Trolox Equivalent Antioxidant Capacity Research Articles

Exploring the Addition of Mango Peel in Functional Semolina Sourdough Bread Production for Sustainable Bio-Reuse

Mango, a tropical fruit celebrated for its delightful fragrance and high nutritional value, generates significant waste during processing, with approximately 35–60% of the fruit being discarded. However, this waste contains valuable components, such as fibre, carotenoids, polyphenols, and other bioactive compounds. In an effort to repurpose mango peel, this study dehydrated it to create mango peel powder (MPP), which was then incorporated into sourdough bread to produce functional breads with enhanced nutritional value. Semolina was replaced with MPP at levels of 5% (MPP-5) and 10% (MPP-10) (w/w). After dehydration, the mango peel had a yield of 22%, and the procedure used did not cause any organoleptic changes. The bread fermentation process was conducted at 30 °C for 8 h. During dough fermentation, the pH was monitored, showing a value of 4.14 ± 0.02 in the MPP-10 dough. Overall, the MPP-10 bread received a higher score (6.51) than the control (CTR) bread (5.6) and the MPP-5 bread (6.11). The total phenolic content of the fortified breads ranged from 44.760 to 98.931 mg gallic acid equivalents (GAE)/g, and the antiradical activity ranged from 15.213 to 29.461 mmol trolox equivalent antioxidant activity (TEAC)/100 g, depending on the percentage of enrichment.

Analysis of Wormwood (Artemisia absinthium L.) teas

Artemisia absinthium L., commonly known as wormwood, is a medicinal herb with deep roots in traditional medicine. Wormwood possesses numerous healing properties, including positive effects on the liver, bladder, stomach and intestines. It improves digestion, has the antidiabetic effect and exhibits antioxidant, anti-inflammatory and anticancer properties. Although wormwood can be used in various forms, the simplest and most accessible method is wormwood tea, prepared from dried raw material.In this study, the antioxidant properties and total phenol content of wormwood teas prepared using different methods and raw materials from different manufacturers were investigated. The DPPH radical scavenging activity, expressed in Trolox equivalent antioxidant capacity, ranged from 457 to 623 mg/l, while the total phenol content, expressed in gallic acid equivalents, ranged from 112 to 224 mg/l. The findings suggest that the antioxidant properties of wormwood tea are largely influenced by phenolic compounds. It was found that teas made from wormwood leaves exhibit higher antioxidant activity and phenolic content compared to those made from wormwood stems. The main volatile components of wormwood teas were identified as β-thujone and trans-sabinyl acetate, with β-thujone content in wormwood teas ranging from 36 to 79 mg/l. Additionally, the thujone content decreases when the tea is brewed.

The relationships between dietary inflammatory and antioxidant index with inflammatory markers in children with acute lymphocytic leukemia

Purpose The purpose of this study is to investigate the relationship between dietary total antioxidant capacity (DTAC) and the dietary inflammatory index (DII) with inflammatory status in children recently diagnosed with Acute Lymphoblastic Leukemia (ALL). As the relationship between diet quality scores and inflammation remains uncertain in this population, the authors carried out a hospital-based cross-sectional study. Design/methodology/approach The study used a cross-sectional design involving 54 children recently diagnosed with ALL. Dietary data was collected using a 147-item semi-quantitative food frequency questionnaire developed for the Tehran Lipid and Glucose Study. Inflammatory status was evaluated using various indicators like C-reactive protein (CRP), neutrophil-to-lymphocyte ratio, platelet-to-lymphocyte ratio (PLR), monocyte-to-lymphocyte ratio (MLR), Glasgow prognostic score (GPS) and Complete blood count-based inflammatory score. Findings The authors found no significant relationship between DII with inflammatory indices and length of hospitalization in patients. However, a significant inverse relationship was observed between the antioxidant indices ferric-reducing ability of plasma, total reactive antioxidant potential and Trolox equivalent antioxidant capacity with the inflammatory index MLR, respectively (ß: −0.19, p = 0.001), (ß: −0.42, p = 0.02), (ß: −0.53, p = 0.005). Also, a significant inverse relationship between FRAP and CRP was observed in the crude model (ß: −0.13, p = 0.05). FRAP was also inversely correlated with GPS and PLR, respectively (ß: −0.08, p = 0.02), (ß: −0.26, p = 0.03). No additional significant links were discovered between food scores and the outcomes studied. Originality/value This study found no link between DII with inflammatory markers or hospitalization duration in children with ALL. However, the authors did observe a noteworthy inverse relationship between DTAC and certain inflammatory markers like MLR. To achieve more dependable findings, further research in this area is necessary.

