Tube Technique Research Articles

Guidelines for the management of tracheal intubation in critically ill adults

These guidelines describe a comprehensive strategy to optimize oxygenation, airway management, and tracheal intubation in critically ill patients, in all hospital locations. They are a direct response to the 4th National Audit Project of the Royal College of Anaesthetists and Difficult Airway Society, which highlighted deficient management of these extremely vulnerable patients leading to major complications and avoidable deaths. They are founded on robust evidence where available, supplemented by expert consensus opinion where it is not. These guidelines recognize that improved outcomes of emergency airway management require closer attention to human factors, rather than simply introduction of new devices or improved technical proficiency. They stress the role of the airway team, a shared mental model, planning, and communication throughout airway management. The primacy of oxygenation including pre- and peroxygenation is emphasized. A modified rapid sequence approach is recommended. Optimal management is presented in an algorithm that combines Plans B and C, incorporating elements of the Vortex approach. To avoid delays and task fixation, the importance of limiting procedural attempts, promptly recognizing failure, and transitioning to the next algorithm step are emphasized. The guidelines recommend early use of a videolaryngoscope, with a screen visible to all, and second generation supraglottic airways for airway rescue. Recommendations for emergency front of neck airway are for a scalpel–bougie–tube technique while acknowledging the value of other techniques performed by trained experts. As most critical care airway catastrophes occur after intubation, from dislodged or blocked tubes, essential methods to avoid these complications are also emphasized.

Viscosity of CO2-rich mixtures from 243 K to 423 K at pressures up to 155 MPa: New experimental viscosity data and modelling

Abstract The viscosities of three multi-component CO2-rich mixtures were measured using the classical capillary tube technique. The tests were conducted at pressures from 1 to 155 MPa at various temperatures from 243 to 423 K in the gas, liquid and supercritical regions. Correlative and predictive models were evaluated using the measured experimental viscosity data. The correlative model, i.e., Lohrenz-Bray-Clark (LBC) model, was tuned to match the experimental data (CO2-LBC after tuning) and predictive models were modified by replacing the reference fluids. The predictive models in this work are based on the corresponding states (CS) theory models. The one reference fluid corresponding states model is the model originally developed by Pedersen which was modified by changing the reference fluid from methane to CO2 (called hereafter to CO2-Pedersen); two reference fluid corresponding states model is the Aasberg-Petersen (CS2) approach which was modified by selecting CO2 as reference fluid for CO2-rich fluids instead of n-decane which is suitable for hydrocarbons (CO2-CS2 model). The SUPERTRAPP predictive model, based on the extended corresponding states (ECS) theory, was also modified by changing the reference fluid propane to carbon dioxide (CO2-SUPERTRAPP). Comparisons for the investigated mixtures show that the overall Absolute Average Deviation (AAD) for the CO2-Pedersen, CO2-SUPERTRAPP, CO2-CS2 and CO2-LBC are 3.8%, 4.6%, 5.3% and 9.2%, respectively. Overall, the CO2-Pedesen viscosity model is the most accurate model when predicting the viscosity of the investigated CO2-rich mixtures. As the viscosity is a function of density, the effect of the mixture density on the mixture viscosity was investigated. Also, the viscosity reduction of pure CO2 due to the presence of impurities in each system was demonstrated.

Powder diffraction study of Pd2HgSe3

The Pd2HgSe3 phase was synthetized from individual elements by the silica glass tube technique and its crystal structure has been refined by the Rietveld method. The Pd2HgSe3 phase crystalizes in P$\bar 3$m1 space group with the unit-cell parameters a = 7.3096(2) Å, c = 5.2829(1) Å, V = 244.45(1) Å3, Dc = 8.84 g/cm3, and Z = 2. In its layered crystal structure, the [PdSe6] octahedra share opposing Se–Se edges with adjacent [PdSe4] squares forming layers parallel with the (001) plane. The layers show AA type stacking along the c-axis. Hg atoms occupy the anti-cubooctahedral voids between two consecutive layers. Pd2HgSe3 is isostructural with Pt2HgSe3 and Pt4Tl2X6 (X = S, Se, or Te) phases. The structure can be viewed as a 2a.2a.c superstructure of PtSe2.

