Tritium Aestivum Research Articles

Review on the Effect of Seed Rate and Improved Varieties on Grain Yield of Bread Wheat (Tritium Aestivum L.) in Ethiopia

Wheat is one of the strategic crops in Ethiopia, because of its role for food security, import Substitution and supply of raw material for agro-processing industry. Wheat Produced by 4.6 million smallholder farmers on 1.8 million hectares of land with an estimated annual production of 5.0 million tons at an average productivity of 2.9 t/ha which has been consistently increasing for the last 25 years, but much lower than the world average 3.3 t/ha. The lowest yield is attributed to many factors, such as the wheat variety, seed rate, seed source, unavailability of quality seed, use of poor quality seeds and inappropriate seed size are some of the factors. Seed rate are the most important management factor affected the agronomic characteristics of wheat. Seeding rate can impact on wheat tillering, grain yield and protein quality. Hence, achieving higher agronomic performance and better end-use quality requires optimizing and periodically reviewing management practices such as seeding rates. However, in varieties that produce fewer tillers, higher seeding rates compensated for reduced tiller. In a dense wheat population, grain yield decreased due to competition between plants that induced self-regulation. So optimum seed rate is recommended depending on the growth habit of the varieties and soil fertility level for increasing wheat production and productivity.

Evaluation of Bread Wheat (Tritium aestivum L.) Genotype in Multi-environment Trials Using Enhanced Statistical Models

In varietal selection field trials, spatial variation and genotype by environment (GxE) interaction are frequent and present a major challenge to plant breeders comparing the genetic potential of several cultivars. To consistently select superior cultivars that increase agricultural production, bread wheat breeding studies must be evaluated using efficient statistical techniques. By modeling the interactions of geographical field trends and genotypes by environment interaction, this work aimed to forecast the genetic potential of bread wheat varieties across settings and improve selection tactics. The dataset utilized in this investigation consisted of sixteen multi-environment trials (MET) that were carried out using a randomized complete block design (RCBD), with two replications arranged in plot arrays of rows and columns. The findings showed that the factor analytical and spatial models were effective ways to analyze the data for this study under the linear mixed model. By ranking average Best Linear Unbiased Predictions (BLUPs) within clusters, the 16 bread wheat environments were grouped into three mega environments (C1, C2, and C3) based on yield. This served as a selection indicator. Ranking average BLUPs helped in the selection of superior and stable genotypes. The first cluster (C1)'s mean BLUP values were used to score the genotypes' performance; C2 and C3 were excluded because of their limited genetic variety and low genetic connection with the other trials. The genotypes with the highest potential based on this cluster were EBW192346 and EBW192347, chosen for a subsequent verification study to release a variety. The estimates for variance component parameters ranged from 0.013 to 3.024 for genetic variance and from 0.072 to 0.37 for error variance. Hence, scaling up the use of this efficient analysis method will improve the selection of superior bread wheat varieties.

Morphological and molecular characterization of some bread wheat (Tritium aestivum L.) genotypes

Morphological and molecular characterization of some bread wheat (Tritium aestivum L.) genotypes

Genome-wide association studies reveal stable loci for wheat grain size under different sowing dates.

Wheat (Tritium aestivum L.) production is critical for global food security. In recent years, due to climate change and the prolonged growing period of rice varieties, the delayed sowing of wheat has resulted in a loss of grain yield in the area of the middle and lower reaches of the Yangtze River. It is of great significance to screen for natural germplasm resources of wheat that are resistant to late sowing and to explore genetic loci that stably control grain size and yield. A collection of 327 wheat accessions from diverse sources were subjected to genome-wide association studies using genotyping-by-sequencing. Field trials were conducted under normal, delayed, and seriously delayed sowing conditions for grain length, width, and thousand-grain weight at two sites. Additionally, the additive main effects and multiplicative interaction (AMMI) model was applied to evaluate the stability of thousand-grain weight of 327 accessions across multiple sowing dates. Four wheat germplasm resources have been screened, demonstrating higher stability of thousand-grain weight. A total of 43, 35, and 39 significant MTAs were determined across all chromosomes except for 4D under the three sowing dates, respectively. A total of 10.31% of MTAs that stably affect wheat grain size could be repeatedly identified in at least two sowing dates, with PVE ranging from 0.03% to 38.06%. Among these, six were for GL, three for GW, and one for TGW. There were three novel and stable loci (4A_598189950, 4B_307707920, 2D_622241054) located in conserved regions of the genome, which provide excellent genetic resources for pyramid breeding strategies of superior loci. Our findings offer a theoretical basis for cultivar improvement and marker-assisted selection in wheat breeding practices.