Quince (Cydonia oblonga Mill.) Waste By-Product Characterization as a Potential Functional Ingredient

Currently, the production of waste in the food industry is increasing, which is a serious problem. However, most of these residues, especially those derived from fruits and vegetables, have great unknown properties that are not used. The main objective of this article is the analysis and characterization of the waste from quince after its processing to observe its properties and its potential use in different industries as a functional ingredient, thus favoring the circular economy and sustainability. Quince by-product nutritional parameters such as proteins, fibers, sugars, vitamins, and minerals were analyzed. Also, the antioxidant capacity was measured by various methods: 1,1-diphenyl-2-picrylhydrazyl radical scavenging activity (DPPH), antioxidant capacity in Trolox equivalent antioxidant capacity (TEAC/ABTS), and total polyphenol content (TPC). Finally, the antimicrobial capacity against different postharvest-pathogenic fungi was measured in direct sample and extract. The nutritional results showed a nutritional profile rich in soluble and insoluble fiber, potassium, calcium, and magnesium, and low in fat. The antioxidant results from the extract showed significant levels of phenols and higher antioxidant capacity from the extracted sample. No positive results were found in the antimicrobial capacity study. Quince by-products could be a potential ingredient in the industry due to their nutritional composition and antioxidant content.

Protective Effects of Purple Corn (Zea mays L.) Byproduct Extract on Blue Light-Induced Retinal Damage in A2E-Accumulated ARPE-19 Cells.

This study investigated the antioxidative characteristics of Zea mays L. purple corn cob and husk extract (PCHE) and its potential protective effects against blue light (BL)-induced damage in N-retinylidene-N-retinylethanolamine (A2E)-accumulated ARPE-19 retinal pigment epithelial cells. PCHE had a 2,2-diphenyl-1-picrylhydrazyl radical-scavenging capacity and Trolox equivalent antioxidant capacity of 1.28±0.43 mM Trolox equivalents (TE)/g and 2,545.41±34.13 mM TE/g, respectively. Total content of anthocyanins, polyphenols, and flavonoids in the PCHE was 11.13±0.10 mg cyanidin-3-glucoside equivalents/100 g, 227.90±7.38 mg gallic acid equivalents/g, and 117.75±2.46 mg catechin equivalents/g, respectively. PCHE suppressed the accumulation of A2E and the photooxidation caused by BL in a dose-dependent manner. After initial treatment with 25 µM/mL A2E and BL, ARPE-19 cells showed increased cell viability following additional treatment with 15 µg/mL PCHE while the expression of the p62 sequestosome 1 decreased, whereas that of heme oxygenase-1 protein increased compared with that in cells without PCHE treatment. This suggests that PCHE may slow the autophagy induced by BL exposure in A2E-accumulated retinal cells and protect them against oxidative stress.

The principal component analysis of antioxidant activities of Songgak, a traditional herbal drink in West Nusa Tenggara, Indonesia

Songgak is a traditional herbal tea of the Sasak Tribe in Lombok Island, Indonesia, formulated to strengthen people's stamina. Songgak is composed of seven kinds of roasted spices: Piper nigrum L., Coriandrum sativum L., Piper retrofractum Vahl, Myristica fragrans Houtt, Syzygium aromaticum, Elaeocarpus grandiflorus, and Helicteres isora. This study aimed to assess the benefits of Songgak and its constituent spices by evaluating their antioxidant activities, total phenolic content (TPC) and flavonoid content (TPC). Antioxidant activity tests were performed on the water extract of Songgak, ethanolic extract of Songgak, and ethanolic extract of seven spices of Songgak including 2,2′- diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging, Trolox equivalent antioxidant capacity (TEAC), and ferric reducing antioxidant power (FRAP) assay. The antioxidant activity using DPPH method showed that the water extract of Songgak has a strong categorisation of antioxidant activity with DPPH IC50 value of 63.78±4.29 µg/mL. The constituent species of Songgak with the strongest antioxidant activity was S. aromaticum. Total phenolic content (TPC) and total flavonoid content (TFC) of Songgak tea were 26.85±4.29 mg GAE/g and 6.99±0.29 mg RE/gram, respectively. The correlation between TPC, TFC, and antioxidant activities was determined using principal component analysis (PCA). The loading plot showed that TPC and TFC were strongly correlated with the antioxidant activity (FRAP method) of Songgak tea and its constituent spices. These results revealed that the presence of flavonoid and phenolic compounds in the seven herbal medicinal plants were responsible for the antioxidant properties of Songgak as a healthy herbal drink. Therefore, consuming Songgak every day can be beneficial for preventing various diseases involving free radicals.

