The effects of extrusion cooking on the residual activities of six enzymes in wheat, rye and oats and several milling products were investigated. In general,enzyme inactivation increased as extrusion temperature increased, α-Amylase activity in oats, however, was higher in some extrusion products than in the original grain. The thermostable enzymes (lipolytic acyl hydrolase, α-amylase, and peroxidase) differed consistently from the thermostable enzymes (α-benzoyl-L-arginine-p-nitroanilide-hydrolase, catalase and lipoxygenase). Wheat bran, broken and unbroken oat kernels were extruded at two moisture levels (18 and 30%). Whereas lipolytic aryl hydrolase activity was lower in material extruded at 30% moisture, residual α-amylase activities were higher at this moisture level. Peroxidase activity in oats, unlike that in wheat bran, was inactivated to a greater extent at the 30% than at the 18 % moisture level.
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