Linseed oil and DGAT1 K232A polymorphism: Effects on methane emission, energy and nitrogen metabolism, lactation performance, ruminal fermentation, and rumen microbial composition of Holstein-Friesian cows

Complex interactions between rumen microbiota, cow genetics, and diet composition may exist. Therefore, the effect of linseed oil, DGAT1 K232A polymorphism (DGAT1), and the interaction between linseed oil and DGAT1 on CH4 and H2 emission, energy and N metabolism, lactation performance, ruminal fermentation, and rumen bacterial and archaeal composition was investigated. Twenty-four lactating Holstein-Friesian cows (i.e., 12 with DGAT1 KK genotype and 12 with DGAT1 AA genotype) were fed 2 diets in a crossover design: a control diet and a linseed oil diet (LSO) with a difference of 22 g/kg of dry matter (DM) in fat content between the 2 diets. Both diets consisted of 40% corn silage, 30% grass silage, and 30% concentrates (DM basis). Apparent digestibility, lactation performance, N and energy balance, and CH4 emission were measured in climate respiration chambers, and rumen fluid samples were collected using the oral stomach tube technique. No linseed oil by DGAT1 interactions were observed for digestibility, milk production and composition, energy and N balance, CH4 and H2 emissions, and rumen volatile fatty acid concentrations. The DGAT1 KK genotype was associated with a lower proportion of polyunsaturated fatty acids in milk fat, and with a higher milk fat and protein content, and proportion of saturated fatty acids in milk fat compared with the DGAT1 AA genotype, whereas the fat- and protein-corrected milk yield was unaffected by DGAT1. Also, DGAT1 did not affect nutrient digestibility, CH4 or H2 emission, ruminal fermentation or ruminal archaeal and bacterial concentrations. Rumen bacterial and archaeal composition was also unaffected in terms of the whole community, whereas at the genus level the relative abundances of some bacterial genera were found to be affected by DGAT1. The DGAT1 KK genotype was associated with a lower metabolizability (i.e., ratio of metabolizable to gross energy intake), and with a tendency for a lower milk N efficiency compared with the DGAT1 AA genotype. The LSO diet tended to decrease CH4 production (g/d) by 8%, and significantly decreased CH4 yield (g/kg of DM intake) by 6% and CH4 intensity (g/kg of fat- and protein-corrected milk) by 11%, but did not affect H2 emission. The LSO diet also decreased ruminal acetate molar proportion, the acetate to propionate ratio, and the archaea to bacteria ratio, whereas ruminal propionate molar proportion and milk N efficiency increased. Ruminal bacterial and archaeal composition tended to be affected by diet in terms of the whole community, with several bacterial genera found to be significantly affected by diet. These results indicate that DGAT1 does not affect enteric CH4 emission and production pathways, but that it does affect traits other than lactation characteristics, including metabolizability, N efficiency, and the relative abundance of Bifidobacterium. Additionally, linseed oil reduces CH4 emission independent of DGAT1 and affects the rumen microbiota and its fermentative activity.

Gene transfer utilizing pollen-tubes of Albuca nelsonii and Tulbaghia violacea

Abstract Developing a tissue culture-independent genetic transformation system would be an interesting technique for gene transfer in valuable medicinal and horticultural plants. Efficient gene delivery (Agrobacterium tumefaciens strain LBA 4404: harbouring PBI121 plasmid) was achieved with Km-resistant pollen grains (pollen tube technique) and were found to be GUS-positive for Albuca nelsonii (31.3%) and Tulbaghia violacea (32.6%). The Km-resistance (95.6% for A. nelsonii and 86.7% for T. violacea) and GUS-positive (100% for A. nelsonii and 97.5% for T. violacea) putative transgenic seedlings in vitro were obtained with 200 mg L-1 Km. The in vitro plants were obtained from leaf explants of putative transgenic seedlings and were confirmed to be Km-resistant and GUS-positive (T. violacea, 73.7% and A. nelsonii, 80.5%). The plants were successfully acclimatized in the greenhouse. We describe a tissue culture-independent gene transfer technique with high efficiency clonal transgenic plant production for A. nelsonii and T. violacea. This can also be applied to biotechnological crop improvement of the same species and potentially to other plants.