The Antioxidant Activity of Atomized Extracts of the Leaves and Stems of Cnidoscolus diacanthus (Pax & K. Hoffm.) J.F. Macbr. from Peru and Their Effect on Sex Hormone Levels in Rats

In this research, we aimed to determine the antioxidant activity of an atomized extract of Cnidoscolus diacanthus (Pax & K. Hoffm.) J.F. Macbr., known in Peru as “huanarpo hembra”, and its effect on sex hormone levels. Its phytochemical profile was determined using liquid chromatography–mass spectrometry (LC–MS), while its total phenol content (TPC) and total flavonoids (TFs) were determined using the Folin–Ciocalteu method and the aluminum chloride method. Its antioxidant activity was determined using 2,2-diphenyl-1-picrylhydrazyl (DPPH), the radical 2,2-azino-bis-3-ethylbenzthiazolin-6 sulfonic acid (ABTS), and ferric-reducing antioxidant power (FRAP). The biological activity of C. diacanthus and its effect on sexual hormones were determined in Holtzman rats of both sexes. Phytochemical analysis revealed the presence of flavonoids and phenolic compounds in its leaves and stems, mainly rutin, quercetin, chlorogenic acid, and genistein. However, the stem extract contained higher total phenol (464.38 ± 4.40 GAE/g) and flavonoid (369.17 ± 3.16 mg QE/g of extract) contents than the leaf extract (212.38 ± 3.19 mg GAE/g and 121.49 ± 2.69 mg QE/g). For DPPH, ABTS, and FRAP, the Trolox-equivalent antioxidant capacity (TEAC) was 597.20 ± 5.40 µmol/g, 452.67 ± 5.76 µmol/g, and 535.91 ± 1.56 µmol/g, respectively, for the stems, while for the leaves, it was 462.39 ± 3.99 µmol/g, 202.32 ± 5.20 µmol/g, and 198.13 ± 1.44 µmol/g, respectively. In terms of the values for hormonal levels, at a dose of 100 mg/kg of the extract, testosterone levels of 1.430 ng/mL (with the leaf extract) and 1.433 ng/mL (with the stem extract), respectively, were found in the male rats. Regarding estradiol levels, in the female rats, these were 10.425 ng/mL (leaf extract) and 8.775 ng/mL (stem extract), while their levels of luteinizing hormone were 0.320 mIU/mL (leaf extract) and 0.273 mIU/mL (stem extract). For the follicle-stimulating hormone, levels of 0.858 mIU/mL (leaf extract) and 0.840 mIU/mL (stem extract) were found in the female rats, and levels of 0.220 mIU/mL (leaf extract) and 0.200 mIU/mL (stem extract) were found in the male rats. It is concluded that the C. diacanthus stem extract had a greater antioxidant capacity than the leaf extract, while both extracts had a superior effect on the sex hormone levels in the female rats compared to the male rats.

