Triticosecale Wittmack Research Articles

BIOLOGICAL POTENTIAL OF WINTER CEREALS IN THE NORTHERN TRANS-URALS, RUSSIA

Winter cereals (wheat, triticale, and rye) are the most cultivated crops in Russia, and their yield and quality results from the combination of environment and farm management. Generally, winter cereals have a much higher yield than spring cereals due to the ability to use winter moisture for growth. The latest study aimed to conduct a comparative assessment of the winter wheat, triticale, and rye according to the variability of quantitative traits under the environmental conditions of Northern Trans-Urals, Russia, during 2019–2020 and 2020–2021. Results revealed that the genotypes of winter triticale (× Triticosecale Wittmack) and winter rye (Secale cereale L.) showed improved harvests compared with winter wheat even under unfavorable environmental conditions of the Northern Trans-Urals, Russia. The chlorophyll content in the plant leaves was used as a tool for screening the genotypes of different winter cereals. Plant screening with the SPAD 502 Plus optical chlorophyll counter made it possible to determine the responses of genotypes to the heat and water stress conditions. For chlorophyll content in the flag leaf cells, intra- and inter-specific differences were observed. The fields of winter crops harvested in summer (late July to early August) can serve for growing multifunctional crops like ground cover, fodder, and green manure crops.

Early Vegetative Growth of Barley And Triticale Sown at Different Seed And Dap Fertilizer Rates under Rainfed Conditions

This study was carried out during the growing season 2020-2021 at two locations (Sumail and Zakho) to study the influence of different seeding (2,500000, and 3000,000 and 3,500000 seeds.ha-1) and DAP (0, 200, and 300 kg.ha-1) rates on some early growth characters of barley (Hordeum distichum L.) and Triticale (X Triticosecale wittmack). The experimental was designed as factorial of three factors in a randomized complete block design (RCBD) with three replications. Some early and pre harvest (flag leaf area, plant height, number of total tillers and number on fertile tillers) were measured. The results displayed significant superiority of all studied characters for Zakho location compered to Sumail location. Also, Barley surpassed Triticale crop in flag leaf area, plant height, but inferior in number of total and fertile tillers. Higher numbers of tillers were recorded in Sumail location of experiment with lower seeding rate (314.17 tillers) compared to Triticale or other seeding rate or Fertilizer treatments. DAP fertilizer treatment enhanced most of the studied traits while their effect was not reached significant level. The results recommend that the crops or varieties with fewer tillers are favorable in water limited environments and more studies are recommended mainly for Triticale crop as the studies on this crop in our areas are very rare rather than its economic importance in feeding and food processing

Early Vegetative Growth of Barley And Triticale Sown at Different Seed And Dap Fertilizer Rates under Rainfed Conditions

This study was carried out during the growing season 2020-2021 at two locations (Sumail and Zakho) to study the influence of different seeding (2,500000, and 3000,000 and 3,500000 seeds.ha-1) and DAP (0, 200, and 300 kg.ha-1) rates on some early growth characters of barley (Hordeum distichum L.) and Triticale (X Triticosecale wittmack). The experimental was designed as factorial of three factors in a randomized complete block design (RCBD) with three replications. Some early and pre harvest (flag leaf area, plant height, number of total tillers and number on fertile tillers) were measured. The results displayed significant superiority of all studied characters for Zakho location compered to Sumail location. Also, Barley surpassed Triticale crop in flag leaf area, plant height, but inferior in number of total and fertile tillers. Higher numbers of tillers were recorded in Sumail location of experiment with lower seeding rate (314.17 tillers) compared to Triticale or other seeding rate or Fertilizer treatments. DAP fertilizer treatment enhanced most of the studied traits while their effect was not reached significant level. The results recommend that the crops or varieties with fewer tillers are favorable in water limited environments and more studies are recommended mainly for Triticale crop as the studies on this crop in our areas are very rare rather than its economic importance in feeding and food processing

Breadmaking potential of winter triticale (Triticosecale Wittmack el. Camus) of Polissia ecotype

