True Protein Content Research Articles

Comparing electrostatic separation of soy and lupin: Effect of de-oiling by solvent extraction

Electrostatic separation is a sustainable dry separation technique based on triboelectric charging of different cellular tissue components (i.e. protein bodies and fibres). This research aimed to determine the mechanism behind the ineffective electrostatic separation of soy and if it could be altered by de-oiling. Several scenarios were compared, which involved electrostatic separation of soy and lupin de-oiled with different solvents (none, acetone, ethanol and hexane). Separation of lupin resulted in a higher true protein content (58.5%DM (dry matter) (Nfactor = 5.7)) than separation of soy (45.0%DM protein). Separation was less effective for soy because its protein bodies were still embedded in the cellular structure after impact milling, which was also reflected in the larger particle size and lower small particle dispersibility. De-oiling soy with hexane and extra milling improved the purity of soy protein-enriched fractions (59.6%DM protein) reaching more similar purity as for lupin protein-enriched fractions. The protein purity of lupin fractions could be increased most via the use of polar solvents (acetone and ethanol). Better electrostatic separation after de-oiling and extra milling for soy could be explained by increased liberated protein bodies, which was key towards the improved separation of soy obtaining protein purities closer to that of pure protein bodies.

Lactational performance effects of supplemental histidine in dairy cows: A meta-analysis

The objective of this meta-analysis was to examine the effects of supplemental His on lactational performance, plasma His concentration and efficiency of utilization of digestible His (EffHis) in dairy cows. The meta-analysis was performed on data from 17 studies published in peer-reviewed journals between 1999 and 2022. Five publications reported data from 2 separate experiments, which were included in the analyses as separate studies, therefore resulting in a total of 22 studies. In 10 studies, His was supplemented as rumen-protected (RP) His; in 1 study, 2 basal diets with different dHis levels were fed; and in the remaining experiments, free His was infused into the abomasum (4 studies), the jugular vein (3 studies) or deleted from a mixture of postruminally infused AA (4 studies). The main forages in the diets were corn silage in 14 and grass silage in 8 studies. If not reported in the publications, the supplies of dietary CP, metabolizable protein (MP), net energy of lactation, and digestible His (dHis) were estimated using NRC (2001). An initial meta-analysis was performed to test the standard mean difference (SMD; raw mean difference of treatment and control means divided by the pooled standard deviation of the means), that is, effect size, and the corresponding 95% confidence interval (CI) in production parameters between His-supplemented groups versus control. Further, regression analyses were also conducted to examine and compare the relationships between several response variables and dHis supply. Across studies, His supplementation increased plasma His concentration (SMD = 1.39; 95% CI: 1.17-1.61), as well as DMI (SMD = 0.240; 95% CI: 0.051-0.429) and milk yield (MY; SMD = 0.667; 95% CI: 0.468-0.866), respectively. Further, milk true protein concentration (MTP; SMD = 0.236; 95% CI: 0.046-0.425) and milk true protein yield (MTPY; SMD = 0.581; 95% CI: 0.387-0.776) were increased by His supplementation. Notably, the increase in MTP concentration and MTPY were 3.9 and 1.3 times greater for studies with MP-deficient (according to NRC 2001) diets compared with studies with MP-adequate diets. The regression analyses revealed that production parameters (DMI, MY, and MTPY) responded in a nonlinear manner to increasing His supply. Further, we detected a difference in the magnitude of change in MTPY and plasma His concentration with the level of His supply and between His supplementation methods, being greater for infused His compared with RPHis. Lastly, a linear and negative relationship between EffHis and the ratio of total digestible His to net energy for lactation supply was observed, indicating an important interaction between dHis and energy supply and EffHis (i.e., utilization of dHis to support protein export). Overall, these analyses confirm His as an important AA in dairy cattle nutrition.

Effects of Phenyllactic Acid on Fermentation Parameters, Nitrogen Fractions and Bacterial Community of High-Moisture Stylo Silage

