Nutrient Turnover Research Articles

Groundwater flow reversal between small water bodies and their adjoining aquifers: A numerical experiment

AbstractThe countless kettle holes in the Late Pleistocene landscapes of Northern Europe are hotspots for biodiversity and biogeochemical processes. As a rule, they are hydraulically connected to the shallow groundwater system. The rapid, intensive turnover of carbon, nutrients and pollutants in the kettle holes therefore has a major impact on the quality of the shallow groundwater downstream. As a result of high‐evapotranspiration rates from their riparian vegetation or strong storm events, the process of downstream groundwater flow may stagnate and reverse back towards the kettle hole, making interactions between the groundwater and kettle hole more complex. Furthermore, the highly heterogeneous soil landscape in the catchment contributes to this complexity. Therefore, the present study aims to enhance our understanding of this complicated interaction. To this end, 24 model variants were integrated into HydroGeoSphere, capturing a wide range of uncertainties in quantifying the extent and timing of groundwater flow reversal between a kettle hole and the adjacent aquifer. The findings revealed that the groundwater flow reversal lasted between 1 month and 19 years at most and occurred in a distance of more than 140 m downstream of the kettle hole. Our results demonstrated that the groundwater flow reversal arises especially often in areas where the shallow aquifer possesses low‐hydraulic conductivity. There may also be a recurrent circulating flow between the groundwater and kettle hole, resulting in solute turnover within the kettle hole. This holds particularly true in dry periods with medium to low‐water levels within the kettle hole and a negative water balance. However, shallow groundwater flow reversals are not necessarily a consequence of seasonal effects. In this respect, the properties of the local shallow aquifer by far outweigh the effect of the kettle hole location in the regional flow regime.

Long-Term Nitrogen Addition Exerts Minor Effects on Microbial Community but Alters Sensitive Microbial Species in a Subtropical Natural Forest

Increasing nitrogen (N) deposition profoundly affects nutrient cycling in soil, thereby influencing forest ecosystem productivity and function. Soil microorganisms are integral in driving nutrient turnover; the changes in microbial communities in response to N deposition and the associated soil nutrient availability, especially of limited nutrients, are far from clear. To explore the changes in soil bacterial and fungal communities and their key environmental drivers under N deposition, we conducted a multilevel field N addition experiment in a Castanopsis carlesii natural forest. Soil properties and bacterial and fungal communities were investigated. There were no significant changes in alpha diversities (presented as Chao1 and Shannon’s indexes) and beta diversities of bacteria and fungi among the three treatments. Consistently, the relative abundances of dominant bacterial phyla (i.e., Proteobacteria, Acidobacteria, Actinobacteria, Chloroflexi, and Planctomycetes) and fungal phyla (i.e., Basidiomycota, Ascomycota, and Rozellomycota) did not change following N addition. These results suggest that N deposition did not alter microbial community diversity and structure. In addition, the results of the Mantel test showed that soil pH, NO3−-N, dissolved organic N (DON), and total phosphorus (TP) predominantly influenced the community diversity and structure in bacteria, but not in fungi. Meanwhile, the relative abundance of some sensitive microbial genera, such as Bryobacter, Bradyrhizobium, Sorangium, and Archaeorhizomyces, were significantly decreased. These results indicate a decreased microbial ability for N fixation and P mobilization induced by N deposition. Moreover, there were significant relationships between Bryobacter, Bradyrhizobium, and Archaeorhizomyces and NO3−-N and available P (AP), suggesting that the responses of sensitive microbial groups to N deposition likely depend on the changes in available nutrients in soil, especially limited N or P. Collectively, 6 years of N addition had no significant influence on microbial communities, but some sensitive microbial groups were associated with N or P turnover. This finding emphasizes the critical roles of sensitive microbial species in meditating limited nutrient cycling in soil under climate change.

