Event Abstract Back to Event Molecular and biometric characterization of natural bream × roach hybrids population in the Dobczyce Reservoir (S Poland) Maciej K. Konopiński1* and Antoni Amirowicz1 1 Institute of Nature Conservation, Polish Academy of Sciences, Poland Natural hybrids of leuciscine cyprinids were investigated in a medium-sized submontane reservoir (49°52'N, 20°03'E, altitude 270 m) in the Carpathian part of the Vistula basin. The material included 380 hybrid specimens (TL: 24.0–39.8 cm, SL: 19.0–31.7 cm, W: 134–714 g) collected in 2006–2013. To detect their ancestry, genotypes of the 327 putative hybrids were compared to reference genotypes of 85 roaches, 115 common breams, 18 rudds, and 99 silver breams. Individuals were typed in 15 polymorphic microsatellite loci (Dubut et al. 2009), while species specific amplification of a fragment of mitochondrial cytochrome b gene was used to detect maternal origin of hybrids (Wyatt et al. 2006). Few individuals with traces of rudd and silver bream ancestry were excluded from the study. Analysis of mitochondrial DNA suggests bias towards bream maternal origin, with only 22 individuals (6,7%) with roach mitochondrial DNA. The analyzed set of hybrids was composed mostly of first generation bream × roach hybrids. Only 7 individuals (2,1%) showed a sign of backcrossing to bream, however, this finding has to be confirmed by analysis of further loci as it might result from presence of null alleles in roach. F2 generation hybrids were not detected. Biometric investigation of collected specimens comprised of selected morphometric (body height, and lengths of head, trunk, and tail) and meristic characters (counts of scales in lateral line and soft rays in anal fin) of individuals of different maternal origin. As all the analyzed differences between these categories of specimens appeared insignificant, the investigated population of hybrids may be regarded morphologically uniform. Figure 1 References Dubut, V., Martin, J.F., Gilles, A., Van Houdt, J., Chappaz, R., Costedoat, C. (2009). Isolation and characterization of polymorphic microsatellite loci for the dace complex: Leuciscus leuciscus (Teleostei: Cyprinidae). Mol. Ecol. Resour. 9(4): 1179-1183. doi: 10.1111/j.1755-0998.2009.02594.x. Wyatt, P.M.W. , Pitts, C.S., Butlin, R.K. (2007). A molecular approach to detect hybridization between bream Abramis brama, roach Rutlius rutilus and rudd Scardinius erythrophthalmus. J. Fish Biol. 69(sa):52 - 71. doi: 10.1111/j.1095-8649.2006.01104.x. Keywords: Dam reservoirs, Hybridization, Genetic, Roach, Common bream, morphometry, microsatellites Conference: XV European Congress of Ichthyology, Porto, Portugal, 7 Sep - 11 Sep, 2015. Presentation Type: Poster Presentation Topic: Phylogeny, Systematics and Genetics Citation: Konopiński MK and Amirowicz A (2015). Molecular and biometric characterization of natural bream × roach hybrids population in the Dobczyce Reservoir (S Poland). Front. Mar. Sci. Conference Abstract: XV European Congress of Ichthyology. doi: 10.3389/conf.fmars.2015.03.00112 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 20 Nov 2015; Published Online: 22 Nov 2015. * Correspondence: Dr. Maciej K Konopiński, Institute of Nature Conservation, Polish Academy of Sciences, Kraków, 31-120, Poland, konopinski@iop.krakow.pl Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Maciej K Konopiński Antoni Amirowicz Google Maciej K Konopiński Antoni Amirowicz Google Scholar Maciej K Konopiński Antoni Amirowicz PubMed Maciej K Konopiński Antoni Amirowicz Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.