To investigate the effect of phenyllactic acid (PLA) on the dynamic changes of high-moisture stylo silage, fresh stylo was ensiled with addition of PLA at the levels of 0, 1% and 2% using lab-level silage bags, where samples were collected on days 3, 7, 14 and 30 of ensiling fermentation to analyze fermentation parameters, nitrogen distribution and bacterial community. The results showed that PLA addition at ensiling led to the increase (p < 0.01) in dry matter content, lactic acid concentration and Flieg’s score of stylo silage as well as the decrease (p < 0.01) in dry matter loss, pH value and coliform bacteria population, with butyric acid only detected in the control group. It also resulted in the increase (p < 0.01) in true protein content and its proportion as well as the decrease (p < 0.01) in ammonia-N content and its proportion, almost with linearly dose effect. Sequencing analysis revealed that PLA addition led to the increase (p < 0.05) in Sobs, Shannon, Chao and Ace of bacterial community in the stylo silage, where the relative abundance of Enterobacter, Clostridium, and Kosakonia was decreased, and that of Lactobacillus, Enterococcus and Pantoea was increased. Furthermore, Kosakonia, Terriglobus, Sphingomonas and Sphingopyxis had an important role in the bacterial interaction network. It is suggested that PLA application at the level of 1–2% could improve silage quality of stylo silage via modifying bacterial community.

Dietary supplementation with xylooligosaccharides and exogenous enzyme improves milk production, energy utilization efficiency and reduces enteric methane emissions of Jersey cows

BackgroundSustainable strategies for enteric methane (CH4) mitigation of dairy cows have been extensively explored to improve production performance and alleviate environmental pressure. The present study aimed to investigate the effects of dietary xylooligosaccharides (XOS) and exogenous enzyme (EXE) supplementation on milk production, nutrient digestibility, enteric CH4 emissions, energy utilization efficiency of lactating Jersey dairy cows. Forty-eight lactating cows were randomly assigned to one of 4 treatments: (1) control diet (CON), (2) CON with 25 g/d XOS (XOS), (3) CON with 15 g/d EXE (EXE), and (4) CON with 25 g/d XOS and 15 g/d EXE (XOS + EXE). The 60-d experimental period consisted of a 14-d adaptation period and a 46-d sampling period. The enteric CO2 and CH4 emissions and O2 consumption were measured using two GreenFeed units, which were further used to determine the energy utilization efficiency of cows.ResultsCompared with CON, cows fed XOS, EXE or XOS + EXE significantly (P < 0.05) increased milk yield, true protein and fat concentration, and energy-corrected milk yield (ECM)/DM intake, which could be reflected by the significant improvement (P < 0.05) of dietary NDF and ADF digestibility. The results showed that dietary supplementation of XOS, EXE or XOS + EXE significantly (P < 0.05) reduced CH4 emission, CH4/milk yield, and CH4/ECM. Furthermore, cows fed XOS demonstrated highest (P < 0.05) metabolizable energy intake, milk energy output but lowest (P < 0.05) of CH4 energy output and CH4 energy output as a proportion of gross energy intake compared with the remaining treatments.ConclusionsDietary supplementary of XOS, EXE or combination of XOS and EXE contributed to the improvement of lactation performance, nutrient digestibility, and energy utilization efficiency, as well as reduction of enteric CH4 emissions of lactating Jersey cows. This promising mitigation method may need further research to validate its long-term effect and mode of action for dairy cows.

Safety of UV-treated powder of whole yellow mealworm (Tenebrio molitor larva) as a novel food pursuant to Regulation (EU) 2015/2283.

Following a request from the European Commission, the EFSA Panel on Nutrition, Novel Foods and Food Allergens (NDA) was asked to deliver an opinion on UV-treated powder of whole yellow mealworm (Tenebrio molitor larva) as a novel food (NF) pursuant to Regulation (EU) 2015/2283. The term yellow mealworm refers to the larval form of the insect species T. molitor. The NF is the UV-treated powder of the whole, thermally dried yellow mealworm. The NF consists mainly of crude protein, fat, digestible carbohydrates and fibre (chitin). The Panel notes that the levels of contaminants in the NF highly depend on the occurrence levels of these substances in the insect feed. The Panel notes furthermore that there are no safety concerns regarding the stability of the NF if the NF complies with the proposed specification limits during its entire shelf life. The NF has a high protein content, although the true protein content in the NF is overestimated when using the nitrogen-to-protein conversion factor of 6.25, due to the presence of non-protein nitrogen. The applicant proposed to use the NF as an ingredient in various food products, such as bakery products, pasta, compotes of fruit/vegetables and cheese. The target population is the general population. The Panel notes that considering the composition of the NF, the proposed conditions of use and that the NF will not be the sole source of dietary protein, the consumption of the NF is not nutritionally disadvantageous. Despite the UV treatment, the Panel notes that the NF is not a significant dietary contributor of vitamin D3. The submitted toxicity studies from the literature did not raise safety concerns. The Panel considers that the consumption of the NF may induce primary sensitisation and allergic reactions to yellow mealworm proteins and may cause allergic reactions in subjects with allergies to crustaceans and dust mites. Additionally, allergens from the feed may end up in the NF. With the exception of possible allergenicity, the Panel concludes that the NF is safe under the proposed uses and use levels.

