Aiming at the problems how to realize long range, high speed identification and how to integrate web with the management system in the UHF RFID applications, a UHF RFID reader system based on S3C2440 microprocessor and AS3992 UHF Reader chip is designed and implemented in this paper. By designing the RF front-end power amplifier and signal isolation circuit, the transmit power detection circuit and the antenna impedance tunable matching circuit, the transmit power reaches up to 32 dBm and the receiver SNR is improved at the same time, resulting in the stable read range reaching up to 12 meters. In order to integrate web control into the RFID management system, the reader system is transplanted into the embedded Linux OS and a web management system that is based on web server and CGI is constructed. Device drivers are developed and RFID APIs are packaged, greatly facilitating the software development and maintenance. By using the Random-Slotted anti-collision algorithm, high speed identification of ISO18000-6C RFID tags has been achieved.