True Anoestrus Research Articles

TREATMENT OF CLINICAL ANOESTRUS IN ARABIAN MARES BY A PROSTAGLANDIN F2 α|ή‎|‎ωϧΔΔ ‎‎|‎ ‎

A synthetic prostaglandin F2 α(PGF2 α) analogue, luprostiol* , was used in treatment of clinically anoestrus Arabian mares that suffered from abnormal persisting luteal function. Thirty two anoestrus mares were selected for this study after careful examination of their breeding history and a thorough genital examination which included rectal palpation of the genital tract and ovaries. The genital examination also included a vaginoscopy of the vagina and cervix. These mares were diagnosed as clinically anoestrus during the spring and summer months breeding season and received a single intramuscular injection of the prostaglandin F2 α. Twenty six mares (81%) responded to the treatment and came to oestrus within 2-4 days. Ovarian activity of the induced oestrus was monitored by rectal palpation and the mares were bred twice according to their follicular development. A total of 13 mares (54%) became pregnant as a result of the treatment and pregnancies were confirmed later by rectal palpation. This study reveals that the majority of non-cycling mares during the spring and summer months breeding season are in a prolonged luteal function rather than inactive ovaries or ‘true anoestrus. The study also demonstrates the efficiency of Iuprostiol in the treatment of clinically anoestrus mares.

Evaluation and Comparison of Therapeutic Efficacy of Various Hormonal Protocols in Anoestrus Buffalo Heifers of Semi-Arid Region

To assess and compare different hormonal protocols' treatment efficacy in anoestrus buffalo heifers in a semi-arid region. Forty buffalo heifers with a history of anoestrus were chosen randomly from the semi-arid region of Banaskanth and divided into four equal groups.Group-I: Heatsynch protocol (n = 10), Group-II Ovsynch protocol (n = 10), Group-III Doublesynch protocol (n = 10) and Group-IV Control (n = 10) are followed by artificial insemination at a predetermined period in treated buffalo heifers and insemination during spontaneous estrus in group-IV buffalo heifers. In Groups I, II, and III, the corresponding percentages of estrus induction were 90, 80, and 80 percent. Major symptoms such as edoema, erythema, dampness of vulva and estrus mucus secretion were present during the expression of estrus. Fifty percent of the buffalo heifers in Group IV displayed estrus at varying intervals. After three consecution services, Group II had the highest overall fertility rate (50%) followed by Group III (40%), Group I (30%), and Group IV (30%). In true anoestrus buffalo heifers, the Heatsynch, Ovsynch, and Doublesynch procedure can be applied with success to induce estrus. Buffalo heifers that follow the Ovsynch protocol may have a higher conception rate.

Blood Plasma Biochemical Changes and Conception Rate in Anoestrus Buffaloes Treated with Various Hormone Protocols

To study certain blood biochemical parameters (total cholesterol, total protein, calcium and inorganic phosphorus) at various periods of treatment. Total 40 anestrous buffalo heifers with history of anoestrus were selected from the semi-arid area of Banaskanth and randomly divided into four equal groups. (1)Group-I:Heatsynch protocol (n=10), (2)Group-II:Ovsynch protocol(n=10), (3)Group-III: Doublesynch protocol (n=10) and (4)Group-IV: Control(n=10) followed by fixed time artificial insemination in treated buffalo heifers and insemination at spontaneous estrus in buffalo heifers of group-IV. The overall conception rate following 3 consecution services was maximum in Group II (50%) followed by Group III (40%), Group I (30%) and Group IV (30%). The blood plasma was collected at the time of initiation of treatment (P1), at the time of inj. PGF2α (P2), at the time of 1st FTAI (P3) and day 21st post insemination (P4) for the estimation of biochemical parameters. The blood biochemical profiles include total protein (range: 4.54± 0.19 to 6.11± 0.52 g/dl) which in all group did not show any significant difference during the different period but significant (P<0.05) difference between the groups was noticed. Similarly calcium and phosphorus found ranging between 5.73± 0.20 to 9.53± 0.95 mg/dl and 5.46± 0.22 to 6.95± 0.30 mg/dl, respectively in all four groups. The cholesterol estimated values (range: 34.12 ± 1.75 to 41.97± 2.43 mg/dl) were found not-significantly varying between the groups as well as among the different periods. Heatsynch, Ovsynch and Doublesynch protocol can be successfully used for the induction of estrus in true anoestrus buffalo heifers with Better conception rate would be achieved following Ovsynch protocol in buffalo heifers.