The Effect of Solid-State Fermentation on the Nutritive Value of Rapeseed Cakes and Performance of Broiler Chickens

This study aimed to assess the applicability of eleven different products of solid-state fermentation of rapeseed cakes with commercial enzyme additives. Ground rapeseed cakes were mixed with water (1:2) and the enzymatic preparation (0.1%) according to the different variants and fermented at 25 °C for 24 h under anaerobic conditions. For fermentation, different enzymes were used: (1) α-amylase, (2) endo 1,4-β-xylanase, (3) endo-1,3(4)β-glucanase, (4) endo 1,4-β-xylanase, endo-1,3(4)β-glucanase, (5) α-amylase, endo-1,3(4)β-glucanase, (6) 6-phytase, (7) RONOZYME HiPhos 6-phytase, (8) liquid exogenous-6-phytase; and mixed combinations: (9) endo 1,4-β-xylanase, endo-1,3(4)β-glucanase, liquid exogenous-6-phytase, (10) α-amylase, endo-1,3(4)β-glucanase, liquid exogenous-6-phytase, and (11) α-amylase, endo 1,4-β-xylanase, endo-1,3(4)β-glucanase, liquid exogenous-6-phytase. After fermentation, the crude protein content in the products was similar, but the true protein content was significantly higher than in nonfermented rapeseed cakes (p &lt; 0.05). Fermentation significantly reduced (p &lt; 0.05) the levels of phytate phosphorous, raffinose family oligosaccharides, and glucosinolates in the products. In the next step, the most advantageous fermentation product obtained using liquid exogenous-6-phytase was selected in terms of nutritional value, produced on the technical scale, and incorporated into the diets for broilers. Six diets containing 12, 17, or 22% raw or fermented rapeseed cakes, respectively, were prepared. Up to 22% fermented rapeseed cakes did not adversely affect broiler production rates. Replacing raw rapeseed cakes with fermented products improved body weight gain in the grower phase, lowered feed intake in the finisher phase, and lowered the feed conversion ratio in all periods. A level of 22% of naturally fermented rapeseed cakes with liquid exogenous-6-phytase in the diet of broilers could be recommended.

Effects of Ammonification–Steam Explosion Pretreatment on the Production of True Protein from Rice Straw during Solid-State Fermentation

It is difficult to obtain high-protein contents from rice straw using direct fermentation due to its low nitrogen content. This study investigates the effects of ammonification–steam explosion pretreatment of rice straw on the protein content after solid-state fermentation (SSF). The pretreatment is carried out under multi-strain inoculation conditions. The samples of rice straw after ammonification (TA), steam explosion (TSE), and ammonification and steam explosion (TA-SE) were compared to the control group (TC). The results indicate that both ammonification and steam explosion could disintegrate rice straw’s lignocellulosic structure, releasing nutrients that can be used for microbial reproduction. In addition, amino compounds are formed along with depolymerization products, thus effectively promoting the true protein content. Post-fermentation, total crude protein contents of TA, TSE, and TA-SE samples were 2.56, 1.83, and 4.37 times higher than that of Tc samples, respectively, and true protein contents were 2.52, 1.83, and 5.03 times higher. This study shows that the true protein content by combined ammonification and steam explosion pretreatment of rice straw during 96 h of solid-state fermentation was 46.7% of its total matter, rendering it a suitable alternative to high-protein animal feed.

Effect of lactobacilli inoculation on protein and carbohydrate fractions, ensiling characteristics and bacterial community of alfalfa silage.

Alfalfa (Medicago sativa L.) silage is one of the major forages with high protein for ruminants. The objective of this study was to investigate the effects of lactobacilli inoculants on protein and carbohydrate fractions, ensiling characteristics and bacterial community of alfalfa silage. Wilted alfalfa (35% dry matter) was inoculated without (control) or with Lactobacillus coryniformis, Lactobacillus casei, Lactobacillus plantarum, and Lactobacillus pentosus and ensiled for 7, 15, and 60 days. Silage inoculated with L. pentosus was superior to L. coryniformis, L. casei, L. plantarum in improving the fermentation quality of alfalfa silage, as indicated by the lowest ammonia nitrogen content and silage pH during ensiling. There was minor difference in water soluble carbohydrates content among all silages, but L. pentosus inoculants was more efficient at using xylose to produce lactic acid, with lower xylose content and higher lactic acid content than the other inoculants. Compared with the control, L. pentosus inoculants did not affect true protein content of silage, but increased the proportions of buffer soluble protein and acid detergent soluble protein. The L. pentosus inoculants reduced the bacterial diversity In alfalfa silage with lower Shannon, Chao1, and Ace indices, and promoted relative abundance of lactobacillus and decreased the relative abundance of Pediococcus compared with the control. As well as L. pentosus inoculants up-regulated amino acid, carbohydrate, energy, terpenoids, and polypeptides metabolism, and promoted lactic acid fermentation process. In summary, the fermentation quality and nutrient preservation of alfalfa silage were efficiently improved by inoculated with L. pentosus.

