Trout Swimming Research Articles

The influence of wall effects on self-propelled performance of brown trout swimming

When benthic fish engage in predation, they often swim near the riverbank or close to hard rocks, where they are subjected to the combined effects of side and benthic walls. This study focuses on the brown trout and employs a three-dimensional numerical model to simulate the process of brown trout accelerating from a stationary state to a cruising state under the influence of wall effects. A self-developed subroutine algorithm is applied to solve the various hydrodynamic parameters of brown trout swimming. By varying the distance between the fish's center of gravity and the wall, this study explores the self-propelled performance and efficiency of fish swimming affected by the sidewall as well as the combined influence of the side and benthic walls. This study also reveals the hydrodynamic mechanism of wall effects that impact the performance of fish swimming in the body/caudal fin (BCF) mode. The results demonstrate that when the distance is less than 0.5 times the body length of the fish, swimming near the sidewall can enhance speed and thrust, but the swimming efficiency will reduce. Closer proximity to the benthic wall leads to increased power consumption and decreased efficiency, which is disadvantageous for fish swimming. The findings of this study reveal the unstable wall effects experienced by fish and offer insights for designing biomimetic underwater vehicles that leverage wall effects as well as for creating habitats that support fish swimming in the BCF mode.

CFD Investigation of Trout-Like Configuration Holding Station near an Obstruction

This report presents the development of a fluid-structure interaction model using commercial Computational fluid dynamics software and in-house developed User Defined Function to simulate the motion of a trout Department of Mechanical Engineering, Widener University holding station in a moving water stream. The oscillation model used in this study is based on the observations of trout swimming in a respirometry tank in a laboratory experiment. The numerical simulations showed results that are consistent with laboratory observations of a trout holding station in the tank without obstruction and trout entrained to the side of the cylindrical obstruction. This paper will be helpful in the development of numerical models for the hydrodynamic analysis of bioinspired unmanned underwater vehicle systems.

Wireless logging of extracellular neuronal activity in the telencephalon of free-swimming salmonids

BackgroundSalmonids return to the river where they were born in a phenomenon known as mother-river migration. The underpinning of migration has been extensively examined, particularly regarding the behavioral correlations of external environmental cues such as the scent of the mother-river and geomagnetic compass. However, neuronal underpinning remains elusive, as there have been no biologging techniques suited to monitor neuronal activity in the brain of large free-swimming fish. In this study, we developed a wireless biologging system to record extracellular neuronal activity in the brains of free-swimming salmonids.ResultsUsing this system, we recorded multiple neuronal activities from the telencephalon of trout swimming in a rectangular water tank. As proof of principle, we examined the activity statistics for extracellular spike waveforms and timing. We found cells firing maximally in response to a specific head direction, similar to the head direction cells found in the rodent brain. The results of our study suggest that the recorded signals originate from neurons.ConclusionsWe anticipate that our biologging system will facilitate a more detailed investigation into the neural underpinning of fish movement using internally generated information, including responses to external cues.

Thermal acclimation of rainbow trout myotomal muscle, can trout acclimate to a warming environment?

Climate change is a looming threat to the planet. Cold-water aquatic species will face significant physiological challenges due to elevated summer temperatures. Salmonids, such as rainbow trout (Oncorhynchus mykiss) maintain fidelity to native streams, limiting their ability to mitigate the impact of climate change through migration. We examined how rainbow trout swimming performance and muscle function were shaped by the thermal environment. We hypothesized that trout would show slower muscle contractile properties and slower swimming performance with long-term exposure to warmer water. For fish held at either 10 °C or 20 °C, maximum steady swimming speed (Ucrit) was determined, and contractile properties of both fast-twitch (white) and slow-twitch (red) myotomal muscle were examined. In addition, immunohistochemistry and quantitative PCR were used to assess changes in myosin content of the myotomal muscle in response to holding temperature. Rainbow trout exposed to warm water for six weeks displayed relatively limited thermal acclimation response. When tested at a common temperature (10 °C), 20 °C acclimated fish had modestly slower muscle performance compared to 10 °C acclimated fish. Significant differences in swimming performance and muscle contractile properties were primarily at colder test temperatures (e.g. 2 °C for muscle mechanics). Shifts in myosin heavy chain protein composition and myosin heavy chain gene expression in the swimming muscle were observed in white but not red muscle. Our results suggest that rainbow trout will have a limited ability to mitigate elevated environmental temperature through thermal acclimation of their myotomal or swimming muscle.