An improved non-point source pollution model for catchment-scale hydrological processes and phosphorus loads

Non-point source (NPS) pollutants may cause water quality deterioration and eutrophication, posing a significant threat to aquatic systems. Understanding and modelling surface and sub-surface hydrological processes and nutrient cycles are therefore essential for water resources management and pollution control. This paper presents a new hydrological-water quality model for application in humid and semi-humid catchments in China, considering both hydrological processes, and nutrient transport and transformation. The model divides the soil into three layers, and each layer is implemented with individual computational procedures for soil wetness and nutrient process. Nutrient transport is driven by hydrological processes and follows the same pathways as water flow in the model: surface runoff, infiltration, and outflow from individual soil layers. River channels are described separately with routines to account for the turnover of nutrients. Model parameters are selected according to literature/open-sourced data or estimated from soil texture or land use types. The model is applied to simulate the hydrological processes and phosphorus transport in the Tongyang River Basin in China and the model performance is confirmed by comparing the predicted discharge and total phosphorus concentrations with measured data at the catchment outlets of Tonghe River and Yanghe River over 3 years. Uncertainty analysis has been further carried out using the GLUE method to demonstrate sensitivity of the simulation results to the selection of model parameters. After model calibration, the predicted results are found to compare well with field observations in terms of flow discharge and total phosphorus concentration. From sensitivity analysis, it is found that the recession coefficient of channel system (CS) and the pollutant recession coefficient of surface runoff (pKS) are the most influential hydrological and water quality parameters that control the arrival time and duration of flood peak and nutrient concentration peak, respectively.

Reed biochar improved the soil functioning and bacterial interactions: A bagging experiment using the plantation forest soil (Fraxinus chinensis) in the Xiong'an new area, China

Common reed (Phragmatic australis) is a vigorous growing wetland plant that forms dense monotypic stands in the Baiyang Lake in the Xiong'an New Area (XNA) of Hebei Province in north China. As a result of untimely harvesting, it has recently developed into an endogenous invasive plant. We assessed the feasibility of reed biochar addition in soils from the ash tree (Fraxinus chinensis) plantation to evaluate the impact of biochar addition on the soil multi-functionality with a bag trial using a greenhouse incubation approach. The soil nutrient turnover was promoted by increasing the abundance of key functional genes of cbbL, nifH, nirK, and pqqC at the early stage. Bacterial taxa tended to be convergent communities irrespective of biochar addition. Path analysis showed that soil variables and bacterial network parameters were positively correlated with soil function in general, whereas alpha diversity has shown an almost opposite relationship. Biochar increased the number of keystone taxa at the treatment's middle stages and improved the network's stability. The whole primary succession of the bacterial communities remarkably homogenous dispersal, contributing to 80% of bacterial community assemblies, which indicates little competitive pressure. Collectively, these results point to a role for biochar addition in enhancing soil functions in ash tree plantations, which can be considered cleaner production to contribute to the greening efforts of the plantation forest sustainability.

Intracellular carbon storage by microorganisms is an overlooked pathway of biomass growth

The concept of biomass growth is central to microbial carbon (C) cycling and ecosystem nutrient turnover. Microbial biomass is usually assumed to grow by cellular replication, despite microorganisms’ capacity to increase biomass by synthesizing storage compounds. Resource investment in storage allows microbes to decouple their metabolic activity from immediate resource supply, supporting more diverse microbial responses to environmental changes. Here we show that microbial C storage in the form of triacylglycerides (TAGs) and polyhydroxybutyrate (PHB) contributes significantly to the formation of new biomass, i.e. growth, under contrasting conditions of C availability and complementary nutrient supply in soil. Together these compounds can comprise a C pool 0.19 ± 0.03 to 0.46 ± 0.08 times as large as extractable soil microbial biomass and reveal up to 279 ± 72% more biomass growth than observed by a DNA-based method alone. Even under C limitation, storage represented an additional 16–96% incorporation of added C into microbial biomass. These findings encourage greater recognition of storage synthesis as a key pathway of biomass growth and an underlying mechanism for resistance and resilience of microbial communities facing environmental change.

Straw return counteracts the negative effects of warming on microbial community and soil multifunctionality

Climate warming is one of the most serious threats to soil biodiversity and ecosystem stability. Straw return has been extensively recommended as an environmentally friendly management to increase soil health and agricultural productivity. However, little is known about their interactive effects on microbial communities and soil functioning. We investigated the effects of soil warming, straw return, and their interactions on soil microbial communities, functional genes, enzyme activities related to C, N and P cycling, and multifunctionality in a two-factorial experiment in a wheat-maize rotation system. Soil warming decreased fungal diversity (by 20%) and functional genes associated with organic matter decomposition, N fixation, nitrification, denitrification, and organic P mineralization compared with ambient temperature. Soil multifunctionality mediated by enzyme activities were reduced under warming because of lower soil moisture, nutrient availability, and root-derived labile organic matter inputs. The close relationships of microbial diversity and functional genes with soil multifunctionality highlighted the importance of soil biodiversity in maintaining agroecosystem functioning related to nutrient cycling. Under soil warming and ambient temperature, straw return resulted in higher bacterial diversity (by 3.6%) and microbial functional genes abundances of C, N and P cycling compared to straw removal, and consequently raised soil multifunctionality. This was primarily because straw buffered soil temperature amplitude and moisture variation, as well as the additional nutrient supply within the added straw. Overall, straw return created favorable habitats for microorganisms, and thereby mitigated the adverse effects of warming on microbial communities and soil functionality. Our study provided the possibility to increase soil biodiversity and further ecosystem services related to organic matter decomposition and nutrient turnover by straw addition, especially under climate warming.