Effect of substrate nutrient content level on nutritional expression of Hermetia illucens: a meta-analysis

This research aims to examine the effect of substrate nutrient content on nutrient expression of Hermetia illucens by using a meta-analysis method The database was constructed from studies that have reported various substrates with nutrient levels in Hermetia illucens. The nutritional content observed in this study were dry matter (DM), ash, crude protein (CP), true protein (TP) and ether extract (EE). There were 12 articles that met the criteria entered in the database. Mixed model methodology was used for statistical analysis. The difference in study data on Hermetia illucens was used as a random effect, and nutrient substrate level was used as a fixed effect. The statistical model used is the p-value. If the significance of an effect occurs, the p-value <0.05. The results showed that the increase in ash, CP, and TP on the substrate had a significant effect on the increase in nutrient Hermetia illucens (P<0.05) and increasing DM substrate tends to increased DM on Hermetia illucens (P<0.1). Increased EE levels in the substrate did not affect EE levels in Hermetia illucens larvae (P>0.05). The conclusion of this study was that the increase in ash and CP levels on the substrate had an effect on increasing the nutrient content of ash, CP, and TP of Hermetia illucens larvae.

Effect of Feeding Fermented Rice Bran by Rumen Liquor on the Growth Performance of Broiler Chickens

Background: Broiler feedstuff as Rice bran (RB) is a by-product of rice industry. Using RB in broiler chicken diets is not common, especially in broiler chicken production systems due to limitations like higher content of fiber and lower availability of micronutrients. Fermentation of RB by rumen inoculate would improve the nutritive value and further inclusion of urea as a non-protein nitrogenous substance would increase the microbial population using nitrogen generated from urea. Methods: RB was fermented anaerobically without or with urea (2.0%) by rumen inoculates for 48 hours. Three isonitrogenous (22.75% CP) and isocaloric (3164 kcal/kg) diets contained 7.0% RB, 7.0% fermented RB (FRB) and 7.0% urea (2.0%) added fermented RB (UFRB) formulated. The total number of 90-day old unsexed broiler chicks distributed in 03 groups considering 30 replicate birds in each. Result: True protein content increased in FRB (16.45%) and UFRB (16.85%) in comparison to RB group (16.27%). Final body weight of RB, FRB and UFRB groups 1129, 1152 and 1190 g/bird. Feed intake 1884, 1828 and 1924 g/bird respectively for RB, FRB and UFRB groups. Feed conversion ratio 1.75, 1.66 and 1.69 for RB, FRB and UFRB groups respectively. Dressing Percentage of Carcass yield is higher in UFRB (73%) than in FRB (70%). No mortality occurred in any groups during the experiment. So, it may be concluded that inclusion of fermented rice bran by rumen inoculate increase the performance of broiler but urea (2.0%) added to fermented rice bran by rumen inoculate shows better growth performance and carcass yield of broiler.

Postbiotic yeast fermentation product supplementation to lactating goats increases the efficiency of milk production by enhancing fiber digestibility and ruminal propionate, and reduces energy losses in methane.

