Two-level Factorial Design Research Articles

Assessing the Effect of Specimen Preparation Methods on DSR Test Results of Bitumen Using Factorial Design Analysis.

A two-level three-factor factorial design experiment was conducted to study the influences of three critical specimen preparation parameters on the measurement results of bitumen by a dynamic shear rheometer (DSR). The investigated factors were (1) the pre-heating temperature (HT) for manufacturing the specimen, (2) the bonding temperature (BT) onto the rheometer, and (3) the trimming (Trim) operation for preparing the specimen after bonding. The analysed data were the measured shear modulus |G*|, phase angle δ, and the characteristic temperatures of bitumen's specific stiffness TX with corresponding phase angle δTX according to the European standard EN 14770:2023. Five types of bitumen were tested, including three penetration grades and two modified bitumen specimens (with polymer and wax additives). In addition, a repeatability evaluation of the test results was conducted. We found that the trimming operation for preparing the specimen has a noticeable impact when using smaller plates (PP08) for the DSR measurement. At higher test temperatures when using larger plates (PP25), the trimming operation does not significantly impact the measured parameters, in contrast to the HT and BT. Except for bitumen type 70/100, modified binders are more susceptible to variation in the analysed parameters than unmodified ones. The three-way interaction Trim:BT:HT tends to cause relatively little variation in measured data. Interactions between two factors Trim:BT, Trim:HT, and BT:HT contribute more to the fluctuation in δ value than in TX and |G*|. The variation employed in this study affects the test repeatability of wax-modified bitumen significantly; however, for unmodified binders the repeatability of TX and δTX are within 0.4-2.1 °C and 0.3-3.1°, respectively.

The Summary of Effect Aliasing Structure for Two-Level Supersaturated and Factorial Designs

The Summary of Effect Aliasing Structure for Two-Level Supersaturated and Factorial Designs

Rubber seed shell as low-cost medium to produce lactic acid using Lactobacillus plantarum LB2 and fabrication of a polylactic acid-chitosan composite for fish fillet packing

Rubber seed shell (Hevea brasiliensis) was used as a low-cost substrate to produce lactic acid via Lactobacillus plantarum LB2. The medium components were initially screened by two-level full factorial design. Three variables (pH, moisture, and polyoxyethylene sorbitan monooleate (PSM)) were used in the central composite design and response surface methodology. The amount of PSM was found to be a significant variable in lactic acid production. Lactic acid was purified and used for the chemical fabrication of a polylactic acid-chitosan composite film. Compared with the polylactic acid, the composite film improved the tensile strength, elongation strength, and tearing strength. The film prepared with 1% chitosan-polylactic acid exhibited the maximum antibacterial activity against Bacillus cereus (21 ± 1 mm) and the lowest activity against Escherichia coli (10 ± 1 mm). The polylactic acid-chitosan film prepared with 1% chitosan was used as a packing material to store the fish fillets and presented reduced mesophilic (4.3 ± 0.1 Log CFU/g) and psychrotropic (3.2 ± 0.2 Log CFU/g) bacterial populations compared with those of the control (4.9 ± 0.2 Log CFU/g and 3.7 Log CFU/g). Rubber seed shells can be used as an alternative culture medium for lactic acid production, to reduce the production cost of polylactic acid.

Efficient integration of analytical quality-by-design and tri-hued HPLC-DAD approach for synchronized assessment of gemigliptin and rosuvastatin in their fixed-dose pills: Application to stability study

Life-threatening atherosclerotic cardiovascular disorder is a well-recognized risk among diabetics, which is clinically ascribed to their unbalanced plasma lipoproteins. Gemigliptin-Rosuvastatin is a recently introduced polytherapy prescribed for the management of diabetic dyslipidemia. Currently, the main focus of several analysts is to design more ecologically friendly sustainable approaches for pharmaceutical analysis. In this context, an innovative tri-hued (green, blue, and white) stability-indicating HPLC-DAD approach was developed to concurrently quantify Gemigliptin and Rosuvastatin polypills. Consequently, a strategic integration between analytical quality-by-design and green/white analytical chemistry principles was specifically designed to optimize efficiency and practicality while minimizing ecological burden. Two experimental designs, namely two-level fractional factorial and Box-Behnken designs, were employed for screening and optimization procedures, respectively. Optimal separation was attained utilizing an Agilent HC-C18 column in conjunction with a mobile phase consisting of acetonitrile and 10 mM sodium dihydrogen phosphate buffer, pH 3 (60:40 v/v), eluted in an isocratic manner at a 1 mL/min flow rate. Both analytes exhibited good linearities within the concentration range of 0.5–100 μg/mL. Furthermore, stability studies were conducted on both drugs in response to variable stress degradation scenarios. Fortunately, the adopted approach was well-suited to resolve the studied drugs from their respective degradants, thereby verifying the method's stability-indicating power. Tri-hued evaluation for the implemented approach was performed via up-to-date metrics, specifically, the Analytical Eco-scale, AGREE, Blue Applicability Grade Index, and RGB 12. Ultimately, our adopted stability-indicating HPLC approach exhibited great sensitivity, specificity, applicability, and rapidity as well as being ecologically sound. It is worth noting that this is the first stability-indicating HPLC approach for simultaneous assessment of the studied drugs.

