Flexibility and robust performance have made the FOPID (Fractional Order PID) controllers a better choice than PID (Proportional, Integral, Derivative) controllers. But the number of tuning parameters decreases the usage of FOPID controllers. Using synthetic data in available FOPID tuners leads to abnormal controller performances limiting their applicability. Hence, a new tuning methodology involving real-time data and overcomes the drawbacks of mathematical modeling is the need of the hour. This paper proposes a novel FOPID controller tuning methodology using machine learning algorithms. Feed Forward Back Propagation Neural Network (FFBPNN), Multi Least Squares Support Vector Regression (MLSSVR) chosen to design Machine Learning based Optimal Tuner (MLOT) can handle the interdependency between the controller parameters and multiple outputs for multiple inputs.The proposed tuner finds application in the control of power and energy systems. It can accomplish tracking, disturbance-rejection, and robustness controller performances, thus making FOPID controller design easier and accurate. Comparisons with existing FOPID tuning rules show better controller performances and easy tuning. Thus, this paper addresses a unique, real-time, model-free, easily tunable FOPID tuning methodology satisfying plant requirements.