Evaluation of different insoluble zinc sources along with zinc solubilizing bacteria on growth of wheat (Tritium aestivum) in an inceptisol having marginal Zn availability

Evaluation of different insoluble zinc sources along with zinc solubilizing bacteria on growth of wheat (Tritium aestivum) in an inceptisol having marginal Zn availability

Effects of sowing date variation on winter wheat (Tritium aestivum L.) quality and grain yield

Effects of sowing date variation on winter wheat (Tritium aestivum L.) quality and grain yield

Long-term effects of liming on crop yield, plant diseases, soil structure and risk of phosphorus leaching

This study examined the long-term effects of applying structure lime (mixture of ~80% CaCO3 and 20% Ca(OH)2) and ground limestone (CaCO3) on soil aggregate stability and risk of phosphorus (P) losses 5–7 years after liming, incidence of soil-borne diseases and yield in winter wheat (Tritium aestivum), oilseed rape (Brassica napus) and sugar beet (Beta vulgaris). Lime was applied in 13 field trials in Sweden 2013–2015 and soil characteristics and crop yield were monitored until 2021. Seedbed (0–4 cm depth) aggregate (2–5 mm size) stability was improved to the same extent with both lime treatments compared to the untreated control, sampled 5–7 years after liming. Analyses and estimations of different P fractions (total P, PO4-P, and particulate P) in leachate following simulated rainfall events on undisturbed topsoil cores sampled 6–8 years after liming revealed lower total P and particulate P concentrations in both lime treatments compared to the untreated control. Two sugar beet trial sites with soil pH ≤7.2 before liming showed an increase in sugar yield for structure lime and ground limestone as an effect of increased concentration of soil potassium (K-AL) and/or lower Aphanomyces root rot potential compared to the untreated control. The yield of winter wheat was not affected by the application of either type of lime at sites with pH >7.2 but the yield of oilseed rape decreased after the application of structure lime.

Donors of ultra-earliness for spring common wheat breeding

Knowledge of productivity and genetic control of the rate of ultra-early lines development will facilitate their use in breeding of common wheat Tritium aestivum L. for high adaptive capacity. The research focused on ultra-early lines Rico (k-65588), Rimax (k-67257) (progeny of F3 Rico × ‘Max’, k-57181), Fori 1-8 line series (progeny of F4 ‘Photon’ × Rico) and Rifor 1-13 (progeny of F7-8 Rico × ‘Forlani Roberto’), as well as on the accessions ‘Photon’ (k-55696) and ‘Forlani Roberto’ (k-42641). The varieties ‘Leningradskaya 6’ (k-64900) and ‘Leningradskaya 97’ (k-62935) were used as standards in VIR, and ‘Chelyaba 2’ (k-64379) was used in Сhelyabinsk ARI. The alleles of Vrn and Ppd genes were identified by PCR using the published allele-specific primers. Responses to vernalization (30 days at 3°C) and to a short 12-hour day were determined according to the VIR guidelines. The ultra-earliness of common wheat lines is associated with the presence of dominant alleles Vrn-A1, Vrn-B1, Vrn-D1, Ppd-B1, Ppd-D1, and possibly Eps. The shortest emergence-to-heading period at two experimental locations was noted for Rico (39.9 ± 1.49 days), which is 14.8 ± 1.22 days earlier than the development of region-adapted varieties. The Rico genotype contains Vrn-A1, Vrn-B1, Vrn-D1, Ppd-B1 and Ppd-D1. In the Rimax genotype, different alleles of the Ppd-D1 and Vrn-B1 genes were found, possibly being a result of recombination processes in Rico × ‘Max’ hybrids. Under a short photoperiod (12 hours), the Rico and Rimax genes can interact in a cumulative polymeric mode, which is not the case in long-day environments. Unlike other Rifor lines, Rifor 4 and Rifor 5, as well as ‘Forlani Roberto’, have a recessive vrn-A1a allele. However, Rifor 4 and Rifor 5 do not respond to vernalization, while ‘Forlani Roberto’ is responsive to this factor. The absence of response to vernalization in Rifor 4 and Rifor 5 possessing the recessive vrn-A1a allele is possibly provided by a complex of modifier genes along with the dominant Vrn-D1 gene, which was formed during recombination in F7-8 Rico × ‘Forlani Roberto’ hybrids. Recombinants of spring common wheat, combining ultra-earliness and relatively high ear productivity, have been identified. Grain yield per 1 m2 of new ultra-early lines in some years can reach 90% of that of the ‘Leningradskaya 97’. It is expedient to use ultra-early lines as donors of high-rate development in common wheat breeding, considering genetic peculiarities of the source lines.