Natural deep eutectic based dispersive liquid-liquid microextraction and solidified floating organic drop for cadmium determination by electrothermal atomic absorption spectrometry with multivariate optimization (DLLME-SFOD-NADES-ETAAS)

A novel method was developed for cadmium determination based on dispersive liquid-liquid microextraction and solidified floating organic drop by multivariate optimization with electrothermal atomic absorption spectrometric detection using a natural deep eutectic solvent (NADES) of (L)-menthol and formic acid. Important factors, including pH, final concentration of PAN, mole ratio of formic acid, volume of NADES, type of dispersive solvent, and vortex time, were assessed using two-level fractional factorial design (FFD), and circumscribed central composite design (CCC). The analytical performance parameters, encompassing linearity range, limit of detection (LOD), limit of quantification (LOQ), and precision, were evaluated. The linearity range ranged from 0.72 to 2.50 ng/L, with corresponding LOD and LOQ values of 0.22 and 0.72 ng/L, respectively. Precision was evaluated by conducting three replicates of the three concentration levels, resulting in %RSD values of 11.8 %, 5.1 %, and 3.9 %, respectively. An enrichment factor (EF) of 112 was achieved. The optimized method was applied for cadmium determination in various matrices, including drinking water, environmental water, seafoods, yielding %recovery in the ranges of 78.05–102.41 %. Finally, comparison of t-values between the developed method and CRMs demonstrated satisfactory results at a 95 % confidence level.

Two-level factorial designs in block size four

Two-level factorial designs in block size four

A novel QbD HPLC approach for the concurrent analysis of amoxicillin, amprolium, and ethopabate in food samples and dosage forms

A simple, rapid, and sensitive reversed-phase HPLC method coupled with photodiode array detection (PDA) was developed for the simultaneous estimation of three co-administered antibacterial drugs, amprolium, ethopabate, and amoxicillin for the first time. An analytical quality-by-design (QbD) approach was adopted to optimize the developed approach. A two-level full factorial design (25 FFD) was implemented to optimize how variable factors affected chromatographic responses. On a Hypersil BDS C18 column, the chromatographic separation was carried out using isocratic elution at 40.1 °C. The mobile phase consisted of methanol: potassium phosphate buffer containing 5.76 mM heptane sulphonate (9.05 mM; pH 3.32, 44.8:55.2 % v/v) pumped at a flow rate of 0.8 mL/min and an injection volume of 20.0 μL. In less than 7 min, the suggested method was able to separate the ternary mixture with a challenging ratio of 1: 15.6: 1 for amoxicillin, amprolium, and ethopabate, respectively. The calibration curves showed excellent linearity over the concentration ranges of 0.50–30.0, 0.30–30.0, and 0.05–20.0 μg/mL, respectively. Recovery percentages ranged from 90.30 % to 111.13 % with %RSD less than 2 % were obtained upon spiking to chicken tissues, liver, and egg samples, indicating the suitability of the proposed method for determining of the studied drugs in complicated food matrices. Furthermore, the proposed method was successfully applied to determine the studied drugs in their commercial formulations. Full validation was carried out according to ICHQ2 (R1) guidelines. Finally, the method's greenness and ecofriendliness were evaluated using various metrics tools, including the Green Analytical Procedure Index (GAPI) and Analytical GREEnness (AGREE).

