Types Of Plants Research Articles

Study on Remediation of Cd Contamination in Riverbed Soils Around Outfalls by Six Typical Plants

Study on Remediation of Cd Contamination in Riverbed Soils Around Outfalls by Six Typical Plants

Leaf extract of Kentut (Paederia foetida L.) as a preventive measure against interleukin-6 expression in the liver of mice in a sepsis model injected with Escherichia coli

Kentut leaves (Paederia foetida L.) are a type of medicinal plant that can be used as a preventative medicine against sepsis. This plant contains secondary metabolite compounds such as alkaloids, flavonoids, triterpenoids, saponins, and other active compounds. This research aims to determine the influence and effective dosage of Kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in a sepsis model injected with E. coli. The method used was a Completely Randomized Design (CRD). The study involved 24 white male mice divided into 6 groups. Data analysis was performed using One Way ANOVA. The average values of IL-6 expression in the mouse livers for each group are as follows: KN at 7.09%±0.06; K+ at 26.36%±0.02; K- at 72.60%±0.05; PI (100mg/kgBW) at 71.04%±0.04; PII (300mg/kgBW) at 62.22%±0.02; and PIII (500mg/kgBW) at 40.92%±0.01. The research results indicate that there is an influence of kentut leaf extract as a preventive measure against IL-6 expression in the livers of mice in the sepsis model injected with E. coli, with a significance value of 0.000 or p-value 0.005. The effective dosage of kentut leaf extract as a preventive measure against IL-6 expression is the PIII dosage of 500mg/kgBW. The anti-inflammatory mechanism in sepsis is thought to be caused by the presence of flavonoids, alkaloids, phenolic acids, and terpenoid compounds. The most likely anti-inflammatory mechanism is believed to involve flavonoids inhibiting cyclooxygenase (COX) and lipoxygenase (LOX) enzymes involved in the synthesis of inflammatory mediators such as prostaglandins and leukotrienes, which can trigger IL-6 production.

Identifying High‐Yielding and Drought‐Tolerant Wheat Cultivars Based on Ideotypic Traits and Yield Responses to Stress

ABSTRACTDrought is one of the most adverse factors affecting plant growth and productivity. Identifying elite genotypes and their ideotypic traits conferring high yield potential and drought tolerance is critical in selecting and breeding drought‐tolerant wheat cultivars. In this study, we conducted field experiments at the Xinxiang Agricultural Comprehensive Experimental station in the North China Plain from 2018 to 2020 and assessed 209 wheat cultivars released since the 1940s under irrigated and nonirrigated conditions. Then, we selected drought‐tolerant cultivars by classifying them into four groups based on yield response to drought stress and several drought indices. Finally, the key ideotypic traits associated with high yield potential and drought tolerance were identified. Results indicated that the grain yield of the 209 cultivars decreased on average by 10.4% and 9.4% under nonirrigated treatment in 2018–2019 and 2019–2020, respectively, relative to full irrigation. The high‐yielding cultivars under both irrigation treatments are characterised by a compact plant type, larger thousand‐grain weight, larger chlorophyll content, higher leaf photosynthesis, shorter plant height and stay‐green traits. The stomatal and nonstomatal limitations are strongly associated with genotype yield performance, elucidating a potential mechanism underlying drought tolerance. Drought tolerance and yield stability of wheat cultivars have been improved through breeding over the past 70 years. Our findings enhance understanding of drought tolerance and identify genotypes and traits beneficial for breeding high‐yielding and drought‐tolerant cultivars.

2-Cinnamamido-4-Methylpentanamide and N-(2-Hydroxypropanoyl)Cinnamamide: Synthesis, Characterization, and Molecular Docking Studies Through PBP2a Protein