The results of the experimental work on the evaluation of the bread-making ability of new varieties of winter triticale of Polissia ecotype, which were carried out in the department of selection and seed production of grain crops of the National Science Center «Institute of Agriculture of the National Academy of Agrarian Sciences», located in Fastiv district of Kyiv region in 2016–2018, are highlighted. The elasticity (P) of the dough on average by varieties was found at the level of 67.2 mm, which corresponds to the indicator of «good filler» wheat, and by varieties ranged from 44 mm (indicator of «weak wheat») in Aristocrat variety to 87 mm («satisfactory improver») in Kotyhoroshko variety. The stretchability (L) of the dough by variety varied from 18 mm (Aristocrat) to 30 mm (Lyubomir) and was 23.5 mm on average. The stretchability of the dough is closely correlated with indicators such as C, the expansion index and the area of the average diagram (r=0.62–0.67). According to the results of calculations, the P/L ratio in winter triticale varieties of Polissia ecotype varied from 1.7 units in Volemir variety to 4.4 units in Kotygoroshko variety, and on average it was found at the level of 2.9 units, winter triticale variety is closer to the optimal value Volemir. According to the flour "strength" alveograph indicator, Solodyuk and Lubomyr winter triticale varieties of Polissia ecotype were distinguished, which reliably prevailed over Poliskyi 7 standard variety (NIR05=17) and amounted to 93 J and 76 J, respectively. The amount of «raw» gluten in winter triticale varieties ranged from 6.4% in Aristokrat variety to 15.0% in KS 9-17 sample, and on average was 11.8%. After drying, the amount of «dry» gluten varied from 2.38% to 5.04%, and averaged 3.9%, which was 33% of the amount of «raw» gluten. The highest indicator of hydration capacity (233.3%) was observed in Petrol variety compared to Polisky 7 standard variety, which significantly exceeded it by 47.6 units (NIR05=14). The physical properties of triticale gluten resemble weak wheat gluten with low elasticity. Tight triticale gluten makes it possible to bake good bread. The volume yield of loaves from triticale flour was 298 ml on average and varied from 260 ml in Polisky 7 variety to 345 ml in Fanat and Solodyuk varieties. Selected winter triticale varieties of Polissia ecotype with satisfactory baking quality, according to the wheat baking technology without adding improvers, are advisable to use in the baking industry.

Effect of cover crops residue on weed control and yield in conservation tillage tomato (Lycopersicon esculentum Mill.) production

AbstractIt has been proved that cover crops combined with minimal or no till in tomato production reduce weed pressure and simultaneously slow down soil erosion, conserve soil organic carbon, and improve soil fertility. Therefore, a study was conducted to evaluate the interaction effects of cover crops including legumes, (Vicia villosa Roth and Vicia sativa L.), and legumes mixed with grasses (Secale cereale L. and X Triticosecale Wittmack), and cover crop biomass management (incorporation of residues into soil, strip tillage and no tillage) on weed control, tomato yield, nitrogen level of tomato leaf and soil, and soil organic carbon for 2 years. Generally, cover crops reduced weed dry weight in all tillage systems, and increased tomato leaf N level compared to that of conventional methods, but soil N and organic carbon contents were not affected throughout the experiments. In 2017, the highest tomato yield (40.5 and 41.1 t ha−1) was recorded in legumes and mixed legumes‐grasses treatments with no‐till management, respectively. However, in 2018, the tomato yield decreased about 41% in the latter treatment. Totally, legume treatments in incorporation residue into soil and no‐till systems with 61.3 and 61.2 t ha−1, and mixed legumes‐grasses treatments in strip‐till system with 62.2 t ha−1, respectively, had the highest tomato yield compared to that of other treatments. It seemed legumes‐grasses treatment in the strip‐till was generally more advantageous over the no‐till. Based on results, to make possible no‐till in vegetable systems, cover crops should provide an appropriate seedbed for tomato roots in the soil.

The Production of Dual-Purpose Triticale in Arid Regions: Application of Organic and Inorganic Treatments under Water Deficit Conditions

