Tufts University Research Articles

Assessment of implementation of the caries management by risk assessment philosophy in a dental school.

This cross-sectional study assessed the implementation of documenting a baseline caries risk assessment (CRA) of patients seen by predoctoral dental students and its association with the presence of caries risk management (CRM) treatment. A convenience sample of 10,000 electronic axiUm patient records at Tufts University School of Dental Medicine was retrospectively assessed for the presence or absence of a completed CRA and CRM after IRB approval following predetermined inclusion and exclusion criteria. The CRM variables (nutrition counseling, sealant, fluoride) were identified by procedure codes that were completed by the student. Associations were assessed via the chi-square test, Kruskal-Wallis test (with Dunn's test and the Bonferroni correction used in post-hoc tests) and Mann-Whitney U test. Most patients (70.5%) had a CRA completed. However, only 24.9% (out of the 7045 patients with a completed CRA) received CRM, while 22.9% of the 2,955 patients without a CRA received CRM. The difference between the groups with and without a completed CRA in terms of the percentage receiving CRM was not clinically significant. Significant associations were found between a completed CRA and in-house fluoride treatment (p=.034) and between a completed CRA and sealant treatment (p=.001). Patients with higher baseline CRA levels (i.e., greater risk) were more likely to have CRM (16.9% of the 785 patients at low risk, 21.1% of the 1282 patients at moderate risk, 26.3% of the 4347 patients at high risk, and 32.6% of the 631 patients at extreme risk). The association between these two variables was significant (p<.001). There is evidence that students were mostly compliant with completing a CRA for most patients; however, there is a deficiency in implementation of CRM approach to help support dental caries management, and there is still much room for improvement.

Demonstrating the importance of local culture and susceptibility data: antibiograms from dogs at a veterinary tertiary care center.

To create antibiograms for commonly cultured organisms in a small animal tertiary care hospital following Clinical and Laboratory Standards Institute guidelines and to compare these local resistance patterns to published first-tier antimicrobial recommendations. Urine (n = 429), respiratory (41), and skin (75) isolates cultured from dogs between January 1, 2019, and December 31, 2020, at the Tufts University Foster Hospital for Small Animals. MIC and susceptibility interpretations were recorded for multiple sites for 2 years. Sites with greater than 30 isolates for at least 1 organism were included. Urinary, respiratory, and skin antibiograms were created using Clinical and Laboratory Standards Institute breakpoints and guidelines. Urinary Escherichia coli had a higher susceptibility percentage for amoxicillin-clavulanate (80% [221/275]) than amoxicillin alone (64% [175/275]). Respiratory E coli were greater than 80% susceptible to only 2 antimicrobials (imipenem, amikacin). Of skin Staphylococcus pseudintermedius isolates, 40% (30/75) were methicillin-resistant and frequently also displayed resistance to non-beta lactam antimicrobials. Susceptibility to recommended first-line antimicrobials varied and was greatest for gram-negative urinary isolates and lowest for methicillin-resistant S pseudintermedius skin isolates and respiratory E coli. Local antibiogram creation identified frequent resistance that may preclude the use of guideline-recommended first-line therapy. High levels of resistance identified in methicillin-resistant S pseudintermedius isolates supports growing concern for methicillin-resistant staphylococci in veterinary patients. This project highlights the need for population-specific resistance profiles to be used in conjunction with national guidelines.

Iterative optimal sensor placement for adaptive structural identification using mobile sensors: Numerical application to a footbridge