Phytochemical, antioxidant and antibacterial studies of extracts and chromatographic fractions of <i>Gmelina arborea</i> Roxb (Lamiaceae)

Antimicrobial resistance and oxidative stress are increasing and researchers are being encouraged to search the natural plant products, due to their popular usage in ethno-medicine, for alternative source of antimicrobial and antioxidant compounds. Gmelina arborea used in West-Africa and Ayurveda folkloric medicine to cure several diseases was therefore investigated for phytochemical, antioxidant and antimicrobial activities. Extracts and chromatographic fractions of the root-bark tested for antibacterial activity with the MIC determined on seven selected bacteria using agar dilution method. The antioxidant capacity of the crude extracts was determined by six (6) methods: total flavonoid content, total phenolic content, ferric reducing power, total antioxidant capacity, Trolox equivalent antioxidant capacity and DPPH (2,2-diphenyl-1-picryhydrazyl) properties. Phytochemical screening indicated the presence of saponins, tannins, flavonoids, terpenoids anthraquinones, phenols and alkaloids. The methanol and ethyl-acetate extracts of the plant showed good antibacterial activities (18 mm inhibitory zones and MIC and MBC 12.5–100 mg/mL) against P. aeruginosa ATCC 27853 and S. aureus ATCC 6571. Antioxidant study of the extracts revealed that both ethyl acetate and methanol extracts have good antioxidant capacity comparable to that of ascorbic acid standard. Therefore, Gmelina arborea could prove a valuable source of developing new antimicrobial and antioxidant compounds for therapeutic uses.

Deep eutectic solvent strategy for green extraction of chlorogenic acid from sea buckthorn: optimization and sustainability

BackgroundSea buckthorn (Hippophae rhamnoides), a deciduous species plant, is widely distributed around the globe, and native to the cold-temperate regions of Europe and Asia. This medicinal herb contains several bioactive constituents including chlorogenic acid. The conventional methods used for the extraction of phenolic antioxidants from natural herbs often result in low yields, high toxicity, and pose environmental hazards limiting their effectiveness and scalability. Therefore, green extraction techniques using deep eutectic solvents, composed of natural, non-toxic, and biodegradable components were applied for extraction of chlorogenic acid from sea buckthorn weed. Fourteen deep eutectic solvent mixtures were prepared and evaluated for extraction yield of chlorogenic acid. Parameters such as hydrogen bond donor-to-hydrogen bond acceptor ratio, liquid-to-solid ratio, shaking speed, and shaking time were optimized for the best mixture.ResultsThe combination of lactic acid and maltose (1:1) was found to give best extraction yield using response surface methodology. The deep eutectic solvent system under optimum conditions produced 12.2 g/100 g of crude extract sea buckthorn containing 174.7 mg gallic acid equivalents (mg GA)/g) of extract. Moreover, the optimized extract exhibited appreciable radical scavenging capacity (91%), trolox equivalent antioxidant capacity (11.2% of extract), and inhibition of peroxide in linoleic acid (80.6%). High-performance liquid chromatography-based characterization revealed the extracts contained chlorogenic acid (20.1 mg/g of extract) as the major constituent.ConclusionsIn summary, the adoption of DES for the extraction of bioactive phenolic constituents from sea buckthorn offers multiple benefits, including economic efficiency, enhanced extraction performance, and environmental sustainability. The findings of this study not only advance the understanding of DES in phytochemical extraction but also pave the way for broader application of green solvents in the natural products industry. Future research should focus on further optimizing DES formulations and scaling up the extraction process to fully realize the potential of this innovative extraction method in commercial applications.

Phytochemical and Antioxidant Profiling of Chilli (Capsicum annuum L.) Landraces from Hilly Regions of India

The present study was carried out to assess the phytochemical and antioxidant potential of 24 chilli landraces grown in hilly regions of India viz. Himachal Pradesh & Assam and genotypes were evaluated for different phytochemical parameters and antioxidant assays. Highest total sugar (3.43 %), reducing sugar (2.22 %), polyphenolic content (153.85 mg /100g GAE), total carotenoids (101.66 mg/100g), ascorbic acid (12.85 mg/100g) and reducing power assay (70.76 mM/g TE) were found in the genotype ‘Chilli SC-1’, while ‘Chilli CHI-15’ was found superior for total flavonoid content (547.01 mg/100g QE). The genotype, ‘Chilli SC-1’followed ‘Chilli L-2’ were found best owing to their Trolox equivalent antioxidant activity. The above results were also confirmed with spectral studies using FT-IR spectroscopy and genotype ‘Chili SC-1’ was found best in all the concerned parameters as the highest peaks of the respective sample were observed concerning all other samples. Further, principal component analysis indicated that the genotype ‘Chilli SC-1’, ‘Chilli LC-2’, ‘Chilli AC-9’ and ‘Chilli AC-10’ are the major contributors to divergence within different quality traits under study.