Residual stress and springback analysis for 304 stainless steel tubes in flexible-bending process

Flexible-bending is an innovative processing technique for profiles and tubes, which adjusts the curved shape of the forming part through numerical control. In this article, the flexible-bending forming precision of 304 stainless steel tubes was studied. The flexible-bending process of the tubes was simulated through finite element method in comparison with that of experiments. After the investigations of forming springback and residual stress, we conclude that regardless of changes in wall thickness or outer diameter, the residual stress decreases first and then increases following the increase of the ratio between outer diameter and wall thickness. Neither the wall thickening rate nor thinning rate is affected by wall thickness changes. The thrust of the pusher maintains stable during the bending process. The changes of both wall thickness and outer diameter affect the springback to some extents. When the radius of the target is constant, the springback can be optimized by adjusting the offset of the bending die and the distance between the guide and the die. The experiments agree well with the numerical simulations.

Poractant alfa in respiratory distress syndrome in preterm infants: a profile of its use

Poractant alfa (Curosurf®) is a natural, porcine-derived surfactant that is well established as an effective and generally well-tolerated agent in the treatment of respiratory distress syndrome (RDS) in preterm infants. The efficacy of poractant alfa in RDS is better than that of early-generation synthetic surfactants, and limited data are available regarding its efficacy versus new-generation synthetic agents. Poractant alfa 200 mg/kg is more effective than poractant alfa 100 mg/kg or bovine-derived beractant 100 mg/kg. Techniques for administering poractant alfa include via an endotracheal tube or less invasive techniques [e.g. less invasive surfactant administration (LISA)] performed during non-invasive ventilation of spontaneously breathing infants via nasal continuous positive airway pressure (CPAP). Administration of poractant alfa 200 mg/kg in early rescue [i.e. fraction of inspired oxygen (FiO2) ≥ 0.3] via LISA is associated with increased successful management on continuous positive airway pressure, as well as reductions in the incidence of bronchopulmonary dysplasia (BPD) and death/BPD.

Comparison of numerical analysis on the downhole flow field for multi-orifice hydrothermal jet drilling technology for geothermal wells

Newly developed hydrothermal jet drilling technology has the potential of being economically advantageous over conventional drilling techniques for drilling deep wells in hard formations. By applying coiled tubing techniques and modulating fluid media in the bottomhole reaction chamber, there can be a high temperature and high velocity jet striking and conducting heat to break the rock. So far, there has been no specific study on the influence of nozzle structure on the flow field of multiple hydrothermal jets. This paper presents hydrothermal jet models with different numbers of orifices to investigate the features of flow field, carrying capacity, drilling ability and cooling effect. Results show that for two models in the absence of cooling water, the bottomhole center temperature and pressure are higher than the two sides under multiple hydrothermal jets conditions. This is similar to the flow pattern for a single jet. Additionally, for the five-orifice nozzle with cooling water injected, the entire high temperature region is cylindrical. Ambient cooling water envelops the inner hot water. By comparing different models, the five-orifice nozzle model without cooling water shows a circular symmetric distribution of the bottomhole temperature. With cooling water injected, the central high temperature region becomes rectangular, while the margin of the well bottom is cooled by the peripheral cooling water. The bottom rock average temperature in five-orifice model is lower than for the four-orifice model due to more drastic thermal and kinetic transfer between the hydrothermal jet and the cooling water. The five-orifice nozzle model is better than the four-orifice nozzle model in terms of bottomhole temperature, bottomhole pressure and carrying capacity. Therefore, the five-orifice nozzle should be adopted for hydrothermal jet drilling. It is also feasible to pump down relatively high temperature cooling water to guarantee the high temperature downhole environment. Meanwhile, the cooling water pressure should be controlled during the drilling process for better cooling efficiency. All results in this paper are relevant to the parameters design for multi-orifice hydrothermal jet drilling technology.