Isolation and characterization of potential P- solubilizer rhizobacteria from rhizosphere of wheat (Tritium aestivum) from lower Himalayan zone of Himachal Pradesh ​

Inorganic fertilizer is an essential component of the modern agricultural system. The overuse of fertilizer brought serious problems to the present and future generations like polluted air, water, soil, degraded lands, depleted soils and increased emissions of greenhouse gases. The excessive use of chemical fertilizers from the last decade in the agricultural system of India showed harmful effects on soil structure, soil microbial diversity, soil texture and water holding capacity of soil. Therefore, the present investigation was an attempt to isolate and identify the most potent PGPR and to check their efficacy for P- solubilization. In total,48 bacterial isolates were isolated from three different sites of Hamirpur district. The total rhizobacterial population on nutrient agar medium harboured the maximum (250x105 CFU/g soil) microbial population at Deotsidh. However, population of PGPR isolates on Pikovskaya’s agar was found highest at site Deotsidh (220x105 CFU/g soil). In case of Jensen’s medium, maximum rhizobacterial populations were found highest at site Deotsidh (200x105 CFU/g soil). In the case of King’s B agar, maximum rhizobacterial population was found at site Deotsidh (230x105 CFU/g soil). Among 48 isolates, 37 isolates were found positive for P-solubilization.

Maximizing Seed Quality and Seed Yield of Bread Wheat (Tritium aestivum L.) Through Agronomic Management in Amhara Region, Northwestern Ethiopia

A study was conducted to determine the optimum seed rate and row spacing on Kekeba bread wheat seed yield and quality performance at Adet and Wonberema, Ethiopia. The study consists of field experiments that were conducted at Adet Research Center and Wonberema farmer’s field during the 2018 and 2019 cropping seasons, and laboratory experiments at the Seed Science Laboratory at Adet Research Centre. A factorial combination of eight seed rates (75, 100,125, 150, 175, 200, 225, and 250 kg.ha-1) and two-row spacing (20 cm and 30 cm) was arranged in a randomized complete block design with three replications, and laboratory experiment in a complete randomized design with four replications. The results showed that the interaction effect of seed rate and row spacing was significantly (P<0.05) affected seed yield but had non-significant (P>0.05) on physical quality, physiological, and seedling vigor. Seed rate and row spacing had a significant (P<0.05) effect on standard germination, speed of germination, vigor index-I, and vigor index-II, but a pure seed was not significantly affected by the main effects and interaction. Based on the economic analysis at Adet, the maximum seed yield (4.4 t.ha-1) was obtained from a seed rate of 125 kg.ha-1 with 20 cm row spacing, and at Wonberema the maximum seed yield (3.26 or 3.17 t.ha-1) were obtained from a seed rate of 150 or 125 kg.ha-1 with 30 cm row spacing. Therefore, a seed rate of 125 kg.ha-1 with 20 cm row spacing was recommended at Adet, and at Wonberema seed rates of 150 and 125 kg.ha-1 with 30 cm row spacing was recommended for high seed yield and quality seed production as the 1st and the 2nd options, respectively.