This paper proposes an iterative optimal sensor placement (OSP) framework for structural identification and model updating of structural systems using a small number of mobile sensors. The model updating is performed through a Bayesian inference approach which is solved through asymptotic approximation for computational efficiency. In an iterative manner, the OSP is performed to minimize the information entropy in estimating the updating parameters of the model. Each OSP iteration is performed to find the next location of mobile sensors, where the prior probability distribution of updating parameters is assumed as the posterior probability distribution obtained from the previous iteration. This process is repeated until the uncertainties of updating parameters fall below a predetermined threshold. A forward sequential sensor placement algorithm is used to solve the OSP problem at each iteration. This algorithm provides a nearly-optimal solution and is much more efficient compared to an exhaustive search. This proposed framework is applied to a numerical case study, namely the Dowling Hall Footbridge located at Tufts University campus. Updating parameters used in this study are the added mass at different segments of the bridge deck. The purpose of using added mass is to create a realistic pseudo damage on a specific portion of the bridge. The proposed iterative system identification approach is applied for estimation of updating parameters considering different number of available sensors. This study shows that the iterative OSP approach using a small number of mobile sensors placed iteratively provides better model updating results compared to the case of using an optimal static sensor configuration involving larger number of sensors.

Benchmarking Patient Engagement Capabilities and Preparedness of Drug Development Sponsors.

Consistent implementation and measurement of patient engagement initiatives across the industry have remained aspirational and elusive despite strong interest in adopting patient-centric approaches. One factor contributing to this inertia stems from a lack of standardized implementation of patient engagement activities, which varies widely from company to company, making it difficult to track and measure. Further, empirical evidence mapping the impact of patient engagement capabilities on clinical research outcomes has remained sparse. To address this gap, the Drug Information Association (DIA) and Tufts Center for the Study of Drug Development (Tufts CSDD) at the Tufts University School of Medicine developed and administered an assessment tool that companies can use to not only evaluate their organization's patient engagement capabilities and implementation preparedness but can also measure the impact of such activities on trial outcomes. Results showed that while most organizations are providing logistical support to increase patient engagement in the form of travel stipends, accommodation, and financial incentives, most are not implementing more involved forms of patient engagement such as gathering patient input through patient input panels or patient steering committees. This paper discusses the process for designing and administering this assessment tool, the results of the assessment, and future implications.

Hopewell nabs seed funding for lipid nanoparticle delivery

Massachusetts-based Hopewell Therapeutics closed $25 million in seed funding on June 7. The start-up aims to design lipid nanoparticles (LNPs) for delivering nucleic acid cargos to treat lung diseases. LNPs had a breakout role delivering the cargo in messenger RNA–based COVID-19 vaccines . But Hopewell CEO Louis Brenner says the firm’s founding scientist has been in the business a lot longer, and the “science has been maturing in academia and going into the company for more than a decade.” Hopewell’s founder and chief technological officer, Tufts University’s Qiaobing Xu, got his start in LNPs when he was a postdoctoral researcher under biochemist Robert Langer at the Massachusetts Institute of Technology in the late 2000s. Recently, Xu’s team at Tufts devised LNPs that can ferry nucleic acid payloads to the lungs and brain in mice, discoveries that are foundational to Hopewell’s platform. Research veterans agree that although the field has come

Sack Sackler: the role of medical student activism in creating meaningful change at Tufts University

"Sack Sackler: the role of medical student activism in creating meaningful change at Tufts University." Baylor University Medical Center Proceedings, ahead-of-print(ahead-of-print), p. 1 Disclosure statementThe authors report no funding or conflicts of interests.

Fostering the development of dental educators worldwide: A mini-residency model.