ABSTRACT. Cinnamic acid has been found in several types of plants and has a diverse spectrum of bioactivity. Derivatization of cinnamic acid related to the improving bioactivity of a compound. Cinnamic acid can be found as an acid or in conjugated form with amides, esters, aldehydes. This research focus on the synthesis of amide derivatives of cinnamic acid to improve the bioactivity. Two compounds namely 2-cinnamamido-4-methylpentanamide (1a) and N-(2-hydroxypropanoyl)cinnamamide (1b) were carried out through amidation reaction using carbodiimide coupling reagent for 24 hours. The synthesized target compounds were characterized using FT-IR, GCMS-MS, 1H-NMR and 13C-NMR spectroscopy. The compounds were evaluated their antibacterial activity by molecular docking simulation against PBP2a (PDB ID: 1MWT) using AutoDock4 and AutoDockTools software. PBP2a is one of the main proteins in MRSA that has evolved to resist β-lactam antibiotics and was proposed to be the most likely target of MRSA. The inhibitory process of this protein works through inhibition of the bacterial cell wall. The synthesis process of 1a and 1b produced yields of 22.13% and 25.20%. Molecular docking results showed that 1a and 1b had better energy (ΔGbinding -7.33 and -6.29 kcal/mol) than Streptomycin as the control positive. The compounds of 1a and 1b had interactions on PBP2a through hydrogen bond with Asn464, Thr600, and Tyr519. This present study showed that the synthesized compounds from cinnamic acid derivatives have a potential to be used as antibacterial agents. Keywords: Amidation, antibacterial, 2-cinnamamido-4-methylpentanamide, molecular docking, N-(2-hydroxypropanoyl) cinnamamide.

Modeling the Efficiency of Biogas Plants by Using an Interval Data Analysis Method

This article considers the task of developing mathematical models and their computer implementation that would establish the dependence of pH (acidity of the environment) on the volume and structure of raw materials for daily loading, as well as on the operating parameters of temperature and humidity based on the interval analysis of experimental data obtained during BGP research of a given type. In the process of research, based on the developed interval models, it was established that this indicator depends on the volume and structure of raw materials, as well as on the temperature and humidity of the substrate in the bioreactor. To build this mathematical model, it is proposed to use the method of interval data analysis and the method of identification of model parameters based on multidimensional optimization. The results of experimental studies for a specific type of biogas plant are given, and interval models with guaranteed prognostic properties that characterize the pH of the environment depending on the specific type of bio-raw material of solid and liquid fractions, temperature, and humidity are obtained. Based on the use of different types of raw materials, the developed models, based on experimental data, describe different configurations of the structure and volumes of raw materials for daily loading. The obtained mathematical models are an algebraic nonlinear equation that can be applied to control the level of pH of the environment in the bioreactor by determining the optimal volumes of raw materials of each type during the loading period depending on the temperature and humidity of the substrate in the bioreactor.

A new chemometrically optimised HPLC method for the simultaneous determination of imatinib mesylate and capecitabine, the active medicinal components

ABSTRACT Anticancer drugs such as imatinib mesylate (IMP) and capecitabine (CAP) and their metabolites enter the water cycle through wastewater treatment plant effluents or directly into the water cycle due to their limited biodegradability in typical wastewater treatment plants. However, their long-term effects on water resources are unknown due to their toxicity. The hazards concerned with chronic exposure to these substances raise concerns about their impact on organisms, environmental safety and biodiversity conservation. Therefore, in this study, a chemometrically optimised HPLC method was developed for the simultaneous determination of trace amounts of cytotoxic agents IMP and CAP used in cancer treatment in environmental water, synthetic urine samples and drug samples. Using the Box-Behnken design, optimum conditions discovered using the traditional method were optimised. The effect of each element was evaluated in the design by four factors based on HPLC determination. For the examined levels, mobile phase 1, mobile phase 2 ratio, flow rate, and column temperature parameters for the HPLC method were determined. Using regression analysis on the experimental results, the factors influencing resolution were identified. To achieve the reliability of the results, the Analysis of Variance (ANOVA) test was used. The ANOVA test was employed to verify the consistency of the data. With the information gathered, a model was developed. HPLC was used to examine the target chemicals. A good linearity (R2 > 0.99) was established for the IMP and CAP in the concentration ranges of 1.18–23.59 µg mL−1 and 0.76–15.09 µg mL−1, respectively, under optimum circumstances. The recoveries for spiked samples at three concentration levels ranged from 98.31 to 101.11. The created method has been applied to synthetic urine samples, regional environmental water samples, and pharmaceutical samples with success.

Phytoconstituents with cardioprotective properties: A pharmacological overview on their efficacy against myocardial infarction.