Most of the arid and semi-arid regions, particularly in the Mediterranean area, suffer from the lack of a sufficient quantity of high-quality feed, as well as a low amount of rainfall that is unevenly distributed, resulting in the region being highly vulnerable to drought. A field experiment was carried out at the experimental station of the Faculty of Desert and Environmental Agriculture, Fuka, Matrouh University, Egypt during the winter seasons of 2018/19 and 2019/20 to study the performance of triticale (X Triticosecale Wittmack), grown under water deficit conditions, in terms of productivity and quality. The study investigated the influence of five levels of potassium fertilization (PF; 0, 43.2, 86.4, 129.6, and 172.8 kg ha−1) and ascorbic acid (AA; 0, 25, 50, 75, and 100 mg L−1) that was applied to the triticale grains before sowing and humic acid (HA; 0, 2.4, 4.8, 7.2, and 9.6 kg ha−1) that was applied as powder to the soil 21 days after sowing followed by sprinkler irrigation on triticale forage and grain production when forage was removed at variable ages at cutting (AC), determined as days after sowing (AC; 40, 65, 90, 115, and 140 DAS) on forage yield and nutritive value, in addition to the final grain yield of triticale. The experimental design was a central composite design with one replicate. Results indicated that the PF*AC interaction was significant, and it gave values of 84.78 and 238.00 g kg−1 for crude protein (CP) and degraded neutral detergent fiber (DNDF). In addition, the interaction between AA and AC was significant for CP, acid detergent fiber (ADF), 100-grain weight (100 GW), number of spikes m−2 (NSM−2), and plant height (PH). Moreover, the AC*HA interaction was significant with values of 175.17 and 247.00 g kg−1 for CP and DNDF, respectively, and 0.55 t ha−1 for grain yield (GY). Age at cutting exerted the strongest effect on the studied characteristics. It was observed that the contents of neutral detergent fiber (NDF), ADF, acid detergent lignin (ADL), and non-fiber carbohydrates (NFC) in the triticale forage significantly increased when the crop was cut at an advanced age, unlike CP, DNDF, GY, NSM−2, 100 GW, and PH that decreased with advanced AC. The highest values of 271.00, 256.00, and 268.00 g kg−1 for DNDF were obtained with higher levels of either PF, AA, or HA, respectively. However, the highest value of GY (0.97 t ha−1) was obtained with higher levels of PF*HA averaged over the two seasons. The interaction between AA*HA resulted in 393.39, 311.00, 27.13 g kg−1, and 0.94 t ha−1, for NDF, DNDF, ADL, and GY, respectively. The highest significant NDF (413.11 g kg−1) and DNDF (307.50 g ka−1) values were obtained with the application of high levels of either AA or HA. In the dual-purpose production system, it is recommended to cut the triticale crop at 65 DAS to achieve the optimum balance between forage yield and quality on the one hand, and final grain yield on the other hand. In the arid regions, application of PF, AA, and HA could help in reducing the damage caused by water deficit.

Optimizing Crop Systems: Integrating Forage Triticale into the Fallow of Peanut Monoculture in the North China Plain

Integrating a forage crop into the fallow (F) of the peanut (Arachis hypogaea L.) (P) mono-cropping system is a practical approach to provide forage yield and increase the resource use efficiency. However, little information about the comprehensive assessment of water utilization and economic benefits in the crop–livestock system exists for the North China Plain (NCP). This study aims to identify the crop rotation for optimizing water management and enhance economic benefit. The field experiment was performed over three years (2011–2014) to assess production, water utilization, and economic benefits when inserting forage triticale (X Triticosecale Wittmack) (T) into the peanut mono-cropping system. Results showed that replacing the fallow F-P cropping system with forage triticale provided a substantial amount of forage (the average of 9.8 t ha−1 per year) and enhanced the average system productivity by 85.1%. Cultivation of forage triticale during the fallow period decreased the subsequent peanut pod yield by 8.3% due to a 19.3% decline in soil water storage capacity during the sowing stage of peanut. Replacing fallow with forage triticale increased the system net income by 1016.2 US$ ha−1 and the water use efficiency (WUE) by 30.0%, while not affecting the economic efficiency of water use (EEWU), and thus can be recommended as a better option for maintaining relatively high system production, economic benefit, and WUE in NCP.

Triticale Improvement: Mining of Genes Related to Yellow Rust Resistance in Triticale Based on Transcriptome Sequencing.

Yellow (stripe) rust caused by Puccinia striiformis f. sp. tritici (Pst) is a major destructive fungal disease of small grain cereals, leading to large yield losses. The breeding of resistant varieties is an effective, sustainable way to control yellow rust. Elucidation of resistance mechanisms against yellow rust and identification of candidate genes associated with rust resistance are thus crucial. In this study, seedlings of two Triticosecale Wittmack cultivars, highly resistant Gannong No. 2 and susceptible Shida No. 1, were inoculated with Pst race CYR34. Transcriptome sequencing (RNA-seq) was then used to investigate their transcriptional responses against pathogen infection before and after the appearance of symptoms—10 and 20 days after inoculation, respectively. According to the RNA-seq data, the number of upregulated and downregulated differentially expressed genes (DEGs) in the resistant cultivar was greater than in the susceptible cultivar. A total of 2,560 DEGs commonly expressed in the two cultivars on two sampling dates were subjected to pathway analysis, which revealed that most DEGs were closely associated with defense and metabolic activities. Transcription factor enrichment analysis indicated that the expressions of NAC, WRKY, and FAR1 families were also significantly changed. Further in-depth analysis of resistance genes revealed that almost all serine/threonine-protein kinases were upregulated in the resistant cultivar. Other genes related to disease resistance, such as those encoding disease-resistance- and pathogenesis-related proteins were differentially regulated in the two cultivars. Our findings can serve as a resource for gene discovery and facilitate elucidation of the complex defense mechanisms involved in triticale resistance to Pst.