Genetic improvement analysis of nitrogen uptake, utilization, translocation, and distribution in Chinese wheat in Henan Province

Genetic improvement analysis of the nitrogen use efficiency (NUE) of wheat ( Tritium aestivum L.) was undertaken to determine the factors guiding NUE in order to develop new wheat cultivars with high yield, high NUE and high quality. To investigate the genetic improvement of wheat NUE and its components, 31 wheat cultivars that had been widely planted in Henan Province since 1941 were grown under high N treatment (HN) and low N treatment (LN). The results indicated that grain yield and NUE have increased significantly with cultivar development since 1941, with an annual genetic gain of 1.03% and 0.74%, respectively, under HN and LN. The significant increase in NUE mainly resulted from a significant increase in N uptake efficiency (NupE) and N utilization efficiency (NutE). NutE was the predominant component contributing to NUE under HN, while NupE was more important than NutE for NUE under LN. The improvement of NutE occurred at the expense of lower grain N content (GNC) as grain yield increased. Breeding was shown to increase N translocation (NTE) by an annual genetic gain of 1.33% and 0.33%, respectively, under HN and LN. N accumulation at the maturity stage was higher in the modern cultivars compared to the pre-modern cultivars, mainly as a result of increased N accumulation during the stem elongation phase. The N translocation capacity of the vegetative tissues increased, and the N translocation efficiency of the sheath and chaff were higher than that of the leaves. Important genetic improvement targets for high NUE include increasing pre-anthesis N assimilation ability and post-anthesis N translocation capacity. Furthermore improvements in wheat breeding should include increasing both N uptake and NHI to improve NUE and reverse the trend of declining GNC. • Higher genetic gain of grain yield and N use efficiency under better environment. • Modern cultivars had higher N accumulation, mainly raised during stem elongation phase. • N utilization efficiency increased at the expense of lower grain N content. • Enhanced N translocation and uptake was important for N use efficiency improvement.

Determination of minerals, vitamins, antinutrient and amino acid profile of pumpkin pie produced pumpkin (cucurbeta spp) puree and Wheat (tritium aestivum) Flour supplemented with Spices and Butter.

Background: Pumpkin (Cucurbita spp.), is one of the most popular vegetables consumed in the world, has been recently recognized as a functional food. Traditional crops including the pumpkins, which are rich in micronutrients, are not consumed widely by smallholder farmers in Africa. However, the cultivation of high yielding, nutrient-rich, multipurpose crops-like pumpkin is important in solving the problems of malnutrition and contributing to food security in Africa including Nigeria. Objectives: Therefore, this work aimed at producing Pumpkin pie from Pumpkin puree and wheat supplemented with some indigenous spices alongside butter. Methodology: Formulations into various formed were made using Pearson's method. The formulations were assayed for mineral, vitamin, antinutrient and amino acid composition using standard laboratory methods. A significant (P<0.05) difference was observed in Zn and Ca content of all the samples, with sample D recording the highest value for Zn (1.30±0.01) mg/g and Ca (0.47±0.01) mg/g. However, samples A and D had the least values for Na and Fe. An increasing and decreasing pattern was observed in all the vitamin contents(B1, B2, B6, B12 and C), moving from samples A through to D. Vitamin contents of samples B1 and B6 and higher than the RDA, while B2, B12 and C were below RDA. The overall antinutrient contents observed were generally low, with sample A having the lowest values for Phytate, Oxalate and tannins. The B, C and D are more enhanced in terms of essential amino acids compared to the control group (sample A). Conclusion: Pumpkin pie can be produced from pumpkin and its supplementation of the spices can boost the pie with essential amino acids, minerals, and vitamin composition. This shows the potential of using pumpkin pie as snacks in-between meals for the prevention of Protein Energy Malnutrition (PEM).