Assessment of Haematological, Biochemical and Hormonal Changes in Mehsana buffaloes with Postpartum True Anestrus following Herbal Heat Inducer Treatment

The present study was designed to explore the effects of a new Herbal heat inducer in Mehsana buffaloes with postpartum true anestrus and consecutive changes in Blood haematological, Biochemical and Hormonal profile. All the buffaloes were orally supplemented with Herbal heat inducer mixture on (Jantana powder-10gm) 1st, (Metrali powder-20gm) 2nd and (Hitali-powder-500mg) 5th day for augmentation of oestrus. Out of total treated animals 10 animals expressed the signs of estrus within 30 days. Post-treatment analysis of the overall blood packed cell volume percent in conceiving buffaloes had a significant (p≤0.05) elevate. Overall blood plasma total cholesterol concentration (mg/dl) was found to be significantly (p≤0.001) elevated in all treated and both conceiving and non-conceiving buffaloes, respectively. Overall blood plasma progesterone concentration (ng/ml) was found to be significantly (p≤0.0001) and (p≤0.001) elevated in all treated and both conceived and non-conceived buffaloes, which indicate the positive favourable effect of all the drugs on postpartum true anestrus condition. However, Overall blood plasma tri-iodothyronine concentration (ng/ml) was found to be significantly (p≤0.001) and (p≤0.01) elevated in all treated and all conceived buffaloes. Findings of the present study revealed that strategic use of Indigenous herbal heat inducer in feed mixture may resolve the postpartum true anestrus problems in buffaloes.

Prevalence of single and multiple reproductive disorders in Jersey crossbred cows of sub-tropical North-Western Himalayas

A total of 1612 cows during 118 rural infertility camps (October 2013 to February 2021) in Himachal Pradesh were examined for prevalence of single and multiple reproductive disorders. Subsequently, prevalence of reproductive disorder was analysed from a perspective of age (Group 1: ≤2.5 years; Group 2: 2.5-7 years; Group 3: ≥7 years) and season (summer, monsoon, post-monsoon andwinter). In descending order, true anestrus, endometritis, infantile genitalia, repeat breeding and silent estrus were most prevalent single disorders. However, prolonged estrus with endometritis (62.3%) and metestral bleeding with repeat breeding (29.8%), respectively were concomitantly present as multiple disorders (in the same set of cows). Group 2 cows were highly susceptible for the presence of single and multiple disorders; whereas the disorders were most prevalent during pre-winter-winter and minimumduring summer season.

Prevalence of single and multiple reproductive disorders in Jersey crossbred cows of sub-tropical North-Western Himalayas

A total of 1612 cows during 118 rural infertility camps (October 2013 to February 2021) in Himachal Pradesh were examined for prevalence of single and multiple reproductive disorders. Subsequently, prevalence of reproductive disorder was analysed from a perspective of age (Group 1: ≤2.5 years; Group 2: 2.5-7 years; Group 3: ≥7 years) and season (summer, monsoon, post-monsoon andwinter). In descending order, true anestrus, endometritis, infantile genitalia, repeat breeding and silent estrus were most prevalent single disorders. However, prolonged estrus with endometritis (62.3%) and metestral bleeding with repeat breeding (29.8%), respectively were concomitantly present as multiple disorders (in the same set of cows). Group 2 cows were highly susceptible for the presence of single and multiple disorders; whereas the disorders were most prevalent during pre-winter-winter and minimumduring summer season.

Effect of Mineral Mixture Supplementation on Haemato-biochemical Parameters of Anoestrus Cows in Winter and Summer Season

