SUMMARYSpores of PA 3679 were derived from vegetative cells made resistant to an initial 4.0 ppm tylosin. Rate of germination of PA 3679 spores, either from the original sensitive stock or from a resistant stock, is not affected by tylosin. Vegetative cells of PA 3679 arising from germination and subsequent outgrowth of the resistant cells are killed by 0.8–0.9 ppm of residual tylosin. This is to he compared with 0.05–0.25 ppm initial tylosin required to kill sensitive cells. The residual level of tylosin in sterile spore‐recovery media is dependent on the initial tylosin concentration and the medium.