Outcome After Subarachnoid Hemorrhage from Blood Blister-Like Aneurysm Rupture Depends on Age and Aneurysm Morphology.

Blood blister-like aneurysms (BBLA) are rare, challenging to treat, and prone to rerupture. We analyzed our results in relationship to type of BBLA and other prognostic factors. Data on patient and aneurysm characteristics, clinical course, and treatment results, including data from patient records and review of imaging findings, were collected prospectively and analyzed retrospectively. There were 27 patients (mean age 56 years) with subarachnoid hemorrhage from BBLA. Of patients, 13 (48%) had BBLA of the internal carotid artery, 6 had BBLA of the anterior cerebral artery (22%), 5 had BBLA of the middle cerebral artery (19%), and 3 had BBLA of the basilar artery (11%). Most patients (n= 12) had a circumferential type IV BBLA (44%). BBLAs were treated by clip-reinforced wrapping in 13 patients (48%) and by clipping alone in 8 patients (30%); 3 (11%) patients received no treatment. The 3 remaining patients were treated endovascularly, by combined treatment, or by fenestration tube technique. Rerupture after treatment occurred in 4 patients (15%), all of whom had type IV BBLAs. Outcome was unfavorable (modified Rankin scale score 3-6) in 52% (n= 14). The highest rates of rerupture (33%), delayed infarctions (67%), unfavorable outcome (75%), and death (50%) were identified in type IV BBLAs; type I-III BBLAs had a better course. Treatment of BBLA is challenging. Patients with type IV BBLAs were especially prone to rerupture, delayed infarctions, unfavorable outcomes, and death. Independent risk factors for unfavorable outcome were advanced age (≥60 years) and type IV BBLA.

Screening for DEL phenotype in RhD negative Indians.

DEL phenotype represents a very weak form of D variant detected only by adsorption and elution technique. DEL phenotype individuals mistyped as RhD-negative can lead to alloimmunization after transfusion or pregnancy. Molecular techniques have now been used to identify DEL variants. They are commonly encountered in the East Asian population with RHD(K409K) being the most frequent allele. RHD(M295I) is the most common DEL allele in Caucasians. As there is a paucity of data on DEL phenotype in the Indian population, the study aims to screen RhD negative individuals for two most common DEL mutations. EDTA blood was collected from 900 RhD negative individuals. Serological analysis included testing for the five major Rh antigens- C, c, D, E, and e by tube technique. Samples showing negative reaction for the presence of D antigen by Indirect Antiglobulin test were further tested for DEL phenotype by adsorption and elution technique. Molecular analysis involved DNA extraction and testing by PCR-SSP for RHD(K409K) and RHD(M295I) DEL alleles. Rh phenotyping showed 153 Rh negative individuals with r'r, ten with r''r and 737 with rr phenotype. All the samples tested negative for RhD antigen by adsorption and elution method. The two common DEL mutations RHD(K409K) and RHD(M295I) were also not detected in the study population. The study population showed the absence of the two common DEL alleles, concluding the variant to be rare. A comprehensive study with a larger sample size to look for other DEL mutations should be performed.

Kinetic Studies on the Reactions of Atomic Oxygen with Furan, 2-Methylfuran, and 2,5-Dimethylfuran at Elevated Temperatures

Abstract The reactions of O(3P) atoms with furan, 2-methylfuran (2-MF), and 2,5-dimethylfuran (2,5-DMF) were studied at elevated temperatures by using a shock tube technique coupled with atomic resonance absorption spectroscopy (ARAS). The rate coefficients (k) determined from the time profiles of O(3P) atoms were expressed by the relation of kO+2,5-DMF > kO+2-MF > kO+FURAN. The molecular orbital calculations with CBS-QB3 level were also performed and the possible product channels of these reactions were discussed.