Journal of Dental EducationAccepted Articles ADVANCING THROUGH INNOVATION Fostering the development of dental educators worldwide: A mini-residency model Irina F. Dragan DDS, DMD, MS, eMBA, Corresponding Author Irina F. Dragan DDS, DMD, MS, eMBA [email protected] orcid.org/0000-0002-4045-8564 Adjunct Associate Professor of Periodontology, Tufts University School of Dental Medicine, Boston - MA, USA, Lecturer on Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA, USA Correspondence Dr. Irina F. Dragan, Tufts University School of Dental Medicine, Department of Periodontology, One Kneeland Street, Boston - MA, 02111. Email: [email protected]Search for more papers by this authorTahir Hamza BDS, MS, Tahir Hamza BDS, MS Department of Periodontics and Oral Medicine, Resident PGY-1, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USASearch for more papers by this authorGabriela Lagreca DDS, MBA, Gabriela Lagreca DDS, MBA Director of Faculty Education & Calibration and Assistant Professor of Prosthodontics, Tufts University School of Dental Medicine, Boston, MA, USASearch for more papers by this authorNadeem Y. Karimbux DMD, MMSc, Nadeem Y. Karimbux DMD, MMSc Dean and Professor of Periodontology, Tufts University School of Dental Medicine, Boston, MA, USASearch for more papers by this author Irina F. Dragan DDS, DMD, MS, eMBA, Corresponding Author Irina F. Dragan DDS, DMD, MS, eMBA [email protected] orcid.org/0000-0002-4045-8564 Adjunct Associate Professor of Periodontology, Tufts University School of Dental Medicine, Boston - MA, USA, Lecturer on Oral Medicine, Infection, and Immunity, Harvard School of Dental Medicine, Boston, MA, USA Correspondence Dr. Irina F. Dragan, Tufts University School of Dental Medicine, Department of Periodontology, One Kneeland Street, Boston - MA, 02111. Email: [email protected]Search for more papers by this authorTahir Hamza BDS, MS, Tahir Hamza BDS, MS Department of Periodontics and Oral Medicine, Resident PGY-1, University of Pennsylvania School of Dental Medicine, Philadelphia, PA, USASearch for more papers by this authorGabriela Lagreca DDS, MBA, Gabriela Lagreca DDS, MBA Director of Faculty Education & Calibration and Assistant Professor of Prosthodontics, Tufts University School of Dental Medicine, Boston, MA, USASearch for more papers by this authorNadeem Y. Karimbux DMD, MMSc, Nadeem Y. Karimbux DMD, MMSc Dean and Professor of Periodontology, Tufts University School of Dental Medicine, Boston, MA, USASearch for more papers by this author First published: 28 April 2023 https://doi.org/10.1002/jdd.13237 This article has been accepted for publication and undergone full peer review but has not been through the copyediting, typesetting, pagination and proofreading process, which may lead to differences between this version and the Version of Record. Please cite this article as https://doi.org/10.1002/jdd.13237 AboutPDF ToolsExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Accepted ArticlesAccepted, unedited articles published online and citable. The final edited and typeset version of record will appear in the future. RelatedInformation

Bibliometric Map of Educational Robotics Studies

The purpose of this study is to determine the general trend of research in the field of educational robotics through bibliometric analysis. 1382 papers indexed in the WOS database between 1975-2021 were subjected to bibliometric analyses. The data of the study were analyzed using VOSviwer and SciMAT. At the end of the study, it has been concluded that in the field of educational robotics the most productive country was the USA, the most influential journal was Computers and Education, the most influential author was Bers, M.U., and the most influential institution was Tufts University. It has been also concluded that computational thinking, STEM, coding, programming, social robots, and communication themes have become a trend in the field of educational robotics in recent years.

Holy Object No. 1

May 01 2023 Holy Object No. 1 Pardis Alipour Pardis Alipour Pardis Alipour is an artist currently pursuing her MFA at the School of the Museum of Fine Arts at Tufts University. She received a bachelor's degree in Architecture from the University of Tehran. Through incorporating the tools and skills collected from architecture school, she activates spaces, objects, and materials to analyze, criticize, and physicalize the stratified experience of living under an Islamic theocracy. Search for other works by this author on: This Site Google Scholar Author and Article Information Pardis Alipour Pardis Alipour is an artist currently pursuing her MFA at the School of the Museum of Fine Arts at Tufts University. She received a bachelor's degree in Architecture from the University of Tehran. Through incorporating the tools and skills collected from architecture school, she activates spaces, objects, and materials to analyze, criticize, and physicalize the stratified experience of living under an Islamic theocracy. Online ISSN: 2572-7338 Print ISSN: 1091-711X © 2023 Pardis Alipour2023Pardis Alipour Thresholds (2023) (51): 136–137. https://doi.org/10.1162/thld_a_00798 Cite Icon Cite Permissions Share Icon Share Facebook Twitter LinkedIn MailTo Views Icon Views Article contents Figures & tables Video Audio Supplementary Data Peer Review Search Site Citation Pardis Alipour; Holy Object No. 1. Thresholds 2023; (51): 136–137. doi: https://doi.org/10.1162/thld_a_00798 Download citation file: Ris (Zotero) Reference Manager EasyBib Bookends Mendeley Papers EndNote RefWorks BibTex toolbar search Search Dropdown Menu toolbar search search input Search input auto suggest filter your search All ContentAll JournalsThresholds Search Advanced Search This content is only available as a PDF. © 2023 Pardis Alipour2023Pardis Alipour Article PDF first page preview Close Modal You do not currently have access to this content.