Mid-summer annual forage performance in organic, grass-fed production

Grass-fed ruminant production does not have the convenience of feeding easily-storable grains during periods of low forage availability. This study examined the forage yield, quality, and utilization of warm- and cool-season annual forages grown under organic management during the mid-summer “feed gap” period. Annual ryegrass (Lolium multiflorum Lam. cv. Tetra Brand), winter triticale (× Triticosecale Wittmack cv. common), oat (Avena sativa L. cv. Souris), millet (Panicum miliaceum L. cv. Crown Proso), corn (Zea mays L. cv. BMR84 and CM440 Canamaize), and sorghum-sudangrass (Sorghum bicolor [L.] Moench × Sorghum sudanense [Piper] Stapf cv. common) were grown in Carman, Manitoba, over 3 site-years in 2018 and 2019. Combined forage and weed dry matter (DM) yield was 7159 kg·ha−1 for sorghum-sudangrass (29% weeds), 5506 kg·ha−1 for corn (36% weeds), 4687 kg·ha−1 for oat (45% weeds), 4617 kg·ha−1 for annual ryegrass (95% weeds), 4542 kg·ha−1 for millet (28% weeds), and 2945 kg·ha−1 for winter triticale (51% weeds); significant differences in crop and weed biomass were observed. All forage systems were palatable to sheep with utilization rates from 47% to 65%. When all quality parameters were considered, corn, winter triticale, millet, and oat displayed adequate quality for mid-summer grazing, while sorghum-sudangrass had suboptimal crude protein concentrations. Direct measurements of forage quality on weeds showed that weeds did not compromise forage quality. This Canadian first study demonstrated the potential of forage production for mid-summer grazing in an organic, grass-fed regime with oat, millet, and corn resulting in the best combination of yield and quality.

Investigation of the effects of different genotypes on regeneration capacity in Triticale

Triticale (x Triticosecale Wittmack) is a synthetic amphiploid cereal that grows on about 3 million hectares in the world. It is grown mostly for forage or animal feed, although some triticale-based foods can be purchased at health food stores or are found in some breakfast cereals. Mature embryos of two triticale cultivars (Ümran Hanım and Melez 2001) were used as the resources explants. The effects of one auxin type (2,4-D) and three various concentrations, (4.0 mg/l; 8mg/l; 12.0mg/l) callus formation and plant regeneration were determined. Callus formation values were detected over 85% in three concentrations. In terms of results, the highest embryogenic callus formation rate was determined 46.91 % at 12 mg/l of Ümran Hanım dicamba. However, the lowest embryogenic callus formation rate was found with a value of 12.86% in Ümran Hanım's 2,4-D 8.0 mg/l hormone application. The highest regeneration capacity was determined at 12.09% at a dose of 12.0 mg/l of Mikham 2001 2,4-D. However, the lowest regeneration capacity was determined at 2.2% at the dose of 4 mg/l of Ümran Hanım 2,4-D. Our results displayed that auxin type and hormone dosage were very significant on the triticale mature embryos.

Comparison of Some Agronomic Character and yield of Triticale (X Triticosecale Wittmack) lines at Halabja and Qlyasan Regions of Kurdistan/Iraq

The main aim of this study was to determine the optimum line of triticale (X Triticosecale Wittmack) and best location for cultivating. The experimental design used in this study was randomized complete block design with two replications. In this study 29 lines of triticale were applied to assess some agronomic character (Plant Height (cm), No. of Days from Seeding to 50% Anthesis, No. of Days from Seeding to Physiological maturity, No. of Days from 50% Anthesis to Physiological maturity, No. of Fertile Tiller/Plant ), yield character (Grain Yield (t/ha), Biological Yield (t/ha) and Harvest Index). Plowing methods were used twice for the area of the study in each region. In Qlyasan region sowing started on 21 December and on 25 December in Halabja region. A total of 29 triticale lines developed from the hybridized winter triticale lines were used with a set name of 41ITYN and was taken from The International Maize and Wheat Improvement Center (CIMMYT). The 29 triticale lines consisted of (802, 803, 804, 805, 806, 807, 808, 809, 810, 811, 812, 813, 814, 815, 816, 817, 818, 819, 820, 821, 822, 823, 824, 825, 826, 827, 828, 829 and 830). The result illustrated that ) the maximum plant height, No. of Days from Seeding to 50% Anthess, No. of Days from Seeding to Physiological maturity, No. of Days from 50% Anthesis to Physiological maturity and No. of Fertile Tiller/Plant was observed in lines (804, 809, 830, 827 and 807). The highest agronomic character was observed under cultivation of triticale in the Halabja location. The maximum Grain Yield (t/ha), Biological Yield (t/ha) and Harvest Index was observed in lines (802, 828 and 827). Cultivation of the plant in Halabja region also gave the highest yield.