Mesophilic and thermophilic viruses are associated with nutrient cycling during hyperthermophilic composting

While decomposition of organic matter by bacteria plays a major role in nutrient cycling in terrestrial ecosystems, the significance of viruses remains poorly understood. Here we combined metagenomics and metatranscriptomics with temporal sampling to study the significance of mesophilic and thermophilic bacteria and their viruses on nutrient cycling during industrial-scale hyperthermophilic composting (HTC). Our results show that virus-bacteria density dynamics and activity are tightly coupled, where viruses specific to mesophilic and thermophilic bacteria track their host densities, triggering microbial community succession via top-down control during HTC. Moreover, viruses specific to mesophilic bacteria encoded and expressed several auxiliary metabolic genes (AMGs) linked to carbon cycling, impacting nutrient turnover alongside bacteria. Nutrient turnover correlated positively with virus–host ratio, indicative of a positive relationship between ecosystem functioning, viral abundances, and viral activity. These effects were predominantly driven by DNA viruses as most detected RNA viruses were associated with eukaryotes and not associated with nutrient cycling during the thermophilic phase of composting. Our findings suggest that DNA viruses could drive nutrient cycling during HTC by recycling bacterial biomass through cell lysis and by expressing key AMGs. Viruses could hence potentially be used as indicators of microbial ecosystem functioning to optimize productivity of biotechnological and agricultural systems.

The Influence of Aerated Irrigation on the Evolution of Dissolved Organic Matter Based on Three-Dimensional Fluorescence Spectrum

In order to unravel the effect of aerated irrigation on soil dissolved organic matter (DOM) fluorescence characteristics, and humification degree, a randomized block experiment was conducted with three factors and a two-level design, i.e., two irrigation rates (0.6 and 1.0 times of crop evaporation pan coefficient, W1 and W2), two nitrogen application rates (225 and 300 kg hm−2, N1 and N2), and two aeration rates (15% and 0% in control treatment, A1 and A0). Fluorescence regional integration (FRI) and correlation analysis methods were used to investigate the evolution characteristics of the soil DOM fluorescence spectrum. Under aerated and conventional subsurface irrigation, soil DOM components were dominated by humic acid-like substances, fulvic acid-like substances, tryptophan-like proteins, and supplemented by tyrosine-like proteins and dissolved microbial metabolites. Soil aeration could promote the consumption of soil DOM components under low irrigation rates and accelerate the consumption of soil DOM components under high irrigation rates. The humification index of AI treatments varied from 8.47 to 9.94 during the maturity growth stage of pepper, averagely increased by 31.59% compared with the non-aeration treatment. To sum up, aerated irrigation can promote the depletion of small molecular proteins and accelerate nutrient turnover and the accumulation of big molecular proteins.

Forest microbiome and global change.

Forests influence climate and mitigate global change through the storage of carbon in soils. In turn, these complex ecosystems face important challenges, including increases in carbon dioxide, warming, drought and fire, pest outbreaks and nitrogen deposition. The response of forests to these changes is largely mediated by microorganisms, especially fungi and bacteria. The effects of global change differ among boreal, temperate and tropical forests. The future of forests depends mostly on the performance and balance of fungal symbiotic guilds, saprotrophic fungi and bacteria, and fungal plant pathogens. Drought severely weakens forest resilience, as it triggers adverse processes such as pathogen outbreaks and fires that impact the microbial and forest performance for carbon storage and nutrient turnover. Nitrogen deposition also substantially affects forest microbial processes, with a pronounced effect in the temperate zone. Considering plant-microorganism interactions would help predict the future of forests and identify management strategies to increase ecosystem stability and alleviate climate change effects. In this Review, we describe the impact of global change on the forest ecosystem and its microbiome across different climatic zones. We propose potential approaches to control the adverse effects of global change on forest stability, and present future research directions to understand the changes ahead.