Extreme temperature combined with hypoxia, affects swimming performance in brown trout (Salmo trutta).

Climate change is predicted to impact freshwater aquatic environments through changes to water temperature (Twater), river flow and eutrophication. Riverine habitats contain many economically and ecologically important fishes. One such group is the migratory salmonids, which are sensitive to warm Twater and low O2 (hypoxia). While several studies have investigated the independent effects of Twater and hypoxia on fish physiology, the combined effects of these stressors is less well known. Furthermore, no study has investigated the effects of Twater and O2 saturation levels within the range currently experienced by a salmonid species. Thus, the aim of this study was to investigate the simultaneous effects of Twater and O2 saturation level on the energetics and kinematics of steady-state swimming in brown trout, Salmo trutta. No effect of O2 saturation level (70 and 100% air saturation) on tail-beat kinematics was detected. Conversely, Twater (10, 14, 18 and 22°C) did affect tail-beat kinematics, but a trade-off between frequency (ftail) and amplitude (A, maximum tail excursion) maintained the Strouhal number (St = ftail• A/U, where U is swimming speed) within the theoretically most mechanically efficient range. Swimming oxygen consumption rate (n}{}{dot{M}}_{{mathsf{O}}_{mathsf{2}}}) and cost of transport increased with both U and Twater. The only effect of O2 saturation level was observed at the highest Twater (22°C) and fastest swimming speed (two speeds were used—0.6 and 0.8 m s−1). As the extremes of this study are consistent with current summer conditions in parts of UK waterways, our findings may indicate that S. trutta will be negatively impacted by the increased Twater and reduced O2 levels likely presented by anthropogenic climate change.

Remote physiological monitoring provides unique insights on the cardiovascular performance and stress responses of freely swimming rainbow trout in aquaculture

Investigating the mechanisms that fish employ to maintain homeostasis in their everyday life requires measurements of physiological and behavioural responses in the field. With multivariate bio-loggers, we continuously measured gastrointestinal blood flow (GBF), heart rate, activity and body temperature in rainbow trout (Oncorhynchus mykiss) swimming freely amongst ~5000 conspecifics in a sea cage. Our findings clearly demonstrate that while both acute aquaculture-related stress and spontaneous activity resulted in transient reductions in GBF (i.e. reductions of up to 65%), recovery from stressful handling practices subsequently involved a substantial and prolonged gastrointestinal hyperemia far beyond the level observed prior to the stressor. The gastrointestinal hyperemia may be necessary to repair the damage to the gastrointestinal tract caused by acute stress. Furthermore, heart rate responses to acute stress or voluntary activity differed depending on the individual’s physiological state. Stressed fish (i.e. mean heart rates >70 beats min−1) exhibited a bradycardic response to acute stress or activity, whereas fish with mean heart rates <60 beats min−1 instead demonstrated strong tachycardic responses. Remote monitoring of physiological and behavioural variables using bio-loggers can provide unique insights into ‘real-life’ responses of animals, which can largely differ from the responses observed in confined laboratory settings.