Identifying Racial Inequities in Respect and Autonomy During Delivery Care [ID: 1378253

INTRODUCTION: Measuring patient experience is core to understanding and addressing inequities in obstetric care quality. METHODS: People who gave birth at one of five hospitals in Massachusetts and identified as non-Hispanic Black (NHB) or non-Hispanic White (NHW) were invited to complete a structured survey 7–16 weeks postpartum, from February to September 2022. The survey included two validated patient experience instruments: the Mothers on Respect index, dichotomized as “very respectful” or not and the Mothers Autonomy in Decision Making scale, dichotomized as “high patient autonomy” or not. We report risk ratios of associations between patient characteristics and respectful or autonomous care, adjusting for hospital cluster using Stata v.17. Institutional review board approval was received from the Social and Behavioral Research IRB at Tufts University. RESULTS: Of 417 respondents (response rate: 25%), 245 (59%) were NHW and 172 (41%) were NHB. Most had a bachelor’s degree (62%). 241 (60%) of births were vaginal. Very respectful treatment was reported by 78% of respondents, whereas high patient autonomy was only reported by 64%. Non-Hispanic White people were 1.2 times as likely to report high respect compared to NHB (95% CI: 1.1, 1.4). Respect was also significantly lower for those with lower education and those with vaginal birth compared to those with cesarean birth. Differences in respect by race remained significant in multivariable models (1.2, 95% CI: 1.0, 1.4). Autonomy did not differ by any of the assessed demographic or delivery characteristics. CONCLUSION: Examining patient experience revealed inequities. To identify opportunities for improvement and monitor change, brief, validated, patient reported experience measures need to be incorporated into routine data collection.

Class participation correlates with academic performance in a Biochemistry and Metabolism course for first-year veterinary school students

The objective of the current study was to investigate whether class participation correlates with academic performance in a Biochemistry and Metabolism Course for first-year veterinary school students. Veterinary Biochemistry and Metabolism is a required course for first-year veterinary students at the Cummings School of Veterinary Medicine at Tufts University. The course is a large lecture course with approximately 100 students enrolled each year. Topics covered in the course include the metabolism of carbohydrates, nucleic acids, lipids, proteins, vitamins, and microminerals. In the fall of 2021, students had three options for lecture attendance. Students could attend in person, could listen to lectures streamed live, or could watch lectures at a later time since class sessions were all recorded. To increase engagement in the course, students receive Poll Everywhere questions during most of the lectures in this course. These questions were mainly in multiple-choice format and delivered to students at various times (beginning, middle, and end) during the class. Students who earned A, B, or C grades were compared with how often those cohorts participated in the Poll Everywhere questions. The results indicate that students who earned an A in the course have statistically significant higher participation in Poll Everywhere questions when compared to students who earned a B or C. The results do not distinguish between students who attended the lecture in person and those who watched the live stream since all those students could answer the Poll Everywhere questions during class time. The results demonstrate an association between class participation and academic performance. Namely, higher involvement as measured by answering Poll Everywhere question participation increases the likelihood of getting an A. Many professional schools (medical, dental, and veterinary) routinely record most lectures and do not require attendance. While lecture recordings may provide a valuable study tool for students, these results suggest that students who do not actively engage with class material miss an opportunity to maximize learning. No funding to disclose This is the full abstract presented at the American Physiology Summit 2023 meeting and is only available in HTML format. There are no additional versions or additional content available for this abstract. Physiology was not involved in the peer review process.