Myocardial infarction (MI) is considered one of the most common cardiac diseases and major cause of death worldwide. The prevalence of MI and MI-associated mortality have been increasing in recent years due to poor lifestyle habits viz. residency, obesity, stress, and pollution. Synthetic drugs for the treatment of MI provide good chance of survival; however, the demand to search more safe, effective, and natural drugs is increasing. Plants provide fruitful sources for powerful antioxidant and anti-inflammatory agents for prevention and/or treatment of MI. However, many plant extracts lack exact information about their possible dosage, toxicity and drug interactions which may hinder their usefulness as potential treatment options. Phytoconstituents play cardioprotective role by either acting as a prophylactic or adjuvant therapy to the concurrently used synthetic drugs to decrease the dosage or relief the side effects of such drugs. This review highlights the role of different herbal formulations, examples of plant extracts and types of several isolated phytoconstituents (phenolic acids, flavonoids, stilbenes, alkaloids, phenyl propanoids) in the prevention of MI with reported activities. Moreover, their possible mechanisms of action are also discussed to guide future research for the development of safer substitutes to manage MI.

Sustainable Business Development Strategy In Cassava Cracker Production Business

Cassava is a plant originating from Central and South America, as a type of tuber plant that has high carbohydrates. As many people know, food made from cassava will give a feeling of satiety, so in some regions in Indonesia cassava is used as a staple food as a substitute for rice. As a root crop that cannot last long, some people take the initiative to process cassava into dry food that can be stored for several months but still has a good taste as a snack or food accompaniment. Several groups of housewives took the initiative to process cassava into savory and crispy crackers, and can increase family income. As a cassava cracker processor in the countryside, it still uses a chopper for raw materials that are still simple to produce cassava crackers. The purpose of this study is to find out the level of effectiveness of cassava cracker processing so that it has more selling value and export quality, with a strategy that can develop a sustainable business, so that it becomes one of the products that is competitive and exported abroad. In terms of production, cassava cracker orders are quite promising, but are still constrained by several conditions that result in not fulfilling all orders. This makes the researcher intend to help producers to be able to increase the production of cassava crackers that have characteristics and can be competitive until they are exported abroad. The strategies offered will have an impact on improving business results and sustainable and competitive cracker production processes, testing the quality of cassava crackers, packaging and branding, and marketing strategies. The expected output is that researchers can contribute to sustainable business development for producers and can be published in international journals.

The GATA transcription factor BcWCL2 regulates citric acid secretion to maintain redox homeostasis and full virulence in Botrytis cinerea.

Botrytis cinerea is a typical necrotrophic plant pathogenic fungus which can deliberately acidify host tissues and trigger oxidative bursts therein to facilitate its virulence. The white collar complex (WCC), consisting of BcWCL1 and BcWCL2, is recognized as the primary light receptor in B. cinerea. Nevertheless, the specific mechanisms through which the WCC components, particularly BcWCL2 as a GATA transcription factor, control virulence are not yet fully understood. This study demonstrates that deletion of BcWCL2 results in the loss of light-sensitive phenotypic characteristics. Additionally, the Δbcwcl2 strain exhibits reduced secretion of citrate, delayed infection cushion development, weaker hyphal penetration, and decreased virulence. The application of exogenous citric acid was found to restore infection cushion formation, hyphal penetration, and virulence of the Δbcwcl2 strain. Transcriptome analysis at 48 h post-inoculation revealed that two citrate synthases, putative citrate transporters, hydrolytic enzymes, and reactive oxygen species scavenging-related genes were down-regulated in Δbcwcl2, whereas exogenous citric acid application restored the expression of the above genes involved in the early infection process of Δbcwcl2. Moreover, the expression of Bcvel1, a known regulator of citrate secretion, tissue acidification, and secondary metabolism, was down-regulated in Δbcwcl2 but not in Δbcwcl1. ChIP-qPCR and electrophoretic mobility shift assays revealed that BcWCL2 can bind to the promoter sequences of Bcvel1. Overexpressing Bcvel1 in Δbcwcl2 was found to rescue the mutant defects. Collectively, our findings indicate that BcWCL2 regulates the expression of the global regulator Bcvel1 to influence citrate secretion, tissue acidification, redox homeostasis, and virulence of B. cinerea.IMPORTANCEThis study illustrated the significance of the fungal blue light receptor component BcWCL2 protein in regulating citrate secretion in Botrytis cinerea. Unlike BcWCL1, BcWCL2 may contribute to redox homeostasis maintenance during infection cushion formation, ultimately proving to be essential for full virulence. It is also demonstrated that BcWCL2 can regulate the expression of Bcvel1 to influence host tissue acidification, citrate secretion, infection cushion development, and virulence. While the role of organic acids secreted by plant pathogenic fungi in fungus-host interactions has been recognized, this paper revealed the importance, regulatory mechanisms, and key transcription factors that control organic acid secretion. These understanding of the pathogenetic mechanism of plant pathogens can provide valuable insights for developing effective prevention and treatment strategies against fungal diseases.