Thiomers: Impact of in situ cross-linkers on mucoadhesive properties

The aim of this study was to evaluate the impact of in situ cross-linkers on the gelling and mucoadhesive properties of thiomers. Polycarbophil-cysteine conjugate (PCP-cys) was synthesized by covalent attachment of l-cysteine to polycarbophil via amide bond formation mediated by 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide hydrochloride (EDAC) and N-hydroxysuccinimide (NHS) whereas in situ cross-linkers (PAA-cys-MNA) were synthesized by the same bond formation between poly(acrylic acid) (PAA) of 2.1-, 6-, and 15kDa and 2-((2-amino-2-carboxyethyl)disulfanyl)nicotinic acid (cys-MNA) used as ligand. The in situ cross-linking properties were studied via rheological measurements of dynamic viscosity of mixtures of PCP-cys and PAA-cys-MNA with purified porcine intestinal mucus and via rotating cylinder method. The diffusion of polymers in purified porcine intestinal mucus was studied via rotating tube technique. The results showed that in situ cross-linkers (PAA 2.1-, 6-, 15kDa) increase the dynamic viscosity of PCP-cys/mucus mixtures by 5.1-, 5.6-, and 3.5-fold. Combinations of 10% of in situ cross-linkers PAA 2.1-, 6- or 15kDa and 90% PCP-cys increased the adhesion time 1.1-, 2.0- and 4.9-fold, respectively, compared to PCP-cys alone. Diffusion study showed that low molecular mass PAAs highly penetrate into the mucus gel layer due to their high polymer chain mobility compared to PCP-cys. The results provide evidence for the potential of in situ cross-linking agents as gelling and mucoadhesion enhancers.

Bacteriological profile of water samples in and around Shimla hills: a study from the sub Himalayan region

Background: The present study was done to assess the bacteriological quality of drinking water in and around Shimla in the event of an outbreak of hepatitis E in Shimla city, January 2016. Methods: Total 1098 water samples from different sources were received from January to July 2016. The bacteriological analysis of water was done by the multiple tube technique. Results were interpreted after 48 hours of incubation of the water sample in MacConkey bile broth medium in accordance with Mc Crady probability table. Results: A total of 1098 water samples were received in the Microbiology laboratory of which 129 (11.74%) were unsatisfactory, 25 (2.27%) satisfactory and rest 925 (84.24%) were excellent. Nearly 9.21% samples from water tanks, 2.3% from public taps were unsatisfactory but water samples from all the water ATM s were excellent. Escherichia coli were the commonest isolate 35.6%, Klebsiella pneumoniae 31.6%, Klebsiella oxytoca 19.3%, Enterobacter sps 8% and Citrobacter sps 2%. In the corresponding period 477 patients had come to the health facilities for clinical symptoms of jaundice. The serum samples from clinically suspected cases were subject to antibody testing for IgM HAV and IgM HEV and it revealed that 109 (23%) were positive for HAV while 253 (53%) were positive for HEV. During the above period 75 (15%) patients had co-infection with both HAV and HEV. Conclusions: Bacteriological assessment of drinking water is essential and should be carried out on regular basis so as to prevent outbreaks of Hepatitis A and E and other water borne diseases.

Experimental investigations and phase-field simulations of triple-phase-separation kinetics within liquid ternary Co-Cu-Pb immiscible alloys.

The phase-separation kinetics and microstructure evolution mechanisms of liquid ternary Co_{43}Cu_{40}Pb_{17} immiscible alloys are investigated by both the drop tube technique and phase-field method. Two successive phase separations take place during droplet falling and lead to the formation of a three-phase three-layer core-shell structure composed of a Co-rich core, a Cu-rich middle layer, and a Pb-rich shell. The Pb-rich shell becomes more and more conspicuous as droplet diameter decreases. Meanwhile, the Co-rich core center gradually moves away from the core-shell center. Theoretical analyses show that a larger temperature gradient inside a smaller alloy droplet induces the accelerated growth of the surface segregation shell during triple-phase separation. The residual Stokes motion and the asymmetric Marangoni convection result in the appearance of an eccentric Co-rich core and the core deviation degree is closely related to the droplet size and initial velocity. A three-dimensional phase-field model of ternary immiscible alloys, which considers the successive phase separations under the combined effects of Marangoni convection and surface segregation, is proposed to explore the formation mechanisms of three-phase core-shell structures. The simulated core-shell morphologies are consistent with the experimental observations, which verifies the model's validity in reproducing the core-shell dynamic evolution. Numerical results reveal that the development of three-phase three-layer core-shell structures can be attributed to the primary and then secondary phase separations dominated simultaneously by Marangoni convection and surface segregation. Furthermore, the effects of droplet temperature gradient on the growth kinetics of the surface segregation shell are analyzed in the light of phase-field theory.