Background: livestock is mainly reared under a crop livestock integrated farming system and mineral supplementation is not common practice. Out of all nutrients, minerals and vitamins play a crucial role in metabolism, lactation, reproduction and even for microbial fermentation in rumen. Considering the mineral deficiencies in particular zones, the area specific mineral mixtures (ASMM) were formulated for various zones and also found various limiting nutrients in different zones of MP. The current study was planned to find out the effect of mineral mixture supplementation on body weight, body condition score and haemato-biochemical profile of non-descript anoestrus cows. Methods: A total-forty-eight animal were selected out of the surveyed animals and divided into four groups (n=12) viz. T1 (Normal cyclic animals), T2 (Anoestrus Control), T3 (True anoestrus) and T4 (Sub-oestrus). The experiments were carried out in two seasons (summer and winter). All physiological and hemato-biochemical parameters were recorded and analyzed as per standard mentioned protocol. Result: According to the findings of this experiment weight gain the animals of the true anoestrus and sub-oestrus group’s recorded highly significant increases in the winter and summer seasons. The haemoglobin concentration was within the normal range and it was non-significant in all groups except normal cyclic animal (T1) in winter and true anoestrous (T3) in summer after the mineral supplementation. There was no significant difference in the WBC count of all groups except in sub-oestrous control (T2S) and T1 in the winter season. RBC concentration was non-significant in almost all groups. Erythrocytic indices were the normal limits and did not differ significantly in the majority of groups except significant difference showed are MCHC in T3, MCH in T2S, MCV in T1 of winter seasons and were within normal range in all supplemented groups in both. The serum cholesterol concentration showed significantly higher value in the winter and summer season of T3 group. However, in sub-estrous (T4 group) animals, the values in the winter season were significantly higher. We observed significantly higher values of triglycerides in T3 and T4 groups in winter and T3, T4 and T1 groups in summer seasons. The above results indicate the beneficial effects of mineral mixtures in supplement groups. Studies conclude that the supplementation of mineral mixtures can improve reproductive efficiency in non-descript anoestrus cows.

Efficacy of Second Prostaglandin F2 Alpha Treatment during Ovsynch Protocol on Pregnancy Rate Inseminated with Sex-sorted Semen in Buffaloes

Background: Ovsynch is one of the most adopted estrus synchronization protocols used for synchronization of ovulation and fixed-time artificial insemination however, new corpus luteum (CL) induced by the first GnRH treatment in Ovsynch is inappropriately regressed with a single treatment with prostaglandin F2α. Inadequate CL regression reduces fertility. Considering this rationale, present research was implemented with the effect of second prostaglandin F2 alpha treatment during Ovsynch protocol on pregnancy rate inseminated with sex-sorted semen in buffaloes. Methods: Post-partum true anestrus multiparous buffaloes (n=24) were categorized as a group I (Ovsynch; n=12) and group II (Double PG Ovsynch; n=12). Buffaloes from group I were treated with standard Ovsynch protocol (D=7; G-P-G), while additional second prostaglandin F2 alpha injection was administered on day eighth (D=7; G-P-P-G) in group II (Double PG Ovsynch). All the buffaloes were inseminated 16-20 hrs after the last GnRH injection with sex-sorted semen. Result: The estrus signs exhibited were numerically higher in group II than in group I. The first service pregnancy rate in double PG Ovsynch was higher than the Ovsynch group. The addition of a second prostaglandin F2 alpha injection in the Ovsynch protocol improves the estrus quality and increases pregnancy rate.

Ultrasonographical and hormonal comparative between true and postpartum anestrus of cows in south of Iraq

Anestrus is one of the major reproductive problems. A total of 218 local breed cows were diagnosed as 105 cows with true anestrus and 113 cows with postpartum anestrus. The results indicated that most cases of postpartum anestrus were concentrated from December to May, while the true anestrus occurred from May to September. The most important causes that were diagnosed by ultrasound for true anestrus cases were inactivity of the ovaries and anovulatory follicles. A high percentage of postpartum anestrus cases were suffered from luteinizing cysts, ovarian inactivity. Hormonal assay for inactive ovaries in true and postpartum anestrus cases revealed a significant decrease (p<0.05) in the levels of estrogen, progesterone, FSH and LH. While the hormonal results of postpartum anestrus cases revealed a significant decrease (p<0.05) in the estrogen with a significant increase (p<0.05) in the progesterone in case of luteal cyst, persistent corpus luteum and cystic corpus luteum which accompanied by a significant increase (p<0.05) in the prolactin .The ultrasonographical features for true anestrus showed an increase in echo rates for ovaries. In postpartum anestrus cases the luteal cyst revealed 2.88-2.90cm follicle like structure. While the cases of persistent corpus luteum showed 1.15cm-1.75cm dense theca tissue.