Two Nineteenth-Century Catholic Poets: Gerard Manley Hopkins and Aubrey de Vere

202 Studies • volume 106 • number 422 Two Nineteenth-Century Catholic Poets: Gerard Manley Hopkins and Aubrey de Vere Patrick Samway SJ Gerard Manley Hopkins clearly enjoyed both the language of play and the play of language. The linguistic arabesques and interlocking configuration of images in ‘Dun’s Scotus’s Oxford’– such as ‘Cuckoo-echoing, bell-swarmed, lark-charmèd, rook-rackèd, river-rounded’ – are, to cite but one example of such language, traps for poetic meditation. Who among us can easily put his or her arms around this language, to find epistemological connections that are grounded metaphysically in things as they are or things as we know them? Thus, from one perspective, Hopkins could easily become the poster boy for deconstructionism, which indeed treats the ‘play’ side of language – as, all too often, deconstructionists decree that poetry, or novels or any literary texts, for that matter, consist of a series of words without any verifiable ‘meaning’ or ‘centre’ as something, in my mind, antithetical to a Christian world view where Christ is the Verbum or the Logos, and therefore the content of all words. He is the Alpha and Omega of language, of all possible words in all languages, and thus the originator and receptor of all that can be thought or expressed in language. From a study of Duns Scotus, and other philosophers and theologians, Hopkins directed his readers toward words and concepts that allowed them to approach an understanding of his linguistic playfulness, words such as ‘inscape’ and ‘instress’. In this way he assists his reader in coping with the unified complex of characteristics that give each thing its uniqueness and that differentiates it from other things. Studying the ‘inscape’ of a thing shows us why God created it. ‘Each mortal thing does one thing and the same: / . . myself it speaks and spells, / Crying Whát I dó is me: for that I came’. By ‘instress’, he refers to the force of being that holds the inscape together or carries it wholly into the mind of the beholder. Inscape, a word well-beloved to readers of Hopkins’s poetry, likewise can evoke other words, such as landscape, Patrick Samway SJ Studies • volume 106 • number 422 203 which Hopkins uses in ‘Penmaen Pool’, ‘Pied Beauty’ and ‘Ribblesdale’, and/or by extension, at least to my way of thinking, mindscape, which he does not use. Unfortunately, inscape, a word comparable to the French word instase, does not have an opposite, such as outscape, or as the French might say, exstase. Were we to use these new critical words, instase and exstase, in discussing Hopkins, our aim should not be of layering definitions, but of highlighting the originality of Hopkins’s contribution to the poetic enterprise. Thus we might note that in ‘Spring’Hopkins would have us be cognizant of weeds in wheels, thrush’s eggs, a glassy peartree, racing lambs and whatever has existed within the earth’s sweet being from the beginning. Instase looks within a being or within Being itself. ‘Pied Beauty’, to cite another instance, presents skies as the colour of the hides on brinded cows and of the skins of swimming trout, or the design of finches’wings and the shapes of plotted and pieced landscapes, all within a world that reveals the unchangeable beauty of God, who, though one, embodies what the world contains, or else how could he have created it? Instase, the search for what is within, goes beyond itself to lead to exstase, an overwhelming acceptance of the completely Other, in this case of God the Creator and Redeemer. Yet, whatever language one opts for should ultimately give more and more accessibility to Hopkins’s poetry and allow it to take on the clarity one witnesses in seeing the negative of a film develop before our eyes into a well delineated picture. The critical language one adopts is but one way of entering into important considerations of Hopkins’s originality, which can, of course, incorporate valuable literary theory. Yet, there is another way of gauging such originality, that is, by comparing Hopkins with other Catholic poets of his day and their play of language, the conceits that give distinction to their...

Boundary layer control by a fish: Unsteady laminar boundary layers of rainbow trout swimming in turbulent flows.

ABSTRACTThe boundary layers of rainbow trout, Oncorhynchus mykiss [0.231±0.016 m total body length (L) (mean±s.d.); N=6], swimming at 1.6±0.09 L s−1 (N=6) in an experimental flow channel (Reynolds number, Re=4×105) with medium turbulence (5.6% intensity) were examined using the particle image velocimetry technique. The tangential flow velocity distributions in the pectoral and pelvic surface regions (arc length from the rostrum, lx=71±8 mm, N=3, and lx=110±13 mm, N=4, respectively) were approximated by a laminar boundary layer model, the Falkner−Skan equation. The flow regime over the pectoral and pelvic surfaces was regarded as a laminar flow, which could create less skin-friction drag than would be the case with turbulent flow. Flow separation was postponed until vortex shedding occurred over the posterior surface (lx=163±22 mm, N=3). The ratio of the body-wave velocity to the swimming speed was in the order of 1.2. This was consistent with the condition of the boundary layer laminarization that had been confirmed earlier using a mechanical model. These findings suggest an energy-efficient swimming strategy for rainbow trout in a turbulent environment.

