Continuously twisted polyacrylonitrile/viscose nanofiber core-spun yarns were fabricated through novel self-designed multi-nozzle air jet electrospinning set-up. The effect of voltage, solution flow rate, air flow rate and funnel rotating speed on coating rate of core-spun yarn, nanofiber diameter, twist level and mechanical property were discussed. The results showed that polyacrylonitrile/viscose nanofiber core-spun yarns with perfect nanofiber orientation and uniform twist distribution could be obtained at voltage of 32 KV, solution flow rate of 32 ml/min and air flow rate of 1000 ml/min, and the spinning speed could reach to 235.5 cm/min. The diameters of outer coated nanofiber distributed from 100 nm to 300 nm, and nanofiber coating rate could reach to 70.4 %. In addition, the strength and elongation at break increased from 30.82 MPa to 69.65 MPa and from 28.34 % to 43.29 % at the twist angle of 46.6 °, respectively.