Effect of Sowing Rate and Maturity on the Yield and Nutritive Value of Triticale–Field Pea Forage Crops

Experiments were conducted over three years at Murrumburrah, in southern NSW, Australia to assess the yield and nutritive value of triticale–pea forage crops. Field pea (Pisum sativum L. cvv. Parafield and Morgan) were sown at 40 or 80 kg ha−1 in mixtures with triticale (x Triticosecale Wittmack cv. Tobruk) at 15, 30 or 45 kg ha−1 and sampled when the triticale was at the boot, anthesis, and milk stage of maturity, though lodging forced the third harvest at Murrumburrah to be abandoned. The yield, botanical composition, and forage nutritive value was determined. The yield was dependent on seasonal rainfall conditions ranging from an average of 58,326 kg ha−1 dry matter (DM) in 2009 to 19,914 kg ha−1 in 2010. The pea content was higher in Morgan compared to Parafield crops (486.4 vs. 384.8 g kg−1), and those sown at 80 kg ha−1 compared to 40 kg ha−1 (485.3 vs. 385.8 g kg−1). The crude protein (CP) content was higher when pea were sown at 80 kg ha−1 compared to 40 kg ha−1 (124.9 vs. 114.4 g kg−1 DM). Digestibility declined from 704.3 to 639.9 g kg−1 between the boot and milk harvests. Some yield, digestibility, and crude protein differences occurred due to pea variety, or due to triticale or pea sowing rates; however, these were infrequent, and the effect was minor.

Genotyping-by-sequencing and genome-wide association study reveal genetic diversity and loci controlling agronomic traits in triticale.

The genetic diversity and loci underlying agronomic traits were analyzed by the reads coverage and genome-wide association study based genotyping-by-sequencing in a diverse population consisting of 199 accessions. Triticale (× Triticosecale Wittmack) is an economically important grain forage and energy crop planted worldwide for its high biomass. Little is known about the genetic diversity and loci underlying agronomic traits in triticale. We performed genotyping-by-sequencing of 199 cultivars and mapped reads to the A, B, D, and R genomes for karyotype analysis. These cultivars could mostly be grouped into five types. Some chromosome abnormalities occurred with high frequency, such as 2D (2R) substitution, deletion of the long arm of chromosome 2D or the short arm of 5R, and translocation of the long arms of 7D/7A, the short arms of 6D/6A, or the long arms of 1D/1A. We chose only widely planted hexaploid triticale cultivars (153) for genome-wide association study. These cultivars could be divided into nine distinct groups, and the linkage disequilibrium decay was 25.4kb in this population. We identified 253 significant marker-trait associations (MTAs) on 20 chromosomes, except 7R. Twenty-one reliable MTAs were identified repeatedly over two environments. We predicted 16 putative candidate genes involved in plant growth and development using the genome sequences of wheat and rye. These results provide a basis for understanding the genetic mechanisms of agronomic traits and will benefit the breeding of improved hexaploid triticale.

Lodging resistance and feeding quality of triticale and cereal rye lines in an alpine pastoral area of P. R. China

AbstractTriticale (× Triticosecale Wittmack) is a hybrid progeny of wheat (Triticum spp.) and rye (Secale cereale L.) and exhibits significant heterosis compared with its parents. Triticale is grown in many countries as a forage crop and has broad application prospects. In this study, conducted in 2015 and 2016 at Gansu, P. R. China, the morphological characteristics of stems, the microstructural features of the second internode from the base, and the chemical components of two triticale lines and two rye lines were compared. This study found that the plant height of triticale was lower than that of rye (P < .01), whereas the triticale internode diameter was larger (P < .05). Compared with values in rye, the diameter of the second internode from the base, thickness of the stem wall, ratio of the length of the second internode to the total length, and the sheath/internode weight ratio were all greater in triticale. Examination of the anatomical structure of the second internode revealed that the basic and vascular tissues of stems of triticale were more developed than those of rye. In addition, cellulose, lignin, neutral detergent fiber, acid detergent fiber, and ash contents of triticale stems were less than those of rye, whereas the triticale soluble sugar content was greater (p < .01). Therefore, we inferred that triticale stems have greater nutritional value, palatability, lodging resistance, and production potential than rye and were suitable for planting and popularizing in alpine pastoral areas.