Imprint of tree species mycorrhizal association on microbial‐mediated enzyme activity and stoichiometry

Abstract Understanding the effects of tree species and their mycorrhizal association on soil processes is critical for predicting the ecosystem consequences of species shifts owing to global change and forest management decisions. While it is well established that forests dominated by different mycorrhizal types can vary in how they cycle carbon (C), nitrogen (N) and phosphorus (P), the degree to which these patterns are driven by microbial‐mediated enzyme activity (EA) and ecoenzymatic stoichiometry (ES) remains elusive. Here, we synthesized the effects of mycorrhizal association on seven soil enzymes involved in microbial C, N and P acquisition and ES using data from 56 peer‐reviewed papers. We found that relative to soil in ectomycorrhizal (EcM) trees, soil in arbuscular mycorrhizal (AM) trees exhibited greater activity of some C acquisition enzymes (e.g. beta‐glucosidase; BG) and higher ecoenzymatic ratios of BG/NAG (N‐acetyl‐glucosaminidase) and BG/AP (acid phosphatase). These results supported that AM trees had rapid C and nutrient turnover rates, inorganic nutrient economics and high soil microbial C limitation. We also found evidence for an organic nutrient economy and greater soil microbial demand for nutrients in EcM trees compared to AM trees. In addition, the effect of mycorrhizal association on the activity of certain soil enzymes and enzymatic stoichiometry (i.e. BG and BG/NAG ratio) appeared to be associated with the differences in soil pH, phylogenetic group (i.e. conifers and broadleaves) and leaf habit (i.e. evergreen and deciduous) between AM and EcM trees. The results from the global meta‐analysis suggested that soil EA and ES appear to play critical roles in shaping the differences in the nutrient economy between AM and EcM tree species, but leaf morphology and soil conditions should be considered in evaluations of soil processes in forests of different mycorrhizal associations. Given that most of the studies in the database were from the temperate and subtropical regions, further research in other biomes is needed to elucidate the underlying mechanisms driving the mycorrhizal effect at the global scale. Read the free Plain Language Summary for this article on the Journal blog.

Investigating the response of soil nitrogen cycling to grass invasion

In heathlands, high mineral N input causes replacement of Calluna vulgaris, the dominant plant, by fast-growing grasses such as Molinia caerulea. The vegetation shift signifies altered litter quality from low- to high-quality litter due to differences in lignin content. Litter quality usually affects decomposition processes, which can, in turn, alter nutrient cycling. Therefore, the change in plant dominance in this ecosystem possibly alters soil carbon and nutrient cycles, and consequently, ecosystem services (e.g. biodiversity conservation, groundwater recharge, …). We hypothesise that, because of its higher litter quality, nutrient turnover becomes faster with grass encroachment. We tested this hypothesis in a field set-up consisting of 14 plots presenting a gradient of increasing grass dominance (from 0% to 100%). We measured nine soil parameters and assessed possible associations between grass dominance and the soil parameters using multivariate analysis and linear mixed models. We found that grass dominance significantly impacted net N mineralisation and the root biomass. Our results showed very low net N mineralisation rates (0.09 ± 0.04 mg N (kg soil)−1 day−1) and relative nitrification rates (1.99 ± 0.62%). At high grass levels, acid phosphatase activity was significantly lower than at lower grass percentages. These results show that grass encroachment has a minimal impact on heathland soil biochemistry at this point. Still, we consider that it may take many years to translate a change in litter quality and dynamics into a change in soil functioning.

The mechanismof promoting rhizosphere nutrient turnover for arbuscular mycorrhizal fungi attributes to recruited functional bacterial assembly.