Effects of temperature on feed intake and plasma chemistry after exhaustive exercise in triploid brown trout (Salmo trutta L)

The physiological effect of temperature on feed intake and haematological parameters after exhaustive swimming in diploid and triploid brown trout (Salmo trutta) was investigated. Trout were exposed to an incremental temperature challenge (2 °C/day) from ambient (6 °C) to either 10 or 19 °C. Feed intake profiles did not differ between ploidy at 10 °C; however, triploids had a significantly higher total feed intake at 19 °C. After 24 days, each temperature–ploidy group was exposed to exhaustive swimming for 10 min. The haematological response differed between ploidy, with the magnitude of the response affected by temperature and ploidy. Post-exercise, acid–base and ionic differences were observed. Plasma lactate increased significantly from rest for both temperature and ploidy groups, but glucose increased significantly at higher temperature. Post-exercise, triploids at 19 °C had significantly higher osmolality and cholesterol than diploids, but differences were resumed within 4 h. Elevated alkaline phosphatase (ALP) and aspartate aminotransferase (AST) in fish at higher temperature suggested greater tissue damage; however, both ploidy responded similarly. Despite no significant differences in deformity prevalence, the type and location of deformities observed differed between ploidy (decreased intervertebral space with higher prevalence in tail area and fin regions for diploids, while vertebral compression, fusion in cranial and caudal trunks for triploids). These results suggest triploids have greater appetite than diploids at elevated temperature and that triploids suffer similar blood disturbances after exercise as diploids. These findings have implications for the management of freshwater ecosystems and suggest that stocking triploid brown trout may offer an alternative to diploid brown trout.

SWIMMING AGAINST THE FLOW: A ROBOTICS SIMULATION FRAMEWORK

Fish in nature take advantage of some types of turbulence and even generate it to swim with a minimum expenditure of energy. This is the case observed in rainbow trout swimming against the flow in well patterned turbulence phenomenon called Karman Street. Robotic and Multiphysics simulators do not include the possibility of this sort of turbulent flow in interaction with the robot body, to train controllers. Therefore, to better understand how to design a robot that takes advantage of the turbulence, we have developed a simulation framework based on rigid body dynamics software (Webots) and a physics plugin. This plugin has been developed based on a generalized abstraction in the useful area of Karman vortex streets. This framework allows the simulation of user designed robots and their controller interaction with the environment.

Refuging rainbow trout selectively exploit flows behind tandem cylinders.

Fishes may exploit environmental vortices to save in the cost of locomotion. Previous work has investigated fish refuging behind a single cylinder in current, a behavior termed the Kármán gait. However, current-swept habitats often contain aggregations of physical objects, and it is unclear how the complex hydrodynamics shed from multiple structures affect refuging in fish. To begin to address this, we investigated how the flow fields produced by two D-shaped cylinders arranged in tandem affect the ability of rainbow trout (Oncorhynchus mykiss) to Kármán gait. We altered the spacing of the two cylinders from l/D of 0.7 to 2.7 (where l=downstream spacing of cylinders and D=cylinder diameter) and recorded the kinematics of trout swimming behind the cylinders with high-speed video at Re=10,000-55,000. Digital particle image velocimetry showed that increasing l/D decreased the strength of the vortex street by an average of 53% and decreased the frequency that vortices were shed by ∼20% for all speeds. Trout were able to Kármán gait behind all cylinder treatments despite these differences in the downstream wake; however, they Kármán gaited over twice as often behind closely spaced cylinders (l/D=0.7, 1.1, and 1.5). Computational fluid dynamics simulations show that when cylinders are widely spaced, the upstream cylinder generates a vortex street that interacts destructively with the downstream cylinder, producing weaker, more widely spaced and less-organized vortices that discourage Kármán gaiting. These findings are poised to help predict when fish may seek refuge in natural habitats based on the position and arrangement of stationary objects.

Wake-induced ‘slaloming’ response explains exquisite sensitivity of seal whisker-like sensors

Blindfolded harbour seals are able to use their uniquely shaped whiskers to track vortex wakes left by moving animals and identify objects that passed by 30 s earlier, an impressive feat, as the flow features have velocities as low as$1~\text{mm}~\text{s}^{-1}$. The seals sense while swimming, hence their whiskers are sensitive enough to detect small-scale changes in the flow, while rejecting self-generated flow noise. Here we identify and illustrate a novel flow mechanism, causing a large-amplitude ‘slaloming’ whisker response, which allows artificial whiskers with the identical unique undulatory geometry as those of the harbour seal to detect the features of minute flow fluctuations when placed within wakes. Whereas in open water the whisker responds with very low-amplitude vibration, once within a wake, it oscillates with large amplitude and, importantly, its response frequency coincides with the Strouhal frequency of the upstream cylinder, making the detection of an upstream wake and an estimation of the size and shape of the wake-generating body possible. This mechanism has some similarities with the flow mechanisms observed in actively controlled propulsive foils within upstream wakes and trout swimming behind bluff cylinders in a stream, but there are also differences caused by the unique whisker morphology, which enables it to respond passively and within a much wider parametric range.

