Tumor Necrosis Factor-α-induced Protein 8-like 2 Expression Research Articles

The Effect of Tumor Necrosis Factor-α-Induced Protein 8 Like-2 on Immune Response of CD4+T Lymphocytes in Mice after Thermal Injury

The status of cellular immunity has been shown to be associated with the occurrence and development of sepsis. Accumulating evidence has demonstrated that tumor necrosis factor-α-induced protein 8 like-2 (TIPE2) plays an important role in maintaining homeostasis of immune function. The present study, with the use of a controlled in vivo approach, demonstrated the effect of TIPE2 on cell-mediated immunity of CD4+ T lymphocytes in thermal injury murine model. One hundred and twenty-eight male mice were randomly allocated into four groups, which were sham burn group (n=48), burn group (n=48), burn with lentivirus-RNAi-TIPE2 transfection group (n=16), burn with negative control transfection group (n=16), and they were sacrificed at the designated time points. CD4+ T lymphocytes were isolated from the spleen using MACS microbeads. Phenotypes were analyzed by flow cytometry analysis, and cytokines were determined using ELISA kits. We found that the expression of TIPE2 was markedly increased in CD4+T lymphocytes in mice at 24,48 and 72 hours postburn. Down-regulation of TIPE2 by lentivirus-RNAi-TIPE2 attenuated the suppressive effect of CD4+T lymphocytes, which was associated with profound elevation of nuclear factor of activated T cell (NF-AT) activity. These results demonstrate that TIPE2 appear to be involved in the immune regulation of CD4+ T lymphocytes, and the decrease in TIPE2 expression on CD4+T lymphocytes in vivo can enhance peripheral T lymphocyte function after thermal injury. These data might provide a valid strategy to prevent the development of immunosuppressive state resulted from major burns.

Down-regulation of TIPE2 expression in peripheral blood mononuclear cells from patients with asthma in childhood

The breakdown of immune homeostasis contributes to the pathogenesis of asthma. Tumor necrosis factor α induced protein-8 like-2 (TIPE2) is an immune homeostasis maintaining gene identified recently. Wondering whether TIPE2 is associated with asthma, we examined the TIPE2 (in mRNA and protein levels) and FCER1A (in mRNA levels) in peripheral blood mononuclear cells (PBMC) from 37 asthma patients and 34 healthy controls by real-time reverse transcription-polymerase chain reaction analysis (RT-PCR) and Western blot analysis. The TIPE2 mRNA expression levels were significantly down-regulated in asthma patients compared with healthy controls (P<0.001), while the FCER1A mRNA expression levels was increased in asthma patients compared with healthy controls (P=0.008). Furthermore, the TIPE2 mRNA expression levels correlate with IgE negatively (r=−0.433, P=0.007), the FCER1A mRNA expression levels correlate with IgE positively (r=0.658, P<0.001) and the TIPE2 mRNA expression levels correlate with FCER1A mRNA expression levels negatively (r=−0.423, P=0.009) in all asthma patients. The TIPE2 protein expression levels were also down-regulated significantly in asthma patients compared with healthy controls (P=0.019). These results indicate that the decreased TIPE2 is associated with asthma closely and suggest TIPE2 is an important gene in the pathogenesis of asthma.