Ocurrencia de Enteroparásitos en Poblaciones de Quiscalus lugubris (Aves: Passeriformes, Icteridae) del Semiárido Urbano del Estado Falcón, Venezuela

Los parásitos intestinales patógenos de aves paseriformes pueden potencialmente tener importancia zoonótica. Se realizó un estudio para determinar la ocurrencia de parásitos intestinales en heces de poblaciones de Quiscalus lugubris («torditos negros») (Aves, Passeriformes: Icteridae) en la ciudad de Coro, estado Falcón, Venezuela. El diagnóstico parasitológico de las muestras (n=156) se hizo mediante los métodos directo, flotación de Willis-Molloy y Faust, sedimentación en tubo y coloración de Kinyoun. Se detectaron hasta tres taxones de protozoarios, helmintos y acantocéfalos en 109 muestras (69.9%). Isospora spp (59.0%), Criptosporidium spp (37.8%) y el acantocéfalo Mediorhynchus spp (6.4%) fueron los enteroparásitos más frecuentes. Todos los parásitos encontrados representan nuevos registros para Q. lugubris en Venezuela.

Measuring the “glottal waveform” in laringectomized patients

There are several noninvasive and well-devised techniques for measuring the glottal waveform of human subjects, most of which rely on source-filter separation by digital inverse filtering. A more direct and simple approach relies on the use of the Sondhi tube technique to record the subjects’ voice, from which the glottal waveform is extracted, assuming that the effect of the vocal tract is negligible on the recorded sound. Although these techniques can capture the characteristics of the source waveform from normal speakers with reasonable accuracy, they have limitations when used to capture the source waveform of tracheoesophageal and esophageal speakers. This is due to the fact that these types of speech are highly aperiodic, which may compromise the accuracy of inverse filtering techniques. Moreover, the acoustic coupling between the vocal tract and the source in tracheoesophageal and esophageal speech is not well understood yet, and the assumptions implied by the Sondhi tube techniques cannot be taken. This work proposes a new experimental technique to capture the source waveform of tracheoesophageal and esophageal speakers based on the layer-peeling technique using a open-closed tube with two microphones and a sound source. Results for the source waveform of both normal and tracheoesophageal speakers are captured with the new technique and compared with traditional methods based on both inverse filtering and the Sondhi tube.

The gas-phase methylation of benzene and toluene

The reactions of the methyl cation with benzene and toluene in the gas phase have been examined using the flowing afterglow-selected ion flow-drift tube technique. With benzene four product ions are formed, C6H6+ by electron transfer, C6H5+ by addition and loss of CH4, C7H7+ by addition and loss of H2, and an adduct C7H9+. Deuterium and carbon-13 labeling experiments were carried out to provide mechanistic insights. In agreement with earlier work, deuterium labeling (CD3+ with C6H6 or CH3+ with C6D6) shows that partial H/D scrambling between the methyl group and the ring occurs during the formation of C6H5+ and C7H7+. However, in contrast to earlier work, no carbon-13 scrambling was observed between the methyl and ring carbons, thus ruling out a ring expansion and contraction mechanism to account for the H/D scrambling. Nor did we find H/D scrambling in the electron transfer product ion, C6H6+. When collision-induced dissociation (CID) was carried out on the adduct ion, extensive H/D and carbon-13 scrambling was found, indicating that at least some ring expansion occurs during its formation. Reaction of C6H5+ with methane at room temperature exclusively forms the adduct ion; in a drift field, this adduct ion fragments by loss of CH4 and H2. Mechanisms are proposed which account for our results, and these are supported by ab initio calculations. Similar studies were carried out with toluene as the neutral reagent. Besides the four analogous product ions, we found hydride transfer from the methyl group of toluene to be a major reaction channel and addition with loss of ethylene to be a minor channel.

