Sunflowers are grown in various countries throughout the world and used for cooking oil, biodiesel, animal feed and as ornamental plants. Spodoptera frugiperda (JE Smith) is a polyphagous insect species that is native to tropical America and that has spread rapidly around the world. The purpose of the current study was to evaluate resistance of sunflower genotypes to S. frugiperda . This was accomplished by assessing antibiosis and antixenosis in 25 sunflower genotypes. Were evaluated: free and no-choice attractiveness test, larval stage: period and viability of the larval stage and weight of caterpillars at ten days of age; pre-pupa stage: period and viability; pupal stage: period, weight at 24 hours of age and viability; complete cycle: period and viability; adult stage: daily longevity assessments and sex ratio; nutritional parameters The BRS 55 and BRS 64 genotypes showed antixenosis resistance in choice and no-choice tests. Biological and nutritional measures demonstrated that the BRS 323, BRS 56, BRS 65 genotypes were unfavorable to S. frugiperda development. These genotypes showed moderate resistance and could be used by sunflower growers as a way of controling S. frugiperda.