A Procedure for the Preparation of Some Natural Dietary Supplements Based on Coenzyme Q10 from Chicken Hearts and Oil Press Cakes

This study proposes a procedure for preparing some natural dietary supplements based on CoQ10 (coenzyme Q10) from lyophilised chicken hearts, respectively, oil press cakes of sunflower, rapeseed, walnut, and pumpkin. It consists of homogenising the powders obtained from these matrices with 2-propanol, succeeded by sonication, overnight maceration, collecting the liquid phase and filtering it under vacuum, removing the solvent by rotary evaporation, eliminating the 2-propanol traces under a nitrogen stream, and, finally, packaging in 10-mL amber glass bottles. Five dietary supplements were prepared using the above-described procedure and kept at room temperature for 9 months to evaluate their storage stability. To this end, CoQ10, TEAC (Trolox equivalent antioxidant capacity), and PV (peroxide value) levels were determined initially and every 3 months of storage. The highest levels of CoQ10 were found in the dietary supplements from lyophilised chicken hearts (18.12 mg/10 mL) and pumpkin press cakes (5.48 mg/10 mL). However, those from rapeseed and walnut press cakes had the highest levels of TEAC (19.23 µmol TE/g and 15.31 µmol TE/g, respectively). Exceeding the maximum permissible level for PV revealed a shelf-life of 3 months for the dietary supplements from pumpkin and walnut press cakes and less than that for the others.

Effects of Fermentation with Kombucha Symbiotic Culture of Bacteria and Yeasts on Antioxidant Activities, Bioactive Compounds and Sensory Indicators of Rhodiola rosea and Salvia miltiorrhiza Beverages.

Kombucha is a well-known fermented beverage traditionally made from black tea infusion. Recent studies have focused on finding alternative materials to create novel kombucha beverages with various health benefits. In this study, we prepared and evaluated two novel kombucha beverages using Rhodiola rosea and Salvia miltiorrhiza as materials. The effects of fermentation with the residue of these plants on the kombucha were also investigated. The antioxidant activities, total phenolic contents, and concentrations of the bioactive compounds of the kombucha beverages were determined by the Trolox equivalent antioxidant capacity test, ferric-reducing antioxidant power test, Folin-Ciocalteu method, and high-performance liquid chromatography, respectively. The results revealed that the kombucha beverages made with Rhodiola rosea and Salvia miltiorrhiza had strong antioxidant capacities and abundant phenolic contents. Additionally, the kombucha fermented with Rhodiola rosea residue had higher FRAP, TEAC and TPC values than that fermented without residue. On the other hand, the Salvia miltiorrhiza kombucha fermented with residue had similar FRAP and TEAC values but lower TPC values compared to that fermented without residue. The correlation analysis showed that gallic acid, salidroside, and tyrosol were responsible for the antioxidant abilities and total phenolic contents of the Rhodiola rosea kombucha, and salvianolic acid A and salvianolic acid B contributed to the antioxidant abilities of the Salvia miltiorrhiza kombucha. Furthermore, the kombucha fermented with Rhodiola rosea residue had the highest sensory scores among the kombucha beverages studied. These findings suggest that Rhodiola rosea and Salvia miltiorrhiza are suitable for making novel kombucha beverages with strong antioxidant abilities and abundant phenolic contents, which can be used in preventing and managing oxidative stress-related diseases.

Evaluation of the growth performance, meat quality, and gut microbiota of broilers fed diets containing walnut green husk extract