Although in vitro data with mixed ruminal fluid demonstrated positive effects of posbiotic diet (POS) from lactobacilli on measures of fermentation and microbial profiles, there is a paucity of in vivo data with lactating ruminants. The aim of the study was to evaluate the effects of incorporating POS into diets of lactating goats on energy (E) partitioning, carbon (C) and nitrogen (N) balance, and performance. Ten late-lactation Murciano-Granadina goats were used in a crossover design with 26-d periods. Goats in the control diet (CON) were fed daily at the rate of 1 kg alfalfa hay and 1.5 kg concentrate, and the treatment group (POS) was fed CON with the addition of 3.75 g/d of Probisan Ruminants (PENTABIOL S.L., Navarra, Spain). No differences in DMI were detected. However, ruminal fluid propionate and apparent total tract digestibilities of NDF and ADF were greater (18%, 4.7%, and 5.2%, respectively; P < 0.05) in POS compared with the CON diet. Daily partitioning of E to milk and efficiency of ME intake for milk production greater (11% and 3.0%, respectively; P < 0.05) in POS compared with CON. The nonprotein RQ was greater in POS compared with CON due to greater (P < 0.05) oxidation of carbohydrate (213 vs. 115 kJ/kg of BW0.75 per day) compared with fat (362 vs. 486 kJ/kg of BW0.75 per day). Although no differences were found in C balance, goats in POS had lower (P < 0.05) amounts of C in CH4 (1.1 vs. 1.3 g/kg BW0.75 per day) compared with CON. There were no differences in N intake or N in feces or urine, but N in milk was greater (P < 0.05) in POS compared with the CON diet (0.8 vs. 0.7 g/kg BW0.75 per day). Yield of fat-corrected milk (FCM) (3.20 vs. 2.72 kg/d; P < 0.05) and concentration of true protein (3.4 vs. 3.3 kg/d; P < 0.05) and lactose (4.7 vs. 4.5 kg/d; P < 0.05) were greater in POS compared with CON. These responses were accompanied by lower (P < 0.05) urea (12.3 vs. 16.6 mM/L) and ammonia-N (6.6 vs. 8.8 mg/L) without changes in fat concentration (6.1% vs. 6.0%; P > 0.05) in POS compared with the CON diet. Daily amount of CH4 emission did not differ P > 0.05 between diets. However, when expressed relative to unit of edible product, feeding POS reduced (P < 0.05) the amount of CH4 by 46 g/kg of milk fat, 97 g/kg of milk protein, and 3 g/kg of milk compared with CON. Overall, data indicated that feeding a postbiotic in late-lactation increased energy efficiency for milk production partly by reducing CH4 emission.

Effects of laccase and lactic acid bacteria on the fermentation quality, nutrient composition, enzymatic hydrolysis, and bacterial community of alfalfa silage.

Ensiling has long been as a mainstream technology of preserving forage for ruminant production. This study investigated the effects of bioaugmented ensiling with laccase and Pediococcus pentosaceus on the fermentation quality, nutritive value, enzymatic hydrolysis, and bacterial community of alfalfa. The application of laccase and Pediococcus pentosaceus combination was more potent in modulating the fermentation quality of silage than laccase and Pediococcus pentosaceus alone, as indicated by higher lactic acid contents and lactic acid to acetic acid ratios, and lower pH, dry matter losses, and ammonia nitrogen contents. Moreover, treatments with additive enhanced protein preservation and structural carbohydrate degradation, while increasing true protein and water-soluble carbohydrate contents. By promoting lignin degradation, treatments containing laccase further facilitated the release of sugars from cellulose compared with treatment with Pediococcus pentosaceus alone. The additive treatments reduced the bacterial diversity and optimized the bacterial community composition of silage, with an increase in the relative abundance of desirable Lactobacillus and a decrease in the relative abundance of undesirable Enterobacter and Klebsiella. PICRUSt functional prediction based on Kyoto Encyclopedia of Genes and Genomes (KEGG) databases revealed that PL and LPL treatments increased the metabolism of membrane transport, carbohydrate, and terpenoids and polyketides related to fermentation activities. It can be concluded that bioaugmented ensiling with laccase and Pediococcus pentosaceus combination can be an effective and practical strategy to improve silage fermentation and nutrient preservation of alfalfa silage.

Effect of tea polyphenols on the fermentation quality, protein preservation, antioxidant capacity and bacterial community of stylo silage.

The present study was aimed to evaluate the improvement potential of tea polyphenols (TPP) on silage characteristics and bacterial community. Stylo ensiled with TPP (0, 0.2 or 0.4%, on a fresh basis) were analyzed for fermentation parameter, protein fraction, antioxidant activity and bacterial community after 7, 14, 30 and 60 days fermentation. The addition of TPP resulted in the decrease (P < 0.05) of pH values (5.09 vs. 4.91), dry matter loss (11.77 vs. 8.02% DM), butyric acid concentration (1.64 vs. 1.02% DM) and ammonia-N proportion (13.69 vs. 8.98% CP, on Day 30) of stylo silage as well as the increase (P < 0.01) of lactic acid bacteria population (6.17 vs. 7.54 cfu/g FM) and true protein content (6.03 vs. 7.44% DM), particularly at the first 30 days of ensiling. It somewhat enhanced the antioxidant capacity of style silage at the early stage, and altered the bacterial community of stylo silage, with Clostridium_sensu_stricto_1 and Lachnoclostridium_5 much decreased but Enterobacter and Clostridium still being the dominant genera. It is suggested that TPP could help improve fermentation quality and nutrient preservation of stylo silage, and delay proteolysis process and antioxidant decay.

