This paper discusses a new model and experimental characterization for thermal responses of straight twisted polymer fiber (TPF) actuators in blocked torsion. The proposed model is derived from combining the fiber stiffness matrix with Shafer’s model of free torsion. The derived model predicts that the blocking torque increases as the initial twist increases. Although it is not widely recognized, the tension of a straight TPF decreases as the initial torsion increases, and this paper explains this fact. The proposed model predicts the decrease in tension by using Shafer’s kinematic model that considers the effect of radial thermal expansion. Experimental characterization and model verification are performed using nylon 6 (polyamide 6) fiber samples with ten different initial twists, ranging from weak to strong. The results indicate that as the initial twist increases, the torque increases and the tension decreases. The tension fluctuates with respect to time. The experimental results show that the shear modulus increases as the initial twist increases. The predictions of the proposed model are reasonably consistent with the experimental thermal responses of straight TPFs in blocked torsion when the constants obtained by experimental characterization are used.