Symbiosis with arbuscular mycorrhizal (AM) fungi improves plant nutrient capture from the soil, yet there is limited knowledge about the diversity, structure, functioning, and assembly processes of AM fungi-related microbial communities. Here, 16S rRNA gene sequencing and metagenomic sequencing were used to detect bacteria in the rhizosphere of Lotus japonicus inoculated with and without AM fungi, and the L. japonicus mutant ljcbx (defective in symbiosis) inoculated with AM fungi in southern grassland soil. Our results show that AM symbiosis significantly increased bacterial diversity and promoted deterministic processes of bacterial community construction, suggesting that mycorrhizal symbiosis resulted in the directional enrichment of bacterial communities. AM fungi promoted the enrichment of nine bacteria, including Ohtaekwangia, Niastella, Gemmatimonas, Devosia, Sphingomonas, Novosphingobium, Opitutus, Lysobacter, Brevundimonas, which are positively correlated with NPK-related parameters. Through a functional identification experiment, we found that six of these genera, including Brevundimonas, Lysobacter, Ohtaekwangia, Sphingomonas, Devosia, and Gemmatimonas, demonstrated the ability to mineralize organophosphate and dissolve inorganic phosphorus, nitrogen, and potassium. Our study revealed that AM fungi can regulate rhizosphere bacterial community assembly and attract specific rhizosphere bacteria to promote soil nutrient turnover in southern grasslands.

The impact of novel azotobacter Bacillus sp. T28 combined sea buckthorn pomace on microbial community structure in paddy soil

Nitrogen (N) fertilizer application is an essential part of agricultural production in order to improve rice yields. However, long-term irrational application and low utilization of N fertilizer have caused a series of environmental problems. Biofertilizer is considered an effective alternative to N fertilizer. In this study, the effect of biofertilizer made of diazotrophic bacteria Bacillus sp. T28 combined with sea buckthorn pomace on the soil N changes and microbial community structure was conducted. Compared to CK, NO3−-N content decreased 33.1%–43.8% and the rate of N2O release decreased 8–26 times under different fertilizer treatments during incubation of 0–7 days. On the contrary, NH4+-N in T28 with or without sea buckthorn pomace treatments increased by 56.5–118.8% during incubation of 7–14 days. The results indicated that this biofertilizer reduced the environmental risk associated with the accumulation of NO3−-N in paddy soil and the release of N2O to the atmosphere and maintained the soil available N supply capacity. Besides, applying Bacillus T28 with sea buckthorn pomace increased the abundance of soil N functional genes such as nifH, narG, nirS, nirK, and nosZ. The 13C-PLFAs results demonstrated that this biofertilizer improves soil microbial community diversity, nutrient turnover rate and ecosystem stability by altering soil pH and total carbon (TC). In conclusion, Bacillus sp. T28 combined with sea buckthorn pomace regulated the indigenous soil microbial community structure and mitigated the environmental risk of conventional N fertilization in agroecosystems.

Assessment of Earthworm Services on Litter Mineralisation and Nutrient Release in Annual and Perennial Energy Crops (Zea mays vs. Silphium perfoliatum)

Abundant and functionally diverse earthworm communities in perennials deliver ecosystem services like increasing nutrient availability by incorporating organic matter. This study aimed to analyse the decomposition of annual and perennial energy crop residues, and the subsequent nutrient release, depending on earthworm functional diversity. In a laboratory experiment, two ecological earthworm groups—anecics (detritivorous Lumbricus terrestris (L.)) and endogeics (geophagous Aporrectodea caliginosa and A. rosea)—were incubated with wilted cup plant (Silphium perfoliatum) and maize (Zea mays) litter for 4 or 8 weeks. Decomposition and consumption rates were calculated. The C, N and P in litter and casts were analysed. Mineralisation was a function of earthworm biomass and the number of individuals. Functional diversity had no beneficial effect. Decomposition was found to be highest in treatments with detritivorous earthworms, i.e., higher earthworm biomass, yet consumption and nutrient turnover in relation to earthworm biomass were higher in treatments with geophages indicating enhanced competition. N limitation became apparent in both litter treatments and was predominant with cup plant litter. N limitation and recalcitrant cell wall compounds affected consumption rates and the egestion of total C and P. While N in casts was lower than expected, P was increased. We observed that the effects manifested at different stages of decomposition of maize and cup plant, highlighting differences in temporal development of decomposition and nutrient turnover between litter types. Our results indicate that earthworms promote decomposition of recalcitrant litter and nutrient turnover, but N limitation may hamper nutrient release. Cup plant systems offer a suitable habitat for soil-dwelling organisms, but management approaches must consider the adequate input of organic matter as an energy and nutrient source to enhance ecosystem service provision.

Decomposition Characteristics of Lignocellulosic Biomass in Subtropical Rhododendron Litters under Artificial Regulation.

