The purpose of this study was to compare the effects of myofascial release and muscle energy on acute outcomes in trunk extensors active range-of-motion and strength in recreationally resistance-trained women. Seventeen apparently healthy women performed three experimental protocols using a cross-over, randomized (counterbalanced in Latin Square format), and within-subjects design: a) range-of-motion and strength test after a manual myofascial release protocol (MFR); b) flexibility and strength test after a muscle energy protocol (ME); and c) range-of-motion and strength test without myofascial release or muscle energy (control condition). Active trunk range-of-motion was measured via a sit-and-reach test and trunk extension strength via isometric dorsal dynamometer. A significant increase in range-of-motion was found for MFR (p = 0.002; d = 0.71) and ME (p < 0.001; d = 0.47) when comparing post-intervention with baseline values. Similarly, a significant increase for strength was found for MFR (p = 0.018; d = 0.10) when comparing post-intervention with baseline values. In conclusion, both techniques (MFR and ME) improved trunk range-of-motion with the sit and reach test immediately post-intervention; however, MFR showed greater magnitude increases in range-of-motion (MFR: (medium magnitude) vs ME: small magnitude). Due to the potential health implications, both (MFR and ME) responses should be among the many considerations for rehabilitation and performance exercise prescription when prescribing an exercise regimen.