Triticale haploidy <i>in vitro</i> (literature review)

Triticale (× Triticosecale Wittmack) is a hybridized grain crop developed from wheat and rye crossings. Today, triticale is a multipurpose commercial grain crop with great potential as a human food and animal feed. The sown area of the new grain crop in the world reached 4 million hectares in 2018, grain production was about 14 million tons. The current climate change, the rapid evolution of pathogens, as well as the requirements of the modern market dictate the necessity for accelerated development of varieties while reducing the cost of their development. The production of double haploids makes it possible to reduce the time required for the development of homozygous lines by an average of 5–7 years. For the mass production of haploid triticale plants in vitro, there are used two methods, namely anther/microspore culture and distant hybridization followed by selective chromosome elimination of the pollinator. The most critical factors for the success of developing haploids in anther culture are a genotype, growing conditions of donor plants, a microspore development stage, stress effects on heads or anthers, and a nutrient media. Among the unresolved problems of the method are a genotypic dependence, a high incidence of albinism and a presence of aneuploids in the androgenic plant progeny. The rye genome is more often involved in chromosomal transformations than the wheat genome. Most aneuploids are nullisomics, most often on the 2R and 5R chromosomes. Nullisomic plants for 2R and 5R chromosomes have fewer number of spikelets per head and fewer number of kernels per head. In order to develop haploids by the method of selective chromosome elimination during distant hybridization, there have been successfully used such grain crops whose pollen is insensitive to Kr-genes, as maize (Zea mays L.) and wild cereal grass ‘Imperata Cylindrical’ (Imperata cylindrical L.). The advantages of the method are less genotypic dependence, absence of albino plants, genetic stability of regenerants, and reduced costs for developing haploid plants. The length of flowering period of ‘Imperata Cylindrical’ and the absence of the need to combine the timing of flowering period of the parents ensure the economy and efficiency of using this species when developing haploid triticale plants. The purpose of the current review was to characterize the methods of mass development of haploid triticale plants, to describe their advantages and disadvantages when being used in the breeding process.

First Report of Fusarium verticillioides Causing Fusariosis on Triticale Grain in Serbia.

Triticale (Triticosecale Wittmack) is a cereal crop species developed to combine positive traits of wheat and rye into a single plant (Arendt and Zannini, 2013). Due to its high protein content, favourable amino acid composition and nutritional values higher than maize, triticale is an excellent component for preparing feed for domestic animals (Đekić et al. 2019). In May 2017, discoloured spikes of triticale were observed in a field in Zemun Polje, Serbia, with a disease incidence between 15 and 20%. A total of 400 kernels obtained from discoloured spikes were surface sterilised with 1% sodium hypochlorite solution for 3 minutes and then rinsed 3 times with sterilized water and plated on potato dextrose agar (PDA) at 25°C incubated in the dark for 7 days. A total of 15 isolates of genus Fusarium on PDA formed cottony white to greyish purple colony with dark yellow to purple grey reverse and average colony. Obtained isolates were transferred to carnation leaf agar (CLA) and synthetic nutrition agar (SNA), for 7-day incubation in the dark at 25°C. Ten isolates formed "rabbit ear" monophialides which is the main characteristic of Fusarium verticillioides (Sacc.) Nirenberg. Microconidia are formed from monophialids in long chains. Their shape is oval with a flattened base, without septa (4.0-18.0 x 1.5-4.5 µm). Shape of macroconidia is slightly falcate to almost straight, (3.5-4.5 x 31-58 µm). Chlamydospores are not produced (Leslie and Summerell, 2006). The genomic DNA from one F. verticillioides isolate was extracted using the DNeasy Plant Mini kit (Qiagen, Hilden) according to the manufacturer's protocol. Molecular identification was confirmed by sequencing the internal transcribed spacer (ITS), translation elongation factor-1 alpha (EF-1α) and RPB2 (O'Donell, 2000). Sequence was deposited in GenBank as MZ664391, MZ666958 and MZ666957. This sequence was compared with the sequences of F. verticillioides strains registered in the GenBank database based on nucleotide similarity. It showed 100% identity to the sequences MT180471.1 (ITS), MN861767.1 (EF-1 α) and MT264836.1 (RPB2). Pathogenicity of all isolates was tested on 20 randomly selected triticale spikes in four replicates (Mesterházy et al. 1999). Inoculation was performed when half of the plants reached the flowering stage, by spraying with 20 ml of spore suspension (1 × 106 spores/ml from 7-day-old cultures on PDA). F. verticillioides RBG 1603 Q27 was used as a positive control. Inoculated spikes were covered with PVC bags for 48h. Discoloured spikes were observed after 3 week incubation in a greenhouse at 20°C with a 16h light/8h dark photoperiod. All inoculated spikes showed symptoms, similar to those from field infections. Control spikes were symptomless. The fungus was reisolated and was morphologically identical to the original isolates, thus completing Koch's postulates. Based on morphological, molecular and pathogenic features the isolated fungus was identified as F. verticillioides. Fusarium species infect crop plants worldwide resulting in yield loss and reduced grain quality due to mycotoxin contamination (Asam et al, 2017). In Serbia F. verticillioides was isolated from wheat and barley grains (Stanković et al., 2012) and to the best of our knowledge, this is the first report of F. verticillioides on triticale grain in Serbia.

