In order to reveal the acceleration response law of shallow-buried bias double-arch tunnel under earthquake. The acceleration response of the shallow-buried biased double-arch tunnel under the action of Wenchuan wave was studied by the combination of shaking table test and numerical simulation. The effects of non-bias and bias on the acceleration response of shallow-buried biased double-arch tunnels were discussed. Research indicates: (1) Horizontal acceleration amplification factor of the left-hole (non-biased side) for the shallow-buried biased double-arch tunnel is V-shaped, and the right-hole (bias side) is ∩-shaped. The vertical acceleration amplification factor of the left-hole tends to increase continuously, and the right-hole shows a trend of decreasing first and then increasing continuously. (2) In the state of both the bias and the non-biased tunnel, the acceleration response in the vertical direction is stronger than that in the horizontal direction. (3) In the two states of bias and non-bias, the difference between the horizontal and vertical acceleration amplification factors of the double-arch tunnel is larger. It indicates that the excitation direction of the seismic wave has a great influence on the acceleration response of the double-arch tunnel. (4) Relatively biased double-arch tunnels, the regularity of non-biased double-arch tunnels is better, indicating that the bias voltage has a greater impact on double-arch tunnels.