Medium Formulation and Optimisation of Fermentation Condition Enhancing γ-aminobutyric Acid (GABA) Biosynthesis by Lactiplantibacillus plantarum B7

Extensive studies on γ-aminobutyric acid (GABA) over decades highlight its significant physiological and pharmacological effects on humans. GABA produced using microbe is favoured compared to enzymatic and chemical methods due to operational ease and reduced harmful pollutant formation. This study focused on increasing γ-aminobutyric acid (GABA) biosynthesis from Lactiplantibacillus plantarum B7, employing a multi-step optimisation strategy. An unoptimised cultivation approach yielded a maximum GABA of 11.68 ± 0.04 g/L and viable cell count of 10.47 ± 0.01 log CFU/mL at 48 h. A nutrient-rich medium was developed through single-parameter optimisation, comprising 1%, 2.5% and 0.0002% of glucose, yeast extract and each trace element (CaCO3, KI, and Tween 80) respectively. Temperature, pH, incubation period, initial concentration of monosodium glutamate (MSG) and pyridoxal-5’-phosphate (PLP) demonstrated significant contributions towards GABA production and cell growth as determined using a two-level factorial design. Steepest ascent identified optimal conditions (36°C, pH 5.5, 370 mM MSG, and 0.7 mM PLP), resulting in 30.50 g/L GABA and 11.51 log CFU/mL at 60 h. Further refinement via a central composite experiment yielded optimal conditions (temperature-35.6°C, pH-5.66, initial MSG concentration-335.61 mM, PLP concentration-0.723 mM) with improved GABA production (32.18 g/L) and cell growth (11.52 log CFU/mL) over 63.66 h. Therefore, this approach utilising lactic acid bacteria capable of GABA synthesis holds promise for mass-produced, enhanced-functional foods.

A practical approach to demonstrate the circular economy in remediation of textile dyes using nutraceutical industrial spent.

We used Nutraceutical Industrial Coriander Seed Spent (NICSS), a readily available, cheap, eco-friendly, and ready-to-use material, as an innovative adsorbent for the bioremediation of a bisazo Acid Red 119 (AR 119) dye, which is likely a mutagen from textile industrial effluents (TIE). A laboratory-scale experiment was tailored to demonstrate the framework of the circular economy (CE) in the remediation of textile dyes using Nutraceutical Industrial Spent to align with the principles of sustainability and valorization. An experimental q e value of 97.00 mg g-1 was obtained. For the practicality and effectiveness of the method, a two-level fractional factorial experimental design (FFED) was employed to determine variables that influence the adsorption capacity of NICSS. At optimal settings (pH of 1.4, adsorbent dosage of 6.000 g L-1, adsorbent particle size of 96 μm, initial dye concentration of 599 mg L-1, adsorption duration of 173 min, orbital shaking speed of 165 rpm, and temperature of 35 °C), the maximum adsorption efficiency achieved through statistical optimization was 614 mg g-1. Six factors influencing the adsorption process were examined experimentally and were considered important for commercialization. Three orders of magnitude were applied to the identified variables in scaling experiments. Adsorption-equilibrium data were analyzed using nine isotherm models. The best fit was discovered to be the Vieth-Sladek adsorption isotherm model. The suitable mechanism for the overall rate of the adsorption process was a pseudo-second-order reaction: mass-transfer mechanistic studies were predicted to predominate over the diffusion process. NICSS was characterized using SEM and FTIR spectroscopy. Utilizing plastic trash, the dye-adsorbed NICSS that was recovered as "sludge" was utilized as a reinforcing material to create composites. Dye-adsorbed NICSS thermoplastic and thermoset composites were studied and compared with NICSS composites in terms of their physicomechanical and chemical properties.

Novel Microwave Assisted-Hydrogen Peroxide Digestion of Coal and Fuel Oils Using Methionine as a Capping Reagent for the Determination of Mercury by Inductively Coupled Plasma-Optical Emission Spectrometry (ICP-OES)

A novel analytical method was developed for quantifying total mercury in fuel oils using methionine as a capping reagent. Methionine was added to fuel and standard solutions as a preserving agent before microwave-assisted hydrogen peroxide digestion (MW-AHPD). To the best of our knowledge, it is the first-time methionine was used as a capping reagent for the total determination of mercury in fuel oils using ICP-OES with a pneumatic nebulizer. Multivariate optimization tools (two-level half factorial and central composite design) were used to investigate the most influential parameters of the MW-AHPD method. The best conditions were 0.1 g sample mass, 5 mol L−1 [H2O2], 5. 95 mol L−1 [methionine], 36 min and 200 °C with accepted trueness (93–107%) and precision (1.94%). The developed MW-AHPD procedure provided a coefficient of determination (R2) of 0.9989, limit of detection (LOD) of 0.25 µg L−1 and limit of quantification (LOQ) of 0.8 µg L−1. The newly developed MW-AHPD procedure was validated using hydride generation-ICP-OES and there was no statistical difference at 95% confidence level between the newly developed method and the standard protocol. Therefore, the newly developed MW-AHPD procedure was applied with coal, crude oil, gasoline, diesel oil, and kerosine samples and the concentration levels were from 0.876 ± 0.023 to 0.938 ± 0.082 µgg−1, 0.383 ± 0.043 to 0.506 ± 105 µgg−1, 0.306 ± 0.010 to 0.390 ± 0035 µgg−1, 0.360 ± 0.003 to 0.431 ± 0.001 µgg−1 and < DL, respectively.