Effects of an exogenous enzyme preparation extracted from a mixed culture of Aspergillus spp. on lactational performance, metabolism, and digestibility in primiparous and multiparous cows.

The objective of this study was to investigate the effects of an exogenous enzyme preparation from Aspergillus oryzae and Aspergillus niger on lactational performance of dairy cows. Forty-eight Holstein cows (32 primiparous and 16 multiparous) averaging (± SD) 36.3 ± 8.7 kg/d milk yield and 141 ± 52 d in milk were enrolled in a 10-wk randomized complete block design experiment (total of 24 blocks) and assigned to 1 of 2 treatments: basal diet, no enzyme supplementation (CON) or the basal diet supplemented with 4.2 g/kg dry matter intake (DMI) of an exogenous enzyme preparation containing amylolytic and fibrolytic activities (ENZ). After a 2-wk covariate period, premixes with the enzyme preparation or control were top-dressed daily by mixing with approximately 500 g of total mixed ration. Production data were collected daily and averaged by week. Milk samples were collected every other week, and milk composition was averaged by week. Blood, fecal, and urine samples were collected over 2 consecutive days at 0, 4, 8, 12, and 36 h after feeding during the last week of the experiment. Compared with CON, cows fed ENZ tended to increase DMI and had increased milk concentrations of true protein, lactose, and other solids. Milk fat content tended to be higher in CON cows. A treatment × parity interaction was found for some of the production variables. Primiparous cows receiving ENZ had greater yields of milk, energy-corrected milk, milk true protein, and lactose compared with CON primiparous cows; these production variables did not differ between treatments for multiparous cows. Intake and total-tract digestibility of nutrients did not differ between treatments. Concentrations of blood glucose and total fatty acids were not affected by ENZ supplementation, but β-hydroxybutyrate concentration tended to be greater in ENZ cows. Overall, the exogenous enzyme preparation used in this study increased milk protein and lactose concentrations in all cows, and milk production in primiparous but not multiparous cows. The differential production response between primiparous and multiparous cows was likely a result of a greater increase in DMI with ENZ supplementation in the younger animals.

Effects of Forestry Waste Neolamarckia cadamba Leaf Meal as an Additive on Fermentation Quality, Antioxidant Activity, and Bacterial Community of High-Moisture Stylo Silage

N. cadamba leaves, a byproduct of wood production, are always discarded in the field. N. cadamba leaves have strong antibacterial property, which might be recycled to inhibit undesirable bacteria and enhance the fermentation quality of silage. Ensiling, a traditional conservation method for animal feed, is commonly used all over the world. It is known that high-moisture forages, especially legumes, are difficult to ensile directly as much ammonia-N and butyric acid produced by undesirable bacteria will reduce the feeding value. To investigate the effects of N. cadamba leaf meal on the fermentation quality of stylo silage, 5% and 10% N. cadamba leaf meal were mixed with stylo for 30 days of ensiling in two independent experiments. Results showed that the silage pH and butyric acid content of stylo silage were decreased (p &amp;lt; 0.01) by 10% N. cadamba leaf meal. In experiment 2, contents of nonprotein-N and ammonia-N were significantly decreased (p &amp;lt; 0.05), while the true protein content was significantly increased (p &amp;lt; 0.05). The same results on the changing tendency were also obtained in experiment 1. In addition, N. cadamba leaf meal addition also decreased the bacterial diversity. The relative abundance of Clostridium and Lelliottia decreased, whereas that of Lactobacillus increased when N. cadamba leaf meal was added. It is worth noting that the addition of N. cadamba leaf meal also improved the antioxidant activity of stylo silage. The aforementioned results suggested that mixing N. cadamba leaf meal to high moisture forages could be an effective strategy to enhance silage fermentation quality, and it is also a feasible way to recycle N. cadamba leaves.