This study was conducted to evaluate the effects of walnut green husk extract (WGHe) on the growth performance, meat quality, antioxidative status, gut morphology, and microbiota diversity of broilers. A total of 216 one-day-old broilers were divided into 4 groups, each consisting of 9 replicates (6 birds per replicate) as follows: 1) control group, basal diet; 2) antibiotic group, basal diet supplemented with enduracidin and colistin sulfate; 3) low-dose group, basal diet supplemented with 5.0 g/kg WGHe; and 4) high-dose group, basal diet supplemented with 10.0 g/kg WGHe. The results revealed that the percentage of abdominal fat decreased, and the ratio of the duodenal villus length to crypt depth (V/C), as well as the α-diversity of the ileal microbiota, increased with 10.0 g/kg WGHe supplementation (P < 0.05). The shear force of the breast muscle and plasma malondialdehyde (MDA) concentration decreased, whereas the plasma peroxidase (POD) activity, Trolox equivalent antioxidant capacity (TEAC), and jejunal villus length increased in response to WGHe supplementation (P < 0.05). Compared with the antibiotic diet, the addition of 5.0 g/kg WGHe resulted in a significant increase in the relative abundances of Candidatus Arthromitus, Eubacterium coprostanoligenes, and Ruminococcaceae UCG-014 (P < 0.01). Furthermore, the addition of 10.0 g/kg WGHe increased the relative abundances of Candidatus Arthromitus and Lachnoclostridium, whereas the relative abundance of unidentified Chloroplast decreased (P < 0.05). In conclusion, dietary supplementation with 10.0 g/kg WGHe is advantageous for intestinal health, meat quality, and antioxidant status in broilers, suggesting its potential as a functional additive in poultry production.

Electrospun Poly-ε-Caprolactone Nanofibers Incorporating Keratin Hydrolysates as Innovative Antioxidant Scaffolds.

This manuscript describes the development and characterization of electrospun nanofibers incorporating bioactive hydrolysates obtained from the microbial bioconversion of feathers, a highly available agro-industrial byproduct. The electrospun nanofibers were characterized using different instrumental methods, and their antioxidant properties and toxicological potential were evaluated. Keratin hydrolysates (KHs) produced by Bacillus velezensis P45 were incorporated at 1, 2.5, and 5% (w/w) into poly-ε-caprolactone (PCL; 10 and 15%, w/v solutions) before electrospinning. The obtained nanofibers were between 296 and 363 nm in diameter, showing a string-like morphology and adequate structural continuity. Thermogravimetric analysis showed three weight loss events, with 5% of the mass lost up to 330 °C and 90% from 350 to 450 °C. Infrared spectroscopy showed typical peaks of PCL and amide bands corresponding to keratin peptides. The biological activity was preserved after electrospinning and the hemolytic activity was below 1% as expected for biocompatible materials. In addition, the antioxidant capacity released from the nanofibers was confirmed by DPPH and ABTS radical scavenging activities. The DPPH scavenging activity observed for the nanofibers was greater than 30% after 24 h of incubation, ranging from 845 to 1080 µM TEAC (Trolox equivalent antioxidant capacity). The antioxidant activity for the ABTS radical assay was 44.19, 49.61, and 56.21% (corresponding to 972.0, 1153.3, and 1228.7 µM TEAC) for nanofibers made using 15% PCL with 1, 2.5, and 5% KH, respectively. These nanostructures may represent interesting antioxidant biocompatible materials for various pharmaceutical applications, including wound dressings, topical drug delivery, cosmetics, and packaging.

Polyunsaturated fatty acid status and markers of oxidative stress and inflammation across the lifespan: A cross-sectional study in a cohort with long-lived individuals

Polyunsaturated fatty acids (PUFA) are known to have a regulatory effect on oxidative and inflammatory processes. This study aimed to identify the relationship between blood PUFA status and circulatory markers of oxidative stress and inflammation in a cohort of 172 subjects. The population was divided by sex and into three age groups: adults (18–64 years old, n = 69), older adults (65–89 years old, n = 54), and long-lived individuals (LLIs, 90–111 years old, n = 49). Whole blood PUFA content was quantified using gas chromatography. Additionally, serum levels of C-reactive protein (CRP), paraoxonase (PON), Trolox equivalent antioxidant capacity (TEAC), and malondialdehyde (MDA) were measured. Our results showed that a higher omega-3 (n-3) index in adult females was a predictor of lower MDA concentrations (p = 0.038). Conversely, total n-3 PUFA and total n-6 PUFA were positively related to MDA values among older adult females and LLI men (p < 0.05), while total n-6 PUFA was inversely correlated with MDA levels in LLI females (p < 0.05). Interestingly, increased concentrations of total n-3 PUFA and n-3 index were positively correlated with higher TEAC values in LLI men (p = 0.007), while the arachidonic acid (AA)/eicosapentaenoic acid (EPA) ratio was inversely correlated with TEAC values among LLI females (p = 0.006). These findings suggest that cellular antioxidant capacity is inversely correlated with changes in the AA/EPA ratio in long-lived females, whereas n-3 PUFA may enhance blood antioxidant capacity in long-lived men. Overall, our study highlights the complex, sex-specific interactions between PUFA profiles and oxidative stress and inflammatory markers across different age groups.