Maximizing the Use of Ivermectin Transethosomal Cream in the Treatment of Scabies.

In an effort to tackle the skin reactions frequently observed with topical application of ivermectin (IVM), a study was conducted to develop and optimize transethosomes (TESMs) loaded with IVM for scabies treatment. A three-factor, two-level (23) full factorial design was employed. Soyabean phosphatidylcholine concentration (A), ethanol concentration (B) and Span 60 amount (C) were studied as independent factors, while entrapment efficiency (EE), particle size (PS), polydispersity index (PDI), zeta potential (ZP) and drug release after 6 h (Q6h) were characterized. The skin sensitivity of the optimized formulation was evaluated by skin irritation test and histopathological examination. The EE% ranged from 88.55 ± 0.576% to 94.13 ± 0.305%, PS was from 318.033 ± 45.61 nm to 561.400 ± 45.17 nm, PDI was from 0.328 ± 0.139 to 0.671 ± 0.103, ZP was from -54.13 ± 1.09 mV to -60.50 ± 2.34 mV and Q6h was from 66.20 ± 0.30% to 93.46 ± 0.86%. The IVM-loaded transethosomal cream showed lower skin irritation and a more intact epidermal layer with intact keratinocyte, compared to the marketed cream which showed severe destruction of the keratin layer. Therefore, patient compliance can be improved by encapsulating IVM within TESMs to minimize its skin reactions.

Optimizing methylene blue adsorption conditions on hydrothermally synthesized NaX zeolite through a full two-level factorial design.

Besides being hazardous to humans and aquatic organisms, dyes present in water reservoirs limit sunlight's availability to aquatic plants and animals, making significant impact on their growth and development. Herein, the adsorptive removal of methylene blue (MB) dye from aqueous solution using type X (NaX) zeolite by full experimental design 2 n was studied. The physical and chemical properties of NaX zeolite were identified using various characterization techniques such as X-ray diffraction (XRD), scanning electron microscopy with energy dispersive X-ray (SEM-EDS), Fourier transform infra-red (FT-IR), and Brunauer-Emmett-Teller (BET) surface area analyses. Results confirmed that NaX zeolite had a cubic shaped crystalline structure with 2-4 μm size and high (375 m2 g-1) specific surface area, having 90% optimal adsorption efficiency. Langmuir and Elovich isotherm models were best fitted to adsorption experimental data and a pseudo-second-order kinetic model describes well the adsorption kinetic data. Akaike information criteria (AIC) was used to assess the best fitted models on the experimental data. A thermodynamic study reveals that the MB adsorption onto NaX was exothermic, spontaneous, and feasible.

Design and modeling of order of addition experiment with component effects

An order of addition experiment is an experiment to study how the order of addition of components affect the results, with the objective of predicting and determining the optimal order of addition of components. Order of addition experiment are also commonly used in the drug combination therapy, where experimenting with all drugs combinations is unaffordable. To solve this problem, we constructed a new design table, two-level component factorial design table (TLCF), which combine the component orthogonal array design table and the two-level partial factorial design table by matrix product. TLCF can explore the order and dosage effect of components on the results and can greatly reduce the number of experiments. We also prove that the relative D-efficiency of the TLCF can reach 100% and solve an explicit expression for the D-efficiency of the full design. In the simulation experiment, we compare the D-efficiency of the TLCF with the random design table to prove the superiority of TLCF. Finally, we give a treatment plan for the combination of three drugs for glioblastoma based on the TLCF, which provides a new perspective for the precision treatment of patients.