Brain glycogen supercompensation after different conditions of induced hypoglycemia and sustained swimming in rainbow trout (Oncorhynchus mykiss)

Brain glycogen is depleted when used as an emergency energy substrate. In mammals, brain glycogen levels rebound to higher than normal levels after a hypoglycemic episode and a few hours after refeeding or administration of glucose. This phenomenon is called glycogen supercompensation. However, this mechanism has not been investigated in lower vertebrates. The aim of this study was therefore to determine whether brain glycogen supercompensation occurs in the rainbow trout brain. For this purpose, short-term brain glucose and glycogen contents were determined in rainbow trout after being subjected to the following experimental conditions: i) a 5-day or 10-day fasting period and refeeding; ii) a single injection of insulin (4mgkg−1) and refeeding; and iii) sustained swimming and injection of glucose (500mgkg−1). Food deprivation during the fasting periods and insulin administration both induced a decrease in glucose and glycogen levels in the brain. However, only refeeding after 10days of fasting significantly increased the brain glycogen content above control levels, in a clear short-term supercompensation response. Unlike in mammals, prolonged exercise did not alter brain glucose or glycogen levels. Furthermore, brain glycogen supercompensation was not observed after glucose administration in fish undergoing sustained swimming. To our knowledge, this is the first study providing direct experimental evidence for the existence of a short-term glycogen supercompensation response in a teleost brain, although the response was only detectable after prolonged fasting.

No evidence for a bioenergetic advantage from forced swimming in rainbow trout under a restrictive feeding regime

Sustained swimming at moderate speeds is considered beneficial in terms of the productive performance of salmonids, but the causative mechanisms have yet to be unequivocally established. In the present study, the effects of moderate exercise on the bioenergetics of rainbow trout were assessed during a 15 week growth experiment, in which fish were reared at three different current speeds: 1 BL s−1, 0.5 BL s−1 and still water (≈ 0 BL s−1). Randomly selected groups of 100 fish were distributed among twelve 600 L tanks and maintained on a restricted diet regime. Specific growth rate (SGR) and feed conversion ratio (FCR) were calculated from weight and length measurements every 3 weeks. Routine metabolic rate (RMR) was measured every hour as rate of oxygen consumption in the tanks, and was positively correlated with swimming speed. Total ammonia nitrogen (TAN) excretion rates showed a tendency to decrease with increasing swimming speeds, yet neither they nor the resulting nitrogen quotients (NQ) indicated that swimming significantly reduced the fraction of dietary protein used to fuel metabolism. Energetic budgets revealed a positive correlation between energy expenditure and the current speed at which fish were reared, fish that were forced to swim and were fed restrictively consequentially had poorer growth and feed utilization. The results show that for rainbow trout, water current can negatively affect growth despite promoting minor positive changes in substrate utilization. We hypothesize that this may be the result of either a limited dietary energy supply from diet restriction being insufficient for both covering the extra costs of swimming and supporting enhanced growth.

Exogenous lactate supply affects lactate kinetics of rainbow trout, not swimming performance.

Intense swimming causes circulatory lactate accumulation in rainbow trout because lactate disposal (Rd) is not stimulated as strongly as lactate appearance (Ra). This mismatch suggests that maximal Rd is limited by tissue capacity to metabolize lactate. This study uses exogenous lactate to investigate what constrains maximal Rd and minimal Ra. Our goals were to determine how exogenous lactate affects: 1) Ra and Rd of lactate under baseline conditions or during graded swimming, and 2) exercise performance (critical swimming speed, Ucrit) and energetics (cost of transport, COT). Results show that exogenous lactate allows swimming trout to boost maximal Rd lactate by 40% and reach impressive rates of 56 μmol·kg(-1)·min(-1). This shows that the metabolic capacity of tissues for lactate disposal is not responsible for setting the highest Rd normally observed after intense swimming. Baseline endogenous Ra (resting in normoxic water) is not significantly reduced by exogenous lactate supply. Therefore, trout have an obligatory need to produce lactate, either as a fuel for oxidative tissues and/or from organs relying on glycolysis. Exogenous lactate does not affect Ucrit or COT, probably because it acts as a substitute for glucose and lipids rather than extra fuel. We conclude that the observed 40% increase in Rd lactate is made possible by accelerating lactate entry into oxidative tissues via monocarboxylate transporters (MCTs). This observation together with the weak expression of MCTs and the phenomenon of white muscle lactate retention show that lactate metabolism of rainbow trout is significantly constrained by transmembrane transport.