Prevalence of Snails and Farmers' Practice Towards the Management of Giant African Snail, Achatina fulica Bowdich, 1822 in Kapilvastu, Nepal

The Giant African Snail (GAS), Achatina fulica Bowdich, 1822, is the world's largest, most damaging, and highly invasive land snail, capable of consuming about 10% of its body weight daily and affecting at least 500 types of plants. Despite the high incidence of this pest, management options are limited and ineffective. In this context, this study was carried out to assess overall snail distribution, damage and management practices adopted by farmers against GAS. A household survey of randomly selected 60 households was carried out using semi-structured questionnaires at Banganga Municipality-01, Kapilvastu, Nepal. The results revealed the prevalence of three different snails: the GAS, Achatina fulica Bowdich, 1822, the Common snail, Indoplanorbis exustus (Deshayes, 1834), and the Paddy Snail or Ghonghi or Ghongi, Pila globose (Swainson, 1822) in moist areas, drains, and grassy lands, among them, GAS causing the most significant damage during the rainy season. The most vulnerable stage of crops to GAS was the vegetative stage of development (53.3%) in vegetables and fruits, with the most preferred crop family being the Cucurbitaceae (45%). The majority of respondents (48%) believed that the lack of effective control measures was the main reason for the growing infestation and extent of damage. The 'Hand-picking and killing' method was considered the most effective and suitable method in the farming community over other control measures. It has been reported that the snail problem has increased over the years, and very little effort has been made by the concerned authorities to manage this pest. In conclusion, snails, especially GAS, pose major challenges to agricultural production in Nepal. Therefore, appropriate and cost-effective management strategies are required for their sustainable management.

Influence of residential physical environments on occupant experience: considering the moderating effects of indoor plants and climate zones

PurposeHome is a place/system/product that becomes increasingly occupied with various tasks used to be performed in workplaces. However, the knowledge of the relationship between residential physical environments and occupant experience is limited, especially when considering the effect of indoor plants (IPs) and climate zones. To address the gap, this study conducted a questionnaire survey in three cities across different regions in China.Design/methodology/approachBased on User Experience and Customer Satisfaction Index theory, following the research paradigm, a total of 627 valid samples were collected and analyzed in a stepwise statistical analysis, including descriptive statistics, reliability and validity test, correlation test and region comparison, then the model of PROCESS was adopted to examine the hypotheses that are given based on the former studies.FindingsThe results showed that residential physical environments have a significant effect on occupant satisfaction (OS) in all regions, as well as OS on occupant performance. However, regional differences were found that OS is a complete mediator in the Middle region, while a partial mediator in the North and South. A slight moderating effect of IPs was also found in the region of South. Nevertheless, both the number of plants and plant types have a significant moderating effect on the mechanism.Originality/valueBesides combining two theories and confirming the mechanism in the residential physical environment, it is also the first study to consider the moderating effects of IPs and climate zones, providing potential empirical support for not only design and management stages but also facing global challenges of working at home and climate changes.

Formate-tetrahydrofolate ligase: supplying the cytosolic one-carbon network in roots with one-carbon units originating from glycolate.