Assessing the antioxidant capacity of Ganoderma-infused white tea via electrochemical sensing techniques

This study presents a novel electrochemical method for assessing the antioxidant capacity of Ganoderma-infused white tea using carbon black-gold nanoparticle (CB-AuNP) modified glassy carbon electrodes and in situ generated superoxide radicals. The CB-AuNP nanocomposite was characterized using scanning electron microscopy and cyclic voltammetry, revealing an increased electrochemical active surface area of 0.158 cm2, which is 2.2 times larger than that of the bare glassy carbon electrode. Optimal conditions for superoxide radical generation were established using the xanthine oxidase-hypoxanthine system (0.10 U/mL XOD, 0.5 mM HX, 10 minutes incubation). The antioxidant capacity of Ganoderma-infused white tea was evaluated using cyclic voltammetry and chronoamperometry, and expressed as Trolox equivalent antioxidant capacity (TEAC). The proposed method demonstrated a strong linear relationship between TEAC values and Ganoderma extract concentration (R2 = 0.9995), with TEAC values ranging from 0.54 ± 0.03–2.85 ± 0.12 mM Trolox/g tea for 0.1–1.0 % w/v Ganoderma-infused white tea. The method exhibited good agreement with conventional spectrophotometric assays (DPPH, ABTS, and FRAP), showing strong correlations (R > 0.997) for all three assays. The practical applicability of the method was validated using commercial tea products, revealing a positive correlation between antioxidant capacity and Ganoderma polysaccharide content (R = 0.9958). The proposed electrochemical method offers a rapid, sensitive, and reliable approach for assessing the antioxidant capacity of functional tea products, with potential applications in quality control and product development.

CBD oil by-product (Hemp flakes): Evaluation for nutritional composition, heavy metals and functionality as a food ingredient

BackgroundThe recent interest among consumers in industrial hemp due to health and wellness benefits has led to several products from industrial hemp, including cannabidiol (CBD) oil. CBD oil extraction from hemp buds and flowers generates by-product biomass (hemp flakes), often posing disposal challenges and with little or no applications. We hypothesized that hemp flakes possess residual compounds with nutritional and health value that could be used to improve utilization. MethodsLocally sourced hemp flakes were compared to three commercial hemp protein products. The nutritional composition (proximate analysis), heavy metals (Al, Cu, As, Pb, Co, Cd), and functional composition (phenolic and antioxidant properties–total phenolic compounds (TPC), total flavonoid compounds (TFC), ferric reducing antioxidant potential (FRAP), 1,1–diphenyl-1-picrylhydrazyl (DPPH), Trolox equivalent antioxidant capacity (TEAC)), (CBD, cannabiodiolic acid–CBDA, cannabichromene–CBC, cannabigerol–CBG, and cannabinol–CBN) contents were determined and compared. FindingsHemp flakes had a similar nutritional composition to commercial hemp protein products, with heavy metal levels within FDA allowed limits. The by-product had significantly higher CBDA levels than commercial products. Overall, hemp flakes had comparable nutrient composition and antioxidant capabilities. Based on the protein composition of hemp flakes (31.62 %) versus the highest commercial product (43 %), hemp flakes are an acceptable functional food ingredient.

Fructooligosaccharides reverses hepatic vascular dysfunction and dysbiosis in rats with liver cirrhosis and portal hypertension.