Ensiling and thermic treatment effects on nutrient content, protein solubility, and in vitro fermentation of partial crop faba beans

Legumes such as faba beans have a great potential to create local, independent, and high-quality protein resources for ruminant nutrition. Suitable methods for cost- and energy-effective preservation and protein stabilization against rapid fermentation in the rumen play an important role. Partial crop faba beans harvested with 500 or 708 g dry matter (DM)/kg were ensiled, toasted (160 °C, 60 min), or ensiled and toasted and it was investigated how this affects nutrient composition, protein solubility, carbohydrate fermentation, and in vitro organic matter digestibility (IVOMD). Non-protein nitrogen (NPN) concentrations increased and true protein (TP) concentrations decreased after ensiling, depending strongly on DM concentration of the plant (i.e., on maturity) – in NPN, from 15% to 35% (P < 0.001) and from 16% to 21% of crude protein (CP) (P > 0.05), and in TP, from 85% to 65% and from 83% to 79% of CP with 500 and 708 g DM/kg, respectively (P > 0.05) – whereas concentrations of soluble protein did not increase as much as expected. Toasting reduced soluble protein concentration in native faba beans from 56% to 29% and from 54% to 40% of CP with 500 and 708 g DM/kg, respectively (P < 0.001) and in ensiled faba beans from 58% to 38% and from 60% to 34% of CP with 500 and 708 g DM/kg, respectively, compared to the native material (P < 0.001). Ensiling and toasting slightly affected other crude nutrient or metabolizable energy concentrations, carbohydrate fermentation, and IVOMD.

Consumption of Animal Source Foods Is Associated With Differences in Breastmilk Energy and Macronutrient Composition in Rural Bangladesh

ObjectivesTo quantify associations of maternal consumption of animal source foods during lactation with maternal milk (MM) energy and macronutrient composition in a population with a high burden of childhood stunting.MethodsWe studied 99 mother-infant dyads from the Projahnmo pregnancy cohort in Sylhet, Bangladesh. Data on maternal diet was collected using a food frequency questionnaire administered at 1-month postpartum. Mothers reported how many times (never to daily) a food item was consumed in the past 7 days. We assessed intakes of red meat, liver, poultry, fish, eggs, and milk and milk products (sum of milk, yoghurt, milk powder, and khoa). Mothers collected their milk mid-feed at 1-month postpartum. True protein, fat, and total carbohydrate content of MM were determined using mid-infrared transmission spectroscopy (MIRIS AB, Uppsala, Sweden). Total energy was calculated using the Atwater general calorie conversion factor. To quantify associations of animal source food consumption with MM composition, we used linear regression analysis, adjusting for maternal body mass index, household wealth quintile, and days postpartum.ResultsMedian maternal age was 24 years. 31% of mothers were underweight and 15% were overweight/obese at enrollment. MM samples were collected at a median of 50 days postpartum. The median MM energy content was 22.9 kcal/oz. Median fat content was 4.7 g/dL, carbohydrate 7.8 g/dL, and protein 0.9 g/dL. In adjusted analyses, each serving/week of milk and milk products was associated with increased MM energy (β 0.33 kcal/oz; 95% CI 0.06, 0.60) and fat (β 0.13 g/dL; 95% CI 0.03, 0.23). Each serving/week of liver was associated with decreased carbohydrate (β −0.06 g/dL; 95% CI −0.11, −0.01) and increased protein (β 0.06 g/dL; 95% CI 0.02, 0.10) content. Each serving/week of red meat was associated with increased protein content (β 0.06 g/dL; 95% CI 0.01, 0.12). Intake of total animal source foods, poultry, fish, and eggs was not associated with MM composition.ConclusionsIntakes of some animal source foods were associated with MM composition at 1-month postpartum in rural Bangladesh. Increasing consumption of certain animal source foods during lactation could be an effective strategy to optimize MM composition, which may benefit infant growth outcomes in low-to-middle-income countries.Funding SourcesNICHD, Brigham Research Institute.

Feeding Calcium-Ammonium Nitrate to Lactating Dairy Goats: Milk Quality and Ruminal Fermentation Responses.