Expression analysis of hormonal pathways and defense associated genes in gamma-rays mutagenized wheat genotypes against combined stresses of spot blotch and terminal heat

Wheat (Tritium aestivum L.) productivity is severely hampered by various pathogens and changing climatic conditions. Spot blotch and terminal heat stress are the major constraints of wheat production in the eastern Gangetic plains of India. To identify novel breeding sources and to understand underlying resistance mechanisms, forty-four gamma rays mutagenized wheat genotypes, derived from three different parents were screened under favourable agro-ecological conditions for spot blotch and terminal heat stress. Ten mutants showed reduced spot blotch infection calculated based on Area Under Disease Progress Curve (AUDPC), than their respective parents. The mutant TAW41 had the least infection (AUDPC: 354.32), significantly lower than its parent HD2967 (AUDPC: 675.51) and other checks. TAW41 also had a higher Normalized Difference Vegetation Index (NDVI) and chlorophyll content than the parent. Gene expression analysis of TAW41 showed differential accumulation of transcripts involved in hormonal pathways (Salicylic acid, Jasmonic acid, and ethylene) and other defense-associated genes, indicating that TAW41 might have unique resistance mechanism that facilitates this genotype to perform better against the combined stress of spot blotch and terminal heat. Hence, mutant TAW41 has been identified as a novel source of resistance that could be exploited in wheat improvement programmes to enhance tolerance to spot blotch and terminal heat stress.

Determination of minerals, vitamins, antinutrient and amino acid profile of pumpkin pie produced pumpkin (cucurbeta spp) puree and Wheat (tritium aestivum) Flour supplemented with Spices and Butter.

ABSTRACT Background: Pumpkin (Cucurbita spp.), is one of the most popular vegetables consumed in the world, has been recently recognized as a functional food. Traditional crops including the pumpkins, which are rich in micronutrients, are not consumed widely by smallholder farmers in Africa. However, the cultivation of high yielding, nutrient-rich, multipurpose crops-like pumpkin is important in solving the problems of malnutrition and contributing to food security in Africa including Nigeria. Objectives: Therefore, this work aimed at producing Pumpkin pie from Pumpkin puree and wheat supplemented with some indigenous spices alongside butter. Methodology: Formulations into various formed were made using Pearson's method. The formulations were assayed for mineral, vitamin, antinutrient and amino acid composition using standard laboratory methods. A significant (P<0.05) difference was observed in Zn and Ca content of all the samples, with sample D recording the highest value for Zn (1.30±0.01) mg/g and Ca (0.47±0.01) mg/g. However, samples A and D had the least values for Na and Fe. An increasing and decreasing pattern was observed in all the vitamin contents(B1, B2, B6, B12 and C), moving from samples A through to D. Vitamin contents of samples B1 and B6 and higher than the RDA, while B2, B12 and C were below RDA. The overall antinutrient contents observed were generally low, with sample A having the lowest values for Phytate, Oxalate and tannins. The B, C and D are more enhanced in terms of essential amino acids compared to the control group (sample A). Conclusion: Pumpkin pie can be produced from pumpkin and its supplementation of the spices can boost the pie with essential amino acids, minerals, and vitamin composition. This shows the potential of using pumpkin pie as snacks in-between meals for the prevention of Protein Energy Malnutrition (PEM). Keywords: Butter, Cinnamon, Ginger, Pumpkin

In vitro metabolism of tebuconazole, flurtamone, fenhexamid, metalaxyl-M and spirodiclofen in Cannabis sativa L. (hemp) callus cultures.

BACKGROUND Cannabis sativa L. (hemp) is a medicinal plant producing various cannabinoids. Its consumption is legalized for medical use due to the alleged positive health effects of these cannabinoids. To satisfy the demand, C. sativa plants are propagated in contained growth chambers. During indoor propagation, pesticides usually are used to ensure efficient production. However, pesticide registration and safe application in C. sativa has not been investigated in detail.RESULTSWith this study the metabolic degradation of pesticides in recently established C. sativa callus cultures was examined. Tebuconazole, metalaxyl‐M fenhexamid, flurtamone and spirodiclofen were applied at 10 μm for 21 days. Results were compared with metabolism data obtained from Brassica napus L., Glycine max (l.) Merr., Zea mays l. and Tritium aestivum l. callus cultures as well as in metabolism guideline studies. The successfully established C. sativa callus cultures were able to degrade pesticides by oxidation, demethylation, and cleavage of ester bonds in phase I, as well as glycosylation and conjugation with malonic acid in phase II and III. Initial metabolites were detected after Day (D)7 and were traced at D21.CONCLUSIONThe resulting pathways demonstrate the same main degradation strategies as crop plants. Because metabolites could be the main residue, the exposure of consumers to these residues will be of high importance. We present here an in vitro assay for a first estimation of pesticide metabolism in C. sativa. © 2021 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry.