A Novel Approach to Haglund Deformity Resection Using a Percutaneous Ultrasound-Guided Osteotomy Device.

1Department of Orthopedics and Rehabilitation, Tufts University, Boston, MA 2Tufts University School of Medicine, Boston, MA 3Private Practice, Boston Sports & Biologics, Wellesley, MA Address for correspondence: Walter I. Sussman, DO, Department of Orthopedics and Rehabilitation, 800 Washington St., Tufts Medical Center, Boston, MA 02111; E-mail: [email protected].

Time to publication of cost-effectiveness analyses for medical devices.

Academic researchers and physicians have called for greater use of cost-effectiveness analyses in informing treatment and reimbursement decisions. This study examines the availability of cost-effectiveness analyses for medical devices, in terms of both the number of studies and when studies are published. Analysis of the number of years between FDA approval/clearance and publication for cost-effectiveness analyses of medical devices in the United States published between 2002 and 2020 (n = 86). Cost-effectiveness analyses of medical devices were identified using the Tufts University Cost-Effectiveness Analysis Registry. Studies in which the model and manufacturer of the medical device used in the intervention were identifiable were linked to FDA databases. Years between FDA approval/clearance and publication of cost-effectiveness analyses were calculated. A total of 218 cost-effectiveness analyses of medical devices in the United States published between 2002 and 2020 were identified. Of these studies, 86 (39.4%) were linked to FDA databases. Studies examining devices approved via premarket approval were published a mean of 6.0 years after the device received FDA approval (median, 4 years), whereas studies examining devices that were cleared via the 510(k) process were published a mean of 6.5 years after the device received FDA clearance (median, 5 years). There are few studies describing the cost-effectiveness of medical devices. Most of these studies' findings are not published until several years after the studied devices received FDA approval/clearance, meaning that decision makers will likely not have evidence of cost-effectiveness when making initial decisions related to newly available medical devices.

ECR Spotlight – Yordano Jimenez and Kelsey Lucas

ECR Spotlight is a series of interviews with early-career authors from a selection of papers published in Journal of Experimental Biology and aims to promote not only the diversity of early-career researchers (ECRs) working in experimental biology during our centenary year, but also the huge variety of animals and physiological systems that are essential for the ‘comparative’ approach. Yordano Jimenez and Kelsey Lucas are authors on ‘ Flexibility is a hidden axis of biomechanical diversity in fishes’, published in JEB. Yordano is a Postdoctoral Research Fellow in the lab of Eric Tytell at Tufts University, Medford, USA, investigating the comparative biomechanics of muscle. Kelsey is Assistant Professor in Biological Sciences, University of Calgary, Canada, investigating the ecological biomechanics of aquatic animals.

Raising global awareness to meet the future needs of dental sleep medicine.