Augmenting Fertility in Anestrus and Subestrus Cows with Hormonal Interventions and Their Effect on Blood Biochemical Profile

A study was carried out on forty-four problem breeder cows to evaluate the therapeutic efficacy of GnRH and PGF2α for estrus induction response, treatment to estrus induction interval and conception rate and its effect on biochemical profile. Thirteen true anestrus cows were treated with GnRH (Inj. Receptal, 2.5 ml, IM, once), twenty three subestrus cows were treated with PGF2α (PGF2α, Inj. Estrumate, 2 ml, IM, once) and eight animals kept as untreated anestrus control. The estrus induction response, treatment to estrus induction interval, overall conception rate and service per conception in GnRH treated, PGF2α treated and positive control cows were 84.62%, 28.27±6.46 days, 72.73%, 3.13; 91.30%, 3.52±0.46 days, 71.43%, 2.53 and 50%, 48.25±10.8 days, 50%, 3.5 respectively. The plasma P4 level was significantly lower in all three groups as compared to other two periodic values (d-0 & d-20-22 post AI) which were due to luteal demise. The mean serum total protein was significantly (p<0.05) lower in untreated anestrus (8.57±0.36 g/dl) as compared to GnRH (9.75±0.28 g/dl) and PGF2α (9.62±0.31 g/dl) treated cows. However, total cholesterol was numerically lowest in control cows than those of treated contemporaries but did not differ significantly (168.22±17.22; 208.46±14.71; 163.84±10.64 mg/dl; in GnRH, PGF2α and control group respectively, p<0.05). In conclusion, anestrus and subestrus cows can be well managed with GnRH and PGF2α therapy. The normal hormonal and biochemical milieu is essential for normal functioning of reproductive system.

Herbal Combo Therapy’ for Oestrus Induction in Postpartum Anoestrus Cows

The research was aimed at studying the effect of a ‘Herbal combo therapy’ (HCT) on induction of oestrus and enhancement of fertility in postpartum anoestrus (PPA) in crossbred cows. Crossbred cows (n = 24) which were diagnosed for true anoestrus condition were utilized for the study. All the cows were dewormed and randomly divided into two groups (i) Control group (n = 10): No treatment schedule and (ii) Treatment group (n = 14): HCT involving a sequential administration of Raphanus sativus, Aloe vera, Moringa oleifera, Cissuss quadrangularisis and Murraya koenigii for a period of 20 days. The animals which expressed oestrus signs during the experimental period were inseminated and pregnancy was confirmed. Diameter of the largest follicle was documented on the initiation day of experiment and at the end of the experimental period or on the day of expression of induced oestrus. Perusal of the data revealed that 71.4 per cent of the PPA animals responded to the HCT by expressing oestrus signs within the experimental period as against 10.0 per cent in control group. The follicular diameter significantly increased in HCT group (11.8 + 2.4 mm) than the control group (9.6 + 0.8 mm). The overall conception rate among the PPA cows treated with HCT was much higher (57.1%) than the control group (10.0%). Steroidogenic activity, rich nutrient source and anti-inflammatory properties of HCT would have potentiated the follicular development and thereby induced oestrus and improved conception rate in anoestrus cows.

Ovarian Follicular Dynamics, and Hormonal and Biochemical Profile in Post-Pubertal and Postpartum Jaffrabadi Buffaloes

Jaffrabadi buffalo is one of the heaviest buffalo breeds of the world (800 kg) and is known for poor reproductive efficiency. There is a scarcity of normal physiological data regarding reproductive parameters of Jaffrabadi buffalo. The primary objective of this study was to elucidate ovarian follicular dynamics, hormonal and biochemical profiles in 10 post-pubertal heifers (n=5 each of age 42±2 and 48±2 months) and five >90 days postpartum lactating buffaloes. Ultrasound monitoring of ovaries of animals was done at weekly interval and all follicles with diameter less than 4 mm (small follicles), 4-8 mm (medium follicles) and greater than 8 mm (large follicles) as well as CLs identified were recorded. Blood samples were also collected soon after each USG from all animals for assay of hormonal and biochemical profiles. The findings revealed that the average number of small, medium and large follicles, total number of follicles per ovary as well as diameters of largest and subordinate follicles varied greatly among periods between three groups. The size of corpus luteum ranged from 13.7 to 21.2 mm, 13.7 to 23.9 mm and 15.3 to 22.7 mm, respectively, in Group-I, II and III. In the first week of study, significantly higher (p less than 0.05) average numbers of small follicles in Group-I and III, and medium follicles and total numbers of follicles in Group-III were observed as compared to other groups. The diameter of largest follicles and subordinate follicles were non-significantly different among all groups at all periods of the study. Plasma estrogen levels were significantly higher (p less than 0.05) in Group-I as compared to Group-III and Group-II on first and second week, however, it increased significantly (p less than 0.05) in Group-II and I on fourth week of study period. Plasma insulin concentrations were significantly (p less than 0.01) lower in buffaloes (Group-III) than in heifers (Group-I and II) throughout the study period, while plasma total cholesterol and total protein levels were higher in Group-III on 3rd to 5th weeks of study. Further, there were no significant differences in the plasma FSH, LH, progesterone and blood glucose levels in animals of three groups between periods at any of the week. The ultrasonographic and endocrine profile suggested establishment of cyclicity with silent ovulation. However, the behavioural estrus was still not manifested by these post-pubertal heifers and postpartum lactating buffaloes. These results are helpful to diagnose anovulatory conditions and/or true anestrus and initiate estrus synchronization protocols for early conception in Jaffrabadi buffaloes.

