Abstract In order to understand the interaction between fluid and particles, a two-way coupled three-dimensional mixing layer laden with particles at a Stokes number of 5 with different mass loadings is numerically studied. The pseudospectral method is used for the flow fluid and the Lagrangian approach is used to trace particles. The concept of computational particles is introduced to vary the mass loading of particles. The momentum coupling effect introduced by a particle is approximated to the point force. The simulation results show that the coherent structures are still dominant in the mixing layer, but the flow field is modulated by particles. The addition of the particles enhances the energy of all the Fourier modes with non-zero spanwise wavenumber, and the enhancement increases with the augment of the mass loading. A higher mass loading results in a lower energy at fundamental wavenumber and streamwise subharmonic Fourier mode of the fluidin the phase of Kelvin-Helmholtz rolling up. but for large...