Background : Numerous studies have been published on the anatomy and physiology of the corpus callosum (CC). There are many contradictory studies in the literature about the changes in the size of the CC by handedness, sex, and age. Objective : This study was carried out to examine the morphometric influence of these factors (handedness, sex, and age) on the CC in healthy Turkish adults Methods : Two hundred thirty-seven normal individuals, 101 males, and 136 females, divided into 5 age groups (20-29, 30-39, 40-49, 50-59, and 60-69) were subjected to magnetic resonance imaging (MRI) study of the brain. Of the 237 subjects, 27 males and 36 females were left-handed. Midsagittal corpus callosum area (CCA) was represented by the mean ± standard deviation of the age groups in square centimeters, and also comparisons of the right- and left-handed were also represented in Table 1. For statistical evaluation, the SPSS 15.0 was used. All statistical tests were set at a 95% confidence level (p 0.05) (Table 2). Conclusion: The results showed no statistical significance of the effect of age or handedness on the surface area of the CC. However, it was statistically significant that the size of men's CC was higher than that of women. Keywords: Midsagittal corpus callosum area, modified Cavalieri method, handedness, Turkish adults, magnetic resonance imaging.