Fertility response of anestrous buffaloes to eCG2-Modified or Norgestomet plus eCG6- Modified-GnRH-based ovsync protocols during the breeding season.

Objective: The aim of the current study was to evaluate the fertility response of anestrous buffaloes and associated follicular growth and hormonal profiles to eCG-Modified (eCG2-ovsync) or eCG plus Norgestomet ear implant-Modified (Norgestomet-eCG6-ovsync) GnRH-Based ovsync protocols. Methods: Buffaloes (n=42) were randomly allocated into three treatment protocols: Basic-ovsync, Norgestomet-eCG6-ovsync and eCG2-ovsync (14 each). Each buffalo-cow in the Basic-ovsync protocol received two GnRH agonist injections on Days 0 (GnRHa1) and Day 9 (GnRHa2) and an injection of PGF2α on Day 7 with TAI at 16 h after GnRHa2 injection. Buffaloes in Norgestomet eCG6-ovsync (n=14) received the same treatment as Basic –ovsync plus IM injection of 250 mg of long-lasting progesterone and S/C insertion of Norgestomet ear implant in the ear Conca on Day 0 and an injection of 500 IU eCG on Day 6. Buffaloes in eCG2-ovsync received the same treatment of Basic ovsync in addition to an injection of 1000 IU eCG on Day 2. Simultaneous transrectal ultrasonography (TUS) and blood sampling were performed on Days -10, 0, 7, 9 and 10 as well as on post-TAI Days 11 and 22. Results: The largest follicle diameter (LFD) was significantly larger (p < 0.05) in eventually diagnosed pregnant (EDP) than in eventually diagnosed non-pregnant (EDnP) buffaloes in all treatment protocols on Day 9. The serum progesterone (P4) concentration was significantly higher (p < 0.05) in either EDP or EDnP buffaloes in Noegestomet eCG6-ovysync compared with other protocols on Day 7. There was a highly significant (r=0.74, P<0.01) positive correlations between corpus luteum volume (CLV) and LFD in EDP buffaloes. Conclusions: It is concluded that modifying Basic-ovsync via P4 supplementation by injection of long-lasting P4 on Day 0 and insertion of Norgestomet ear implant from day 0 to day 7 plus an injection of eCG on Day 6 improve the fertility response in true anestrus in buffaloes.

Study on Relationship of Soil, Plant and Serum Minerals Concentration in Cyclic and Anestrus Buffaloes

The present study was carried out to study the relationship of concentration of minerals in soil, plant and in cyclic and anestrus buffaloes. A total of 22 anestrus and 5 cyclic buffaloes were utilized for the study. The concentration of minerals i.e. Cu, Fe, Zn, Mn, Ca and P was studied in soil, plants and serum of buffaloes. The ration of Ca:P was disturbed in anestrus buffaloes. The concentration of Cu, Fe, Zn, Mn and P were significantly lower compared to plants. The concentration of Cu, Zn, Mn, Ca and P in the true anestrus and normal cyclic buffaloes were within the normal range. However, the concentration of Fe in true anestrus and normal cyclic buffaloes were higher compared to normal range.