An investigation into the aging mechanism of disposable face masks and the interaction between different influencing factors

In the natural environment, a symphony of environmental factors including sunlight exposure, current fluctuations, sodium chloride concentrations, and sediment dynamics intertwine, potentially magnifying the impacts on the aging process of disposable face masks (DFMs), thus escalating environmental risks. Employing Regular Two-Level Factorial Design, the study scrutinized interactive impacts of ultraviolet radiation, sand abrasion, acetic acid exposure, sodium chloride levels, and mechanical agitation on mask aging. Aging mechanisms and environmental risks linked with DFMs were elucidated through two-dimensional correlation analyses and risk index method. Following a simulated aging duration of three months, a single mask exhibited the propensity to release a substantial quantity of microplastics, ranging from 38,800 ± 360 to 938,400 ± 529 particles, and heavy metals, with concentrations from 0.06 ± 0.02 μg/g (Pb) to 29.01 ± 1.83 μg/g (Zn). Besides, specific contaminants such as zinc ions (24.24 μg/g), chromium (VI) (4.20 μg/g), thallium (I) (0.92 μg/g), tetracycline (0.51 μg/g), and acenaphthene (1.73 μg/g) can be adsorbed significantly by aged masks. The study elucidates pivotal role of interactions between ultraviolet radiation and acetic acid exposure in exacerbating the environmental risks associated with masks, while emphasizing the pronounced influence of many other interactions. The research provides a comprehensive understanding of the intricate aging processes and ensuing environmental risks posed by DFMs, offering valuable insights essential for developing sustainable management strategies in aquatic ecosystems.

Fluconazole-Niosome-Laden Contact Lens: A Promising Therapeutic Approach for Prolonged Ocular Delivery and Enhanced Antifungal Activity

BackgroundTraditional routes of administration of fluconazole such as eye drops have a low therapeutic efficacy due to insufficient bioavailability.PurposeHerein, a fluconazole noisome-laden contact lens was prepared to control and prolong the drug release and improve its bioavailability.MethodsTwo methods have been used to prepare fluconazole niosomes: solvent injection method and thin film hydration method utilizing span 60 and cholesterol mixture. Subsequently, formulations were optimized using three factors and a two-level factorial design and were subjected to in-vitro characterization for the size of niosomes, zeta potential, entrapment efficiency percent, and cytotoxicity study. The optimized fluconazole niosomes were further entrapped in contact lenses by the soaking method and were evaluated according to in-vitro release profile, and antimicrobial activity.ResultsThe results revealed that the investigated fluconazole niosomes are of nano-size ranging from 228.2 to 769.2 nm with zeta-potential values between − 18.1 and − 60.2 mV. The entrapment efficiency percentage ranged from 51.3 to 75%. Fluconazole was released from fluconazole noisome-laden contact lens and showed a prolonged release up to 48–72 h with a cumulative release of 79.62%. Statistical analysis showed that fluconazole-noisome-laden contact lenses have a significant impressive fungal adhesion reduction as compared to fluconazole-laden contact lenses.ConclusionFluconazole noisome-laden contact lenses are a promising therapeutic way for effective and prolonged treatment of ocular fungal infection.

Aspergillus niger as an efficient biological agent for separator sludge remediation: two-level factorial design for optimal fermentation.

The booming palm oil industry is in line with the growing population worldwide and surge in demand. This leads to a massive generation of palm oil mill effluent (POME). POME is composed of sterilizer condensate (SC), separator sludge (SS), and hydro-cyclone wastewater (HCW). Comparatively, SS exhibits the highest organic content, resulting in various environmental impacts. However, past studies mainly focused on treating the final effluent. Therefore, this pioneering research investigated the optimization of pollutant removal in SS via different aspects of bioremediation, including experimental conditions, treatment efficiencies, mechanisms, and degradation pathways. A two-level factorial design was employed to optimize the removal of chemical oxygen demand (COD) and turbidity using Aspergillus niger. Bioremediation of SS was performed through submerged fermentation (SmF) under several independent variables, including temperature (20-40°C), agitation speed (100-200 RPM), fermentation duration (72-240 h), and initial sample concentration (20-100%). The characteristics of the treated SS were then compared to that of raw sludge. Optimal COD and turbidity removal were achieved at 37°C 100 RPM, 156 h, and 100% sludge. The analysis of variance (ANOVA) revealed a significant effect of selective individual and interacting variables (p<0.05). The highest COD and turbidity removal were 97.43% and 95.11%, respectively, with less than 5% error from the predicted values. Remarkably, the selected optimized conditions also reduced other polluting attributes, namely, biological oxygen demand (BOD), oil and grease (OG), color, and carbon content. In short, this study demonstrated the effectiveness of A.niger in treating SS through the application of a two-level factorial design.