Swimming fish stick to same Strouhal number

Fish swimming at a certain speed appear to adopt a narrow range of swimming styles instinctively. Robert Nudds and colleagues from the University of Manchester, UK, explain that fish seem to adapt their undulations so that the product of their tail beat frequency and the maximum displacement of the tail tip when divided by the fish's forward velocity almost always converges to a number ranging from 0.2 to 0.4, known as the Strouhal number. This means that, at a certain swimming speed, if the fish beats its tail faster it compensates by dropping the amplitude of the tail's undulation, or if the tail beats become broader, they slow down. The same phenomenon, where movements are conserved to a narrow range of Strouhal numbers during locomotion, has also been found in bird flight, leading scientists to conclude that animal movements have been tightly constrained by evolution to maximise propulsive efficiency. However, Nudds points out that none of the observations that have directed scientists to this conclusion had been collected systematically, so Nudds, Emma John, Adam Keen and Holly Shiels decided to resolve the problem by investigating how trout adjust their swimming styles in warmer waters (p. 2244).The team explains that ectothermic fish are susceptible to the environmental temperature, so when the temperature rises, the fish's metabolic rate rises also, reducing the amount of energy that they can invest in swimming at higher speeds and affecting how they beat their tails. With that in mind, the scientists filmed young trout swimming at speeds from 0.28 to 1.11 m s−1 in water at temperatures of 11 and 20°C while recording the fish's tail beat frequency and amplitude.After painstakingly analysing hours of swimming data to calculate the fish's Strouhal number at different speeds and temperatures, the team could see that the fish maintained the same Strouhal number when swimming at both the low and high temperatures at any given speed. The hotter fish beat their tails faster than the cooler fish, but they compensated by reducing the amplitude of each tail beat to maintain a constant Strouhal number at that speed. ‘This is the first experimental evidence for an apparent adherence to a preferred (perhaps optimum) Strouhal number for an animal using oscillatory propulsion’, the team says.However, when they calculated how the fish's Strouhal number varied as the animals speeded up, they saw that the number gradually increased from 0.19 at the lowest speeds to 0.22 at the highest. Although the fish continued beating their tails at the same rate, they increased their speed by sweeping the tail further. The team suggests that the fish's apparent inability to increase their tail beat frequency indicates that their muscle contraction frequency might be finely tuned to a very narrow range at any given temperature. And when they measured the metabolic rate of the fish, although the animals' basal metabolic rate increased with temperature, they did not have to increase the amount of effort they put into fast swimming at higher temperatures. The team suggests that small increases in water temperature may even benefit trout swimming performance. They conclude by saying, ‘Future predictions of changes to water temperature of lakes and rivers are only 5–10°C over the next 100 years and it appears that rainbow trout, at least, can cope with this easily in terms of swimming biomechanics.’

Altered burst swimming in rainbow trout Oncorhynchus mykiss exposed to natural and synthetic oestrogens.

Juvenile rainbow trout Oncorhynchus mykiss were exposed to two concentrations each of 17β-oestradiol (E2; natural oestrogen hormone) or 17α-ethinyl oestradiol (EE2; a potent synthetic oestrogen hormone) to evaluate their potential effects on burst-swimming performance. In each of six successive burst-swimming assays, burst-swimming speed (Uburst ) was lower in fish exposed to 0.5 and 1 µg l(-1) E2 and EE2 for four days compared with control fish. A practice swim (2 days prior to exposure initiation) in control fish elevated initial Uburst values, but this training effect was not evident in the 1 µg l(-1) EE2-exposed fish. Several potential oestrogen-mediated mechanisms for Uburst reductions were investigated, including effects on metabolic products, osmoregulation and blood oxygen-carrying capacity. Prior to burst-swimming trials, fish exposed to E2 and EE2 for 4 days had significantly reduced erythrocyte numbers and lower plasma glucose concentrations. After six repeated burst-swimming trials, plasma glucose, lactate and creatinine concentrations were not significantly different among treatment groups; however, plasma Cl(-) concentrations were significantly reduced in E2- and EE2-treated fish. In summary, E2 and EE2 exposure altered oxygen-carrying capacity ([erythrocytes]) and an osmoregulatory-related variable ([Cl(-) ]), effects that may underlie reductions in burst-swimming speed, which will have implications for fish performance in the wild.

