Sulfate reduction is a commonly reported process in a variety of continental margin settings that affects the carbon (C), sulfur (S), and iron (Fe) marine sedimentary cycles. However, sulfate reduction in unstable environment (turbidite deposition) is easily ignored, which results in a lack of understanding of the C, S, and Fe biogeochemical cycle. Therefore, to elucidate the role of turbidite sedimentation in the global C, S, and Fe biogeochemical cycle, total organic carbon (TOC), total sulfur (TS), iron speciation, pyrite contents, and sulfur isotopic composition in sediments (CL94) on the South China Sea continental slope were analyzed, along with sediment grain sizes, calcium carbonate (CaCO3) content, and K/Al and Ti/Al ratios. Based on petrographic characteristics, grain size parameters, and element geochemistry (K/Al and Ti/Al ratio), it was concluded that the mid-section (322–350 cm) of Site CL94 is a turbidite layer. Compared with normal sedimentary environment, δ34SCRS (−39.7 ‰ on average) exhibits a high value in turbidite layer, which could be related to the high deposition rate and relatively closed environment caused by the turbidity current deposition. Furthermore, in situ deposition is virtually entirely unaffected by external influences, and this is the primary source of sulfide and reactive iron in the relatively closed environment. Thus, pyrite concentration in the turbidity layer fluctuates in conjunction with variations in sulfide and active iron. Moreover, the identification and data extraction of methane seeps were further complicated by the fact that the TS/TOC ratio and δ34SCRS features induced by the turbidity current were extremely similar to those of sediments around methane seeps. The examination of pyrite sulfur isotopic composition and TS/TOC ratios for detecting methane seepage requires further investigation.
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