Journal of Dental EducationEarly View ADVANCING THROUGH INNOVATION Raising global awareness to meet the future needs of dental sleep medicine Shibani Sahni BSD, MMSc, Corresponding Author Shibani Sahni BSD, MMSc [email protected] orcid.org/0000-0002-6342-7226 Director of Lifelong Learning and Distance Education and Assistant Professor Department of Comprehensive Care., Tufts University School of Dental Medicine, Massachusetts, USA Correspondence Shibani Sahni, BSD, MMSc, Department of Comprehensive Care, Tufts University School of Dental Medicine, One Kneeland St. Suite 1419B, Boston, MA 02111, USA. Email: [email protected]Search for more papers by this authorNadeem Karimbux DMD, MMS, Nadeem Karimbux DMD, MMS Dean, Professor Periodontology, Tufts University School of Dental Medicine, Boston, Massachusetts, USASearch for more papers by this authorFernanda Almeida DDS, MSc, PhD, Fernanda Almeida DDS, MSc, PhD Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, CanadaSearch for more papers by this authorLeopoldo P. Correa BDS, MS, Leopoldo P. Correa BDS, MS Tufts Craniofacial Pain Center, Boston, Massachusetts, USASearch for more papers by this author Shibani Sahni BSD, MMSc, Corresponding Author Shibani Sahni BSD, MMSc [email protected] orcid.org/0000-0002-6342-7226 Director of Lifelong Learning and Distance Education and Assistant Professor Department of Comprehensive Care., Tufts University School of Dental Medicine, Massachusetts, USA Correspondence Shibani Sahni, BSD, MMSc, Department of Comprehensive Care, Tufts University School of Dental Medicine, One Kneeland St. Suite 1419B, Boston, MA 02111, USA. Email: [email protected]Search for more papers by this authorNadeem Karimbux DMD, MMS, Nadeem Karimbux DMD, MMS Dean, Professor Periodontology, Tufts University School of Dental Medicine, Boston, Massachusetts, USASearch for more papers by this authorFernanda Almeida DDS, MSc, PhD, Fernanda Almeida DDS, MSc, PhD Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, CanadaSearch for more papers by this authorLeopoldo P. Correa BDS, MS, Leopoldo P. Correa BDS, MS Tufts Craniofacial Pain Center, Boston, Massachusetts, USASearch for more papers by this author First published: 06 April 2023 https://doi.org/10.1002/jdd.13214Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article. Early ViewOnline Version of Record before inclusion in an issue RelatedInformation

Diaphragmatic Breathing and Performance Anxiety in Dental Students

Purpose/Objectives. The aims of this study were to assess first-year dental students’ attitudes and perceptions of diaphragmatic breathing (DB) and compare students with and without performance anxiety in terms of their attitudes and perceptions of DB. Methods. A lecture discussing a DB technique was presented to 195 students in the Operative Dentistry course at Tufts University School of Dental Medicine. Post-lecture, a 10-question survey was distributed and addressed students’ attitudes and perceptions towards DB and its potential impact in dental school, personal lives, and future careers. Results. Of the 81 surveys completed, 44 students (54%) reported having heard of DB previously; 59 (73%) reported they experience performance anxiety. Seventy-two students (89%) reported thinking that practicing DB will benefit them as practicing dentists; 71 (88%) reported they would use it if stressed in everyday life. Seventy-six (94%) reported they would be interested in using the technique before a competency exam. Students reporting performance anxiety expressed significantly greater agreement that DB is a valuable coping tool ( P = .016) and willingness to participate in DB before an Operative Dentistry competency exam ( P = .018). Conclusions. Teaching diaphragmatic breathing to first-year dental students may provide them with a valuable coping tool to mitigate stress and anxiety.

Out with the old, in with the new: What's up with Simonsiella spp.?

Veterinary Clinical PathologyEarly View LETTER TO THE EDITOR Out with the old, in with the new: What's up with Simonsiella spp.? Francisco O. Conrado, Corresponding Author Francisco O. Conrado [email protected] orcid.org/0000-0001-5055-2637 Departments of Comparative Pathobiology, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts, USA Correspondence Francisco O. Conrado, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA, USA. Email: [email protected]Search for more papers by this authorNicole I. Stacy, Nicole I. Stacy orcid.org/0000-0003-4258-4414 Comparative, Diagnostic, & Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USASearch for more papers by this authorJames F. X. Wellehan Jr, James F. X. Wellehan Jr Comparative, Diagnostic, & Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USASearch for more papers by this author Francisco O. Conrado, Corresponding Author Francisco O. Conrado [email protected] orcid.org/0000-0001-5055-2637 Departments of Comparative Pathobiology, Cummings School of Veterinary Medicine, Tufts University, North Grafton, Massachusetts, USA Correspondence Francisco O. Conrado, Cummings School of Veterinary Medicine at Tufts University, North Grafton, MA, USA. Email: [email protected]Search for more papers by this authorNicole I. Stacy, Nicole I. Stacy orcid.org/0000-0003-4258-4414 Comparative, Diagnostic, & Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USASearch for more papers by this authorJames F. X. Wellehan Jr, James F. X. Wellehan Jr Comparative, Diagnostic, & Population Medicine, College of Veterinary Medicine, University of Florida, Gainesville, Florida, USASearch for more papers by this author First published: 08 April 2023 https://doi.org/10.1111/vcp.13246Read the full textAboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL No abstract is available for this article. Early ViewOnline Version of Record before inclusion in an issue RelatedInformation