Portal hypertension leads to lethal complications in liver cirrhosis. Oxidative stress induced hepatic vascular dysfunction, which exaggerated vasoconstriction and increases hepatic vascular resistance (HVR). Gut dysbiosis further exacerbates portal hypertension. Fructooligosaccharides are prebiotics with potent antioxidant effect. This study aimed to evaluate the roles of fructooligosaccharides in portal hypertension-related vascular dysregulation and gut microbiome. Sprague-Dawley rats received bile duct ligation to induce cirrhosis or sham operation as controls. The rats then randomly received fructooligosaccharides or vehicle for 4 weeks. Experiments were performed on the 29th day after operations. Fructooligosaccharides did not affect portal pressure. Interestingly, fructooligosaccharides significantly attenuated HVR (p = .03). Malondialdehyde, an oxidative stress marker, reduced significantly in the liver in fructooligosaccharides-treated group. In addition, superoxide dismutase and trolox equivalent antioxidant capacity increased in the treatment group. On the other hand, vasodilatation-related protein expressions, GTPCH and phospho-eNOS, enhanced significantly. Fructooligosaccharides had no adverse vasodilatation effects on splanchnic vascular system or porto-systemic collateral systems. Locomotor function was not affected by fructooligosaccharides. Faecal microbiota analysis showed that Negativicutes, Selenomonadales and Lactobacillus salivarius reduced in the fructooligosaccharides-treated group. In conclusion, fructooligosaccharides attenuate hepatic vascular dysfunction in cirrhotic rats via at least partly, ameliorate of dysbiosis and oxidative stress.

Extraction Effects on Roselle Functionalities: Antioxidant, Antiglycation, and Antibacterial Capacities.

This study investigated the effects of certain roselle (Hibiscus sabdariffa Linnaeus) extraction methods on various functional properties, including the antioxidant and antiglycation capacities and bacterial growth inhibition. Roselle anthocyanins were extracted using water and ethanol solvents at different temperatures and concentrations. The results revealed that the extraction rate increased with higher temperatures and ethanol concentrations (p < 0.05). Ethanol extracts exhibited higher total organic acid and total anthocyanin contents compared to water extracts, while water extracts showed higher total saccharide, total polyphenol, and total flavonoid contents (p < 0.05). Furthermore, the water extracts demonstrated superior Trolox equivalent antioxidant capacity (TEAC) values, while the ethanol extracts exhibited better 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging ability, antiglycation capacity, and bacterial growth inhibition. A Pearson correlation analysis revealed strong associations between specific components and functional properties, such as a positive correlation between the total anthocyanin content and antiglycation capacity (R2 = 0.9862). A principal component analysis and agglomerative hierarchical clustering highlighted distinct clusters of water and ethanol extracts, indicating solvent-dependent variations in functional properties. This study assessed roselle extraction models for antioxidant, antiglycation, and antibacterial activities, which could be used for the development of functional alcoholic or non-alcoholic beverages.

Antimicrobial and antioxidant activities of water‐soluble extracts of Camis cheeses produced by different traditional methods

AbstractTraditional Camis cheese may be an interesting dairy product with potential bioactivities and postconsumption health effects. The aim of this study was to evaluate the antimicrobial and antioxidant activity of Camis cheeses produced by two different traditional methods, differing in heat processing of the milk and boiling the curd, and to reveal the changes that occur depending on 90 days of ripening time. For this purpose, physicochemical and sensory analyses were performed, and antimicrobial and antioxidant activity analyses of the water‐soluble extracts (WSEs) of Camis cheeses (B1 and B2) were conducted. WSEs of both cheeses (800 μL mL−1) had remarkable antimicrobial effects on Escherichia coli (E. coli) and Staphylococcus aureus (Staph. aureus), with a higher inhibitory effect on E. coli. The Trolox equivalent antioxidant capacity (TEAC) values (0.12–2.24 μM Trolox) and % inhibition rates as 2,2‐diphenyl‐1‐picrylhydrazil (DPPH) radical scavenging activity (8.54–26.34%) of the cheeses indicate limited antioxidant activity. The curd boiling process was found to have a favorable effect on the antimicrobial and antioxidant activity of the Camis cheeses. However, the panelists' perceptions of flavor, texture, and total acceptability did not align with this positive outcome. The results of this study reveal that the consumption of traditional Camis cheese and its extracts or the use of purified bioactive peptides in functional food products may constitute an important alternative in terms of eliminating the negative effects encountered with the use of synthetic antimicrobial and antioxidant components to provide the desired health effect from natural sources.