Simple SummaryCalcium-ammonium nitrate (CAN) has been extensively used as a potential methane inhibitor for ruminants; however, there is still a need for studies focused on investigating its effects on the fatty acid profile and antioxidant capacity of milk, especially from dairy goats. Therefore, we evaluated the effects of CAN on nutrient digestibility, ruminal fermentation, and milk quality of lactating Saanen goats. Treatments consisted of a control diet (without CAN), 10 g of CAN per kg of dry matter, and 20 g of CAN per kg of dry matter. Supplemental CAN did not affect feed intake, digestibility of nutrients, and most ruminal fermentation parameters. Yields and composition of milk were not affected, and minor treatment effects were observed on the milk fatty acid profile. Milk antioxidant capacity was altered by increased conjugated dienes and reduced thiobarbituric acid reactive substances, along with greater concentrations of nitrate and nitrite residues in milk. Calcium-ammonium nitrate can be fed to lactating dairy goats up to 20 g per kg of dry matter without negative effects on nutrient digestibility and milk composition; however, it increased the concentration of conjugated dienes in milk, which may induce its faster lipid oxidation.We aimed to investigate the effects of calcium-ammonium nitrate (CAN) fed to lactating dairy goats on dry matter (DM) intake, digestibility of nutrients, milk properties (composition, antioxidant capacity, fatty acid profile, and nitrate residues), and ruminal fermentation parameters. Twelve lactating Saanen goats averaging 98.5 ± 13.1 days in milk, 53.5 ± 3.3 kg of body weight, and 2.53 ± 0.34 kg of milk/day were randomly assigned in four 3 × 3 Latin squares to receive the following diets: a control group (without CAN) with 7.3 g/kg DM of urea (URE), 10 g/kg DM of CAN (CAN10), and 20 g/kg DM of CAN (CAN20). Each period lasted 21 days, with 14 days for diet adaptation and seven days for data and sample collection. The DM intake, digestibility of nutrients, yields of milk, 3.5% fat-corrected milk, and energy-corrected milk were not affected by treatments. Similarly, there were no treatment effects on the yields and concentrations of milk fat, true protein, and lactose, along with minor effects on milk fatty acid profile. Total antioxidant capacity in milk was unaffected by treatments; however, concentration of conjugated dienes increased, while thiobarbituric acid reactive substances in milk decreased linearly. Nitrate and nitrite residues in milk were elevated by treatments, while the total of volatile fatty acids and ammonia-N concentration in the rumen were unaffected. Collectively, feeding CAN (up to 20 g/kg of DM) to lactating dairy goats did not affect feed intake, nutrient digestibility, and milk composition; however, it may increase milk lipid oxidation, as evidenced by increased conjugated diene concentration.

Detoxification of corn stalk pretreated with calcium hydroxide for true protein accumulation

Alkaline pretreatment is essential in lignin degradation, but the inhibitors produced in this process affect microbial growth. To overcome the impacts of the phenolic compounds, detoxification was applied to corn stalk pretreated with calcium hydroxide.The results showed that ferulic acid degradation rate can reach 85.11% by laccase at the optimal conditions. Phanerochaete chrysosporium degraded most vanillin (77.19%) and p-hydroxybenzaldehyde (63.82%) but the degradation of ferulic acid (15.34%) was relatively weak. Laccase combined with Phanerochaete chrysosporium detoxified most of the phenolic compounds including 2- methoxy-4-vinylphenol (88.46%) and salicylic acid (58.13%) that hardly decompose alone after calcium hydroxide pretreatment in this study. These results inferred that Phanerochaete chrysosporium might generate some substance during the spore germination and growth period which may cooperate with laccase to decompose the phenolic compounds. After the fermentation of detoxified corn stalk by Neurospora crassa, the true protein content was increased by 2.73 times, and 21.17% lignin was degraded.

Milk-containing products with reduced antigenicity

Whey containing more than 50% of milk solids can be attributed to an insufficiently demanded raw material of the dairy industry. The restriction of its usage for food purposes is the high residual antigenicity of whey proteins. This work aimed to justify the possibility of using milk-containing products with whey protein hydrolysate, characterized by reduced residual antigenicity, in healthy and dietary diets. Experimental work was carried out at the All-Russian Scientific Research Institute of Dairy Industry, the Animal-Derived Food Technology department and the Core Facilities Centre "Control and Management of Energy-efficient Projects" at the FSBEI HE VSUET. Compositions were developed for a sports nutrition drink made from whey protein hydrolysate with the addition of fructose, and a milk-containing drink with the replacement of 30% skimmed milk necessary for standardization with whey protein hydrolysate. The chemical composition, physical, chemical and microbiological properties of the finished products were established. The reduction of the true protein content was proven to 1.33% for a sports nutrition drink and to 1.69% for a milk-containing drink. Peptides and proteins in the finished products have a molecular weight of less than 10 kDa, which indicates a decrease in their potential antigenicity. The developed beverages are characterized by a high content of biologically active peptides, macro- and microelements, as well as vitamins. Their quality and safety indicators meet the requirements of the TR CU 033/2013. These products can be used as part of dietary diets for people who are allergic to cow's milk proteins. The organization of their production will contribute to import substitution in the segment of low-allergenic dairy products.