Convective heat transfer using nanofluids play an important role in thermal applications such as heat exchangers, automotive industries, and power generation. In this work, a numerical analysis is conducted to examine the heat transfer of nanofluid in three-dimensional differentially heated cavity. The finite volume method-based SIMPLEC algorithm is used to solve the system of the mass, momentum, and energy transfer governing equations. The left and the right vertical side walls of the cube are maintained at constant temperatures T C and T H , respectively. The remaining walls of the cube are insulated. Effective thermal conductivity and viscosity of the nanofluid are determined using Brinkman and Maxwell models, respectively. Studies are carried out for three types of nanoparticles and volume fractions of nanoparticles ( 0 – 5 % ). The effects of two binary liquid mixtures as a base fluid (propylene glycol-water and ethylene glycol-water) are also examined. Results show an enhancement of 13 % for Al2O3-EG in comparison to pure ethylene glycol in the case of Ra = 10 3 . In addition, heat transfer enhancement was increased with the rise of nanoparticle volume fractions.