The metabolism of tetrahydrofolate (H4PteGlun)-bound one-carbon (C1) units (C1 metabolism) is multifaceted and required for plant growth, but it is unclear what of many possible synthesis pathways provide C1 units in specific organelles and tissues. One possible source of C1 units is via formate-tetrahydrofolate ligase, which catalyzes the reversible ATP-driven production of 10-formyltetrahydrofolate (10-formyl-H4PteGlun) from formate and tetrahydrofolate (H4PteGlun). Here, we report biochemical and functional characterization of the enzyme from Arabidopsis thaliana (AtFTHFL). We show that the recombinant AtFTHFL has lower Km and kcat values with pentaglutamyl tetrahydrofolate (H4PteGlu5) as compared to monoglutamyl tetrahydrofolate (H4PteGlu1), resulting in virtually identical catalytic efficiencies for the two substrates. Stable transformation of Arabidopsis plants with the EGFP-tagged AtFTHFL, followed with fluorescence microscopy, demonstrated cytosolic signal. Two independent T-DNA insertion lines with impaired AtFTHFL function had shorter roots compared to the wild type plants, demonstrating the importance of this enzyme for root growth. Overexpressing AtFTHFL led to the accumulation of H4PteGlun + 5,10-methylene-H4PteGlun and serine, accompanied with the depletion of formate and glycolate, in roots of the transgenic Arabidopsis plants. This metabolic adjustment supports the hypothesis that AtFTHFL feeds the cytosolic C1 network in roots with C1 units originating from glycolate, and that these units are then used mainly for biosynthesis of serine, and not as much for the biosynthesis of 5-methyl-H4PteGlun, methionine, and S-adenosylmethionine. This finding has implications for any future attempts to engineer one-carbon unit-requiring products through manipulation of the one-carbon metabolic network in non-photosynthetic organs.

Assessment of sustainability of natural complexes of the Minyarsky physiographic region of the Western slope of the Southern Urals

The article considers the evaluation of sustainability of natural complexes of low-mountain sections of the western slope of the Southern Urals (Minyarsk physical and geographical area) on the basis of analysis of landscape profiles on the indicator of sustainability of natural complexes. Peculiarities of the study area are highlighted: specific climatic conditions, complex geological and geomorphological conditions with ongoing industrial development, as well as a variety of soil and hydrological conditions combined with anthropogenic transformation of the territory. In the course of landscape profiling, as well as the application of methods of assessment of anthropogenic transformation of landscapes (at the level of the terrain), a decrease of diversity in natural complexes on the studied slopes was established, Replacement of typical plant communities by secondary successional communities in lowland areas dominated by horsetail. It has also been established that the aggregate of conditions in natural complexes indicates anthropogenic transformation of slope landscapes, the aggregate leading factor of which is the geochemical position on the element of the slope, Low resistance to external influence of the soil with a rinsing type of water regime defining plant associations. The obtained materials can be used as a basis for analysis of ecological condition of landscapes of the western slope of the Southern Urals, as well as characteristics of slope types of locations. Materials allow to further assess the recreational attractiveness of landscapes and the choice of ways of natural use of the studied area.

Agro-technological aspects of growing branched annual apple nursery plants

The article presents an analytical review of Russian publications reporting the results of studies that search for optimal ways to affect apple plants in order to obtain branched annual plants. Since it has been proved that nursery plants of a certain age and quality are preferable for each type of intensive planting, as their use promotes the early maturity of trees thus ensuring a quick economic return on capital investments, it is currently relevant to study the production of apple tree planting material with specified biometric parameters. The use of mechanical impact on the above-ground part of grafted plants to suppress apical dominance is considered; the effectiveness of stimulating premature shoots in the crown area is confirmed. Much attention is paid by researchers to the chemical treatments of plants in the fields of fruit tree nurseries to improve the quality of annual plants. The use of cytokinin preparations and gibberellic acids is shown to be highly effective. The maximum effect on obtaining branched annual plants in most works was achieved in pomological apple varieties that are prone to the formation of premature shoots, with the budding on vegetatively propagated rootstocks of a first-grade commercial variety at a height of over 15 cm above the soil surface. One of the alternative ways of obtaining branched annual plants is wood grafting in spring and cultivation both in the field and in protected ground with a root-ball system. According to most researchers, the greatest effectiveness of the combined use of various techniques can be achieved only with strict adherence to conventional technologies for each method of cultivation.