Ultrasound guided diagnosis of anoestrus and its treatment in postpartum crossbred Holstein-Friesian cows

This study was undertaken to determine the feasibility of using ultrasonography for diagnosis of postpartum anoestrus followed by its effective management and treatment in crossbred (Holstein-Friesian X zebu) cows. A total of 57 crossbred lactating cows with unobserved oestrus for ≥60 days postpartum was examined by ultrasonography at Central Cattle Breeding and Dairy Farm (CCBDF), Savar, Dhaka. Cows were divided into silent oestrus and true anoestrus groups by ultrasonography. The pregnancy was diagnosed transrectally using ultrasonography 28 - 35 days after AI. Cows with silent oestrus were treated with Cloprostenol (500 μg) followed by AI or with 2 doses of Cloprostenol 10 days apart followed by AI at observed oestrus. The true anoestrus cows were supplied with anthelmintic and vitamin ADE injection with balanced nutrition, treated with Gonadorelin (500 μg) followed by AI or with Gonadorelin followed by injecting Cloprostenol at 7 days interval and AI at observed oestrus. All cows with silent oestrus and true anoestrus were divided into different body condition score (BCS), milk yield, age and parity groups to determine their influence on outcome of treatment. Out of 57 anoestrus cows examined, 56% were confirmed as silent oestrus and 44% were confirmed as true anoestrus. In silent oestrus, 71% cows showed cyclicity and 43% became pregnant after treatment with Cloprostenol and 78% cows showed cyclicity and 50% became pregnant after treatment with 2 doses of Cloprostenol 10 days interval (P&gt;0.05). In true anoestrus group, 60% cows showed cyclicity and 40% became pregnant when balanced diet was supplemented. Around 78% cows showed cyclicity and 44% became pregnant after treatment with Gonadorelin and 91% cows showed cyclicity and 55% became pregnant when Gonadorelin was followed by Cloprostenol at 7 days interval (P&gt;0.05). Significant (P&lt;0.05) influence of parity was observed in post-treatment cyclicity in true anoestrus cows. The Bangladesh Veterinarian (2019) 36(1 - 2): 33-41

Dynamics of Selected Trace Minerals during Estrus Induction in Anestrus Sahiwal Cattle

The present study evaluated the effect of circulatory trace mineral profiles on fertility response during estrus induction by progesterone based therapy in acyclic Sahiwal cattle. Sahiwal cows and post-pubertal heifers (n=25 each) with true anestrus condition (acyclicity confirmed ultrasonographically) were subjected to CIDR-based protocol (intravaginal CIDR application for 7 days, 500 IU eCG at CIDR removal, followed by estrus observation and AI). In heifers, additionally an injection of 5.0 mg estradiol valerate at CIDR insertion was given. Blood samples on days 0 (before CIDR application), 3, 5, 7 and 9 were collected. Almost similar estrus induction (68% vs. 64%) and subsequent conception rates (41.2% vs. 50%) during the first induced estrus were recorded in cows and heifers, respectively. During the course of treatment, significant (P 0.05) was recorded for iron concentration vis-a-vis estrus induction response. Hence, it is indicated that circulatory copper, zinc and manganese profiles influence the reproductives energy in acyclic animals and their suboptimal profiles may reduce the efficacy of estrus induction protocols in Sahiwal cattle.

Studies on Factors Affecting Pregnancy Rate after Treatment of Anestrus Buffaloes in Chitwan District

This study was designed to determine the effects of age, parity, feeding practice, body condition score (BCS), blood nutritional parameters, types of anoestrus, and length of anoestrus on pregnancy rate of anoestrus buffaloes after their treatment with ovsynch protocol followed by fixed time artificial insemination. Blood samples were collected on the day ovsynch protocol and analyzed. The mean values of the nutritional parameters between different groups were compared by Students t test and pregnancy rate was compared by Fisher’s exact test or Chi-square test. The overall pregnancy rate in buffaloes was 28.6%, when checked on day 41 and 64.3% on day 102 of FTAI. Blood glucose, cholesterol, calcium and phosphorus level was found to be significantly higher (p&lt;0.05) in pregnant than in non-pregnant buffaloes. Pregnancy rate was higher in silent estrus buffaloes. Buffaloes with BCS ≥3 had higher pregnancy rate than those with BCS &lt; 3. Similarly, pregnancy rate was higher in lactating than in non lactating buffaloes. While young buffaloes of age &lt;10 year responded better than old buffaloes of age ≥10 year. Buffaloes which were free from gastrointestinal parasitic infection had higher pregnancy rate. In conclusion, ovsynch protocol produces a good pregnancy in anoestrus buffaloes; however state of true anoestrus, older age and poor BCS with poor nutritional management adversely affect the pregnancy outcome. The role of blood nutritional parameters requires further clarification.