Effects of harvest date and growth stage on triticale forages in the southwest USA: kinetics of in vitro disappearance of fiber and dry matter.

Recently, there has been interest in including triticale (X Triticosecale Wittmack) within forage programs in the southwest USA. Our objectives were to evaluate in vitro disappearance kinetics of neutral detergent fiber (NDF) and dry matter (DM) for cultivars identified during 2019 as positively or negatively deviant from typical cultivars, based specifically on regressions of 48-h in vitro disappearance of NDF on growth stage (GRST). All NDF analyses included the use of heat-stable α-amylase and sodium sulfite, as well as correction for residual ash (asNDFom). Seven triticale cultivars were established on December 18, 2019 at the University of Arizona Maricopa Agricultural Center, located near Maricopa, AZ. Forage plots were arranged in a randomized complete block design with three complete blocks (replications), and then harvested on seven dates the following late-winter and spring (February 26, March 17, April 1, April 14, April 28, May 12, and May 26). Based on a linear model, GRST was highly variable among cultivars on March 17 (44 ± 10.6), April 1 (57 ± 12.1), April 14 (67 ± 8.9), and April 28 (79 ± 7.2) compared with other harvest dates (SD ≤ 1.7). For concentrations of asNDFom, all cultivars exhibited linear (P ≤ 0.042) and quadratic (P < 0.001) polynomial contrasts in response to harvest date, and all cultivars except Merlin Max (P ≥ 0.063) exhibited at least one additional cubic or quartic effect (P ≤ 0.015). A contributing factor to the unique response by Merlin Max was the numerically greater maximum canopy height (145 ± 9.8cm) compared with the mean of all cultivars (107 ± 17.7cm), which also was associated with greater percentages of stem, as well as reduced percentages of DM partitioned within the grain head. Regressions of asNDFom disappearance after 30- or 48-h incubations on GRST indicated this was an effective independent variable (R2 ≥ 0.927), and responses were most often linear in nature. Generally, relationships for DM disappearance were quadratic, ostensibly due to the complicating effect of grain fill, but GRST was again an effective predictor variable with R2 statistics ≥ 0.852 for 12 of 14 combinations of cultivar and incubation time. Predicted percentages of digestible DM attributed to asNDFom disappearance were ≥50.3% through the fully flowered stage of growth, but digestible contributions from nonfiber components following the onset of grain fill profoundly affected overall DM digestibility among cultivars harvested at later GRST.

Effects of harvest date and growth stage on triticale forages in the southwest United States: agronomic characteristics, nutritive value, energy density, and in vitro disappearance of dry matter and fiber.

