This paper reports the results of gyrokinetic simulation studies of ion temperature gradient driven turbulence which investigate the role of non-resonant modes in turbulence spreading, turbulence regulation, and self-generated plasma rotation. Non-resonant modes, which are those without a rational surface within the simulation domain, are identified as nonlinearly driven, radially extended convective cells. Even though the amplitudes of such convective cells are much smaller than that of the resonant, localized turbulence eddies, we find from bicoherence analysis that the mode-mode interactions in the presence of such convective cells increase the efficiency of turbulence spreading associated with nonlocality phenomena. Artificial suppression of the convective cells shows that turbulence spreading is reduced, and that the turbulence intensity profile is more localized. The more localized turbulence intensity profile produces stronger Reynolds stress and E × B shear flows, which in turn results in more effective turbulence self-regulation. This suggests that models without non-resonant modes may significantly underestimate turbulent fluctuation levels and transport.