Measuring gill paracellular permeability with polyethelene glycol-4000 in freely swimming trout: proof of principle

The influence of swimming activity on gill paracellular permeability has not been measured previously in fishes. We critically assessed the use of tritium-labeled polyethylene glycol ([(3)H]PEG-4000) for this purpose, a substance that is also a classic marker for extracellular fluid volume, glomerular filtration rate and drinking rate. Tests (8 h) on resting freshwater trout showed that when measuring [(3)H]PEG-4000 clearance from the plasma in the efflux direction, correction for a large excretion via glomerular filtration was essential, necessitating urinary catheterization. When measuring [(3)H]PEG-4000 clearance from the water in the influx direction, correction for a significant uptake by drinking was essential, necessitating terminal gut removal, whereas glomerular filtration losses were minimal. After correction for these alternate routes of loss and uptake, [(3)H]PEG-4000 clearance rates by efflux from the plasma and by influx from the water were identical, showing that gill paracellular permeability is not rectified, and can be measured in either direction. The influx technique with terminal gut removal was used to assess gill paracellular permeability in trout without urinary catheters freely swimming at 1.2 body lengths s(-1) for 8 h. Branchial [(3)H]PEG-4000 clearance rate (by influx from the water) increased significantly by ~80% in accord with a similar measured increase in O2 consumption rate. Thus in trout, gill paracellular permeability does increase during exercise, in accord with the traditional concept of the osmorespiratory compromise.

A Flexible Fin with Bio-Inspired Stiffness Profile and Geometry

Biological evidence suggests that fish use mostly anterior muscles for steady swimming while the caudal part of the body is passive and, acting as a carrier of energy, transfers the momentum to the surrounding water. Inspired by those findings we hypothesize that certain swimming patterns can be achieved without copying the distributed actuation mechanism of fish but rather using a single actuator at the anterior part to create the travelling wave. To test the hypothesis a pitching flexible fin made of silicone rubber and silicone foam was designed by copying the stiffness distribution profile and geometry of a rainbow trout. The kinematics of the fin was compared to that of a steadily swimming trout. Fin's propulsive wave length and tail-beat amplitude were determined while it was actuated by a single servo motor. Results showed that the propulsive wave length and tail-beat amplitude of a steadily swimming 50 cm rainbow trout was achieved with our biomimetic fin while stimulated using certain actuation parameters (frequency 2.31 Hz and amplitude 6.6 degrees). The study concluded that fish-like swimming can be achieved by mimicking the stiffness and geometry of a rainbow trout and disregarding the details of the actuation mechanism.

Entraining in trout: a behavioural and hydrodynamic analysis

Rheophilic fish commonly experience unsteady flows and hydrodynamic perturbations. Instead of avoiding turbulent zones though, rheophilic fish often seek out these zones for station holding. A behaviour associated with station holding in running water is called entraining. We investigated the entraining behaviour of rainbow trout swimming in the wake of a D-shaped cylinder or sideways of a semi-infinite flat plate displaying a rounded leading edge. Entraining trout moved into specific positions close to and sideways of the submerged objects, where they often maintained their position without corrective body and/or fin motions. To identify the hydrodynamic mechanism of entraining, the flow characteristics around an artificial trout placed at the position preferred by entraining trout were analysed. Numerical simulations of the 3-D unsteady flow field were performed to obtain the unsteady pressure forces. Our results suggest that entraining trout minimise their energy expenditure during station holding by tilting their body into the mean flow direction at an angle, where the resulting lift force and wake suction force cancel out the drag. Small motions of the caudal and/or pectoral fins provide an efficient way to correct the angle, such that an equilibrium is even reached in case of unsteadiness imposed by the wake of an object.