Causes and therapeutic management of infertility in abandoned cows of Himachal Pradesh

The present study was carried out with an objective of examining the reproductive status of abandoned cows kept in 18 goshalas of Himachal Pradesh. Overall 321 abandoned cows were diagnosed with 402 reproductive ailments (few of them with multifactor etiology). Amongst the genital tract ailments in abandoned cows, a very high incidence, i.e. 50%, of reproductive disorders was associated with injuries/ technical errors (endometritis, cervical disorders, affections of fallopian tubes and adnexa) of which only 12.75% were treatable. However, 38.80% incidence of managemental/ functional problems (true anestrus and silent estrus) was found and a higher post treatment recovery rate was recorded. This can be attributed to the faulty AI technique, inefficient inseminators or inadequate training on AI. Overall, 22.86% abandoned cows having various reproductive ailments conceived following different therapeutic regimes.

Identification and clinico-gynaecological characterization of reproductive disorders in crossbred cows under field conditions

The present study was conducted on a total of 909 crossbred cows maintained in various private farms from Kamrup, Darrang and Lakhimpur districts of Assam. Breeding records provided by the farmers and characteristic genital changes detected on per rectum examination of 133 reported infertile cows were analyzed. The study revealed that the incidence of infertility due to different types of reproductive disorders was 14.63 per cent in cross bred cow under field conditions. Common clinically detectable reproductive disorders were repeat breeding with uterine infection, repeat breeding without uterine infection, true anoestrus and silent oestrus with the percentage frequencies of 36.09, 23.30, 20.30 and 17.29 respectively when estimated out of total number of infertile cows. Less frequent reproductive disorders were pyometra, ovarian cyst, infantile genitalia and ovario-bursal adhesion occurring in the frequencies of 36.09, 23.30, 20.30 and 17.29 per cent respectively. Irrespective of type the incidence of anoestrus and repeat breeding was 37.59 and 59.39 per cent respectively. Palpable corpus luteum was present in the ovary of silent oestrous cows but totally absent in cows affected with true anoestrus. Mucopurulent vaginal discharge was a distinct clinically detectable genital characteristic observed in 62.50 per cent repeat breeder cows with uterine infection. Of the infertile cows 53.38 per cent had body condition score between 2.5 and 3.5 and 46.61 per cent had the score less than 2.5. Out of repeat breeder cows with uterine infection 43.66 per cent had good body condition and 27.41 per cent poor body condition. Level of serum calcium, zinc, leptin, ghrelin and IGF-1 did not vary significantly between types of infertility. Level of phosphorus and iron was lower in cows affected with repeat breeding due to infection. Serum oestrogen level remained low in cows affected with silent oestrus and true anoestrus while level of progesterone was higher in cows affected with silent oestrus.

Studies on Factors Affecting Pregnancy Rate after Treatment of Anestrus Buffaloes in Chitwan District

This study was designed to determine the effects of age, parity, feeding practice, Body Condition Score (BCS), blood nutritional parameters, types of anoestrus, and length of anoestrus on pregnancy rate of anoestrus buffaloes after their treatment with ovsynch protocol followed by fixed time artificial insemination. Blood samples were collected on the day ovsynch protocol and analyzed. The mean values of the nutritional parameters between different groups were compared by Students t test and pregnancy rate was compared by Fisher’s exact test or Chi-square test. The overall pregnancy rate in buffaloes was 28.6%, when checked on day 41 and 64.3% on day 102 of FTAI. Blood glucose, cholesterol, calcium and phosphorus level was found to be significantly higher (p&lt;0.05) in pregnant than in non-pregnant buffaloes. Pregnancy rate was higher in silent estrus buffaloes. Buffaloes with BCS ≥3 had higher pregnancy rate than those with BCS &lt; 3. Similarly, pregnancy rate was higher in lactating than in non-lactating buffaloes. While young buffaloes of age &lt;10 year responded better than old buffaloes of age ≥10 year. Buffaloes which were free from gastrointestinal parasitic infection had higher pregnancy rate. In conclusion, ovsynch protocol produces a good pregnancy in anoestrus buffaloes; however, state of true anoestrus, older age and poor BCS with poor nutritional management adversely affect the pregnancy outcome. The role of blood nutritional parameters requires further clarification.&#x0D; Int. J. Appl. Sci. Biotechnol. Vol 7(2): 248-256&#x0D;