Effects of Layer Thickness and Compaction Thickness on Green Part Density in Binder Jetting Additive Manufacturing of Silicon Carbide: Designed Experiments

This paper reports on an experimental investigation that used a full factorial design to study the main effects and the interaction effect of layer thickness and compaction thickness on the green part density in the binder jetting additive manufacturing of silicon carbide. A two-variable, two-level full factorial design was employed. The results show that the green part density was higher at the low level of layer thickness and at the high level of compaction thickness. These results can be useful in selecting the values of printing variables, enabling the fabrication of green parts with a desirable density that is crucial for advanced ceramic applications.

Effect of Protein-Starch Interaction on Rheological, Textural, and Sensory Properties of &lt;i&gt;keropok lekor&lt;/i&gt;

This article considers the effect of protein–starch interaction on the gelling, textural, andsensory properties of keropok lekor used as a fish protein–starch model. A two-level factorial design was employed to analyze the quality and acceptability of different formulations of keropok lekor crackers depending on the ratios of minced fish (MF, 20–50 g (w/w)), sago starch (SS, 10–40 g (w/w)), and water (W, 10–35 g (w/w)). The parameters measured were the onset (T0) and peak (Tp) temperatures of gelatinization, storage modulus (G′), and loss modulus during gelatinization (G″). The samples were rated by a group of 30 panelists during texture profile analysis and sensory evaluation. The most preferred samples had the MF : SS : W ratio of 20 : 10 : 10 and were characterized by the lowest onset and peak temperatures of gelatinization. Therefore, this formulation was singled out as optimal for keropok lekor.

Green micellar factorial design optimized first derivative synchronous spectrofluorimetric method for tripelennamine and diphenhydramine determination in pharmaceutical gel.

A green micellar synchronous spectrofluorimetric method was developed and validated for simultaneous determination of tripelennamine hydrochloride and diphenhydramine in bulk and combined pharmaceutical formulation. Synchronous fluorescence of tripelennamine hydrochloride and diphenhydramine was determined using Δλ = 60 nm. The first derivative of synchronous fluorescence was computed to resolve overlap in the synchronous fluorescence spectra. Tripelennamine hydrochloride was quantified at 375 nm, whereas diphenhydramine was quantified at 293 nm; each is the zero-crossing point of the other. As diphenhydramine exhibited weak native fluorescence, micelle enhancement upon incorporation of sodium dodecyl sulfate was considered. Two-level full factorial design was carried out to optimize experimental parameters. Optimum conditions involved using SDS (2% w/v) along with Teorell and Stenhagen buffer (pH9). The method was found to be linear over the range 0.2-4.5 and 0.2-5μg/mL for tripelennamine and diphenhydramine, respectively, with limits of detection 0.211 and 0.159 μg/mL. The method was successfully applied for simultaneous determination of tripelennamine hydrochloride and diphenhydramine in laboratory-prepared gel containing all possible excipients with mean percent recoveries ±SD 100.59 ±0.79 and 98.99 ±0.98 for tripelennamine hydrochloride and diphenhydramine, respectively. The proposed method was proved to be eco-friendly using different greenness assessment tools.

Utilization of Ketapang Seed Shells in Reducing Pb(NO&lt;sub&gt;3&lt;/sub&gt;)&lt;sub&gt;2&lt;/sub&gt; Metal Levels by Adsorption Process

Industrial wastes such as lead metal can cause serious problems because of their toxic nature and can pollute the environment. To reduce levels of lead metal, you can use a natural adsorbent from activated charcoal on the outer shell of ketapang seeds. The ketapang tree is a type of plant that is often found in the surrounding environment because it is used as a shade for the many falling ketapang seeds making waste in the environment to reduce environmental pollution from the falling ketapang can be used as an adsorbent because it contains cellulose and carbon elements. One alternative to reduce lead levels using adsorbents is through the adsorption process which is the process of adsorption of a solid or liquid that involves physical, chemical and electrostatic interactions between the adsorbate and the adsorbent on the surface of the adsorbent. The adsorption process using activated charcoal adsorbent is one of the most frequently used non-polar adsorbents because it has better adsorption power and surface area than other types of adsorbents. This study aims to determine the influencing variables and the optimum condition of the natural adsorbent from the activated charcoal of the outer shell of ketapang seeds on decreasing levels of metal Pb (NO3)2. The research method used is an experimental method with a two-level factorial design. The results showed that the adsorption temperature was the most influential variable in this study with an optimum yield presentation of 66.77%. The activated charcoal produced has a water content of 8%, ash content of 5.2% and an absorption capacity of methylene blue of 698 mg/g.