KONTRIBUSI USAHATANI GULA AREN TERHADAP PENDAPATAN KELUARGA DI DESA TANGKENO KECAMATAN KABAENA TENGAH (Contribution of palm sugar farming to family income in Tangkeno village, central Kabaena subdistrict)

Sugar palm is a type of palm plant that is almost spread throughout Indonesia. All parts of this plant can be used, starting from the sap which can be processed into sugar, and the stems can be processed into palm flour, the unripe fruit can be processed into palm fruit, and the fibers can be processed into crafts. The aim of this research is to find out how much palm sugar farming contributes to family income in Tangkeno Village, Central Kabaena District. Meanwhile, the benefit of this research is to provide information regarding how much palm sugar processing contributes to family income in Tangkeno Village, Central Kabaena District. The method used in this research is the interview method. The types of data used to conduct research are primary data and secondary data. The results of research from 38 respondents show that the average contribution to palm sugar farming income in Tangkeno Village, Central Kabaena District for one year is IDR 22.934.210 with an average amount of palm sugar production of 4.323 kilograms, so the annual contribution of palm sugar farming is by 55%. The amount of income from palm sugar farming is quite large and is able to help the family to meet their needs. Keywords: Contribution, Income, Palm Sugar Farming Business, Tangkeno Village, Central Kabaena District

Response of Tomato Plants, Ailsa Craig and Carotenoid Mutant tangerine, to Simultaneous Treatment by Low Light and Low Temperature.

Tomato (Solanum lycopersicum L.) plants, wild type Ailsa Craig, and carotenoid mutant tangerine that accumulates prolycopene instead of all-trans-lycopene were exposed to a combined treatment by low light and low temperature for 5 days. The ability of plants to recover from the stress after development for 3 days at control conditions was followed as well. The suffered oxidative stress was evaluated by the extent of pigment content, lipid peroxidation, membrane stability, and H2O2 generation. The level of MDA content under combined treatment in tangerine implies that the mutant demonstrates lower sensitivity to stress in comparison with Ailsa Craig. The oxidative protective strategy of plants was estimated by following the antioxidant and antiradical activity of phenolic metabolites, including anthocyanins, as well as the activities of antioxidant enzymes superoxide dismutase (SOD), ascorbate peroxidase (APX) and catalase (CAT). Presented results revealed that the oxidative stress was much stronger expressed after exposure of both types of plants to low light combined with low temperature compared to that after treatment with only low light. The most significant antioxidant protection was provided by phenolic substances, including anthocyanins. The lower sensitivity of tangerine plants to low light can be attributed to the higher activity of the antioxidant enzyme CAT.

Litter decomposition in pure and mixed plantations on the Loess Plateau, China: Lack of home-field advantage

Litter decomposition plays a critical role in the nutrient cycling of terrestrial ecosystems. According to the home-field advantage (HFA) hypothesis, litter decomposes more rapidly in its home habitat (where it originates) compared to other habitats (referred to as away). Although this hypothesis has been widely tested in natural forests, it remains controversial and lacks support for plantations, especially in dry ecosystems where microbial activities are limited. To clarify it, the present study investigated litter mass loss, nutrient dynamics, and decomposer communities in a 549-day reciprocal transplant experiment of litter decomposition in Robinia pseudoacacia (RP) and Hippophae rhamnoides (HR) pure plantations and their mixed plantations (5:5 and 8:2 ratios) on the Loess Plateau, China. The rate of mass, carbon (C), nitrogen (N) and phosphorus (P) loss in litter differed depending on litter type of species component and plantation type they were placed in. HR litter lost mass and released C, N, and P fastest, irrespective of plantation type. The overall mass loss of the four litter types were the fastest under 5:5 mixed plantations. However, litter did not decompose more rapidly in its home soil compared to the away soil. The structural equation modeling revealed that initial litter quality played a significant role in regulating the loss of C and N across decomposition stages, while the climate variations and microbial chemistry also contributed to the process. The similarity in soil microbial communities across plantation types, along with the limited interaction between litter and microbes in arid environments, could help elucidate the lack of HFA, with other factors such as the litter type exerting a minor influence. In summary, this study does not substantiate the hypothesis of home-field advantage but supports its context-dependency. Thus, regional heterogeneity should be taken into account when predicting the carbon cycle of forest ecosystems in future studies.