Comparison of micro column technology with conventional tube methods for antibody detection.

BACKGROUND AND OBJECTIVES:Conventional tube technique (CTT) has been the mainstay for antibody detection in pretransfusion testing. There have been rapid technological advances in blood banking and methodology of crossmatch has been modified to improve the sensitivity of these tests and to enable automation. This study was done to compare the efficacy of three crossmatch techniques: CTT, tube low-ionic-strength-saline indirect antiglobulin test (tube LISS-IAT), and micro column technology (MCT) used in the blood bank serology laboratory.MATERIALS AND METHODS:In this prospective study, 150 samples from patients who had received two or more transfusions on two different occasions (with at least 72 h between two transfusions) were subjected to cross match by three different techniques – CTT, LISS-IAT, and MCT.RESULTS:A total of 16 cases with antibodies were identified in 150 patients. Out of 16 cases, 14 were clinically significant (anti-c = 5, anti-K = 4, anti-E = 2, anti-S = 2, anti-Jka = 1) and 2 nonclinically significant antibody cases (anti-Lea). MCT detected all the 14 clinically significant antibody cases and no case of nonclinically significant antibody. Tube LISS-IAT detected 14 antibody cases including 2 cases of non-clinically significant antibody but failed to detect 1 case of anti-c and the only case of anti-Jka. CTT detected only 10 antibody cases including 2 cases of non-clinically significant antibody and but failed to detect 3 cases of anti-c, 1 case of anti-K, 1 case of anti-E, and the only case of anti-Jka.CONCLUSION:MCT was found to be most efficacious when compared to CTT and tube LISS-IAT in detecting clinically significant red cell antibodies; although MCT missed 2 cases of Lea antibody which were detected by CTT and LISS-IAT.

V20 - Long term follow up and outcome of a new technique of ureteroileal anastomosis: Tube in tube technique

V20 - Long term follow up and outcome of a new technique of ureteroileal anastomosis: Tube in tube technique

Diagnóstico espacial e temporal de condições físico-químicas e microbiológicas do Córrego do Tanquinho, Ribeirão Preto, SP, Brasil

O objetivo do estudo foi avaliar as condições fisico-químicas e microbiológicas do Córrego do Tanquinho, localizado em área altamente urbanizada e industrializada no município de Ribeirão Preto, SP, Brasil, considerando variações espaciais e temporais. Os parâmetros limnológicos avaliados foram: pH, temperatura, turbidez, condutividade e oxigênio dissolvido (OD), com medições realizadas in situ. Os níveis de metais no sedimento e em água superficial foram analisados por Espectrofotometria de Absorção Atômica. Para a determinação dos Coliformes Totais e Escherichia coli foi empregada a técnica dos Tubos Múltiplos com utilização de substrato cromogênico. Os parâmetros OD, E. coli e a concentração dos metais Cu e Mn na água encontram-se em desacordo com a legislação. Os metais encontrados em maior concentração no sedimento foram Mn, Cr e Zn. A avaliação microbiológica evidenciou o despejo de esgoto doméstico no córrego e as concentrações de metais indicam a possível ocorrência de efluentes industriais, podendo gerar graves problemas de saúde pública. O diagnóstico espacial indicou os locais de amostragem com as piores condições microbiológicas e altas concentrações de metais, enquanto que a influência temporal foi observada no pH, Mn e Cr em água superficial, e Mn, Cr e Pb nos sedimentos. Os resultados mostraram que o Córrego do Tanquinho é um corpo hídrico impactado. Recomenda-se a realização de outros estudos que possam esclarecer melhor a origem das cargas poluidoras, além da conscientização da população e da adoção de medidas que possibilitem a recuperação e proteção desse corpo hídrico.