification of a species causing GLS in P. cyrtonema, and provides useful information for the effective management of this disease.

Experimental Study of Novel Demountable Shear Connectors for Steel-concrete Composite Buildings

Sustainable composite structures in building construction are assembled using demountable structural elements that can be reused in the circular economy. The current research and development project, in cooperation with Budapest University of Technology and Economics and KÉSZ Group, bim.GROUP Ltd., Hungary, aims to design a novel demountable steel-concrete composite slab and frame system for buildings. The key component of this construction is the demountable shear connector. In the current research, novel bolted shear connectors with embedded bolts and threaded rods are developed and studied that can fit the applied technology of the industrial partner. One of the leading aspects of this connection is the consideration of bolt hole clearance, since it occurs initial slip and stiffness reduction of the composite beam. In the first phase of the research program, demountable and economical structural details were developed, which can reduce the stiffness reduction with the proper resistance and ductility features. To study the behavior of these shear connections, a push-out experimental program was designed and completed in March and April 2023. It is observed that novel shear connectors have a proper behavior with sufficient resistance and ductility, which is applicable according to the Eurocode 4 standard and fits the objectives of the research and development project. In the paper, the developed structural details and the push-out experimental program are presented with general results and statements besides a detailed evaluation of a specified specimen type.

Post-Wear Surface Morphology Assessment of Selective Laser Melting (SLM) AlSi10Mg Specimens after Heat Exposure to Different Gas Flames

The wear surface morphology of AlSi10Mg specimens, originally manufactured using selective laser melting (SLM), has been analyzed in the context of exposure to heat from gas flames. The first stage of the experimental work included the performance of surface heat-exposure on SLM-prepared specimens through oxyacetylene gas welding. Gas welding was utilized with three different flames, namely; reducing, neutral, and oxidizing on the as-built specimens of SLM. The post-surface-treated specimens were subjected to pin-on-disk wear testing against fixed parameters. After the performance of wear testing at two different radii, the mass loss of each of the four types of specimens was calculated including the three specimens exposed to heat along with the as-built specimens. The results showed that the maximum amount of mass losses at 24 mm and 30 mm radii belongs to the neutral flame specimens and the least was for the as-built condition specimens. Upon analysis, the heat-exposure specimens through all three types of gas flames resulted in an increase in the amount of mass in contrast to the as-built specimens. Moreover, the morphologies of the developed wear tracks at surfaces were examined using the scanning electron microscope (SEM) for the understating of the mechanism.

Clarification of the identity and geographic distribution of Phyllanthus claussenii and typification of two names in Phyllanthaceae (Phyllanthoideae)

Phyllanthus claussenii is one of the most frequently mentioned species of Phyllanthus sect. Phyllanthus subsect. Clausseniani in various studies. However, it is often mistaken for P. subemarginatus in most collections. In the present work, we present a clarification of the identity and geographic distribution of P. claussenii and the typification of Phyllanthus acuminatus and Savia sessiliflora. The proposal of lectotypes for P. acuminatus and S. sessiliflora will enable the accurate identification of the species and avoid potential ambiguity. To achieve this, protologues and type specimens, including digital images, were analyzed, along with specimens from collections of national and international herbaria.

New method for determining the mode I fracture toughness of shotcrete: edge-notched partial disc test

The fracture toughness (mode I) of shotcrete specimens was obtained using edge-notched partial disc (ENPD) specimens. Notched Brazilian discs (NBDs) were also tested to validate the results of the ENPD experiments. Numerical analysis was also conducted on the ENPD results to compare the measured and numerically obtained fracture toughness values. The notch lengths in the ENPD specimens were 15, 30, 45 and 60 mm, while the notch lengths in the NBD specimens were 10, 20, 30, 40, 50 and 60 mm. It was found that the flat joint model accurately predicted the potential crack growth path and crack initiation stress as compared with the experimental results. It was also deduced that the fracture toughness was roughly the same with an increase in the notch length. The tensile strength (σt) and fracture toughness (KIC) of the shotcrete specimens were found to be meaningfully correlated (σt = 7.92KIC). The ENPD tests yielded the lowest fracture toughness values because of the pure tensile stress distribution on the failure surface. It was also found that the derived fracture extension patterns from the laboratory investigations were in acceptable agreement with the outputs of numerical simulations.