THE EFFECT OF PURIFIED AMOUNT OF TRITERPENE GLYCOSIDES AND THEIR ENRICHED EXTRACT FROM LEAVES OF SILPHIUM PERFOLIATUM L. ON THE GERMINATION OF SEEDS OF WINTER WHEAT AND THE ACTIVITY OF CATALASE IN THEM

The effect of purified amount of triterpene glycosides (PATG) containing, as major components, oleanolic acid glycosides – sylphiosides B, C, E, G and extract (E) enriched with them from Silphium perfoliatum L. (Asteraceae) leaves on seed germination and catalase activity (EC 1.11.1.6) in them on two varieties of winter wheat (Tritium aestivum L.) was studied. It has been shown that, the treatment of seeds with PATG solutions at concentrations of 0.0005 and 0.001% and E at concentrations of 0.2 and 0.4% increases the intensity of their swelling within 48 hours after soaking by 3.1–5.2% compared to the control, which leads to an earlier achievement of the threshold levels necessary for the activation of metabolic processes. As a result of a study of changes in catalase activity in germinating seeds of winter wheat after 1, 3 and 7 days after soaking, it was revealed that the greatest effect of seed treatment with TG preparations in manifested after 1 and 7 days of observation, its value in relation to the control respectively is 25–35% and 35–55% depending on the variety. The stimulating effect of TG preparations on catalase activity in germinating seeds of winter wheat was established for the first time. Under the influence of treatment with TG preparations the germination energy and laboratory germination of seeds increase by 3–8 and 3–6%, respectively, depending on the variety. The data obtained make it possible to consider the total preparation of TG isolated from leaves of S. perfoliatum as promising growth promoters for pre-sowing treatment of winter wheat seeds.

Comparison of antioxidant enzyme activity and gene expression in two new spring wheat cultivars treated with salinity

This study aimed to analyze element content, antioxidative response, and related gene expression in two new wheat (Tritium aestivum L.) cultivars Longchun 30 and Longchun 27 when exposed to different NaCl concentrations. Low NaCl concentration (25 mM) promoted root growth and decreased malondialdehyde (MDA) content and relative conductivity (REC) in Longchun 30. Differently, higher salinity stress (100 and 200 mM NaCl) inhibited root growth and increased MDA content and REC in both cultivars. Under salt stress, the increment of Na content in the roots and leaves and the reduction of Ca content in the roots were more remarkable in Longchun 27 than in Longchun 30. In contrast, the potassium content decreased in the roots but did not significantly change in the leaves in both cultivars under salinity. When the seedlings were exposed to salinity, the increases of superoxide dismutase (SOD) and catalase (CAT) activities in Longchun 27 roots were associated with high isoenzymes abundance and high TaCu/ZnSOD, TaMnSOD and TaCAT expression. Meanwhile, total peroxidase (POD) activity induced by NaCl treatment coincided with the changes of TaPOD expression and isoenzyme abundance in both cultivars. Besides, the inhibition of activities of apoplastic antioxidant enzymes, cell wall-bound POD, diamine oxidase, and polyamine oxidase was observed in salinity-stressed roots of both cultivars. Taken together, cv. Longchun 30 might be more suitable for growing in salinity environment in comparison with Longchun 27.