The nutrient turnover of subtropical rhododendron forests is slow, natural regeneration is difficult, and the decomposition of litter is slow. Lignin, cellulose, and hemicellulose are the key factors affecting the decomposition rate of litters. In this study, the litters of three forest stands, namely evergreen broadleaf Rhododendron delavayi, evergreen broadleaf Rhododendron agastum, and deciduous broadleaf mixed forest, were taken as the research objects to explore the dynamic changes and effects of lignin, cellulose, and hemicellulose contents in litters of different stands under indoor artificial control measures. Exogenous nitrogen, phosphorus, alkaline substances, and microbial agents were added to decompose litters in the laboratory for 140 days. Our results showed that (1) the contents of lignin and cellulose in the litters of the three stands decreased significantly in the early stage of decomposition and the content of hemicellulose was stable, and (2) low concentrations of nitrogen and phosphorus can accelerate the degradation of lignin, cellulose, and hemicellulose in litters of the three stands and thus promote the decomposition of litters. This study provides basic data for the nutrient return of artificial intervention in subtropical rhododendron forests in China.

Rhizosphere phage communities drive soil suppressiveness to bacterial wilt disease

BackgroundBacterial viruses, phages, play a key role in nutrient turnover and lysis of bacteria in terrestrial ecosystems. While phages are abundant in soils, their effects on plant pathogens and rhizosphere bacterial communities are poorly understood. Here, we used metagenomics and direct experiments to causally test if differences in rhizosphere phage communities could explain variation in soil suppressiveness and bacterial wilt plant disease outcomes by plant-pathogenic Ralstonia solanacearum bacterium. Specifically, we tested two hypotheses: (1) that healthy plants are associated with stronger top-down pathogen control by R. solanacearum-specific phages (i.e. ‘primary phages’) and (2) that ‘secondary phages’ that target pathogen-inhibiting bacteria play a stronger role in diseased plant rhizosphere microbiomes by indirectly ‘helping’ the pathogen.ResultsUsing a repeated sampling of tomato rhizosphere soil in the field, we show that healthy plants are associated with distinct phage communities that contain relatively higher abundances of R. solanacearum-specific phages that exert strong top-down pathogen density control. Moreover, ‘secondary phages’ that targeted pathogen-inhibiting bacteria were more abundant in the diseased plant microbiomes. The roles of R. solanacearum-specific and ‘secondary phages’ were directly validated in separate greenhouse experiments where we causally show that phages can reduce soil suppressiveness, both directly and indirectly, via top-down control of pathogen densities and by alleviating interference competition between pathogen-inhibiting bacteria and the pathogen.ConclusionsTogether, our findings demonstrate that soil suppressiveness, which is most often attributed to bacteria, could be driven by rhizosphere phage communities that regulate R. solanacearum densities and strength of interference competition with pathogen-suppressing bacteria. Rhizosphere phage communities are hence likely to be important in determining bacterial wilt disease outcomes and soil suppressiveness in agricultural fields.9CDhWVdEuw3PsngifdxC8SVideo

Ufmylation reconciles salt stress-induced unfolded protein responses via ER-phagy in Arabidopsis.

In plants, the endomembrane system is tightly regulated in response to environmental stresses for maintaining cellular homeostasis. Autophagosomes, the double membrane organelles forming upon nutrient deprivation or stress induction, degrade bulky cytosolic materials for nutrient turnover. Though abiotic stresses have been reported to induce plant autophagy, few receptors or regulators for selective autophagy have been characterized for specific stresses. Here, we have applied immunoprecipitation followed by tandem mass spectrometry using the autophagosome marker protein ATG8 as bait and have identified the E3 ligase of the ufmylation system Ufl1 as a bona fide ATG8 interactor under salt stress. Notably, core components in the ufmylation cascade, Ufl1 and Ufm1, interact with the autophagy kinase complexes proteins ATG1 and ATG6. Cellular and genetic analysis showed that Ufl1 is important for endoplasmic reticulum (ER)-phagy under persisting salt stress. Loss-of-function mutants of Ufl1 display a salt stress hypersensitive phenotype and abnormal ER morphology. Prolonged ER stress responses are detected in ufl1 mutants that phenocopy the autophagy dysfunction atg5 mutants. Consistently, expression of ufmylation cascade components is up-regulated by salt stress. Taken together, our study demonstrates the role of ufmylation in regulating ER homeostasis under salt stress through ER-phagy.

