Event Abstract Back to Event Possible involvement of alterations in tumor necrosis factor beta secretions in the metformin-induced suppression of the proliferation in P19 cells Nobuyuki Kuramoto1*, N Nitta1, H Higashi1 and T Kinjo1 1 Setsunan University, Laboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Japan Neural stem/progenitor cells (NPCs), which are defined by their ability to self-renew and differentiate into 3 major cell types including neurons, astrocytes, and oligodendrocytes, play a key role in the development and maturation of the central nervous system. P19 embryonic carcinoma cells resemble NPCs. It has been reported that 5’-AMP-activated protein kinase (AMPK) acts not only as an efficient sensor for cellular ATP level, but as a regulator for cellular proliferation and differentiation. Here, we measured cytokine expressions in P19 cells under the condition of the metformin-induced suppression of the proliferation. P19 cells were cultured in alpha minimum essential medium in a 5% CO2 humidified chamber at 37°C. After plating P19 cells at a concentration of 3.0×105 cells/mL, at every 12 h, cell viability was measured by abilities of MTT reduction and gene expression was semi-quantified by RT-PCR. P19 cells grew and reached confluence within 48 h after the plating. Metformin suppressed the proliferation of P19 cells in a concentration-dependent manner. Among several cytokines, gene expression of tumor necrosis factor (TNF)-beta increased by metformin in a concentration dependent manner. It was therefore suggested that metformin-induced suppression of the proliferation in P19 cells might involve the alteration of the gene expressions and secretions of TNF-beta. Keywords: Cytokines, Gene Expression, in vitro, neural progenitor cells, P19 cells Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: Poster Presentation Session Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Kuramoto N, Nitta N, Higashi H and Kinjo T (2016). Possible involvement of alterations in tumor necrosis factor beta secretions in the metformin-induced suppression of the proliferation in P19 cells. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00115 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Prof. Nobuyuki Kuramoto, Setsunan University, Laboratory of Molecular Pharmacology, Faculty of Pharmaceutical Sciences, Hirakata, Osaka, 573-0101, Japan, kuramoto@pharm.setsunan.ac.jp Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Nobuyuki Kuramoto N Nitta H Higashi T Kinjo Google Nobuyuki Kuramoto N Nitta H Higashi T Kinjo Google Scholar Nobuyuki Kuramoto N Nitta H Higashi T Kinjo PubMed Nobuyuki Kuramoto N Nitta H Higashi T Kinjo Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.