Formulation and preclinical evaluation of Anti-inflammatory activity of Triticum aestivum

A wide scope of medical advantages has been credited to wheatgrass, the young grass of the wheat plant Tritium aestivum. Wheat grass is a decent wellspring of mineral supplements. It contains critical measures of iron, phosphorous, magnesium, manganese, copper and zinc. Wheatgrass is a rich supplement of tocopherols with high vitamin E content. Wheatgrass is beneficial in restoring more infections due to its significant function that, it can arrest the development of antagonistic microbes which are responsible for spreading certain diseases. constituents of wheatgrass may be obtained from fresh juice, frozen juice, powder, tablets with compositions differing as per their production methods which otherwise depends on growing conditions of wheatgrass. Anti-inflammatory activity of wheatgrass tablets was assessed by using formalin induced rat paw edema model. The results obtained were compared with aceclofenac, standard drug.
 Keywords: Triticum aestivum, Flaxseed gel, Anti-inflammatory, Aceclofenac, Formalin.

Biochemical Analysis of Phenolics Compounds Accumulated During Developing Stages of Wheatgrass

The current study was carried out to evaluate the accumulation of phenolic compounds in distinct developmental stages of Wheatgrass (Tritium aestivum). The wheatgrass was grown in the container/tray in the standard condition in the laboratory space provided by the department. Overnight soaked seeds were then sowed in the soil containers for 25 days. The accumulation of phenolics compounds was qualitatively and quantitatively checked in the leaves at 5, 10,15,20,25 days. Phenolics compounds were sequentially extracted in methanol, n-hexane, and acetone. For qualitative analysis, various biochemical tests were carried out and phenolics compounds were quantitatively estimated by Folin-Ciocalteu reagent. Among three solvents methanol extract was found to accumulate phenolics compounds in increasing concentration at various developmental stages i.e. 5th days - 0.7135 mg/ml, 10th days 1.2614 mg/ml, 15th days- 1.1244 mg/ml; 20th days - 1.8093 mg/ml and 25th days - 4.0693 mg/ml. On the qualitative check, methanol extract was found to be positive for tannins, terpenoids, and glycosides. These phenolics could be analyzed for antidiabetic and antimicrobial activities are the future perspective of this study.

Genotype × Environment Interaction and Selection of High Yielding Wheat Genotypes for Different Wheat-growing Areas of Ethiopia

Evaluation of wheat genotypes under different environments is essential for testing stability of their performance and range of adaptations. Where, enhanced grain yield has been achieved in bread wheat (Tritium aestivum L.) through the development of adaptable, high-yielding, and rust-resistant genotypes by evaluating advanced genotypes for yield in multi-environment trials. The adaptability of a genotype over diverse environments is usually tested by the degree of its interaction with different environments under which it is grown. This study examines fifteen bread wheat genotypes for two consecutive years (2016 and 2017) across eight locations in Ethiopia. The experiment was laid out using a Randomized Complete Block design and replicated three times intending to determine high-yielding advanced genotype and release best performing genotypes as a variety for the end-user. Highly significant (P ≤ 0.001) effects of genotype, environment, and genotype by environment interaction were observed for grain yield. The AMMI analysis of variance indicated that environments accounted for 52.34% of the total sum of squares for grain yield (genotype yields ranged from 5.76 t/ha at E-11 to 1.31 t/ha at E-7). followed by interaction (22.95%) and genotypes (11.31%).The genotype ETBW8260 (G4) exhibited high mean grain yield and well performed to the tested environments. The ETBW8260 (G4) was selected as early maturing, high yielding, resistant to yellow rust and fitting for low to midland wheat growing areas and has a yield advantage over the standard Ogolcho and the local Kakaba. Both locations and genotypes are dispersed widely in all quadrants in the AMMI-1 biplot. As per the YSI Ogolcho (#15) ETBW8303 (G2), ETBW8454 (G12), ETBW8261 (G5), ETBW8406 (G10), ETBW8310 (G3), and ETBW8260 (G4), the genotypes of bread wheat were stable. Besides, the genotype ETBW8260 (G4 has highest mean yield with good stability. Therefore, after a one-year variety verification trial, ETBW8260 is released as a commercial variety in 2019 and a designated local name called “Balcha” and recommended for low-medium part of wheat production agroecology of the country.