Recently, there has been increased interest in including triticale (X Triticosecale Wittmack) or other winter cereals within forage programs throughout the southwest United States. Our objectives were to screen 14 diverse triticale cultivars for agronomic and nutritive characteristics with specific emphasis on identifying normal, as well as deviant, responses to the calendar date and plant maturity for forages seeded in December and harvested from late February throughout May at Maricopa, AZ. Fourteen cultivars were established in a randomized complete block design with each cultivar represented within each of three field blocks. Plots were clean tilled and established on December 18, 2018, and then harvested at 2-wk intervals beginning on February 27 and ending May 23, 2019. Across all harvest dates, forage (N = 315) energy density (NEL) exhibited strong negative correlations with growth stage (r = -0.879), plant height (r = -0.913), head weight (r = -0.814), and estimated dry matter (DM) yield (r = -0.886) but was positively associated with percentages of leaf (r = 0.949), and weakly associated with percentages of the stem (r = 0.138). Through April 10, similar correlations were observed within individual harvest dates (N = 45) for growth stage, leaf percentage, and plant height but not for stem or head-weight percentages. Within later harvest dates, only sporadic correlations with NEL were observed. Primarily cubic regression relationships for neutral detergent fiber, acid detergent lignin, 30- and 48-h in vitro disappearance of DM and fiber, and NEL were fit for the mean or typical cultivar using both days from February 1 and growth stage as independent variables. Coefficients of determination (R2 ≥ 0.860) in all cases indicated a good fit for the polynomial models. For NEL, deviation from the typical cultivar when days from February 1 was used as the independent regression variable was largely affected by cultivar maturation rate. When the growth stage was substituted as the independent variable, plant height, stem percentage beginning at anthesis, and low grain-head percentage were associated with the maximum negative deviant cultivar (Merlin Max). The 0.23 Mcal/kg difference between maximum positive and negative deviant cultivars at a common late-boot/early-heading stage of growth suggests that some attention should be placed on cultivar selection as well as forage inventory needs and overall cropping goals.

Cold-modulated leaf compounds in winter triticale DH lines tolerant to freezing and Microdochium nivale infection: LC-MS and Raman study.

Tolerance to freezing and seedling diseases caused by Microdochium spp. is an essential trait for the wintering of triticale (×Triticosecale Wittmack) and other cereals. Preceding multi-year studies indicate that after long-term exposure to the low temperature, cereal seedlings acquire a genotype-dependent cross-tolerance to other subsequent stresses. This paper presents the first non-gel protein profiling performed via high performance liquid chromatography coupled with Mass Spectrometry as well as Fourier Transform-Raman spectroscopy measurements performed directly on leaves of triticale seedlings growing under different conditions. The research used doubled haploid lines selected from the mapping population, with extreme tolerance/susceptibility to freezing and M. nivale infection. These non-targeted methods led to the detection of twenty two proteins cold-accumulated in the most tolerant seedlings in relation to susceptible ones, classified as involved in protein biosynthesis, response to different stimuli, energy balancing, oxidative stress response, protein modification, membrane structure and anthocyanin synthesis. Additionally, in seedlings of the most freezing- and M. nivale -tolerant line, cold-hardening caused decrease of the carotenoid and chlorophyll content. Moreover, a decrease in the band intensity typical for carbohydrates as well as an increase in the band intensity characteristic for protein compounds were detected. Both studied lines revealed a different answer to stress in the characteristics of phenolic components.

Integrative Effects of Treated Wastewater and Synthetic Fertilizers on Productivity, Energy Characteristics, and Elements Uptake of Potential Energy Crops in an Arid Agro-Ecosystem

Using wastewater in agriculture is a desirable alternative source of irrigation and is gaining attraction worldwide. Therefore, this study was designed to assess the effect of treated municipal wastewater (TWW) and groundwater (GW), along with half and full doses of the recommended NPK dose on the plant growth, total biomass, gross energy, and macro- and trace element content and uptake of safflower (Carthamus tinctorius L.), canola (Brassica napus L.), and triticale (X Triticosecale Wittmack) grown in old and virgin soil as potential bioenergy crops. The results showed that crops planted in old or virgin soil irrigated with TWW had higher values of plant height, leaf area per plant, total chlorophyll content, total biomass, and gross and net energy contents compared to those irrigated with GW grown in virgin soil. Similarly, crops grown in old soil irrigated with TWW showed higher concentrations in dry matter and uptake for both macronutrients (N, P, and K) and trace elements (B, Zn, Mn, Cu, Cd, Pb, and Ni) compared to those planted in virgin soil and irrigated with GW. Furthermore, the application of the recommended half dose of NPK in old and virgin soil irrigated with TWW showed occasionally comparable results to that of a full recommended dose of NPK for most of the measured parameters. Importantly, the recommended half dose applied to old soil irrigated with TWW resulted in a significant improvement in all measured parameters compared to virgin soil irrigated with GW, along with a full recommended dose of NPK. Briefly, TWW can be used to irrigate crops grown for bioenergy purposes, since it did not pose any harmful effect for energy crops. In addition, it provides additional nutrients to soil and thus decreases the required rate of synthetic fertilizer by up to 50% without any significant decreases in the final production of crops.