122 DIAMOND 2.0: Updating a Pioneering Digital Platform for Study Team Workforce Development

OBJECTIVES/GOALS: There is a critical need to provide quality training for study teams. Materials need to be flexible and available at the learner’s preferred time and format. DIAMOND was developed to provide nimble education offerings that respond to the changing landscape of clinical and translational research. METHODS/STUDY POPULATION: In 2018 four CTSA institutions (the University of Michigan, the Ohio State University, Rochester University, and Tufts University) collaborated to launch the DIAMOND portal. Developed as a CTSA-wide web-based platform, DIAMOND allows members of clinical and translational research teams to widely share and access training and education resources. In 2022 a MICHR-led update of DIAMOND used principles of user centered design to improve the platform. New features include updated search functions to quickly find and sort training materials, tagging training materials to the characteristics of a translational scientist, and development of user controlled customized playlists. RESULTS/ANTICIPATED RESULTS: DIAMOND currently includes 217 training resources developed by 30 CTSA hubs and private industry. The platform has over 600 page views per day from users across the U.S. and internationally. DIAMOND includes an easy-to-use form to upload new materials to the platform. Contributors are asked to include key words and select competency domains and characteristics of a translational scientist that apply to their materials. Other new features include tagging materials to streamline and improve search results, the ability to sort materials by competency domain or characteristics of a translational scientist, and the ability for users to create and share customized, personalized playlists. DISCUSSION/SIGNIFICANCE: DIAMOND is an important tool to support workforce development for study teams. Updates to the flexible digital platform meet the needs and preferences of adult learners and busy health professionals. Lessons learned from the design process and future plans for the platform will be explored.

Using cultured canine cardiac slices to model the autophagic flux with doxorubicin.

Chemotherapy-induced impairment of autophagy is implicated in cardiac toxicity induced by anti-cancer drugs. Imperfect translation from rodent models and lack of in vitro models of toxicity has limited investigation of autophagic flux dysregulation, preventing design of novel cardioprotective strategies based on autophagy control. Development of an adult heart tissue culture technique from a translational model will improve investigation of cardiac toxicity. We aimed to optimize a canine cardiac slice culture system for exploration of cancer therapy impact on intact cardiac tissue, creating a translatable model that maintains autophagy in culture and is amenable to autophagy modulation. Canine cardiac tissue slices (350 μm) were generated from left ventricular free wall collected from euthanized client-owned dogs (n = 7) free of cardiovascular disease at the Foster Hospital for Small Animals at Tufts University. Cell viability and apoptosis were quantified with MTT assay and TUNEL staining. Cardiac slices were challenged with doxorubicin and an autophagy activator (rapamycin) or inhibitor (chloroquine). Autophagic flux components (LC3, p62) were quantified by western blot. Cardiac slices retained high cell viability for >7 days in culture and basal levels of autophagic markers remained unchanged. Doxorubicin treatment resulted in perturbation of the autophagic flux and cell death, while rapamycin co-treatment restored normal autophagic flux and maintained cell survival. We developed an adult canine cardiac slice culture system appropriate for studying the effects of autophagic flux that may be applicable to drug toxicity evaluations.

P30 - “Don’t worry, you can trust me”: Indicators of the role of trust in residents’ reflective writing: Presenter(s): Allen Shaughnessy, Tufts University School of Medicine, United States; Ashley Duggan, Boston College, United States

P30 - “Don’t worry, you can trust me”: Indicators of the role of trust in residents’ reflective writing: Presenter(s): Allen Shaughnessy, Tufts University School of Medicine, United States; Ashley Duggan, Boston College, United States