You have accessJournal of UrologyInfections/Inflammation/Cystic Disease of the Genitourinary Tract: Kidney & Bladder II1 Apr 2017MP23-01 HUMAN URINE-DERIVED STEM CELLS LESSEN INFLAMMATION AND FIBROSIS WITHIN KIDNEY TISSUE IN A RODENT MODEL OF AGING-RELATED RENAL INSUFFICIENCY Ting Long, Yaodong Jiang, Hua Shi, Liren Zhong, Deying Zhang, Wei Li, Yong Zhang, Dong Chen, Yan Jiao, Debra Diz, and Yuanyuan Zhang Ting LongTing Long More articles by this author , Yaodong JiangYaodong Jiang More articles by this author , Hua ShiHua Shi More articles by this author , Liren ZhongLiren Zhong More articles by this author , Deying ZhangDeying Zhang More articles by this author , Wei LiWei Li More articles by this author , Yong ZhangYong Zhang More articles by this author , Dong ChenDong Chen More articles by this author , Yan JiaoYan Jiao More articles by this author , Debra DizDebra Diz More articles by this author , and Yuanyuan ZhangYuanyuan Zhang More articles by this author View All Author Informationhttps://doi.org/10.1016/j.juro.2017.02.729AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookTwitterLinked InEmail INTRODUCTION AND OBJECTIVES Kidney function declines with age; older adults have renal function only 50-60% that of healthy young people. Most older patients have some degree of impaired renal function after renal surgery. Cell-based therapy is a promising alternative method to restore renal function in the treatment of chronic renal insufficiency. The goal of this study is to determine whether human urine-derived stem cells (USCs) can prompt renal tissue remolding in a rodent model of age-related kidney insufficiency. METHODS Urine samples were obtained from healthy men (n=6, 28-35 years old). USCs were isolated and expanded at passage 5. Eighteen male SD rats (50-62 week old) with renal insufficiency (increased levels of urine protein and serum creatinine) were divided into 3 groups (G). G1, animals received 5 intravenous cell implantations of 2 x106 cells/0.2 ml serum-free medium /rat/time period weekly; G2, as in G1 but intraperitoneally delivered; G3, as in G1 but serum-free medium alone. Healthy male SD rats at the same age were controls. RESULTS Cultured USCs secreted >20 kinds of trophic factors, including high levels of matrix metallopeptidase 9, which is involved in extracellular matrix degradation. Histologic and immunocytochemical staining showed that ED1 (inflammatory marker), alpha-smooth muscle actin (myofibroblast marker), and collagen deposition were significantly less within the medulla or cortex in rats with renal insufficiency vs. controls. G1 and G2 rats showed significantly inhibited inflammation and fibrosis in interstitial tissue, and reduced collagen deposits around renal vessels and the basement membrane of renal tubule and glomerular tissue, compared to G3. CONCLUSIONS Renal fibrosis is potentially reversible and therapeutic use of USCs can reverse specifically targeted decrease of influx of inflammatory macrophages, interstitial fibrosis formation and collagen deposition through their paracrine effects. Implantation of USCs has potential in the prevention and treatment of renal insufficiency. © 2017FiguresReferencesRelatedDetails Volume 197Issue 4SApril 2017Page: e293 Advertisement Copyright & Permissions© 2017MetricsAuthor Information Ting Long More articles by this author Yaodong Jiang More articles by this author Hua Shi More articles by this author Liren Zhong More articles by this author Deying Zhang More articles by this author Wei Li More articles by this author Yong Zhang More articles by this author Dong Chen More articles by this author Yan Jiao More articles by this author Debra Diz More articles by this author Yuanyuan Zhang More articles by this author Expand All Advertisement Advertisement PDF downloadLoading ...