Underwood cropping method in preventing soft rot disease of Amorphophallus konjac—from soil properties to rhizosphere microbial communities

Underwood cropping methods have been considered an effective way to prevent bacterial soft rot in Amorphophallus konjac，however, the relationship between rhizosphere soil properties, microbial communities and konjac soft rot under different planting types is not clear. In this study, we assessed the differences in soil nutrients and rhizosphere microorganisms of single-cropping, intercropping, and underwood-cropping konjac plants in an experimental field. The results show that the soil nutrient abundance in the rhizosphere soil and leaves of konjac underwood cropping were the highest. Moreover, the abundance and alpha diversity of root-associated bacteria such as Actinobacteria, Acidobacteria, and Nitrospirae were the most abundant in underwood cropping sites. The presence of these bacteria was significantly positively correlated with soil phosphatase, catalase, organic matter, available nitrogen, and available potassium. All these characteristics greatly improved the konjac resistance to soft rot pathogenic bacteria. This research may provide support for konjac soft rot prevention.

Method of first-approximation calculation of take-off weight of a light aircraft with a hybrid propulsion system

The article discusses a method for calculating, as a first approximation, the take-off weight of a light aircraft with a hybrid power plant based on piston and electric engines. A brief overview of organizations dealing with hybrid power plants is given. The influence of the degree of hybridization of the power plant on the take-off weight of the aircraft is shown. The degree of hybridization of a power plant is understood as a relative value characterizing the distribution of the total power of all engines installed on the aircraft between piston and electric engines. The main parameters necessary to determine the take-off weight of an aircraft to a first approximation are presented. The take-off weight of the aircraft is determined to a first approximation from the equation of its existence. Statistical data of aircraft with different types of power plants are provided on the basis of which calculations are carried out. As a first approximation, the relative mass of the hybrid power plant is determined by analyzing the statistical data of light aircraft with piston and electric engines, from which the corresponding graphs are constructed. After the calculations, conclusions were drawn about the optimal range of characteristics of a light aircraft with a hybrid power plant.

Association between total, animal, and plant protein intake and type 2 diabetes risk in adults: A systematic review and dose-response meta-analysis of prospective cohort studies

Background and AimsWhile clinical studies indicate that dietary protein may benefit glucose homeostasis in type 2 diabetes (T2D), the impact of dietary protein, including whether the protein is of animal or plant origin, on the risk of T2D is uncertain. We conducted a systematic review and meta-analysis to evaluate the associations of total, animal, and plant protein intakes with the risk of T2D. MethodsA systematic search was performed using multiple data sources, including PubMed/Medline, ISI Web of Science, Scopus, and Google Scholar, with the data cut-off in May 2023. Our selection criteria focused on prospective cohort studies that reported risk estimates for the association between protein intake and T2D risk. For data synthesis, we calculated summary relative risks and 95% confidence intervals for the highest versus lowest categories of protein intake using random-effects models. Furthermore, we conducted both linear and non-linear dose-response analyses to assess the dose-response associations between protein intake and T2D risk. ResultsSixteen prospective cohort studies, involving 615,125 participants and 52,342 T2D cases, were identified, of which eleven studies reported data on intake of both animal and plant protein. Intakes of total (pooled effect size: 1.14, 95% CI: 1.04-1.24) and animal (pooled effect size: 1.18, 95% CI: 1.09-1.27) protein were associated with an increased risk of T2D. These effects were dose-related – each 20-gram increase in total or animal protein intake increased the risk of T2D by ∼ 3% and ∼ 7%, respectively. In contrast, there was no association between intake of plant protein and T2D risk (pooled effect size: 0.98, 95% CI: 0.89-1.08), while replacing animal with plant protein intake (per each 20 gram) was associated with a reduced risk of T2D (pooled effect size: 0.80, 95% CI: 0.76-0.84). ConclusionsOur findings indicate that long-term consumption of animal, but not plant, protein is associated with a significant and dose-dependent increase in the risk of T2D, with the implication that replacement of animal with plant protein intake may lower the risk of T2D.