Mineral weathering is linked to microbial priming in the critical zone

Decomposition of soil organic matter (SOM) can be stimulated by fresh organic matter input, a phenomenon known as the ‘priming effect’. Despite its global importance, the relationship of the priming effect to mineral weathering and nutrient release remains unclear. Here we show close linkages between mineral weathering in the critical zone and primed decomposition of SOM. Intensified mineral weathering and rock-derived nutrient release are generally coupled with primed SOM decomposition resulting from “triggered” microbial activity. Fluxes of organic matter products decomposed via priming are linearly correlated with weathering congruency. Weathering congruency influences the formation of organo-mineral associations, thereby modulating the accessibility of organic matter to microbial decomposers and, thus, the priming effect. Our study links weathering with primed SOM decomposition, which plays a key role in controlling soil C dynamics in space and time. These connections represent fundamental links between long-term lithogenic element cycling (= weathering) and rapid turnover of carbon and nutrients (= priming) in soil.

Effects of Fungi on Soil Organic Carbon and Soil Enzyme Activity under Agricultural and Pasture Land of Eastern Türkiye

Soil organic matter (SOM) is a heterogeneous mixture of materials ranging from fresh plant tissues to highly decomposed humus at different stages of decomposition. Soil organic carbon (SOC) status is directly related to the amount of organic matter in soil and therefore is generally used to measure it. Soil carbon sequestration refers to the removal of carbon (C) containing substances from the atmosphere and its storage in soil C pools. The soil microbial community (SMC) plays an important role in the C cycle, and its activity is considered to be the main driver of differences in C storage potential in soil. The composition of SMC is crucial for maintaining soil ecosystem services, as the structure and activity of SMC also regulate the turnover and distribution of nutrients, as well as the rate of soil organic matter (SOM) decomposition. Here, we applied fungi on the soils taken from two fields, one used as a pasture and one for agriculture (wheat cultivation), in a histosol in the eastern part of Türkiye and investigated the changes in the organic carbon and enzyme activity contents of the soils at the end of the 41-day incubation period. In the study, four different fungal species (Verticillium dahliae (SOR-8), Rhizoctonia solani (S-TR-6), Fusarium oxysporum (HMK2-6), and Trichoderma sp.) were used and catalase, urease, and alkaline phosphatase activities were examined. Results showed that the values of SOC were V. dahliae (7.46%), Trichoderma sp. (7.27%), R. solani (7.03%), Control (6.97%), and F. oxysporum (6.7%) in pastureland and were V. dahlia (4.72%), control (4.69%), F. oxysporum (4.65%), R. solani (4.37%), and Trichoderma sp. (4.14%) in agricultural land, respectively. SOC and soil enzyme activities were significantly affected by land use types (p < 0.05). The higher SOC and enzyme activities were observed in pastureland. Finally, it was determined that soil organic carbon and soil enzyme activities were affected by fungi. This study is important in terms of revealing that the effects of fungi on soil organic carbon and enzyme activities are different in various land types.

Mycorrhizal fungi alter root exudation to cultivate a beneficial microbiome for plant growth

Abstract Arbuscular mycorrhizal (AM) fungi traditionally form symbioses with most plant species. Although AM fungi have critical effects on microbial communities, the pathways showing how AM fungi shape rhizosphere bacterial communities and their functions are rarely explored. Here, through three systematic experiments, AM fungi–bacteria interactions were first investigated in the rhizosphere of Lotus japonicus, then the interactions were confirmed by a second experiment with wild‐type and a mycorrhiza‐defective mutant ljcbx of L. japonicus. The mechanisms were presented by adding core bacteria and AM fungi to the plant rhizosphere in the third experiment. We found that AM fungi–bacteria interactions enhanced host plant growth and identified a core bacterial group that uniquely enhanced host plant growth. Adding core bacteria and AM fungi promoted host growth and nutrient acquisition compared to adding AM fungi or core bacteria independently. Allelopathic substances secreted by AM fungal colonizing host roots to recruit the rhizosphere bacteria were detected by the multi‐omics joint analysis, showing that arachidonic acid was the main allelopathic substance that affected AM fungi–bacteria interactions. Our findings provide direct evidence that mycorrhizal infection simulated root exudation, such as arachidonic acid, recruited a beneficial microbiome to the host rhizosphere, increasing plant growth and soil nutrient turnover. Read the free Plain Language Summary for this article on the Journal blog.